- Research article
- Open Access
- Open Peer Review
This article has Open Peer Review reports available.
E7080, a multi-targeted tyrosine kinase inhibitor suppresses tumor cell migration and invasion
© Glen et al; licensee BioMed Central Ltd. 2011
Received: 14 April 2011
Accepted: 22 July 2011
Published: 22 July 2011
E7080 is an orally active multi-targeted kinase inhibitor whose targets include vascular endothelial growth factor receptors (VEGFR), fibroblast growth factor receptor (FGFR) and platelet derived growth factor receptors (PDGFR). It has been shown to inhibit tumor angiogenesis by targeting endothelial cells. A number of the targets of E7080 are also expressed on tumor cells and here we have looked at the direct effects of E7080 on tumor cell behavior.
Using a panel of human tumor cell lines we determined the effect of E7080 on cell proliferation, migration and invasion. Inhibition of FGFR and PDGFR signaling in the cells was measured.
E7080 had little effect on tumor cell proliferation. However, it blocked migration and invasion at concentrations that inhibited FGFR and PDGFR signaling. Knock-down of PDGFR-β in U2OS osteosarcoma cells also inhibited cell migration which, could not be further inhibited in the presence of E7080. Furthermore, E7080 could not inhibit the migration of a PDGFR negative cell line.
E7080 does not significantly affect tumor cell proliferation but can inhibit their migration and invasion at concentrations that both inhibit its known targets and are achievable clinically.
Angiogenesis, the formation of new blood vessels, is required for tumor growth and metastasis [1, 2]. The ability of tumors to promote angiogenesis is driven by expression of pro-angiogenic factors such as VEGF, bFGF, PDGF and transforming growth factor-β (TGF-β) of which VEGF is the most important [1, 3]. VEGF exerts its actions through two receptor tyrosine kinases, VEGFR-1 and VEGFR-2, but it is signaling via VEGFR-2 that is important for tumor angiogenesis . A third VEGF receptor VEGFR-3, is important for lymphangiogenesis and is activated by two different ligands, VEGF-C and VEGF-D . In view of the dependence of tumors on angiogenesis for sustained growth, targeting angiogenesis has been the focus of much research into new anti-cancer therapies in recent years . Direct angiogenesis inhibitors target endothelial cells by inhibiting their ability to proliferate, migrate and form new blood vessels. The first example of this was the use of an antibody to VEGF, which was able to inhibit the growth of tumors in mouse models . Subsequently a humanized version of this antibody, bevacizumab, was developed which showed promising evidence of efficacy in pre-clinical models. However, this was not translated into the clinical setting. With the exception of renal carcinoma and glioblastoma multiforme [8, 9], bevacizumab appears to have little activity as a single agent, although it does confer significant benefit when combined with cytotoxic agents and is now approved by the US Food and Drug Administration for use in colorectal, breast, lung and renal cancer, and glioblastoma multiforme. Other approaches to target angiogenesis have centered on the use of small molecule kinase inhibitors, which target VEGFR-2. Many of these inhibitors are multi-targeted with additional activity against one or a number of other receptor tyrosine kinases. One such inhibitor is E7080, an orally active multi-targeted tyrosine kinase inhibitor, which is currently in clinical development. Three dose escalation phase I trials of E7080 have now been performed, examining different dosing schedules [10–12]. In view of promising anti-tumor effects observed, a number of disease specific phase II and III trials, including melanoma, renal, thyroid, ovarian, hepatocellular and endometrial cancer, are now underway. E7080 is a potent inhibitor of VEGFR-2 and VEGFR-3 with IC50s of 4 and 5.2 nM respectively, but also has activity against VEGFR-1, FGFR-1, and PDGFRα/β tyrosine kinases although the IC50s are around 10 fold higher . E7080 shows anti-tumor activity in human cancer cell xenografts, which has been attributed to its ability to inhibit angiogenesis predominantly through effects on VEGFR-2 inhibition but also through inhibition of KIT and FGFR-1 [13–15]. Inhibition of VEGFR-3 mediated lymphangiogenesis by E7080 also contributed to its ability to suppress lymph node and lung metastases in a mammary tumor model . Although these effects were not mediated via direct effects of E7080 on tumor cells two of the main targets of E7080, FGFR-1 and PDFGR, are expressed in a number of solid tumors. We therefore set out to determine whether E7080, in addition to effects on angiogenesis, could directly affect the behavior of epithelial tumor cells.
