Fig. 2

4′-O-Methylbroussochalcone B as a novel tubulin polymerization inhibitor directly bind to the cochicine binding site of β-tubulin. a Immunofluorescence staining of β-tubulin inhibited by 4′-O-Methylbroussochalcone B in ML-2 cells; b Tubulin polymerization assay was admeasured for the inhibitory activity of 4 ‘-O-Methylbroussochalcone B on tubulin polymerization in vitro; c EBI assay combined with Western Blot analysis was used to in ML-2 cells, full-length blots/gels are presented in Supplementary Fig. S2a; d Molecular modeling study (PDB code 1SA0)