- Research article
- Open Access
4’-O-Methylbroussochalcone B as a novel tubulin polymerization inhibitor suppressed the proliferation and migration of acute myeloid leukaemia cells
BMC Cancer volume 21, Article number: 91 (2021)
Recent years, survival rates of human with high-risk acute myeloid leukaemia (AML) have not raised substantially. This research aimed to investigate the role of 4′-O-Methylbroussochalcone B, for the treatment of human AML.
Firstly, we evaluated the effects of six chalcones on AML cells activity by MTT assay. Immunofluorescence staining, tubulin polymerization assay and N,N′-ethylenebis (iodoacetamide) (EBI) competition assay were performed on ML-2 cells. Transwell and apoptosis assay were also utilized in ML-2 cells and OCI-AML5 cells. The expressions of migration-related proteins, apoptosis-related proteins and Wnt/β-catenin pathway were detected by Western Blot.
The results found six chalcones exhibited the anti-proliferative activity against different AML cell lines. Based on the results of immunofluorescence staining, tubulin polymerization assay and EBI competition assay, 4′-O-Methylbroussochalcone B was discovered to be a novel colchicine site tubulin polymerization inhibitor. 4′-O-Methylbroussochalcone B could induce apoptosis, inhibit proliferation and migration of ML-2 cells and OCI-AML5 cells. The cells were arrested in the G2-M phase by the treatment of 4′-O-Methylbroussochalcone B. In addition, 4′-O-Methylbroussochalcone B regulated MAPK and Wnt/β-catenin pathways in AML cells.
4′-O-Methylbroussochalcone B might inhibit proliferation and migration of the AML cells by MAPK and Wnt/β-catenin pathways as a tubulin polymerization inhibitor. It is promising for 4′-O-Methylbroussochalcone B to become a new drug to treat AML.
Human acute myeloid leukaemia (AML) as a hematologic malignancy is characterized by the accumulation of genetic changes in hemopoietic progenitor cells that regulate mechanisms of differentiation, proliferation and self-renewal [1, 2]. The mortality rates of human cancers in the United States have decreased by more than 50% from 1975 to 2010, and the 5-year survival rate for AML children has increased to 68% [3,4,5]. Although the therapeutic approaches of human AML have gradually improved, survival outcomes have not improved substantially and approximately more than half of children AMLs suffered from disease recurrence . Therefore, it is very necessary to develop novel anticancer drugs to treat human AML.
Microtubules as crucial elements of the cytoskeleton play significant roles in a wide range of cellular functions, such as maintenance of cell shape, division, motility, and intracellular vesicle transport . Recently, microtubules have been represented as an attractive target in anticancer drug discovery [8, 9]. Based on their effects on microtubule dynamics, microtubule-targeting agents are generally classified into two categories: microtubule-destabilizing and microtubule-stabilizing agents [10, 11]. Importantly, these microtubule-targeting agents exhibited a powerful cytotoxicity against a large number of cancer cell lines, including human AML cells [12, 13].
Natural products from medicinal plants have long been a rich resource for identifying novel anticancer agents with relatively few side effects . The seeds of Cullen corylifolium (L.) Medik. (syn. Psoralea corylifolia L.) (Leguminosae) have been used traditionally for treatment of vitiligo, gynaecological bleeding, and skin diseases .
Its seeds were reported to contain chalcones (4′-O-Methylbroussochalcone B, Broussochalcone B, Isobavachalcone, Bavachromene, Isobavachromene, and Dorsmanin A) as potentially antitumor agents [15, 16]. However, the anticancer actions of 4′-O-Methylbroussochalcone B against AML remain unclear. In this study, we detected the antiproliferative activity of 4′-O-Methylbroussochalcone B, Broussochalcone B, Isobavachalcone, Bavachromene, Isobavachromene, and Dorsmanin A (Fig. S1) against different AML cell lines. Importantly, 4′-O-Methylbroussochalcone B was identified as a potent tubulin polymerization inhibitor that inhibited proliferation and epithelial-mesenchymal transition in AML cells.