FGFR-1 amplification and over expression has been reported in a sub-set of breast cancers , lung squamous cell carcinoma  and also in oral squamous carcinoma, rhabodomyosarcoma, ovarian and bladder cancer . In melanoma there is evidence for a bFGF/FGFR-1 autocrine loop driving proliferation in vivo [20, 21] indicating that in some tumor types inhibitors such as E7080 may also have direct effects on the proliferation of tumor cells. The PDGFR and its ligands are also expressed in a number of different tumor types including gliomas, breast and ovarian cancer and there is evidence for PDGFR autocrine growth control in gliomas [22, 23]. However, PDGFR signaling is also important in tumor stromal cells and many effects of PDGF in tumor cells may be mediated via paracrine activation of stromal cells [24, 25] and in particular endothelial cells [23, 26]. The effects of E7080 on tumor angiogenesis may therefore in part be mediated via inhibition of PDGFR signaling in endothelial cells. A number of small molecule tyrosine kinase inhibitors that target both the FGFR and the PDGFR are now in clinical development, although, like E7080, they also inhibit a number of other receptor tyrosine kinases making it difficult to determine the importance of targeting each individual receptor kinase to tumor development [19, 22, 27].
Cancer cell proliferation is often regarded as the most important aspect of cancer progression, and traditionally, anti-cancer treatment has focused on preventing growth of the tumor. However, other key aspects play a crucial role in cancer progression, including tissue invasion and metastasis . A malignant tumor's ability to invade and migrate differentiates it from a benign tumor, and hence these processes are crucial for cancer progression. 90% of human cancer deaths are caused by distant metastases and therefore preventing cancer spread could have a huge impact on patient survival. Signaling via both the FGFR-1 and PDGFR has been associated with increased migration, invasion and metastatic potential [29–33].
We therefore set out to determine whether E7080 has any direct effects on tumor cell proliferation, migration and invasion. We show that although there was very little effect of E7080 on the proliferation of a range of human tumor cell lines both their migration and invasion could be blocked at concentrations of E7080 that inhibited signaling through FGFR-1 and PDGFR-β. Furthermore, we show that U2OS osteosarcoma cell migration was inhibited by both E7080 and knock-down of PDGFR-β expression suggesting that E7080 may directly affect the migratory capacity of tumor cells by targeting PDGFR-β. The ability of E7080 to inhibit both migration and invasion in vitro implies that it could potentially have significant benefit in the prevention (or at least in the reduction) of development of tumor metastases in vivo.
