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Anti-proliferative activity of the quassinoid NBT-272 in childhood medulloblastoma cells

BMC Cancer volume 7, Article number: 19 (2007) Cite this article

Abstract

Background

With current treatment strategies, nearly half of all medulloblastoma (MB) patients die from progressive tumors. Accordingly, the identification of novel therapeutic strategies remains a major goal. Deregulation of c-MYC is evident in numerous human cancers. In MB, over-expression of c-MYC has been shown to correlate with anaplasia and unfavorable prognosis. In neuroblastoma – an embryonal tumor with biological similarities to MB – the quassinoid NBT-272 has been demonstrated to inhibit cellular proliferation and to down-regulate c-MYC protein expression.

Methods

To study MB cell responses to NBT-272 and their dependence on the level of c-MYC expression, DAOY (wild-type, empty vector transfected or c-MYC transfected), D341 (c-MYC amplification) and D425 (c-MYC amplification) human MB cells were used. The cells were treated with different concentrations of NBT-272 and the impact on cell proliferation, apoptosis and c-MYC expression was analyzed.

Results

NBT-272 treatment resulted in a dose-dependent inhibition of cellular proliferation (IC50 in the range of 1.7 – 9.6 ng/ml) and in a dose-dependent increase in apoptotic cell death in all human MB cell lines tested. Treatment with NBT-272 resulted in up to 90% down-regulation of c-MYC protein, as demonstrated by Western blot analysis, and in a significant inhibition of c-MYC binding activity. Anti-proliferative effects were slightly more prominent in D341 and D425 human MB cells with c-MYC amplification and slightly more pronounced in c-MYC over-expressing DAOY cells compared to DAOY wild-type cells. Moreover, treatment of synchronized cells by NBT-272 induced a marked cell arrest at the G1/S boundary.

Conclusion

In human MB cells, NBT-272 treatment inhibits cellular proliferation at nanomolar concentrations, blocks cell cycle progression, induces apoptosis, and down-regulates the expression of the oncogene c-MYC. Thus, NBT-272 may represent a novel drug candidate to inhibit proliferation of human MB cells in vivo.

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Background

Medulloblastomas (MB) are the most common malignant brain tumors in children and constitute 20% of all pediatric brain tumors [1]. With current treatment strategies, nearly half of all patients die from progressive tumors. Accordingly, the identification of novel therapeutic strategies remains a major goal.

The c-MYC oncoprotein plays a pivotal role as a regulator of tumorigenesis in numerous human cancers of diverse origin [2–5]. In childhood MB, c-MYC gene amplification has been demonstrated in ~8% of primary tumors [6–11]. Disparity between c-MYC gene copy number and c-MYC mRNA expression level in primary MB tumors and MB cell lines indicates the presence of alternative mechanisms to gene amplification in up-regulating c-MYC expression [12, 13]. High c-MYC mRNA expression and c-MYC gene amplification have been suggested to be indicators of poor prognosis in MB [6, 9, 11–18]. Furthermore, high c-MYC mRNA expression was demonstrated to be significantly associated with tumor anaplasia [19, 20].

Quassinoid analogues, such as bruceantin, are capable of inducing an array of biological responses [21, 22], including inhibition of protein synthesis [23]. Such an inhibition has been shown to occur via interference at the peptidyltransferase site, thus preventing peptide bond formation [24]. It has been shown in two independent studies that bruceantin is able to down-regulate c-MYC protein expression in a panel of leukemia, lymphoma, and myeloma cell lines [25, 26]. Cell lines expressing high levels of c-MYC oncoprotein were most sensitive to bruceantin-mediated effects [25]. Bruceantin has been evaluated in three separate phase I clinical trials with various types of solid tumors [27–29]. Side effects were relatively few and included hypotension, nausea, vomiting, and moderate hematological toxicity. However, in two phase II clinical trials bruceantin failed to prove effective in metastatic breast carcinoma [30] and in advanced malignant melanoma [31].

Based on the studies with bruceantin, proprietary quassinoid analogues have been designed and their in vitro cytotoxic activities have been compared with bruceantin by using the MTT assay in a panel of cell lines. The lipophilic small molecule NBT-272 was found to be 2–10 fold more potent than bruceantin in a variety of cancer cell lines [32]. In neuroblastoma – an embryonal tumor with biological similarities to MB – the quassinoid NBT-272 has been demonstrated not only to inhibit cellular proliferation but also to down-regulate c-MYC protein expression [32]. In the current study, we examined the effects of NBT-272 in human MB cell lines expressing different levels of c-MYC.

Methods

Human MB cell lines

DAOY (wild-type), DAOY V11 (empty vector transfected) and DAOY M2 (c-MYC vector transfected) human MB cells have been described previously [20]. D341 and D425 human MB cells were the kind gift of Dr Henry Friedman, Duke University, Durham, NC, USA. All MB cells were cultured in Richter's zinc option medium/10% fetal bovine serum (non-essential amino acids were added to the medium of D341 and D425 cells to a final concentration of 1%, and G418 was added to the medium of DAOY V11 and DAOY M2 to a concentration of 500 μg/ml). All cell cultures were maintained at 37°C in a humidified atmosphere with 5% CO2.

