PDGFR-β is required for U2OS cell migration. (A) Cell lysates were prepared following transfection of U2OS cells with PDGFR-β or non-targeting siRNA oligonucleotides and PDGFR-β immunoprecipitated. The immune complexes were resolved by SDS-PAGE and probed with an anti-PDGFR-β antibody. (B) Wound healing assays with U2OS cells were carried out in the presence or absence of E7080 and/or PDGFR-β siRNA oligonucleotides. Values are mean + s.e.m. from a representative experiment in a series of three, p values comparing all with control were calculated using Paired t-test. (C) Wound healing assay in DU145 cells treated with E7080. Values are mean + s.e.m. from a representative experiment in a series of three.