Cell culture and reagents
A375, U2OS, DU145 and human umbilical vein endothelial cells (HUVECs) were obtained from the American Type Culture Collection (Manassas, VA, USA). KM12C cells were from I Fidler (MD Anderson Cancer Centre, Houston, TX, USA) and DX3 and SK23 cells were a kind gift from B Ozanne (Beatson Institute, Glasgow, UK). DU145 and KM12C cells were grown in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and 2 mM glutamine and MEM supplemented with 10% FBS and 2 mM glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids respectively. HUVECs were grown in media 199 supplemented with 20% FBS, heparin, 1% penicillin/streptomycin/fungizone and endothelial cell growth supplement. All other cell lines were grown in DMEM supplemented with 10% FBS and 2 mM glutamine. FBS was obtained from Autogen Bioclear (Calne, UK) and all other tissue culture reagents from Invitrogen (Paisley, UK). All lines were maintained in a humidified atmosphere of 5% CO2 at 37°C and were serum starved prior to growth factor stimulation. Growth factor treatments were: bFGF (100 ng/ml, 15 min), PDGF-BB (50 ng/ml, 15 min) and VEGF-A (100 ng/ml, 15 min)(all Upstate Biotechnology, Waltham, MA, USA). E7080 (Eisai Co. Ltd., Ibaraki, Japan) was prepared as a 10 mM stock in DMSO and diluted in culture media prior to use. For PDGFR-β knock-down experiments cells were transfected with either 50 nM of ON-TARGETplus SMARTpool PDGFR-β or non-targeting siRNA oligonucleotides according to the manufacturers guidelines (Dharmacon, Thermo Scientific, Loughborough, UK). 72 hours after transfection cell extracts were prepared or cells were plated out for wound healing assays as described below.
Cell proliferation assay
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) proliferation assays were carried out as described previously . Cells were treated with a range of E7080 concentrations for 72 hours. Curve fitting and generation of IC50 values was carried out using GraphPad Prism 4 software from quadruplicate wells.
Immunoprecipitation and immunoblot analysis
Cell extracts were prepared as described previously  and then immunoprecipitated with PDGFR-β (Upstate Biotechnology), FRS2 (Santa Cruz, Santa Cruz, CA, USA) or VEGFR-2 (Cell Signaling, Danvers, MA, USA) antibodies overnight. Immune complexes were collected with protein-A or protein-G sepharose beads (Sigma, Gillingham, UK) and immunoblot analysis then carried out as described previously  using the above antibodies and also an anti-phosphotyrosine antibody (PY20, BD Transduction Laboratories, Oxford, UK).
Cells were plated in 6 well plates at low cell density. After 24 hours E7080 was added and images captured every 5 minutes for 16 hours on a Nikon TE2000 microscope using a 10× objective. Image J software was used to track the cell nuclei of 20 separate cells per condition over time and calculate the total accumulated distance, euclidean distance (straight line distance travelled) and velocity. Wound healing assays were carried out on confluent monolayers of cells after 'wounds' were made by scoring the monolayer with a fine pipette tip. E7080 was then added and images captured every 15 minutes for 16 hours on a Nikon TE2000 microscope using a 10× objective. Wound closure was then quantified using Image J by calculating the distance between the wound edges from 8 images per treatment condition.
Inverse invasion assays were performed as described previously using FBS as a chemoattractant . In brief, cells were seeded on the bottom of Transwell inserts (Corning, Fisher Scientific, Loughborough, UK) containing polymerized collagen type I (Becton Dickinson, Oxford, UK). Transwell inserts were then placed in serum-free medium and medium supplemented with 10% FBS was placed on top of the matrix in the presence or absence of E7080. Five days after seeding invading cells were stained with Calcein AM (Invitrogen) and visualized using a Leica SP2 confocal microscope. Serial optical sections were captured at 15 μm intervals and quantified using ImageJ analysis software. Invasion was calculated as cells that had moved more than 20 μm into the collagen.
Effects of E7080 on tumor cell proliferation
IC50 values for E7080 in a panel of cell lines
95% Confidence interval
37.24 - 51.09
20.02 - 27.20
21.11 - 46.79
7.94 - 11.14
40.19 - 44.94
31.36 - 38.28
Inhibition of FGFR-1 and PDGFR-β signaling by E7080
E7080 inhibits cell migration and invasion
PDGFR-β signaling is required for U2OS cell migration
E7080 is a promising new multi-targeted kinase inhibitor that is currently in clinical development. The main targets of E7080 are VEGFR-2 and VEGFR-3 and the preclinical work to date has focused on the anti-angiogenic activity of E7080. However, in this study we show that E7080 also has direct effects on tumor cells at concentrations that correlate with inhibition of other known targets, notably FGFR-1 and PDGFR-β. E7080 was able to inhibit both tumor cell migration and invasion but had little effect on tumor cell proliferation. As with many molecularly targeted agents there still remain questions about the clinical utility of E7080 in terms of which patients will benefit and in which setting treatment with E7080 would be most appropriate. A detailed preclinical investigation of the mechanism of action of drugs such as E7080, which have multiple kinase targets, is therefore essential to maximize its potential clinical application. FGFR-1 and PDGFR-β are both widely expressed in epithelial tumors and there is growing interest in both of these kinases as potential targets although many of the inhibitors currently available, as with E7080, also inhibit a number of other kinases [19, 22, 27]. It is likely therefore that such inhibitors will have multiple effects on both tumor cells and associated endothelial and stromal cells.