Real-time quantitative polymerase chain reaction

106 cells growing in their mid-log phase were treated with NBT-272 at concentrations indicated and harvested after 24 h. Total RNA isolation, reverse transcription reactions, and RT-PCR were performed as described previously [12, 33]. Kinetic real-time PCR quantification of c-MYC mRNA was performed using the ABI Prism 7700 Sequence Detection System (Applied Biosystems, Rotkreuz, Switzerland), as described previously [34]. Primers and probes for c-MYC and the endogenous control 18S rRNA were purchased from Applied Biosystems (Rotkreuz, Switzerland). For each PCR run, a master mix was prepared containing 200 nM of each primer, 400 nM probe, and 500 ng cDNA in a final volume of 25 μl. The thermal cycling conditions comprised an initial denaturation step at 95°C for 10 min, and 50 cycles at 95°C for 15 s and 60°C for 1 min. Experiments were performed in triplicate for each data point. Relative expression of c-MYC mRNA was calculated by using the comparative CT method [35].

Western blot analysis

The expression of c-MYC protein was assessed by Western blot analysis. Briefly, 106 cells growing in their mid-log phase were treated with NBT-272 at concentrations indicated and harvested after 24 h and whole-cell pellets were lysed with lysis buffer (1 ml/107 cells, 50 mM Tris-HCl buffer [pH 8.0], 150 mM NaCl, 1% (w/v) Nonidet P40, 0.1% (w/v) sodium deoxycholate, 0.1% (v/w) sodium dodecylsulfate, 1 mM EDTA, and 1 mM EGTA) containing protease inhibitors (Complete, Roche; Basel, Switzerland) and incubated on ice for 30 min. After measuring the protein concentration by the BCA method (Pierce; Rockford, USA), 12 μg total protein lysate was separated by 10 % SDS polyacrylamide gels and the gels were subjected to immunoblotting. Nonspecific binding sites were blocked by 3 h incubation in TBST (10 mM Tris pH 8.0, 150 mM NaCl, 0.05% (w/v) Tween 20) supplemented with 5% (w/v) nonfat milk powder. Membranes were incubated overnight at 4°C with a 1:2000 dilution of c-MYC polyclonal primary antibody (Santa Cruz Biotechnology; Heidelberg, Germany). Membranes were then washed three times at room temperature in TBST for 30 min each time, and bound Ig was detected using anti-isotype monoclonal secondary antibody coupled to horseradish peroxidase (Santa Cruz Biotechnology; Heidelberg, Germany). The signal was visualized by enhanced chemiluminescence ECL (Amersham Biosciences; Dübendorf, Switzerland) and autoradiography. Then, immunoblotting with a 1:5000 dilution of a monoclonal primary β-actin antibody (Sigma; Basel, Switzerland) was performed, to verify equivalent amounts of loaded protein. For densitometry, the zymographic profiles of the gels were scanned. Relative band intensities were determined using Quantity One analysis software (Bio-Rad).

c-MYC transcription factor binding activation assay

Nuclear protein extracts were obtained from human MB cells by using the BD™ TransFactor Extraction Kit (BD Clontech, Basel, Switzerland) according to the manufacturer's instructions. Aliquots of nuclear protein extracts were stored at -80°C. The activation of c-MYC was measured by using the Mercury TransFactor assay (BD Clontech, Basel, Switzerland), an enzyme-linked immunosorbent assay (ELISA)-based assay [36], according to the manufacturer's instructions. Briefly, 5 μg of nuclear protein samples were incubated for 1 h in a 96-well plate coated with an oligonucleotide which codes for the c-MYC consensus binding site sequence and to which c-MYC contained in nuclear extracts specifically binds. After washing, antibody directed against c-MYC DNA complex (1:1000 dilution) was added to these wells and incubated for 1 h. Following incubation for 1 h with a secondary horseradish peroxidase-conjugated antibody (1:1000 dilution), specific binding was detected by colorimetric estimation at 450 nm using either mutant DNA or no protein addition controlled for nonspecific binding.

Cytotoxicity assay

Exponentially growing human MB cells (5 × 103 cells/well) were cultured in 96-well plates in the presence of different concentrations of NBT-272 for 24 h. Untreated cells were used as controls. A colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay (Promega; Wallisellen, Switzerland) was used to quantitate cell viability as previously described [37]. Briefly, 100 μl of target cell suspension was added to each well of 96-well microtiter plates, and each plate was incubated at 37°C in a humidified 5% CO2 atmosphere. Following incubation, 10 μl of MTS working solution was added to each culture well, and the cultures were incubated for 4 h at 37°C in a humidified 5% CO2 atmosphere. Each experiment was performed in triplicate. The absorbance values of each well were measured with a microplate spectrophotometer (Molecular Devices; Sunnyvale, CA, USA) at 490 nm. IC50 values were calculated by using the GraphPad Prism software.

Apoptosis assay

Exponentially growing human MB cells were exposed to different concentrations of NBT-272 for 24 h. Untreated cells were used as controls. A photometric enzyme-immunoassay (Cell Death Detection ELISA; Roche Diagnostics, Basel, Switzerland) was used for the quantitative determination of cytoplasmic histone-associated DNA fragments, as described previously [37]. In brief, cell lysates of control and NBT-272-treated cells were placed in a StreptAvidin-coated microtiter plate. A mixture of biotin-labeled monoclonal histone antibody and peroxidase-conjugated monoclonal DNA antibody was then added, followed by incubation for 2 h. After washing to remove unbound antibodies, the amount of nucleosomes was quantified photometrically. The cell death nucleosomes enrichment factor was calculated by dividing the absorbance of treated cells by the absorbance of untreated control cells.

