Fig. 5

The expression level of proteins MAPK of signaling and Wnt/β-catenin pathway was determined in ML-2 cells and OCI-AML5 cells with the treatment of 4′-O-Methylbroussochalcone B. a-b Western Blot analyzed the expression level of p-c-Raf, p-MEK1, p-Erk, p-c-Jun, c-Myc and p-p-38 in ML-2 cells and OCI-AML5 cells, full-length blots/gels are presented in Supplementary Fig. S4. c-d Western Blot analyzed the expression level of β-catenin, Wnt 5α, P-β-catenin, TCF4 and GSK-3β in ML-2 cells and OCI-AML5 cells, full-length blots/gels are presented in Supplementary Fig. S5. The data were presented as the mean ± SEM *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs the control