The pharmacokinetic analyses of patient samples from the phase I clinical trial of E7080 indicated that patients on the higher dose cohorts (receiving doses that will be recommended for further clinical development) typically achieved peak plasma concentrations of greater than 1 μM (manuscript submitted). This would suggest that inhibition of FGFR and PDGFR-β should be occurring in patients receiving treatment doses of E7080 and is greatly in excess of the concentrations required to inhibit VEGFR-2 activity (Figure 2). Inhibition of tumor cell proliferation was only seen at very high concentrations of E7080. These were in excess both of those achieved clinically and those required to inhibit the main known targets of E7080. This is in agreement with previous studies, which have shown minimal effects of E7080 on tumor cell growth in vitro . However, the situation in vivo may be very different as the tumor micro-environment often plays an important part in tumor growth. For example, there is evidence that melanoma is driven in part by bFGF acting on FGFR-1 on tumor cells. bFGF is released from tumor cells, endothelial cells and surrounding stromal cells, and therefore acts in an autocrine and paracrine fashion. This may lead to high local concentrations of bFGF, which may then be important for driving tumor cell proliferation. In addition over-expression of receptors or activating mutations may mean that some tumor cells are more dependent on these kinases for proliferation.
Although the evidence for direct effects of E7080 on tumor proliferation are not compelling we did see significant effects of E7080 on tumor cell migration and invasion at concentrations that both inhibit its known targets and are achievable clinically. Our data suggests that these effects of E7080 may be due to inhibition of PDGFR signaling. PDGFR signaling has been known for many years to regulate cell migration [36, 39] via activation of a number of signaling pathways including small Rho GTPases, phosphatidylinositol (PI)3-kinase and phospholipase (PL)C-γ  and in tumor cells this has been linked to activation of PI3-kinase [29, 40]. PDGF signaling also regulates the invasion of tumor cells [29, 31, 41]. In pancreatic cancer this is associated with PDGF induced phosphorylation of MUC1 a transmembrane glycoprotein involved in pancreatic tumor progression and metastasis . PDGFR signaling has been implicated in the development of metastases in a variety of human tumors, including oesophageal cancer , colorectal cancer [24, 25], pancreatic cancer , sarcoma  and non-small cell lung cancer . In addition in a mouse model of mammary carcinogenesis autocrine PDGFR signaling promotes metastasis through regulation of an epithelial-to-mesenchymal transition (EMT) and treatment with the PDGFR inhibitor imatinib suppressed metastasis .
In addition to our reported effects of E7080 on cell migration and invasion, E7080 significantly reduced lymphatic vessel density in MDA-MB-231 mammary tumor xenografts, which corresponded to a complete inhibition of both lymph node metastasis and lung metastasis in E7080-treated mice, thought to be due to the effects of E7080 on VEGFR-3 activity . Thus in addition to effects on angiogenesis E7080 may have additional effects on the development of metastases. Anti-invasive strategies are increasingly being investigated in patients with cancer. However, the clinical development of anti-invasive therapies is often hampered by their lack of direct anti-tumour effects and the difficulties involved with trial design and as yet it is not clear how they will be used in the clinical setting.