Cell cycle analysis

The percentages of cells in the different phases of the cell cycle were determined by evaluating DNA content according to methods described [38]. To arrest cells at the G1/S border, DAOY, D341, and D425 MB cells were synchronized in a medium containing 2 mM hydroxyurea (Sigma) for 14 h as described previously [39]. Cells were then transferred into fresh, hydroxyurea-free medium, or medium containing 0.4 μg/ml NBT-272. Control untreated or NBT-272 treated cells were harvested 0, 8, 16, and 24 h after release from hydroxyurea. After washing twice in PBS 1×, the cells were stained with a solution containing 50 μg/ml propidium iodide (Becton-Dickinson; Allschwil, Switzerland) and 100 U/ml RNase A (Qiagen; Hombrechtikon, Switzerland) in PBS 1× for 30 min at room temperature. A total of 30'000 events per sample were acquired. Flow cytometric analysis was performed on a FACSCalibur flow cytometer (BD Biosciences; Allschwil, Switzerland) with CELLQuest software (BD Biosciences). The percentages of the cells in the different phases of the cell cycle were calculated on linear PI histograms using the mathematical software ModFit LT 2.0 (Verity Software House; Topsham, ME, USA).

Statistical analysis

All data are expressed as mean ± SD. Student's t-test and one-way ANOVA were used to test statistical significance. P < 0.05 was considered to be significant. GraphPad Prism 4 software (San Diego, CA, USA) was used to calculate IC50 concentrations and to compare the fitted midpoints (log IC50) statistically.

Results

c-MYC expression in human MB cell lines

To test the effects of NBT-272 on human MB cells, we selected three human MB cell lines with different levels of c-MYC expression [12, 13]. DAOY human MB cells harbor a single copy of c-MYC and display low c-MYC mRNA and protein expression. In contrast, D341 and D425 human MB cells are c-MYC amplified and express high c-MYC mRNA and protein. To study a potential influence of c-MYC expression on cell responses to NBT-272 more specifically, we included DAOY human MB cells transfected to express different levels of c-MYC (DAOY wild-type, DAOY transfected with empty vector, and DAOY transfected with c-MYC) [20]. By using RT-PCR analysis, the c-MYC mRNA expression level was found to be ~20-fold higher in DAOY M2 compared to DAOY wt, or DAOY V11 (Figure 1A). D341 and D425 displayed even higher c-MYC mRNA expression levels (Figure 1A). Using Western blot analysis, c-MYC protein expression was found to be 4-fold higher in DAOY M2 compared to DAOY wt, or DAOY V11. D341 had similar c-MYC protein expression to DAOY M2, whereas D425 had higher c-MYC protein expression (Figure 1B).

Figure 1
figure 1

c-MYC mRNA (A) and protein (B) expression of the MB cell lines determined by quantitative RT-PCR and Western blot analysis. Values represent the fold-increase of c-MYC mRNA (n = 3; ± SD) and c-MYC protein expression (representative of two to three independent experiments) relative to the DAOY wt cells.

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NBT-272 treatment results in dose-dependent suppression of MB cell proliferation

To test whether treatment with NBT-272 alters proliferation of MB cells, we incubated the five human MB cell lines (DAOY wt, DAOY V11, DAOY M2, D341, and D425) with various concentrations of NBT-272 for 24 h and assessed cell viability by using the MTS assay (Figure 2). Treatment with NBT-272 resulted in a dose-dependent cytotoxic response in all MB cell lines tested with an IC50 at NBT-272 concentration of 1.7 – 9.6 ng/ml (D341, 1.7 ng/ml; D425, 4.3 ng/ml; DAOY wt, 8.7 ng/ml; DAOY V11, 9.6 ng/ml; DAOY M2, 4.7 ng/ml) (Figure 2). All MB cells were sensitive to NBT-272 treatment, and their IC50 values were in the low nanomolar range. c-MYC over-expressing DAOY M2 cells, were slightly more sensitive to NBT-272 treatment compared to DAOY wt or DAOY V11 respectively (DAOY M2, IC50: 4.7 ng/ml vs. DAOY V11, IC50: 9.6 ng/ml, p = 0.15). However, these differences were statistically not significant.

Figure 2
figure 2

NBT-272 mediated inhibition of cellular proliferation as determined by the MTS assay. Cells were incubated for 24 h with various concentrations of NBT-272 or with solvent only. Values represent the mean percentage of viability (representative from two independent experiments) compared with solvent only treated cells ± SD (n = 3; ± SD).

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NBT-272 treatment of human MB cells results in down-regulation of c-MYC protein expression

We then determined whether NBT-272 treatment down-regulates c-MYC expression. NBT-272 (0.04 or 0.4 μg/ml) treatment for 24 h resulted in no consistent down-regulation of c-MYC mRNA expression as determined by real time RT-PCR (Figure 3A). However, at the protein level, the effects of NBT-272 were clearly detectable. NBT-272 (0.4 μg/ml) treatment for 24 h resulted in a decrease in c-MYC protein level to 47% in DAOY wt, 11% in DAOY V11, 21% in DAOY M2, 13% in D341, and 9% in D425 (Figure 3A, B). These results indicate that NBT-272 has only minor effects on the c-MYC mRNA levels, but mainly acts at the level of c-MYC protein expression.

