- Research article
- Open Access
- Open Peer Review
GMP-grade α-TEA lysine salt: a 28-Day oral toxicity and toxicokinetic study with a 28-Day recovery period in Beagle dogs
BMC Cancervolume 16, Article number: 199 (2016)
Alpha-tocopheryloxyacetic acid (α-TEA) is a semi-synthetic derivative of naturally occurring vitamin E (alpha-tocopherol) that can be delivered via an oral route. Preclinical in vitro and in vivo data demonstrated that α-TEA is a potent anti-tumor agent with a safe toxicity profile in mice. We report a comprehensive study to evaluate the toxokinetics of good manufacturing practice (GMP)-grade α-TEA in dogs after daily oral administration for 28 days, followed by a 28-day recovery period.
Male and female beagle dogs received capsules of α-TEA Lysine Salt at doses of 100, 300, 1500 mg/kg/day. α-TEA plasma levels were determined by high-performance liquid chromatography (HPLC) with mass spectrometric detection. During the treatment, animals were observe for clinical signs, food consumption, body weight, and subjected to ophthalmoscopic, and electrocardiographic assessments. At the end of the dosing period, blood was taken and toxicokinetic analyses and histopathology assessments were performed when animals were necropsied.
Our findings showed that there was no α-TEA-related mortality or moribundity. At the highest dose, increases in white blood cells and fibrinogen levels were observed. These levels returned to normal at the end of the recovery period. Histopathological evaluation of major organs revealed no significant lesions related to α-TEA-treatment.
We demonstrate that for designing clinical trials in patients, the highest non-severely toxic dose (HNSTD) of α-TEA is 1500 mg/kg/day in Beagle dogs and this data informed the design of dose-escalation studies of α-TEA in patients with advanced cancer.
Alpha-tocopheryloxyacetic acid (α-TEA) is a semi-synthetic, non-hydrolysable ether derivative of vitamin E. Structurally, α-TEA differs from vitamin E by the replacement of the hydroxyl group at the carbon number 6 of the phenolic ring of the chroman head with an acetic acid residue linked by an ether bound . α-TEA is a cytotoxic drug that induces tumor cell death through targeting the mitochondria and by modulation of apoptosis and survival pathways . The in vivo anti-tumor activity of α-TEA has been reported in several pre-clinical tumor models , and is partially dependent on a T-cell-mediated immune response [3–6]. Although α-TEA has been evaluated as an anti-cancer agent in numerous pre-clinical tumor models [1, 7–10], efforts to translate these findings into human clinical trials are lacking. The goal of this study was to conduct a dose-escalation evaluation in an appropriate non-rodent animal species as required by the United States Food and Drug Administration (U. S. FDA) to obtain relevant toxico- and pharmaco-kinetic information in preparation for a first-in-human trial to evaluate the safety and tolerability of α-TEA in patients with advanced cancer. α-TEA lysine salt was administered at increasing doses of 100, 300 and 1500 mg/kg to male and female beagle dogs for 28 consecutive days, and then observed for 28 days after the last α-TEA dose. Complete measurements of body weight and food consumption were conducted over the treatment period. Ophthalmologic and electrocardiographic observations were assessed and clinical pathology evaluated. In this dose escalation study utilizing the lysine salt of α-TEA, we demonstrated that daily administration of α-TEA was not toxic at 1500 mg/kg body weight. The findings from this comprehensive and observational study to evaluate the pharmacology and toxicology of the α-TEA (lysine salt) in beagle dogs formed the basis for initiating a first-in-human clinical trial in patients with advanced cancer that is ongoing (NCT 02192346).
