Inhibition of DX3 cell invasion by E7080. (A) Invasion of DX3 cells into Matrigel in the presence or absence of E7080 was measured: DX3 cells were stained with calcien AM and images taken every 15 μm. (B) Quantification of invasion is shown for a representative experiment in a series of three. Values are mean + s.e.m. of two transwells with 4 fields per transwell. p values comparing treated cells with controls were calculated using Paired t-test. (C) The fluorescent intensity of DX3 cells on the upper side of the Transwell was measured in untreated and E7080 treated cells following staining with calcein AM. Results are mean + s.e.m from a representative experiment in a series of three.