Figure 3
figure 3

NBT-272 treatment of human MB cells results in down-regulation of c-MYC protein. NBT-272 treatment (24 h) of MB cells resulted in no consistent changes of c-MYC mRNA expression as determined by quantitative RT-PCR (A), but in a decrease of c-MYC protein expression as determined by Western blot analysis (A, B). Values represent the percentage of c-MYC mRNA (n = 3; ± SD) and c-MYC protein expression (representative from two independent experiments) compared with solvent only treated cells.

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NBT-272 treatment results in a reduction of c-MYC binding activity

To examine whether treatment with NBT-272 alters c-MYC binding activity, we incubated the five human MB cell lines with NBT-272 at various concentrations (0, 0.04, and 0.4 μg/ml) for 24 h and measured binding activity. NBT-272 (0.4 μg/ml) treatment for 24 h reduced c-MYC binding activity significantly in all MB cells tested (Figure 4).

Figure 4
figure 4

NBT-272 treatment of human MB cells results in reduced c-MYC binding activity. NBT-272 treatment (24 h) of MB cells resulted in a reduction of c-MYC binding activity as determined by the ELISA-based TransAM-c-MYC activity assay. Data show the mean absorbance ± SD (n = 3; ± SD). *Significantly different from controls (solvent only treated cells), determined by one-way ANOVA (**:P < 0.005, *:P < 0.05).

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Induction of apoptosis by NBT-272 in MB cells

The induction of apoptotic cell death upon treatment with NBT-272 was next assessed. In all MB cell lines tested, NBT-272 treatment (0.04 and 0.4 μg/ml for 24 h) induced apoptosis (Figure 5). Compared with untreated controls, the NBT-272 mediated increase in apoptosis was significant in DAOY wt (425% and 603%), DAOY V11 (443% and 613%), DAOY M2 (343% and 375%), D341 (147% and 162%), and in D425 human MB cells (124% and 178%). DAOY M2 cells were characterized by a higher basal apoptotic activity, when compared to DAOY wt and DAOY V11 cells. Interestingly, the apoptosis-inducing effects of NBT-272 were most prominent in MB cells displaying low c-MYC expression (DAOY wt, DAOY V11). In summary, apoptotic cell death does not appear to contribute equally to the reduction in viability in the MB cell lines under study.

Figure 5
figure 5

NBT-272 treatment (24 h) of human MB cells results in an increase of apoptotic cell death. Values represent the mean absorbance of cytoplasmatic histone-associated-DNA fragments (representative from two independent experiments) compared with solvent only treated cells ± SD (n = 3; ± SD). *Significantly different from control values, determined by one-way ANOVA (***:P < 0.001, **:P < 0.005, *:P < 0.05).

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Effects of NBT-272 on the cell cycle in MB cells

To determine possible effects of NBT-272 on cell cycle regulation, the cellular DNA content was assessed using flow cytometry. Under conditions where induction of apoptosis was observed (Figure 5), NBT-272 treatment (0.04 and 0.4 μg/ml for 24 h) resulted in no significant cell cycle alterations (data not shown). To examine whether MB cells are capable of transiting through the cell cycle in the presence of NBT-272, cultures were synchronized at the G1/S boundary by using hydroxyurea treatment. The fractions of MB cells in G1, S, and G2/M at different times (0, 8, 16, and 24 h after release) were analyzed (Figure 6). After the hydroxyurea block most of the cells were found to accumulate in the G1 phase (Figure 6). In each case, the untreated control cells transited through S into G2/M phase by 8–16 h (DAOY by 8 h, D341 by 16 h). In contrast, flow cytometric analysis of synchronized MB cells treated with 0.4 μg/ml NBT-272 demonstrated arrest in G1 or S.

Figure 6
figure 6

Block of the cell cycle progression in synchronized MB treated with NBT-272. MB cells were synchronized by hydroxyurea treatment as described in Methods and treated with NBT-272 or the same volume of medium as control. FACS analysis of NBT-272 and control cells carried out 0, 8, 16, and 24 h posttreatment. Results are presented as the mean percentages of cells in G1, S, and G2/M ± SD (n = 2; ± SD).

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Discussion

The present study shows for the first time that NBT-272 has anti-proliferative effects on human MB cell lines at nanomolar concentrations. DAOY cells with low c-MYC expression were slightly less sensitive to NBT-272 than D425 and D341 MB cells that are c-MYC amplified and express high levels of c-MYC. However, DAOY cells engineered to express increased levels of c-MYC showed only slightly enhanced sensitivity to NBT-272 when compared to parental DAOY cells. This indicates that the expression of c-MYC is not the only factor regulating the cellular sensitivity towards NBT-272. In an earlier study using leukemia and lymphoma cells, tumor cells expressing wild-type p53 and high c-MYC levels were most sensitive to bruceantin [22, 25]. The results of the current study are in accordance with these observations. The slightly more sensitive MB cell lines D341 and D425 express wild-type p53 and high c-MYC levels, whereas DAOY cells harbor mutant p53 [40].