Chalcones including 4′-O-Methylbroussochalcone B (catalog no. ALB-RS-3963, ALB Technology Limited, USA), Broussochalcone B (catalog no. HY-N0231, MedChemexpress LLC, USA), Isobavachalcone (catalog no. CFN98593, ChemFaces, Wuhan, China), Bavachromene (catalog no. CFN92219, ChemFaces, Wuhan, China), Isobavachromene (catalog no. CFN92220, ChemFaces, Wuhan, China), and Dorsmanin A (catalog no. CFN96193, ChemFaces, Wuhan, China), from Cullen corylifolium were used in this study. GDM-1 cells (catalog no. BNCC307472), ML-2 cells (catalog no. BNCC353358) and CESS cells (catalog no. BNCC254816) were purchased from BeiNa Culture Collection (Shanghai, China) in May. 2019. OCI-AML5 (catalog no. ACC 247) cells was obtained from DSMZ in May. 2019. They were cultured in 89% RPMI medium, 10% FBS, 1% streptomycin and penicillin, respectively, all of which were obtained from Life Technologies (California, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St Louis, MO, USA). Annexin V-FITC /PI (catalog no. KGA108) was purchased from KeyGen Biotech (Jiangsu, China).
Cell culture and MTT assay
Cells were grown in a 5% CO2 incubator at 37 °C. We used trypsin to digest cells in logarithmic growth phase. Then, we plated the cells in 96-well plates at 5000 cells per well evenly. After cell adherence, chalcones were added in the 96-well plates for 48 h or 72 h. We added MTT reagent to each well and incubated for 2 h in the incubator. Finally, the absorbance value was measured at 490 nm after treatment with DMSO.
ML-2 cells were seeded into 6-well plates and then treated with 4′-O-Methylbroussochalcone B for 24 h. ML-2 cells were washed with phosphate buffer solution (PBS), and then fixed in 4% paraformaldehyde. After blocking for 0.5 h in 5% goat serum albumin, ML-2 cells were incubated with anti-β-tubulin for 2 h. Then, ML-2 cells were washed by PBS and labeled by 4, 6-diamidino-2-phenylindole.
Tubulin polymerization assay in vitro
An amount of 2 mg/mL tubulin was resuspended in PEM buffer, consisted of 80 mM PIPES (pH 6.9), 2 mM MgCl2, 0.5 mM EGTA, and 15% glycerol. 4′-O-Methylbroussochalcone B (1 μM, 3 μM and 6 μM) was preincubated on ice. The reaction was monitored by a spectrophotometer in absorbance at 340 nm at 37 °C.
EBI competition assay
ML-2 cells were seeded in 6-well plates for 24 h. 4′-O-Methylbroussochalcone B and colchicine treated them for 2 h respectively. Then, EBI were added. After 90 min, ML-2 cells were harvested to do Western Blot analysis. The signals were then visualized using chemiluminescent substrate (Zhengzhou research company, Zhengzhou, China).
Cell cycle assay
The samples of fixed cells were stained with 50 μg/ml Propidium iodide (PI, Sigma) in the dark for 30 min at room temperature, following the manufacturer’s protocol. Then the samples were tested by flow cytometer (Becton, Dickinson and Company, NJ). Data were analyzed via CellQuest software.
The cells (100 μl, 1 × 106 cells/ml) were suspended in the medium which contained 0.1% FBS and plated to the upper chamber of the transwell. The lower wells were incubated with the addition of the medium (600 μl per well), which included CCL25 (100 ng/ml; Cedarlane Laboratories, Burlington, Canada) and 0.1% FBS. The cells were hatched for 24 h at 37 °C with 5% CO2. Finally, the cells in the lower chamber were counted under a light microscope.
ML-2 cells and OCI-AML5 cells were seeded in 6-well culture plates and treated with certain concentration of 4′-O-Methylbroussochalcone B for 48 h. Cells were harvested and suspended in binding buffer containing Annexin V-FITC (0.5 mg/mL) and PI (0.5 mg/mL) then. After that, samples were incubated for 25 min in dark and analyzed with flow cytometry (Becton, Dickinson and Company, NJ).
Western blotting analysis
The proteins in ML-2 cells and OCI-AML5 cells lysates were resolved by polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane. Subsequently, the membranes were incubated with the primary antibodies overnight at 4 °C. Goat anti-rabbit IgG-HRP (catalog no. SA00001–2, proteintech, USA) served as the second antibodies. The signals were then visualized using chemiluminescent substrate. We detected the intensity of protein bands by the Quantity One software (Bio-rad, Richmond, California, USA).