E7080 does not significantly affect tumor cell proliferation but can inhibit their migration and invasion. Direct effects of multi-kinase inhibitors such as E7080 on tumor cells should be considered as they enter the clinic and based on these findings, and given the knowledge we have gained regarding the favorable efficacy and tolerability of E7080 (even after long term use), consideration should be made to the potential effects of E7080 on the development of metastatic disease.
This work was supported by Eisai Co Ltd. and Cancer Research UK program grant C157/A9148.
- Carmeliet P, Jain RK: Angiogenesis in cancer and other diseases. Nature. 2000, 407 (6801): 249-257. 10.1038/35025220.View ArticlePubMedGoogle Scholar
- Hanahan D, Folkman J: Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell. 1996, 86 (3): 353-364. 10.1016/S0092-8674(00)80108-7.View ArticlePubMedGoogle Scholar
- Dvorak HF: Vascular permeability factor/vascular endothelial growth factor: a critical cytokine in tumor angiogenesis and a potential target for diagnosis and therapy. J Clin Oncol. 2002, 20 (21): 4368-4380. 10.1200/JCO.2002.10.088.View ArticlePubMedGoogle Scholar
- Shalaby F, Rossant J, Yamaguchi TP, Gertsenstein M, Wu XF, Breitman ML, Schuh AC: Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature. 1995, 376 (6535): 62-66. 10.1038/376062a0.View ArticlePubMedGoogle Scholar
- Karkkainen MJ, Makinen T, Alitalo K: Lymphatic endothelium: a new frontier of metastasis research. Nat Cell Biol. 2002, 4 (1): E2-5. 10.1038/ncb0102-e2.View ArticlePubMedGoogle Scholar
- Kerbel R, Folkman J: Clinical translation of angiogenesis inhibitors. Nat Rev Cancer. 2002, 2 (10): 727-739. 10.1038/nrc905.View ArticlePubMedGoogle Scholar
- Paz K, Zhu Z: Development of angiogenesis inhibitors to vascular endothelial growth factor receptor 2. Current status and future perspective. Front Biosci. 2005, 10: 1415-1439. 10.2741/1629.View ArticlePubMedGoogle Scholar
- Kreisl TN, Kim L, Moore K, Duic P, Royce C, Stroud I, Garren N, Mackey M, Butman JA, Camphausen K, et al: Phase II trial of single-agent bevacizumab followed by bevacizumab plus irinotecan at tumor progression in recurrent glioblastoma. J Clin Oncol. 2009, 27 (5): 740-745. 10.1200/JCO.2008.16.3055.View ArticlePubMedGoogle Scholar
- Yang JC, Haworth L, Sherry RM, Hwu P, Schwartzentruber DJ, Topalian SL, Steinberg SM, Chen HX, Rosenberg SA: A randomized trial of bevacizumab, an anti-vascular endothelial growth factor antibody, for metastatic renal cancer. N Engl J Med. 2003, 349 (5): 427-434. 10.1056/NEJMoa021491.View ArticlePubMedPubMed CentralGoogle Scholar
- Glen H, Boss DR, Morrison R, Roelvink M, Wanders J, Mazur A, Gupta A, Das A, Evans TR, Schellens JH: A phase I study of E7080 in patients (pts) with advanced malignancies. J Clin Oncol (Meeting Abstracts). 2008, 26 (15_suppl): 3526-Google Scholar
- Nemunaitis JJ, Senzer NN, Kurzrock R, Ng CS, Das A, Atienza RS, Zang EA, Jansen M, Ashworth S, Hong DS: Phase I dose-escalation study of E7080, a multikinase inhibitor, in patients with advanced solid tumors. J Clin Oncol (Meeting Abstracts). 2008, 26 (15_suppl): 14583-Google Scholar