Our results also demonstrate that NBT-272 induces apoptosis in MB cells at submicromolar concentrations. The induction of apoptosis by NBT-272 was more prominent in MB cells expressing low c-MYC levels (DAOY wt, DAOY V11), giving no indication that c-MYC down-regulation is related to the susceptibility of MB cells to NBT-272 induced apoptosis. D341 and D425 human MB cells do not express caspase-8 [37] and DAOY cells harbor mutant p53 [40]. Neither caspase-8, nor p53 appear to be essential for NBT-272 induced apoptosis. Induction of apoptosis by quassinoids has been reported before [25, 26, 41]. Rosati et al. [40] have demonstrated that quassinoids can induce mitochondrial depolarization and caspase-3 activation. The results of the current study are in accordance with these findings. However, further studies are needed to elucidate the exact mechanisms of NBT-272-induced apoptosis in MB cells.

NBT-272 treatment of synchronized MB cells resulted in a block in cell cycle progression in MB cells, which may explain its anti-proliferative effects. The data presented in this report support the existence of quassinoid mediated effects on the cell cycle regulation. Bruceantin treatment of leukemia and lymphoma cells induced a G1 arrest in most cell lines tested [22, 25]. It has been postulated that quassinoids inhibit c-MYC protein expression [25, 26], which is confirmed in the present study. The ability of c-MYC to activate and repress target genes involved in cell-cycle progression has been investigated intensively [42–44]. Down-regulation of c-MYC expression by antisense prevented S-phase entry [45, 46], indicating the important role of c-MYC in cell cycle progression. It has been suggested that quassinoids inhibit the purine synthesis pathway and inhibit DNA/RNA synthesis [47], and that inhibition of nucleotide synthesis may also contribute to the prolonged G1/S arrest. The exact mechanism by which NBT-272 inhibits cell cycle progression remains to be investigated.

The anti-proliferative effect of NBT-272 on MB cells was associated with c-MYC protein down-regulation and reduced c-MYC activity. The effect on c-MYC expression occurred at the post-transcriptional level, since the drug did not significantly affect c-MYC mRNA levels in MB cell lines. In MB, c-MYC gene amplification, aberrant signal transduction of the wingless (WNT) signalling pathway [48], or constitutive activation of STAT3 [49] are known mechanisms for c-MYC deregulation. In order to elucidate whether NBT-272 could influence these pathways, STAT3 activity was measured in cell lysates of cells treated with NBT-272. Our analysis revealed relatively low STAT3 activity in all MB cells and minor changes after NBT-272 treatment (data not shown). The NBT-272-mediated effect on c-MYC levels appears to be independent of STAT3 activity.

Conclusion

In human MB cells, NBT-272 treatment inhibits cellular proliferation at nanomolar concentrations, induces apoptosis, and down-regulates the expression of the oncogene c-MYC. Thus, NBT-272 represents a novel candidate drug to inhibit proliferation of human MB cells in vivo.

References

  1. Gurney JG, Smith MA, Bunin GR: CNS and miscellaneous intracranial and intraspinal neoplasms. SEER Pediatric Monograph. 2000, http://seer.cancer.gov/Publications/ , National Cancer Institute, 51-63.

    Google Scholar 

  2. Felsher DW, Bishop JM: Reversible tumorigenesis by MYC in hematopoietic lineages. Mol Cell. 1999, 4 (2): 199-207. 10.1016/S1097-2765(00)80367-6.

    Article  CAS  PubMed  Google Scholar 

  3. Shachaf CM, Kopelman AM, Arvanitis C, Karlsson A, Beer S, Mandl S, Bachmann MH, Borowsky AD, Ruebner B, Cardiff RD, Yang Q, Bishop JM, Contag CH, Felsher DW: MYC inactivation uncovers pluripotent differentiation and tumour dormancy in hepatocellular cancer. Nature. 2004, 431 (7012): 1112-1117. 10.1038/nature03043.

    Article  CAS  PubMed  Google Scholar 

  4. D'Cruz CM, Gunther EJ, Boxer RB, Hartman JL, Sintasath L, Moody SE, Cox JD, Ha SI, Belka GK, Golant A, Cardiff RD, Chodosh LA: c-MYC induces mammary tumorigenesis by means of a preferred pathway involving spontaneous Kras2 mutations. Nat Med. 2001, 7 (2): 235-239. 10.1038/84691.

    Article  PubMed  Google Scholar 

  5. Arnold I, Watt FM: c-Myc activation in transgenic mouse epidermis results in mobilization of stem cells and differentiation of their progeny. Curr Biol. 2001, 11 (8): 558-568. 10.1016/S0960-9822(01)00154-3.

    Article  CAS  PubMed  Google Scholar 

  6. Scheurlen WG, Schwabe GC, Joos S, Mollenhauer J, Sörensen N, Kühl J: Molecular analysis of childhood primitive neuroectodermal tumors defines markers associated with poor outcome. J Clin Oncol. 1998, 16: 2478-2485.

    CAS  PubMed  Google Scholar 

  7. Raffel C, Gilles FE, Weinberg KI: Reduction to homozyosity and gene amplification in central nervous system primitive neuroectodermal tumors of childhood. Cancer Res. 1990, 50: 587-591.