Data are shown as the means ± standard error of the mean (SEM) from at least three independent experiments. Analysis of variance (ANOVA) together with Neumann-Keul’s multiple comparison test or Kolmogorov-Smirnov test were performed to obtain statistical information.
Antiproliferative effects of natural chalcones from Cullen corylifolium
Six chalcones (4′-O-Methylbroussochalcone B, Broussochalcone B, Isobavachalcone, Bavachromene, Isobavachromene, and Dorsmanin A) from Cullen corylifolium were evaluated for their antiproliferative effects against GDM-1 cells using the MTT (thiazolyl blue tetrazolium bromide) assay. The antiproliferative results were shown in Fig. 1a. After treatment of GDM-1 cells with 16 μM 4′-O-Methylbroussochalcone B, Broussochalcone B, Isobavachalcone, Bavachromene, Isobavachromene, and Dorsmanin A for 72 h, the cell proliferation rates were 8.14, 29.67, 57.33, 29.40, 69.00 and 36.67%, respectively. Based on the above results, 4′-O-Methylbroussochalcone B displayed the strongest antiproliferative effects among all six chalcones.
4′-O-Methylbroussochalcone B potently inhibited the proliferation against different AML cell lines
Then, we further assessed the antiproliferative actions of 4′-O-Methylbroussochalcone B against different AML cell lines. As depicted in Fig. 1b, 4‘-O-Methylbroussochalcone B exhibited the potently antiproliferative effects against GDM-1 cells, ML-2 cells, CESS cells and OCI-AML5 cells with IC50 values of 3.57 μM, 1.16 μM, 4.20 μM and 2.03 μM, respectively. It was observed that 4’-O-Methylbroussochalcone B showed the best antiproliferative effect of on ML-2 cells, followed by OCI-AML5 cells.
4′-O-Methylbroussochalcone B inhibited tubulin polymerization in vitro
To investigate whether the activities of the natural chalcones from Cullen corylifolium were related to the interactions with microtubule systems, all six chalcones were measured the inhibition of tubulin polymerization. Among six chalcones, 4′-O-Methylbroussochalcone B showed a concentration-dependent inhibition of tubulin polymerization with calculated IC50 values of 2.86 μM, which displayed the best inhibition. (Table S1).
The tubulin assembly assay measured the extent of assembly of 2 mg/mL tubulin after 60 min at 37 °C. Data are presented as mean from three independent experiments.
4′-O-Methylbroussochalcone B inhibited tubulin polymerization in ML-2 cells
To explore the effects of 4′-O-Methylbroussochalcone B on tubulin polymerization in ML-2 cells, we performed ML-2 cells treating with 4′-O-Methylbroussochalcone B at different concentrations (control, 1 μM and 2 μM) to examine its effect on microtubules reorganization. As shown in Fig. 2a, the expression of β-tubulin was suppressed in a concentration-dependent manner, indicating that 4′-O-Methylbroussochalcone B might be a potential tubulin polymerization inhibitor.
4′-O-Methylbroussochalcone B inhibited tubulin polymerization by targeting the colchicine binding site
The inhibition of 4′-O-Methylbroussochalcone B on tubulin polymerization in vitro was evaluated in this work. As shown in Fig. 2b, with the elevated 4′-O-Methylbroussochalcone B (1 μM, 3 μM, 6 μM), the increased tendency of the fluorescence intensity was obviously slowed down.
In the EBI assay, preincubation of 4′-O-Methylbroussochalcone B at 10 μM prevented the formation of EBI: β-tubulin adduct which resulting in the decrease of the adduct band in comparison with EBI treatment and 1 μM 4′-O-Methylbroussochalcone B treatment (Fig. 2c). These results indicated that 4′-O-Methylbroussochalcone B directly binds to the colchicine binding site of β-tubulin.
The molecular docking study was carried out to elucidate the binding features of 4′-O-Methylbroussochalcone B with tubulin. Since the α-tubulin and β-tubulin heterodimers are complex with different ligands in certain flexibility at the colchicine site, we performed the docking studies of 4′-O-Methylbroussochalcone B with the representative crystal structure (PDB code: 1SA0). As shown in Fig. 2d, two hydroxyl groups attaching to the chalcone scaffold could form two hydrogen bonds with the residues Val353 and Glu254. The carbonyl group on 4′-O-Methylbroussochalcone B also formed a hydrogen bond with the residue Val353. The 2-methylpent-2-ene group attaching to the phenyl fragment formed the hydrophobic interactions with the residues of Val177 and Pro325. All these results indicated that 4′-O-Methylbroussochalcone B could target tubulin.