Preparation of α –Tocopheryloxyacetic acid lysine salt in capsules
In the process of manufacturing α-TEA free acid for pre-clinical development, the contract Commercial Research Organization (Ricerca Biosciences, LLC, Concord OH) made the observation that during the initial scale-up, the free acid exhibited liquid crystal properties. Therefore, a salt screen was performed, which led to the identification and selection of the lysine salt. α-TEA lysine salt (α-TEA LS) was synthesized (Fig. 1), using a modification of a previously described procedure . Briefly, α-TEA LS was prepared by reacting alpha-D-tocopherol with ethyl bromoacetate to form the ethyl ether intermediate. The ethyl ether intermediate was then reacted with potassium hydroxide to form α-TEA free acid. The lysine salt was formed by adding aqueous lysine solution to a solution of α-TEA in isopropyl alcohol. The lysine salt with its empirical formula of C37H66N2O6 and its molecular weight of 634.93 g/mol is a stable crystalline off-white powder. The dose concentration analysis was performed during the study and the test material was stable. Capsules for oral dose administration were prepared at least once weekly with appropriate amounts of bulk α-TEA placed into gelatin capsules (Pharmatek LLC, San Diego CA). Oral administration is the planned route of administration in humans. The dose of the drug is expressed as free acid using a correction factor of 1.3 to reflect salt content (calculated as ratio of lysine salt/free acid molecular weight).
Initial studies in mice were conducted to assess the efficacy of the lysine salt form of α-TEA against established tumors. Treatment of the animals (including but not limited to all husbandry, housing, and feeding conditions and euthanasia) was conducted in accordance with The Animal Welfare Act (Public Law 89-544) and the guidelines recommended in Guide for the Care and Use of Laboratory Animals (National Academy Press, Washington DC, 2011). The protocols and procedures involving the care and use of animals in the study were reviewed and approved by the Earle A. Chiles Research Institute and Ricerca Institutional Animal Care and Use Committees (IACUC).
α-TEA was formulated in mouse chow and delivered to mice bearing established 4 T1 mammary tumors, as we previously described. This anti-tumor study was followed by a toxicology study. For this purpose, both the α-TEA free acid and α-TEA LS forms were administered to naïve mice intravenously or by oral gavage. A total of 44 Beagle dogs (Canis familiaris; were acquired by the contract CRO (Ricerca Biosciences) from Marshall BioResources, North Rose, NY) for the study. Animals were as uniform in age as possible; dogs were pre-pubertal to young adults, at least 8 months of age (range of 7.8 to 8.3 months of age) and weighed between 4 and 8 kg at the start of dose administration. The frequency of administration was designed to mimic the regimen intended for human trial. α-TEA LS was administered in gelatin capsules once daily to 22 male and 22 female dogs at 100, 300 and 1500 mg/kg for 28 consecutive days. The amount for each animal was based on the most recent body weight. Control animals received equal number of empty gelatin capsules as the high dose animals. Animals were observed for viability and clinical signs, daily food consumption, body weight (once weekly or twice for high dose animals). Group assignment and dose level are shown in the Table 1. Clinical pathology, ophthalmic and electrocardiography examinations were done at the pre-dose and after the terminal dose. Eight dogs from each group (4 females and 4 males) were necropsied at the end of the dosing period (Day 29) or at the end of the recovery period (Day 57). Animals were fasted overnight prior to scheduled blood test or necropsy. When necropsied, organs were collected and preserved in 10 % neutral-buffered formalin with the exception of the testes, epididymides, and eyes; which were fixed in Modified Davidson’s Solution for 24 to 48 h, water washed, and then transferred to 10 % neutral-buffered formalin for storage. Histopathology assessments were performed. Bone marrow smears were stained with Wright-Giemsa before cytology evaluation.
Toxicokinetics studies were performed by Celerion Inc., a commercial research organization based in Lincoln, Nebraska, USA. Toxicokinetic parameters were studied on blood collected from the jugular vein of the animal (1 mL) on day 1 and on the last day of treatment. Blood samples were collected into K3EDTA-containing tubes at 1, 2, 4, 8, and 24 h following the first α-TEA administration. Following the last administration (day 28), blood collection for recovery animals was scheduled as follows: 2, 8, 24, 72, and 168 h. For the control animals (group 1), only the 8-hour sample was collected. The chosen time points were based on kinetic data gathered from pilot toxicity studies conducted in mice. Recovered plasma was stored at -70 °C until analysis.
Animal data, such as observations, body weights, food consumption, clinical pathology values, necropsy findings, and organ weights, were collected and reported electronically using Provantis™, Version 8 (Instem LSS Ltd. Staffordshire, UK). Urine was collected and analyzed by a Clinitek Atlas Urinalysis System. Data from the examination of urine sediment were entered directly into ProvantisTM. Toxicokinetics data were collected and stored in electronic notebook system LabnotesTM Client 1.18.