    CAS  PubMed  Google Scholar 

  8. Batra SK, McLendon RE, Koo JS, Castelino-Prabhu S, Fuchs HE, Krischer JP, Friedman HS, Bigner DD, Bigner SH: Prognostic implications of chromosome 17p deletions in human medulloblastoma. J Neurooncol. 1995, 24: 39-45. 10.1007/BF01052657.

    Article  CAS  PubMed  Google Scholar 

  9. Badiali M, Pession A, Basso G, Andreini L, Rigobello L, Galassi E, Giangaspero F: N-myc and c-myc oncogenes amplification in medulloblastomas. Evidence of particularly aggressive behavior of a tumor with c-myc amplification. Tumori. 1991, 77: 118-121.

    CAS  PubMed  Google Scholar 

  10. Wasson JC, Saylors III RL, Zeltzer P, Friedman HS, Bigner SH, Burger PC, Bigner DD, Look AT, Douglass EC, Brodeur GM: Oncogene amplification in pediatric brain tumors. Cancer Res. 1990, 50: 2987-2990.

    CAS  PubMed  Google Scholar 

  11. Herms J, Neidt I, Lüscher B, Sommer A, Schürmann P, Schröder T, Bergmann M, Wilken B, Probst-Cousin S, Hernaiz-Driever P, Behnke J, Hanefeld F, Pietsch T, Kretzschmar HA: C-myc expression in medulloblastoma and its prognsotic value. Int J Cancer. 2000, 89: 395-402. 10.1002/1097-0215(20000920)89:5<395::AID-IJC1>3.0.CO;2-V.

    Article  CAS  PubMed  Google Scholar 

  12. Grotzer MA, Hogarty MD, Janss AJ, Liu X, Zhao H, Eggert A, Sutton LN, Rorke LB, Brodeur GM, Phillips PC: MYC messenger RNA expression predicts survival outcome in childhood primitive neuroectodermal tumor/medulloblastoma. Clin Cancer Res. 2001, 7 (8): 2425-2433.

    CAS  PubMed  Google Scholar 

  13. Bruggers CS, Tai KF, Murdock T, Sivak L, Le K, Perkins SL, Coffin CM, Carroll WL: Expression of the c-Myc protein in childhood medulloblastoma. J Pediatr Hematol Oncol. 1998, 20 (1): 18-25. 10.1097/00043426-199801000-00003.

    Article  CAS  PubMed  Google Scholar 

  14. Bigner SH, Friedman HS, Vogelstein B, Oakes WJ, Bigner DD: Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts. Cancer Res. 1990, 50: 2347-2350.

    CAS  PubMed  Google Scholar 

  15. Aldosari N, Bigner SH, Burger PC, Becker L, Kepner JL, Friedman HS, McLendon RE: MYCC and MYCN oncogene amplification in medulloblastoma. A fluorescence in situ hybridization study on paraffin sections from the Children's Oncology Group. Arch Pathol Lab Med. 2002, 126: 540-545.

    PubMed  Google Scholar 

  16. Giangaspero F, Rigobello L, Badiali M, Loda M, Andreini L, Basso G, Zorzi F, Montaldi A: Large-cell medulloblastomas: a distinct variant with highly aggressive behavior. Am J Surg Pathol. 1992, 16: 687-693.

    Article  CAS  PubMed  Google Scholar 

  17. Eberhart CG, Kratz JE, Schuster A, Goldthwaite P, Cohen KJ, Perlman EJ, Burger PC: Comparative genomic hybridization detects an increased number of chromosomal alterations in large cell/anaplastic medulloblastomas. Brain Pathol. 2002, 12 (1): 36-44.

    Article  CAS  PubMed  Google Scholar 

  18. Jay V, Squire J, Bayani J, Alkhani AM, Rutka JT, Zielenska M: Oncogene amplification in medulloblastoma: analysis of a case by comparative genomic hybridization and fluorescence in situ hybridization. Pathology. 1999, 31: 337-344. 10.1080/003130299104693.

    Article  CAS  PubMed  Google Scholar 

  19. Eberhart CG, Kratz J, Wang Y, Summers K, Stearns D, Cohen K, Dang CV, Burger PC: Histopathological and molecular prognostic markers in medulloblastoma: c-myc, N-myc, TrkC, and anaplasia. J Neuropathol Exp Neurol. 2004, 63 (5): 441-449.

    Article  CAS  PubMed  Google Scholar 

  20. Stearns D, Chaudhry A, Abel TW, Burger PC, Dang CV, Eberhart CG: c-myc overexpression causes anaplasia in medulloblastoma. Cancer Res. 2006, 66 (2): 673-681. 10.1158/0008-5472.CAN-05-1580.

    Article  CAS  PubMed  Google Scholar 

  21. Fukamiya N, Lee KH, Muhammad I, Murakami C, Okano M, Harvey I, Pelletier J: Structure-activity relationships of quassinoids for eukaryotic protein synthesis. Cancer Lett. 2005, 220 (1): 37-48. 10.1016/j.canlet.2004.04.023.

    Article  CAS  PubMed  Google Scholar 

  22. Cuendet M, Pezzuto JM: Antitumor activity of bruceantin: an old drug with new promise. J Nat Prod. 2004, 67 (2): 269-272. 10.1021/np030304+.