ML-2 cells and OCI-AML5 cells were arrested in the G2-M phase by 4′-O-Methylbroussochalcone B
Given that 4′-O-Methylbroussochalcone B could markedly suppressed the tubulin polymerization in ML-2 cells and OCI-AML5 cells, we further performed cell cycles assay to explore the role of 4′-O-Methylbroussochalcone B. The cells were incubated with different concentrations (0, 2 μM and 4 μM) of 4′-O-Methylbroussochalcone B for 48 h. Based on the results in Fig. 3a and b, for ML-2 cells and OCI-AML5 cells, the percentage of the cells in G2-M phase of 2 μM and 4 μM groups was dramatically increased respectively, compared with control group. All the results suggested that ML-2 cells and OCI-AML5 cells were arrested in the G2-M phase significantly by 4′-O-Methylbroussochalcone B in a concentration-dependent manner.
4′-O-Methylbroussochalcone B inhibited migration in ML-2 cells and OCI-AML5 cells
As shown in Fig. 3c, with the addition of 4′-O-Methylbroussochalcone B, the migration of ML-2 cells and OCI-AML5 cells was evidently inhibited which was proportional to the concentration. Meanwhile, the expression level of migration related markers (N-cadherin, E-cadherin, Vimentin, Snail-1, and Slug) were detected by Western Blot. Figure 3d and e showed that 4′-O-Methylbroussochalcone B up-regulated the expression of E-Cadherin in both ML-2 cells and OCI-AML5 cells, while the expression of N-Cadherin and Vimentin were down-regulated correspondingly. The upstream transcription factor, Snail-1 and Slug were also down-regulated after treatment with 4′-O-Methylbroussochalcone B. Therefore, the results demonstrated that 4′-O-Methylbroussochalcone B could hinder the ML-2 cells and OCI-AML5 cells migration in a concentration dependent manner.
4′-O-Methylbroussochalcone B induced apoptosis in ML-2 cells and OCI-AML5 cells
To explore the apoptotic effects of 4′-O-Methylbroussochalcone B on ML-2 cells and OCI-AML5 cells, we performed apoptotic analysis with Annexin V-FITC/PI double staining and quantitated by flow cytometry. As shown in Fig. 4a, b, the percentage of apoptotic ML-2 cells (including early phase and late phase apoptosis) were increased up to 13.68 and 55.04% at the indicated concentrations (2 μM and 4 μM) compared to the control. In addition, the apoptotic rates of OCI-AML5 cells with the treatment of 4′-O-Methylbroussochalcone B at 2 μM and 4 μM were 12.84 and 24.13%. The apoptotic rates of both were concentration dependent. It illustrated that 4′-O-Methylbroussochalcone B could induce the apoptosis of ML-2 cells and OCI-AML5 cells.
4′-O-Methylbroussochalcone B regulated apoptosis-related proteins in ML-2 cells and OCI-AML5 cells
To further explore the mechanisms of 4′-O-Methylbroussochalcone B inducing apoptosis, the expression of key proteins involved the mitochondria-related apoptosis pathway was examined by Western Blot (Fig. 4c, d). Cleaved-caspase 3/9 and cleaved-parp activation were observed after treatment with 4′-O-Methylbroussochalcone B at different concentrations (0, 1 μM, 2 μM and 4 μM) in ML-2 cells and OCI-AML5 cells. The pro-apoptosis protein, Bax, was up-regulated in a concentration-dependent manner, while the anti-apoptosis protein Bcl-2 showed an opposite trend. The expression level of X-linked inhibitor of apoptosis protein (XIAP) was decreased in ML-2 cells and OCI-AML5 cells with the treatment of 4′-O-Methylbroussochalcone B. These revealed that 4′-O-Methylbroussochalcone B could induce the apoptosis of ML-2 cells and OCI-AML5 cells by regulating apoptosis-related proteins.