- Yamada K, Hirata T, Fujiwara Y, Nokihara H, Yamamoto N, Yamada Y, Koizumi F, Nishio K, Koyama N, Tamura T: Phase I dose escalation study and biomarker analysis of E7080 in patients with advanced solid tumors. J Clin Oncol (Meeting Abstracts). 2008, 26 (15_suppl): 3527-Google Scholar
- Matsui J, Yamamoto Y, Funahashi Y, Tsuruoka A, Watanabe T, Wakabayashi T, Uenaka T, Asada M: E7080, a novel inhibitor that targets multiple kinases, has potent antitumor activities against stem cell factor producing human small cell lung cancer H146, based on angiogenesis inhibition. Int J Cancer. 2008, 122 (3): 664-671. 10.1002/ijc.23131.View ArticlePubMedGoogle Scholar
- Bruheim S, Kristian A, Uenaka T, Suo Z, Tsuruoka A, Nesland JM, Fodstad O: Antitumour activity of oral E7080, a novel inhibitor of multiple tyrosine kinases, in human sarcoma xenografts. Int J Cancer. 2011Google Scholar
- Ikuta K, Yano S, Trung VT, Hanibuchi M, Goto H, Li Q, Wang W, Yamada T, Ogino H, Kakiuchi S, et al: E7080, a multi-tyrosine kinase inhibitor, suppresses the progression of malignant pleural mesothelioma with different proangiogenic cytokine production profiles. Clin Cancer Res. 2009, 15 (23): 7229-7237. 10.1158/1078-0432.CCR-09-1980.View ArticlePubMedGoogle Scholar
- Matsui J, Funahashi Y, Uenaka T, Watanabe T, Tsuruoka A, Asada M: Multi-kinase inhibitor E7080 suppresses lymph node and lung metastases of human mammary breast tumor MDA-MB-231 via inhibition of vascular endothelial growth factor-receptor (VEGF-R) 2 and VEGF-R3 kinase. Clin Cancer Res. 2008, 14 (17): 5459-5465. 10.1158/1078-0432.CCR-07-5270.View ArticlePubMedGoogle Scholar
- Hynes NE, Dey JH: Potential for targeting the fibroblast growth factor receptors in breast cancer. Cancer Res. 2010, 70 (13): 5199-5202. 10.1158/0008-5472.CAN-10-0918.View ArticlePubMedGoogle Scholar
- Weiss J, Sos ML, Seidel D, Peifer M, Zander T, Heuckmann JM, Ullrich RT, Menon R, Maier S, Soltermann A, et al: Frequent and focal FGFR1 amplification associates with therapeutically tractable FGFR1 dependency in squamous cell lung cancer. Science translational medicine. 2010, 2 (62): 62ra93-10.1126/scitranslmed.3001451.View ArticlePubMedPubMed CentralGoogle Scholar
- Turner N, Grose R: Fibroblast growth factor signalling: from development to cancer. Nat Rev Cancer. 2010, 10 (2): 116-129. 10.1038/nrc2780.View ArticlePubMedGoogle Scholar
- Valesky M, Spang AJ, Fisher GW, Farkas DL, Becker D: Noninvasive dynamic fluorescence imaging of human melanomas reveals that targeted inhibition of bFGF or FGFR-1 in melanoma cells blocks tumor growth by apoptosis. Mol Med. 2002, 8 (2): 103-112.PubMedPubMed CentralGoogle Scholar
- Wang Y, Becker D: Antisense targeting of basic fibroblast growth factor and fibroblast growth factor receptor-1 in human melanomas blocks intratumoral angiogenesis and tumor growth. Nat Med. 1997, 3 (8): 887-893. 10.1038/nm0897-887.View ArticlePubMedGoogle Scholar
- Board R, Jayson GC: Platelet-derived growth factor receptor (PDGFR): a target for anticancer therapeutics. Drug Resist Updat. 2005, 8 (1-2): 75-83. 10.1016/j.drup.2005.03.004.View ArticlePubMedGoogle Scholar