    Article  CAS  PubMed  Google Scholar 

  23. Liao LL, Kupchan SM, Horwitz SB: Mode of action of the antitumor compound bruceantin, an inhibitor of protein synthesis. Mol Pharmacol. 1976, 12 (1): 167-176.

    CAS  PubMed  Google Scholar 

  24. Fresno M, Carrasco L, Vazquez D: Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation. Eur J Biochem. 1976, 68 (2): 355-364. 10.1111/j.1432-1033.1976.tb10822.x.

    Article  CAS  PubMed  Google Scholar 

  25. Mata-Greenwood E, Cuendet M, Sher D, Gustin D, Stock W, Pezzuto JM: Brusatol-mediated induction of leukemic cell differentiation and G(1) arrest is associated with down-regulation of c-myc. Leukemia. 2002, 16 (11): 2275-2284. 10.1038/sj.leu.2402696.

    Article  CAS  PubMed  Google Scholar 

  26. Cuendet M, Christov K, Lantvit DD, Deng Y, Hedayat S, Helson L, McChesney JD, Pezzuto JM: Multiple myeloma regression mediated by bruceantin. Clin Cancer Res. 2004, 10 (3): 1170-1179. 10.1158/1078-0432.CCR-0362-3.

    Article  CAS  PubMed  Google Scholar 

  27. Liesmann J, Belt RJ, Haas CD, Hoogstraten B: Phase I study on bruceantin administered on a weekly schedule. Cancer Treat Rep. 1981, 65 (9-10): 883-885.

    CAS  PubMed  Google Scholar 

  28. Bedikian AY, Valdivieso M, Bodey GP, Murphy WK, Freireich EJ: Initial clinical studies with bruceantin. Cancer Treat Rep. 1979, 63 (11-12): 1843-1847.

    CAS  PubMed  Google Scholar 

  29. Garnick MB, Blum RH, Canellos GP, Mayer RJ, Parker L, Skarin AT, Li FP, Henderson IC, Frei E: Phase I trial of bruceantin. Cancer Treat Rep. 1979, 63 (11-12): 1929-1932.

    CAS  PubMed  Google Scholar 

  30. Wiseman CL, Yap HY, Bedikian AY, Bodey GP, Blumenschein GR: Phase II trial of bruceantin in metastatic breast carcinoma. Am J Clin Oncol. 1982, 5 (4): 389-391.

    Article  CAS  PubMed  Google Scholar 

  31. Arseneau JC, Wolter JM, Kuperminc M, Ruckdeschel JC: A Phase II study of Bruceantin (NSC-165, 563) in advanced malignant melanoma. Invest New Drugs. 1983, 1 (3): 239-242. 10.1007/BF00208896.

    Article  CAS  PubMed  Google Scholar 

  32. Helson L MCJ: In vitro cytotoxic activity of NBT-272, a novel quassinoid analog. Eur J Cancer Suppl. 2004, Geneva , 2 (8): 171-10.1016/S1359-6349(04)80572-X.

  33. Zuzak TJ, Steinhoff DF, Sutton LN, Phillips PC, Eggert A, Grotzer MA: Loss of caspase-8 mRNA expression is common in childhood primitive neuroectodermal brain tumour/medulloblastoma. Eur J Cancer. 2002, 38 (1): 83-91. 10.1016/S0959-8049(01)00355-0.

    Article  CAS  PubMed  Google Scholar 

  34. Bieche I, Nogues C, Paradis V, Olivi M, Bedossa P, Lidereau R, Vidaud M: Quantitation of hTERT gene expression in sporadic breast tumors with a real-time reverse transcription-polymerase chain reaction assay. Clin Cancer Res. 2000, 6 (2): 452-459.

    CAS  PubMed  Google Scholar 

  35. Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R, Mathieu C: An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods. 2001, 25 (4): 386-401. 10.1006/meth.2001.1261.

    Article  CAS  PubMed  Google Scholar 

  36. Sharma-Walia N, Krishnan HH, Naranatt PP, Zeng L, Smith MS, Chandran B: ERK1/2 and MEK1/2 induced by Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) early during infection of target cells are essential for expression of viral genes and for establishment of infection. J Virol. 2005, 79 (16): 10308-10329. 10.1128/JVI.79.16.10308-10329.2005.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Grotzer MA, Eggert A, Zuzak TJ, Janss AJ, Marwaha S, Wiewrodt BR, Ikegaki N, Brodeur GM, Phillips PC: Resistance to TRAIL-induced apoptosis in primitive neuroectodermal brain tumor cells correlates with a loss of caspase-8 expression. Oncogene. 2000, 19 (40): 4604-4610. 10.1038/sj.onc.1203816.

    Article  CAS  PubMed  Google Scholar 

  38. Buchkovich K, Duffy LA, Harlow E: The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. Cell. 1989, 58 (6): 1097-1105. 10.1016/0092-8674(89)90508-4.

    Article  CAS  PubMed  Google Scholar 

  39. Stojic L, Mojas N, Cejka P, Di Pietro M, Ferrari S, Marra G, Jiricny J: Mismatch repair-dependent G2 checkpoint induced by low doses of SN1 type methylating agents requires the ATR kinase. Genes Dev. 2004, 18 (11): 1331-1344. 10.1101/gad.294404.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Saylors III RL, Sidransky D, Friedman HS, Bigner SH, Bigner DD, Vogelstein B, Brodeur GM: Infrequent p53 gene mutations in medulloblastomas. Cancer Res. 1991, 51: 4721-4723.