4′-O-Methylbroussochalcone B inhibited the MAPK signaling pathway in ML-2 cells and OCI-AML5 cells
In order to investigate whether 4′-O-Methylbroussochalcone B has an effect on MAPK signaling pathway, the expression level of related MAPK signaling proteins (p-c-Raf, p-MEK1, p-c-Jun, p-Erk, c-Myc and p-p-38) were determined in ML-2 cells and OCI-AML5 cells treated with 4′-O-Methylbroussochalcone B for 48 h. As shown in Fig. 5a and b, the expressive protein level of p-c-Raf, p-MEK1, p-c-Jun, p-Erk, c-Myc and p-p-38 were all downregulated in a concentration-dependent manner after the treatment of 4′-O-Methylbroussochalcone B. All these results indicated that 4′-O-Methylbroussochalcone B could inhibit the activation of MAPK signaling pathway in ML-2 cells and OCI-AML5 cells.
4′-O-Methylbroussochalcone B inhibited the Wnt/β-catenin signaling pathway in ML-2 cells and OCI-AML5 cells
According to the inhibitory results of proliferation and migration, it was hypothesized that 4′-O-Methylbroussochalcone B treatment might affect the Wnt/β-catenin signaling pathway. To confirm this property, the expression of related Wnt/β-catenin signaling proteins (β-catenin, Wnt 5α, P-β-catenin, TCF4 and GSK-3β) were determined. As shown in Fig. 5c and d, 4‘-O-Methylbroussochalcone B could decrease the expression level of β-catenin, Wnt 5α, P-β-catenin and TCF4. In contrast, the expression level of GSK-3β was significantly increased with the treatment of 4’-O-Methylbroussochalcone B in ML-2 cells and OCI-AML5 cells. Hence, these findings reflected that 4′-O-Methylbroussochalcone B could hinder the Wnt/β-catenin signaling pathway in ML-2 cells and OCI-AML5 cells.
Human AML is characterized by abnormal proliferation of undifferentiated and non-functional hematopoietic cells in the bone marrow . Improvements in supportive care have increased overall survival rates in younger patients (< 15 years of age) to more than 60%, however, 40% relapses requiring salvage therapy ultimately . Extensive research have revealed that chemotherapy drugs might be an effective strategy to treat human AML . Cullen corylifolium is a plant widely used in traditional Chinese medicine for its stomachic, anthelmintic, and diuretic properties . Some natural chalcones such as Corylin from Cullen corylifolium have been reported as antitumor agents . In the current study, six chalcones (4′-O-Methylbroussochalcone B, Broussochalcone B, Isobavachalcone, Bavachromene, Isobavachromene, and Dorsmanin A) from Cullen corylifolium were evaluated for their antiproliferative effects against the AML cells initially. Among all these chalcones, 4′-O-Methylbroussochalcone B displayed the best antiproliferative effects against the cells. All these antiproliferative results indicated that 4′-O-Methylbroussochalcone B have the potential to be a potently antitumor agent against acute myeloid leukemia.
Microtubules as dynamic polymers of α-tubulin and β-tubulin have been one of the best targets for developing anti-cancer drugs . These microtubules targeted agents are acted through two processes (i) inhibiting depolymerization of tubulin and (ii) inhibiting polymerization of tubulin . Now days, various binding domains have been explored that the three major binding domain of tubulin are taxol, vinca and colchicine binding domain . Tubulin inhibitors that bind to the colchicine-binding site have potential ability to treat cancer [25, 26]. Our results demonstrated that 4′-O-Methylbroussochalcone B could be a novel tubulin polymerization inhibitor. The EBI assay in ML-2 cells indicated that 4′-O-Methylbroussochalcone B can directly bind to the cochicine binding site of β-tubulin. In addition, the molecular docking study was carried out to elucidate the binding features of 4′-O-Methylbroussochalcone B with tubulin.
Microtubules play an important role in mitosis and cell division . It was reported that MDS, a kind of microtubules and the derivatives of chalcones, suppressed tubulin polymerization and arrested G2/M cell-cycle . Our results also showed that 4′-O-Methylbroussochalcone B caused G2/M cell-cycle in ML-2 cells and OCI-AML5 cells which was proportional to the concentration. Based on the reported references, tubulin polymerization inhibitors could induce apoptosis against different cancer cells . Thus, we further explored the apoptotic effects of 4′-O-Methylbroussochalcone B against ML-2 cells and OCI-AML5 cells. From the results of apoptosis assay, 4′-O-Methylbroussochalcone B could induce apoptosis and affect the expression level of apoptosis related proteins in a concentration dependent manner in ML-2 cells and OCI-AML5 cells.