Sample preparation and High Performance Liquid Chromatography (HPLC) analysis
Systemic levels of α-TEA were determined using HPLC with mass spectrometry. Bioanalytical data were obtained from Celerion, Inc according to the SOPs written based on the GLP principles. Briefly, an aliquot of the extracted dog plasma was analyzed by an HPLC equipped with an AB SCIEX API 4000TM triple quadrupole mass spectrometer using an ESI source. Negative ions were monitored in the multiple reaction-monitoring (MRM) mode. Quantitation was determined using a weighted linear regression analysis (1/concentration2) of peak area ratios of the analyte and internal standard. The area under the plasma concentration-time curves, peak plasma concentration, time to achieve peak plasma concentration, and plasma terminal half-life (AUC, Cmax, Tmax, and T1/2) were determined using WinNonlin Version 6.2 (Pharsight, Mountain View CA), operating as a validated software system.
For comparative statistics, data through the Day 29 termination were evaluated using the Levene Test for homogeneity of variances and the Shapiro-Wilks Test for normality of distributions, with significance at p ≤ 0.05. Data determined to be homogeneous and of normal distribution were evaluated for analysis of variance (ANOVA). If the ANOVA verified significance at p ≤ 0.05, pairwise comparisons of each treated group with the control group were made using a parametric test, Dunnett t-test, to identify statistical differences (p ≤ 0.05). Data determined to be nonhomogeneous or of non normal distribution were evaluated using a Kruskal-Wallis Test for group factor significance. If significance (p ≤ 0.05) existed between groups, a nonparametric test, Wilcoxon with Bonferroni-Holm, was used to compare treated groups to the control group.
Anti-tumor activity of α-TEA lysine salt
We first tested the anti-tumor activity of GMP-manufactured α-TEA LS, by conducting experiments in BALB/c mice bearing established 4 T1 mammary tumors as previously described . Tumor bearing mice received (i) control diet until 5 days post-tumor cell injection, and then were switched to 0.39 % α-TEA salt diet; or (ii) 390 mg/kg body weight α-TEA LS diet. After 60 days of tumor monitoring, we found that α-TEA LS significantly inhibit tumor growth (Fig. 2a) and prolonged overall survival compared to control diet (Fig. 2b). Blood samples were collected from 3 naïve mice/group and systemic levels of α-TEA were determined by HPLC with mass spectrometry detection in order to compare the pharmacokinetics and exposure of a single dose of α-TEA LS with that of the free acid. The free acid could only be administered by gavage because it was toxic when administered by intravenous injection. However, both routes of administration could be used with the lysine salt. As shown in Fig. 3 and Table 2, when α-TEA was administered orally by gavage at the same dose level (200 mg/kg), exposure was higher with α-TEA LS compared to α-TEA free acid. The peak plasma concentration after administration (Cmax) was 2 times higher with α-TEA LS compared to the α-TEA free acid (Fig. 3). We observed a saturation phenomenon reflected by a slight decrease in Cmax following the increase in dose level from 100 mg/kg to 200 mg/kg of α-TEA free acid (Table 2). Our data indicate that the time to reach the peak plasma concentration after administration (Tmax) is 4 h with α-TEA LS versus 24 h for α-TEA free acid. The elimination half-lives was similar between 100 and 200 mg/kg α-TEA free acid and 200 mg/kg α-TEA lysine salt (54.6/50.2/54.6 h respectively).