    Google Scholar 

  41. Rosati A, Quaranta E, Ammirante M, Turco MC, Leone A, De Feo V: Quassinoids can induce mitochondrial membrane depolarisation and caspase 3 activation in human cells. Cell Death Differ. 2004, 11 Suppl 2: S216-8. 10.1038/sj.cdd.4401534.

    Article  CAS  PubMed  Google Scholar 

  42. Pelengaris S, Khan M, Evan G: c-MYC: more than just a matter of life and death. Nat Rev Cancer. 2002, 2 (10): 764-776. 10.1038/nrc904.

    Article  CAS  PubMed  Google Scholar 

  43. Obaya AJ, Mateyak MK, Sedivy JM: Mysterious liaisons: the relationship between c-Myc and the cell cycle. Oncogene. 1999, 18 (19): 2934-2941. 10.1038/sj.onc.1202749.

    Article  CAS  PubMed  Google Scholar 

  44. Prendergast GC: Mechanisms of apoptosis by c-Myc. Oncogene. 1999, 18 (19): 2967-2987. 10.1038/sj.onc.1202727.

    Article  CAS  PubMed  Google Scholar 

  45. Heikkila R, Schwab G, Wickstrom E, Loke SL, Pluznik DH, Watt R, Neckers LM: A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but not progress from G0 to G1. Nature. 1987, 328 (6129): 445-449. 10.1038/328445a0.

    Article  CAS  PubMed  Google Scholar 

  46. Holt JT, Redner RL, Nienhuis AW: An oligomer complementary to c-myc mRNA inhibits proliferation of HL-60 promyelocytic cells and induces differentiation. Mol Cell Biol. 1988, 8 (2): 963-973.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Hall IH, Lee KH, Eigebaly SA, Imakura Y, Sumida Y, Wu RY: Antitumor agents. XXXIV: Mechanism of action of bruceoside A and brusatol on nucleic acid metabolism of P-388 lymphocytic leukemia cells. J Pharm Sci. 1979, 68 (7): 883-887. 10.1002/jps.2600680726.

    Article  CAS  PubMed  Google Scholar 

  48. Gilbertson RJ: Medulloblastoma: signalling a change in treatment. Lancet Oncol. 2004, 5 (4): 209-218. 10.1016/S1470-2045(04)01424-X.

    Article  PubMed  Google Scholar 

  49. Schaefer LK, Ren Z, Fuller GN, Schaefer TS: Constitutive activation of Stat3alpha in brain tumors: localization to tumor endothelial cells and activation by the endothelial tyrosine kinase receptor (VEGFR-2). Oncogene. 2002, 21 (13): 2058-2065. 10.1038/sj.onc.1205263.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was financially supported by the Swiss National Fonds and the Swiss Research Foundation Child and Cancer. We thank Professor Dr Josef Jiricny from the University of Zurich, and Dr Claudio Vallan from the Flow-Cytometry and Cell Sorting Laboratory of the Institute of Clinical Research, University of Berne for advice; and Eva Niederer of the Flow-Cytometry Laboratory of the Institute of Biomedical Engineering of the Swiss Federal Institute of Technology for technical support.

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Authors and Affiliations

  1. Neuro-Oncology Program, University Children's Hospital, Zurich, Switzerland

    André O von Bueren, Tarek Shalaby, Julia Rajtarova & Michael A Grotzer

  2. Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA

    Duncan Stearns & Charles G Eberhart

  3. TAPESTRY Pharmaceuticals, Boulder, Colorado, USA

    Lawrence Helson

  4. Division of Clinical Chemistry and Biochemistry, University Children's Hospital, Zurich, Switzerland

    Alexandre Arcaro

Authors
  1. André O von Bueren
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  2. Tarek Shalaby
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  6. Lawrence Helson
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  8. Michael A Grotzer
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Corresponding author

Correspondence to Michael A Grotzer.

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Competing interests

Dr. Lawrence Helson is an employee of TAPESTRY Pharmaceuticals. TAPESTRY Pharmaceuticals is the manufacturer of this compound and provided the NBT-272 employed in the study.

Authors' contributions

AOVB performed most experimental work including data analysis and he drafted the manuscript. TS participated in the study design and supervised the experimental work. JR carried out most of the RT-PCR analysis. DS and CGE stably transfected the DAOY cell lines and participated in study design and data analysis. LH provided the drug and participated in the study design. MAG and AA lead the study and finalized the manuscript. All authors read and approved the final manuscript.

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von Bueren, A.O., Shalaby, T., Rajtarova, J. et al. Anti-proliferative activity of the quassinoid NBT-272 in childhood medulloblastoma cells. BMC Cancer 7, 19 (2007). https://doi.org/10.1186/1471-2407-7-19

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  • DOI: https://doi.org/10.1186/1471-2407-7-19

Keywords

  • Medulloblastoma
  • DAOY Cell
  • Numerous Human Cancer
  • Consensus Binding Site Sequence
  • Bruceantin

BMC Cancer

ISSN: 1471-2407

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