- Pietras K, Sjoblom T, Rubin K, Heldin CH, Ostman A: PDGF receptors as cancer drug targets. Cancer Cell. 2003, 3 (5): 439-443. 10.1016/S1535-6108(03)00089-8.View ArticlePubMedGoogle Scholar
- Kitadai Y, Sasaki T, Kuwai T, Nakamura T, Bucana CD, Fidler IJ: Targeting the expression of platelet-derived growth factor receptor by reactive stroma inhibits growth and metastasis of human colon carcinoma. Am J Pathol. 2006, 169 (6): 2054-2065. 10.2353/ajpath.2006.060653.View ArticlePubMedPubMed CentralGoogle Scholar
- Kitadai Y, Sasaki T, Kuwai T, Nakamura T, Bucana CD, Hamilton SR, Fidler IJ: Expression of activated platelet-derived growth factor receptor in stromal cells of human colon carcinomas is associated with metastatic potential. Int J Cancer. 2006, 119 (11): 2567-2574. 10.1002/ijc.22229.View ArticlePubMedGoogle Scholar
- Uehara H, Kim SJ, Karashima T, Shepherd DL, Fan D, Tsan R, Killion JJ, Logothetis C, Mathew P, Fidler IJ: Effects of blocking platelet-derived growth factor-receptor signaling in a mouse model of experimental prostate cancer bone metastases. J Natl Cancer Inst. 2003, 95 (6): 458-470. 10.1093/jnci/95.6.458.View ArticlePubMedGoogle Scholar
- Knights V, Cook SJ: De-regulated FGF receptors as therapeutic targets in cancer. Pharmacol Ther. 2010, 125 (1): 105-117. 10.1016/j.pharmthera.2009.10.001.View ArticlePubMedGoogle Scholar
- Hanahan D, Weinberg RA: The hallmarks of cancer. Cell. 2000, 100 (1): 57-70. 10.1016/S0092-8674(00)81683-9.View ArticlePubMedGoogle Scholar
- Abouantoun TJ, Macdonald TJ: Imatinib blocks migration and invasion of medulloblastoma cells by concurrently inhibiting activation of platelet-derived growth factor receptor and transactivation of epidermal growth factor receptor. Mol Cancer Ther. 2009Google Scholar
- Jechlinger M, Sommer A, Moriggl R, Seither P, Kraut N, Capodiecci P, Donovan M, Cordon-Cardo C, Beug H, Grunert S: Autocrine PDGFR signaling promotes mammary cancer metastasis. J Clin Invest. 2006, 116 (6): 1561-1570. 10.1172/JCI24652.View ArticlePubMedPubMed CentralGoogle Scholar
- Singh PK, Wen Y, Swanson BJ, Shanmugam K, Kazlauskas A, Cerny RL, Gendler SJ, Hollingsworth MA: Platelet-derived growth factor receptor beta-mediated phosphorylation of MUC1 enhances invasiveness in pancreatic adenocarcinoma cells. Cancer Res. 2007, 67 (11): 5201-5210. 10.1158/0008-5472.CAN-06-4647.View ArticlePubMedGoogle Scholar
- Suyama K, Shapiro I, Guttman M, Hazan RB: A signaling pathway leading to metastasis is controlled by N-cadherin and the FGF receptor. Cancer Cell. 2002, 2 (4): 301-314. 10.1016/S1535-6108(02)00150-2.View ArticlePubMedGoogle Scholar
- Xian W, Schwertfeger KL, Vargo-Gogola T, Rosen JM: Pleiotropic effects of FGFR1 on cell proliferation, survival, and migration in a 3D mammary epithelial cell model. J Cell Biol. 2005, 171 (4): 663-673. 10.1083/jcb.200505098.View ArticlePubMedPubMed CentralGoogle Scholar