Chalcone analogues notably suppressed on the migration of HUVEC cells . In this work, 4′-O-Methylbroussochalcone B inhibited the migration of ML-2 cells and OCI-AML5 cells obviously. Meanwhile, the expression level of migration related proteins were determined. The expression level of E-Cadherin was up-regulated by 4′-O-Methylbroussochalcone B, while the level of N-Cadherin and Slug was down-regulated correspondingly.
The mitogen-activated protein kinase (MAPK) cascade is a critical pathway for human cancer cell survival to drug therapy . There are four independent MAPK pathways composed of four signaling families: the MAPK/ERK family, c-Jun N-terminal kinase (JNK), p38, and Big MAP kinase-1 (BMK-1) signaling families . In order to explore the effects of 4′-O-Methylbroussochalcone B on MAPK pathway, the MAPK pathway related proteins (p-c-Raf, p-MEK1, p-c-Jun, p-Erk, c-Myc, and p-p-38) were investigated their expression level in ML-2 cells. Based on the Western Blot results, 4′-O-Methylbroussochalcone B down-regulated the expressive protein level of p-c-Jun, p-Erk, c-Myc, and p-p-38 in a concentration-dependent manner. In other words, in ML-2 cells and OCI-AML5 cells, 4′-O-Methylbroussochalcone B could inhibit the MAPK signaling pathway. The Wnt/β-catenin signaling pathway as an evolutionarily conserved and complex signaling cascade is associated with a host of physiological and pathophysiological processes, including embryonic patterning, cell proliferation, cell differentiation, angiogenesis, and cancer . In addition, Wnt/β-catenin pathway could affect migration process in some cancer cell lines . To explore the effects of Wnt/β-catenin pathway, the expression level of (β-catenin, Wnt 5α, P-β-catenin, TCF4 and GSK-3β) were determined. We found that the expression level of β-catenin, Wnt 5α and P-β-catenin were decreased, on the contrary, the expression level of GSK-3β was increased in a concentration dependent manner. In a nutshell, 4′-O-Methylbroussochalcone B inhibited Wnt/β-catenin pathway in ML-2 and OCI-AML5 cells. The role of Wnt/β-catenin pathway in the process that 4′-O-Methylbroussochalcone B inhibited migration will be studied in our next work. Based on the complexity of the etiology in leukemia and the diversity of tubulin pharmacology, we will devote ourselves to the discovery of new drugs and the research of new drug mechanisms in the future.
In conclusion, 4′-O-Methylbroussochalcone B potently inhibited proliferation and migration and expedited apoptosis in AML cells. 4′-O-Methylbroussochalcone B could affect the MAPK and Wnt/b-Catenin pathways. It might be a novel tubulin polymerization inhibitor targeting colchicine site to treat AML.
Availability of data and materials
The analyzed data sets generated during the study are available from the corresponding author on reasonable request.
Acute myeloid leukaemia
Thiazolyl blue tetrazolium bromide
Mitogen-activated protein kinase
Fetal bovine serum
- EBI N,N′:
Shang J, Chen WM, Liu S, Wang ZH, Wei TN, Chen ZZ, Wu WB. CircPAN3 contributes to drug resistance in acute myeloid leukemia through regulation of autophagy. Leuk Res. 2019;85:106198.
Sellar RS, Mehra V, Fox TA, Grigg A, Kulasekararaj A, Sarma A, de Lavallade H, McLornan D, Raj K, Mufti GJ, et al. Comparative analysis of melphalan versus busulphan T-cell deplete conditioning using alemtuzumab in unrelated donor stem cell transplantation for acute myeloid leukaemia. Br J Haematol. 2019;187(1):e20–4.
Kayser S, Levis MJ. Advances in targeted therapy for acute myeloid leukaemia. Br J Haematol. 2018;180(4):484–500.
Huang Y, Huang Z, An J, Xu Y. A novel dimeric CXCR4 antagonist synergizes with chemotherapy in acute myeloid leukaemia by mobilizing leukaemic cells from their associated bone marrow niches. Br J Haematol. 2019;187(1):e11–5.
Wen Y, Jin R, Chen H. Interactions between gut microbiota and acute childhood leukemia. Front Microbiol. 2019;10:1300.
Niu P, Yao B, Wei L, Zhu H, Fang C, Zhao Y. Construction of prognostic risk prediction model based on high-throughput sequencing expression profile data in childhood acute myeloid leukemia. Blood Cells Mol Dis. 2019;77:43–50.