Repeat daily dosing of α-TEA Lysine Salt did not cause gross toxicity
Several experiments were performed to evaluate the pharmacology and toxicology profile of repeated dosing of GMP-grade α-TEA LS in Beagle dogs, over a period of 28 days followed by a 28-day recovery period. Dose levels were chosen based on available data in mice  and from a preliminary dog tolerability study. Once daily, α-TEA salt was administered in gelatin capsules to male and female Beagle dogs at 100, 300 and 1500 mg/kg body weight for 28 consecutive days. Clinical signs were limited to fecal changes (decreased/discolored) and emesis in 1500 mg/kg animals on several occasions following start of dose administration and continuing until the start of recovery period. The decreased feces coincided with transient decreases in food consumption noted in several animals, and persisted upon continued dosing. α-TEA effects on food consumption at 1500 mg/kg were also reflected in the slightly decreased in body weight gain in all 1500 mg/kg males during the second and third week of dose administration and in two of six females during the second week and all the 1500 mg/kg females during the third week of administration (Fig. 4a, b). Individual body weight gain decreases were present at 300 and 100 mg/kg; however, overall group mean was comparable to controls and the changes were not considered toxicologically relevant. During the recovery period, the body weight gain in control and α-TEA-treated animals was comparable (data not shown). There was no moribundity or mortality throughout the course of treatment. There were no α-TEA-related ophthalmologic findings, but the 1500 mg/kg group showed sinus bradycardia on electrocardiograms at day 27 for the males and day 26 for the females.
Clinical pathology (erythroid, leukocytes, coagulation parameters, serum chemistry and urinalysis) of α-TEA-treated animals
At the dosing level of 100, 300, and 1500 mg/kg, no effect on erythroid or serum chemistry was observed. On day 29, white blood cell counts (neutrophils and lymphocytes) and fibrinogen levels were slightly higher in animals that received 1500 mg/kg α-TEA LS (in 2 of 4 males and 2 of 4 females) compared to their pre-dose level (Tables 3 and 4). Interestingly, at the end of the recovery period, no α-TEA-related changes in coagulation or leukocyte parameters were recorded (Tables 5). On Day 29, evaluation of serum chemistry parameters showed that the mean chloride concentration (CL), mean blood urea nitrogen concentration (BUN), and mean aspartate aminotransferase activity (AST) values for the 1500 mg/kg males group were statistically higher compared to the controls. Although not statistically different, the mean CL, mean BUN, and mean AST values for the 1500 mg/kg females were minimally higher compared to the controls. The mean total protein concentration (TPRO), mean albumin (ALB) concentration, mean albumin to globulin ratio (A/G), and mean cholesterol (CHOL) concentration values for the 1500 mg/kg females were statistically lower compared to the controls. Although not shown to be statistically different, the mean glucose concentration (GLU) value for the 1500 mg/kg females group was lower compared to the controls (Tables 6 and 7). At Day 57 (Table 8), the mean BUN concentration value for the 300 mg/kg and 1500 mg/kg males groups was higher compared to the controls. This difference was attributed to the previous anorexia seen in the 1500 mg/kg males group, but was not of such a magnitude as to be considered an indication of renal toxicity. No urinalysis changes were reported at the highest dose (1500 mg/kg).
Histology of major organs
Dogs were euthanized the day following the end of dosing period or following a 28-day recovery period (day 57) by an overdose of sodium pentobarbital followed by exsanguination. A necropsy with tissue collection was conducted and included examination of the carcass and muscular/skeletal system, orifices, cranial cavity and external surface of the brain, neck, thoracic, abdominal and pelvic cavities associated with their organs. There were few and minimal α-TEA-related histomorphologic or organ weight changes observed at the end of dose administration in the adrenal glands (1500 mg/kg females), duodenum (1500 mg/kg males), jejunum (100 and 1500 mg/kg males and 1500 mg/kg females), kidney, liver (1500 mg/kg, male), and thymus (300 and 1500 mg/kg males and 100 and 1500 mg/kg females). These findings were considered to be incidental. No α-TEA related macroscopic changes were observed at the end of dose administration or recovery phase, and there were no changes in organ weight. Interestingly, α-TEA-related histomorphologic changes were noted at 1500 mg/kg on Day 29 in the duodenum (mild, diffuse villous atrophy) at 1500 mg/kg, jejunum (minimal, focal or multifocal dilation of a crypt) at all dose levels, kidney (mild, diffuse, bilateral vacuolation of the epithelium lining the proximal tubules) at 1500 mg/kg, and liver (minimal, multifocal hepatocellular necrosis with minimal, multifocal sub-acute inflammation and moderate, diffuse vacuolation of hepatocytes) at 1500 mg/kg. At the end of recovery period, minimal focal dilation of jejunal crypt and moderate diffuse vacuolation of hepatocytes was present at 1500 mg/kg and mild diffuse depletion of lymphocytes of the thymic cortex was noted at 300 mg/kg.