- Serrels A, Macpherson IR, Evans TR, Lee FY, Clark EA, Sansom OJ, Ashton GH, Frame MC, Brunton VG: Identification of potential biomarkers for measuring inhibition of Src kinase activity in colon cancer cells following treatment with dasatinib. Mol Cancer Ther. 2006, 5 (12): 3014-3022. 10.1158/1535-7163.MCT-06-0382.View ArticlePubMedGoogle Scholar
- Morton JP, Karim SA, Graham K, Timpson P, Jamieson N, Athineos D, Doyle B, McKay C, Heung MY, Oien KA, et al: Dasatinib inhibits the development of metastases in a mouse model of pancreatic ductal adenocarcinoma. Gastroenterology. 2010, 139 (1): 292-303. 10.1053/j.gastro.2010.03.034.View ArticlePubMedGoogle Scholar
- Heldin CH, Ostman A, Ronnstrand L: Signal transduction via platelet-derived growth factor receptors. Biochim Biophys Acta. 1998, 1378 (1): F79-113.PubMedGoogle Scholar
- Dolloff NG, Shulby SS, Nelson AV, Stearns ME, Johannes GJ, Thomas JD, Meucci O, Fatatis A: Bone-metastatic potential of human prostate cancer cells correlates with Akt/PKB activation by alpha platelet-derived growth factor receptor. Oncogene. 2005, 24 (45): 6848-6854. 10.1038/sj.onc.1208815.View ArticlePubMedPubMed CentralGoogle Scholar
- Sitaras NM, Sariban E, Bravo M, Pantazis P, Antoniades HN: Constitutive production of platelet-derived growth factor-like proteins by human prostate carcinoma cell lines. Cancer Res. 1988, 48 (7): 1930-1935.PubMedGoogle Scholar
- Bornfeldt KE, Raines EW, Nakano T, Graves LM, Krebs EG, Ross R: Insulin-like growth factor-I and platelet-derived growth factor-BB induce directed migration of human arterial smooth muscle cells via signaling pathways that are distinct from those of proliferation. J Clin Invest. 1994, 93 (3): 1266-1274. 10.1172/JCI117081.View ArticlePubMedPubMed CentralGoogle Scholar
- Cattaneo MG, Gentilini D, Vicentini LM: Deregulated human glioma cell motility: inhibitory effect of somatostatin. Mol Cell Endocrinol. 2006, 256 (1-2): 34-39. 10.1016/j.mce.2006.05.006.View ArticlePubMedGoogle Scholar
- Aoki M, Nabeshima K, Koga K, Hamasaki M, Suzumiya J, Tamura K, Iwasaki H: Imatinib mesylate inhibits cell invasion of malignant peripheral nerve sheath tumor induced by platelet-derived growth factor-BB. Lab Invest. 2007, 87 (8): 767-779. 10.1038/labinvest.3700591.View ArticlePubMedGoogle Scholar
- Matsumoto S, Yamada Y, Narikiyo M, Ueno M, Tamaki H, Miki K, Wakatsuki K, Enomoto K, Yokotani T, Nakajima Y: Prognostic significance of platelet-derived growth factor-BB expression in human esophageal squamous cell carcinomas. Anticancer Res. 2007, 27 (4B): 2409-2414.PubMedGoogle Scholar
- Cao R, Bjorndahl MA, Religa P, Clasper S, Garvin S, Galter D, Meister B, Ikomi F, Tritsaris K, Dissing S, et al: PDGF-BB induces intratumoral lymphangiogenesis and promotes lymphatic metastasis. Cancer Cell. 2004, 6 (4): 333-345. 10.1016/j.ccr.2004.08.034.View ArticlePubMedGoogle Scholar
- Donnem T, Al-Saad S, Al-Shibli K, Busund LT, Bremnes RM: Co-expression of PDGF-B and VEGFR-3 strongly correlates with lymph node metastasis and poor survival in non-small-cell lung cancer. Ann Oncol. 2010, 21 (2): 223-231. 10.1093/annonc/mdp296.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/11/309/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.