Hong Y, Zhao Y, Yang L, Gao M, Li L, Man S, Wang Z, Guan Q, Bao K, Zuo D, et al. Design, synthesis and biological evaluation of 3,4-diaryl-1,2,5-oxadiazole-2/5-oxides as highly potent inhibitors of tubulin polymerization. Eur J Med Chem. 2019;178:287–96.
Kazan F, Yagci ZB, Bai R, Ozkirimli E, Hamel E, Ozkirimli S. Synthesis and biological evaluation of indole-2-carbohydrazides and thiazolidinyl-indole-2-carboxamides as potent tubulin polymerization inhibitors. Comput Biol Chem. 2019;80:512–23.
Gao F, Liang Y, Zhou P, Cheng J, Ding K, Wang Y. Design, synthesis, antitumor activities and biological studies of novel diaryl substituted fused heterocycles as dual ligands targeting tubulin and katanin. Eur J Med Chem. 2019;178:177–94.
Lee SC, Kim SH, Hoffmeister RA, Yoon MY, Kim SK. Novel Peptide-Based Inhibitors for Microtubule Polymerization in Phytophthora capsici. Int J Mol Sci. 2019;20(11):2614.
Sunil D, Kamath PR. Indole based tubulin polymerization inhibitors: An update on recent developments. Mini Rev Med Chem. 2016;16(18):1470–99.
Bernard D, Gebbia M, Prabha S, Gronda M, MacLean N, Wang X, Hurren R, Sukhai MA, Cho EE, Manolson MF, et al. Select microtubule inhibitors increase lysosome acidity and promote lysosomal disruption in acute myeloid leukemia (AML) cells. Apoptosis. 2015;20(7):948–59.
Madadi NR, Zong H, Ketkar A, Zheng C, Penthala NR, Janganati V, Bommagani S, Eoff RL, Guzman ML, Crooks PA. Synthesis and evaluation of a series of resveratrol analogues as potent anti-cancer agents that target tubulin. Medchemcomm. 2015;6(3):788–94.
Pinto D, Silva AMS. Anticancer natural Coumarins as Lead compounds for the discovery of new drugs. Curr Top Med Chem. 2017;17(29):3190–8.
Song HS, Jang S, Kang SC. Bavachalcone from Cullen corylifolium induces apoptosis and autophagy in HepG2 cells. Phytomedicine. 2018;40:37–47.
Kim DH, Li H, Han YE, Jeong JH, Lee HJ, Ryu JH. Modulation of Inducible Nitric Oxide Synthase Expression in LPS-Stimulated BV-2 Microglia by Prenylated Chalcones from Cullen corylifolium (L.) Medik. through Inhibition of I-κBα Degradation. Molecules. 2018;23(1):109.
Jin J, Mao S, Li F, Li X, Huang X, Yu M, Guo W, Jin J. A novel alkylating deacetylase inhibitor molecule EDO-S101 in combination with cytarabine synergistically enhances apoptosis of acute myeloid leukemia cells. Med Oncol. 2019;36(9):77.
Mahmud H, Ter Elst A, Scherpen FJG, Boer TM, Kampen KR, de Haas V, Guryev V, Peppelenbosch MM, Kornblau SM, de Bont E. Peptide microarray of pediatric acute myeloid leukemia is related to relapse and reveals involvement of DNA damage response and repair. Oncotarget. 2019;10(45):4679–90.
Gao M, Zhu H, Fu L, Li Y, Bao X, Fu H, Quan H, Wang L, Lou L. Pharmacological characterization of TQ05310, a potent inhibitor of isocitrate dehydrogenase 2 R140Q and R172K mutants. Cancer Sci. 2019;110(10):3306–14.
Yang YF, Zhang YB, Chen ZJ, Zhang YT, Yang XW. Plasma pharmacokinetics and cerebral nuclei distribution of major constituents of Psoraleae fructus in rats after oral administration. Phytomedicine. 2018;38:166–74.
Yang L, Yao Y, Bai Y, Zheng D, Zhou F, Chen L, Hu W, Xiang Y, Zhao H, Liu Z, et al. Effect of the isoflavone corylin from Cullen corylifolium on colorectal cancer growth, by targeting the STAT3 signaling pathway. Phytomedicine. 2020;80:153366.