Samples of blood were collected from the jugular vein of all animals at 1, 2, 4, 8, 24 h at day 1 and day 28 when animals received the test article. The validated LC-MS/MS method was used to analyze plasma α-TEA concentration. Mean plasma α-TEA concentrations in all samples obtained at 8 h post-dose from control animals on Day 1 were below the lower limit of quantitation (50.0 ng/mL), indicating no exposure of treated animals to α-TEA prior to dosing. As shown in Table 9, the mean Tmax ranged from 16.0 to 24.0 h on Day 1 and from 6.3 to 10.7 h on Day 28. Elimination half-life values were not calculated or not reported on Days 1 and 28 due to insufficient data points for the elimination phase. On Days 1 and 28, increases in Cmax and AUC0-24 were less than proportional for both males and females. On the first day of administration, for a 15-fold increase in dose from 100 to 1500 mg/kg, Cmax values increased 3.42-fold for males and 3.22-fold for females and AUC0-24 values increased 3.49-fold for males and 3.53-fold for females. On Day 28, α-TEA exposure was similar at 300 and 1500 mg/kg, this reflects a saturation phenomenon at 300 mg/kg dose level and above. For a 3-fold increase in dose from 100 to 300 mg/kg, Cmax increased 1.43-fold for males and 1.33-fold for females and AUC0-24 increased 1.44-fold for males and 1.29-fold for females. Cmax and AUC0-24 values were similar between males and females. The ratio of Cmax for males to Cmax for females at each dose level ranged from 0.84 to 0.90 on Day 1 and from 0.93 to 1.07 on Day 28. The ratio of AUC0-24 for males to AUC0-24 for females at each dose level ranged from 0.94 to 0.98 on Day 1 and from 0.91 to 1.09 on Day 28. The AUC ratios for α-TEA LS (ratio of AUC0-24 on Day 28 to the AUC0-24 on Day 1) was 9.82 for males and 10.31 for females at 100 mg/kg), 6.60 for males and 6.41 for females at 300 mg/kg, and 3.91 for males and 3.40 for females at 1500 mg/kg. Plots of α-TEA plasma concentration versus time are presented in Figs. 5 and 6 for Day 1 and Day 28, respectively. Exposure of animals on Day 28 was higher than exposure on Day 1, indicating accumulation of α-TEA with multiple dosing. The extent of accumulation was greatest at the low-dose level and decreased with increasing dose, presumably due to saturation of exposure at the mid- and high-dose levels. No sex-specific differences in toxicokinetics values could be reported.
This toxicology and pharmacokinetics study of GMP grade α-TEA was conducted to support an Investigator New Drug (IND) application to the FDA to conduct a first-in-human clinical trial in patients with advanced cancer based on the immunological and pro-apoptotic properties of α-TEA. Our results demonstrate that orally administered α-TEA LS reduces tumor growth and increases overall survival in mice bearing established tumors. This anti-tumor activity is comparable to that of α-TEA free acid that we previously reported . However, unlike α-TEA free acid, which is difficult to manufacture for human use due to its liquid crystal properties, α-TEA LS is crystalline in nature and can be delivered in aqueous form to animals. In the non-rodent dose escalation study, in which optimal conditions and timing of orally administered α-TEA LS were assessed, there were no signs of toxicity in dogs that received daily doses of up to 1500 mg/kg. No moribundity or mortality was observed at any of the dose levels tested throughout the treatment period. No gender-related differences were observed. Minor histomorphologic alterations were observed at the highest dose but these changes were mostly reversed at the end of the recovery period.
Clinical pathology changes were limited to increased white blood cell (lymphocyte and neutrophil) counts, and fibrinogen levels at 1500 mg/kg; these changes were likely related to mild inflammation and/or stress but all these findings were resolved by the end of recovery period. Although a full and complete toxicokinetic profile could not be determined from the study, there was an apparent less than dose proportional increase in AUC over the duration of the study. The extent of accumulation was greatest at the low-dose level and decreased with increasing dose, presumably due to saturation of exposure at the mid- and high-dose levels.
In summary, in this comprehensive examination of the prolonged use of GMP-grade α-TEA LS in dogs, followed by a 28-day recovery period, we report absence of any dose limiting toxicity. Our findings suggest that α-TEA is safe and tolerated and determined that the highest non-severely toxic dose (HNSTD) to be 1500 mg/kg. These findings formed the basis for initiating a first-in-human clinical trial of α-TEA in patients with advanced cancer, which is ongoing.
Alpha-Tocopheryloxyacetic free acid
- α-TEA LS:
α-TEA Lysine Salt
High Performance Liquid Chromatography
Lawson KA, Anderson K, Menchaca M, Atkinson J, Sun L, Knight V, Gilbert BE, Conti C, Sanders BG, Kline K. Novel vitamin E analogue decreases syngeneic mouse mammary tumor burden and reduces lung metastasis. Mol Cancer Ther. 2003;2(5):437–44.
Neuzil J, Dong LF, Ramanathapuram L, Hahn T, Chladova M, Wang XF, Zobalova R, Prochazka L, Gold M, Freeman R, et al. Vitamin E analogues as a novel group of mitocans: Anti-cancer agents that act by targeting mitochondria. Mol Aspects Med. 2007;28(5-6):607–45.
Hahn T, Akporiaye ET. alpha-TEA as a stimulator of tumor autophagy and enhancer of antigen cross-presentation. Autophagy. 2013;9(3):429–31.
Hahn T, Bradley-Dunlop DJ, Hurley LH, Von-Hoff D, Gately S, Mary DL, Lu H, Penichet ML, Besselsen DG, Cole BB, et al. The vitamin E analog, alpha-tocopheryloxyacetic acid enhances the anti-tumor activity of trastuzumab against HER2/neu-expressing breast cancer. BMC Cancer. 2011;11:471.
Hahn T, Jagadish B, Mash EA, Garrison K, Akporiaye ET. alpha-Tocopheryloxyacetic acid: a novel chemotherapeutic that stimulates the antitumor immune response. Breast Cancer Res. 2011;13(1):R4.
Li Y, Hahn T, Garrison K, Cui ZH, Thorburn A, Thorburn J, Hu HM, Akporiaye ET. The vitamin E analogue alpha-TEA stimulates tumor autophagy and enhances antigen cross-presentation. Cancer Res. 2012;72(14):3535–45.
Lawson KA, Anderson K, Snyder RM, Simmons-Menchaca M, Atkinson J, Sun LZ, Bandyopadhyay A, Knight V, Gilbert BE, Sanders BG, et al. Novel vitamin E analogue and 9-nitro-camptothecin administered as liposome aerosols decrease syngeneic mouse mammary tumor burden and inhibit metastasis. Cancer Chemother Pharmacol. 2004;54(5):421–31.
Dong LF, Grant G, Massa H, Zobalova R, Akporiaye E, Neuzil J. alpha-Tocopheryloxyacetic acid is superior to alpha-tocopheryl succinate in suppressing HER2-high breast carcinomas due to its higher stability. Int J Cancer. 2012;131(5):1052–8.
Hahn T, Szabo L, Gold M, Ramanathapuram L, Hurley LH, Akporiaye ET. Dietary administration of the proapoptotic vitamin E analogue alpha-tocopheryloxyacetic acid inhibits metastatic murine breast cancer. Cancer Res. 2006;66(19):9374–8.
Hahn T, Fried K, Hurley LH, Akporiaye ET. Orally active alpha-tocopheryloxyacetic acid suppresses tumor growth and multiplicity of spontaneous murine breast cancer. Mol Cancer Ther. 2009;8(6):1570–8.
Hahn T, Akporiaye ET. Repeat dose study of the novel proapoptotic chemotherapeutic agent alpha-tocopheryloxy acetic acid in mice. Anticancer Drugs. 2012;23(4):455–64.
This study was supported by funds from The Safeway Foundation and Providence Cancer Center.
The authors declare that they have no competing interests.
ETA, BC, WU, conception and designed research; TH planned research, ZA performed research; ETA, BC, WU analyzed data; BSG wrote the manuscript with contribution from the coauthors. All authors read and approved the final manuscript.