Mohamed KO, Zaki I, El-Deen IM, Abdelhameid MK. A new class of diamide scaffold: design, synthesis and biological evaluation as potent antimitotic agents, tubulin polymerization inhibition and apoptosis inducing activity studies. Bioorg Chem. 2019;84:399–409.
Naaz F, Haider MR, Shafi S, Yar MS. Anti-tubulin agents of natural origin: targeting taxol, vinca, and colchicine binding domains. Eur J Med Chem. 2019;171:310–31.
Xia LY, Yang R, Zhang YL, Chu YC, Qi YL, Man RJ, Wang ZC, Wang BZ, Zhu HL. Design, synthesis, and biological evaluation of 2,3-diphenyl-cycloalkyl pyrazole derivatives as potential tubulin polymerization inhibitors. Chem Biol Drug Des. 2019;94(5):1894–904.
Yang J, Yan W, Yu Y, Wang Y, Yang T, Xue L, Yuan X, Long C, Liu Z, Chen X, et al. The compound millepachine and its derivatives inhibit tubulin polymerization by irreversibly binding to the colchicine-binding site in β-tubulin. J Biol Chem. 2018;293(24):9461–72.
Lindamulage IK, Vu HY, Karthikeyan C, Knockleby J, Lee YF, Trivedi P, Lee H. Novel quinolone chalcones targeting colchicine-binding pocket kill multidrug-resistant cancer cells by inhibiting tubulin activity and MRP1 function. Sci Rep. 2017;7(1):10298.
Li L, Jiang S, Li X, Liu Y, Su J, Chen J. Recent advances in trimethoxyphenyl (TMP) based tubulin inhibitors targeting the colchicine binding site. Eur J Med Chem. 2018;151:482–94.
Chen CW, Lee YL, Liou JP, Liu YH, Liu CW, Chen TY, Huang HM. A novel tubulin polymerization inhibitor, MPT0B206, downregulates Bcr-Abl expression and induces apoptosis in imatinib-sensitive and imatinib-resistant CML cells. Apoptosis. 2016;21(9):1008–18.
Huang X, Huang R, Wang Z, Li L, Gou S, Liao Z, Wang H. Pt(IV) complexes conjugating with chalcone analogue as inhibitors of microtubule polymerization exhibited selective inhibition in human cancer cells. Eur J Med Chem. 2018;146:435–50.
Delire B, Stärkel P. The Ras/MAPK pathway and hepatocarcinoma: pathogenesis and therapeutic implications. Eur J Clin Investig. 2015;45(6):609–23.
Zhao J, Peng W, Ran Y, Ge H, Zhang C, Zou H, Ding Y, Qi H. Dysregulated expression of ACTN4 contributes to endothelial cell injury via the activation of the p38-MAPK/p53 apoptosis pathway in preeclampsia. J Physiol Biochem. 2019;75(4):475-87.
Wang J, Zou Y, Wu X, Chen M, Zhang S, Lu X, Wang Q. DACH1 inhibits glioma invasion and tumor growth via the Wnt/catenin pathway. OncoTargets Ther. 2018;11:5853–63.
Fodde R, Brabletz T. Wnt/β-catenin signaling in cancer stemness and malignant behavior. Curr Opin Cell Biol. 2007;19(2):150–8.
Thanks for the support from the Affiliated Hospital of Jining Medical University.
This work was supported by Natural Science Foundation of Shandong Province (no. ZR2020MH690). The funding bodies did not have any influence on the design of the study, collection, analysis, interpretation of data or writing of manuscript.
Ethics approval and consent to participate
The authors confirmed that the cells don’t required ethics approval for their use.
Consent for publication
No potential conflict of interest was reported by the authors.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1: Figure S1.
Cullen corylifolium and chemical structues of natural chalcones in this study.
Additional file 2: Figure S2.
Additional file 3: Figure S3.
Additional file 4: Figure S4.
Additional file 5: Figure S5.
Additional file 6: Table S1.
Inhibition of Tubulin Polymerization
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Liu, Z., Wang, C., Wang, Y. et al. 4’-O-Methylbroussochalcone B as a novel tubulin polymerization inhibitor suppressed the proliferation and migration of acute myeloid leukaemia cells. BMC Cancer 21, 91 (2021). https://doi.org/10.1186/s12885-020-07759-4
- 4′-O-Methylbroussochalcone B
- Acute myeloid leukaemia
- Tubulin polymerization inhibitor