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Variations in Mre11/Rad50/Nbs1 status and DNA damage-induced S-phase arrest in the cell lines of the NCI60 panel
© Garner and Eastman; licensee BioMed Central Ltd. 2011
Received: 24 February 2011
Accepted: 27 May 2011
Published: 27 May 2011
The Mre11/Rad50/Nbs1 (MRN) complex is a regulator of cell cycle checkpoints and DNA repair. Defects in MRN can lead to defective S-phase arrest when cells are damaged. Such defects may elicit sensitivity to selected drugs providing a chemical synthetic lethal interaction that could be used to target therapy to tumors with these defects. The goal of this study was to identify these defects in the NCI60 panel of cell lines and identify compounds that might elicit selective cytotoxicity.
We screened the NCI60 panel in search of cell lines that express low levels of MRN proteins, or that fail to arrest in S-phase in response to the topisomerase I inhibitor SN38. The NCI COMPARE program was used to discover compounds that preferentially target cells with these phenotypes.
HCT116 cells were initially identified as defective in MRN and S phase arrest. Transfection with Mre11 also elevated Rad50 and Nbs1, and rescued the defective S-phase arrest. Cells of the NCI60 panel exhibited a large range of protein expression but a strong correlation existed between Mre11, Rad50 and Nbs1 consistent with complex formation determining protein stability. Mre11 mRNA correlated best with protein level suggesting it was the primary determinant of the overall level of the complex. Three other cell lines failed to arrest in response to SN38, two of which also had low MRN. However, other cell lines with low MRN still arrested suggesting low MRN does not predict an inability to arrest. Many compounds, including a family of benzothiazoles, correlated with the failure to arrest in S phase. The activity of benzothiazoles has been attributed to metabolic activation and DNA alkylation, but we note several cell lines in which sensitivity does not correlate with metabolism. We propose that the checkpoint defect imposes an additional mechanism of sensitivity on cells.
We have identified cells with possible defects in the MRN complex and S phase arrest, and a series of compounds that may preferentially target S phase-defective cells. We discuss limitations of the COMPARE program when attempting to identify compounds that selectively inhibit only a few cell lines.
Many anticancer agents kill cells as a consequence of damaging their DNA. While this approach targets rapidly proliferating cells, it is only somewhat selective for tumors and can lead to undesirable toxicity to the patient. Differences exist between normal and tumor cells with respect to the cellular response to DNA damage and it may be possible to exploit these differences to selectively target tumor cells. One such difference is in cell cycle checkpoint regulation. In response to DNA damage, cell cycle checkpoints are activated causing the arrest of cell cycle progression . This allows the cell time to repair the damage, or if too much damage has occurred, undergo cell death. Defects in checkpoints lead to mutations and cancer, and this genomic instability is now considered a characteristic of tumors . As the checkpoints function to protect the cells from damage, it is hoped that their selective defect in tumor cells may result in sensitivity to novel therapeutic strategies.
The S-phase checkpoint is activated in response to DNA damage that occurs during replication. For example, topoisomerase I inhibitors such as SN38 produce a single-strand break in DNA which is converted to a double-strand break upon collision with the replication fork . The MRN complex, consisting of Mre11, Rad50 and Nbs1, is recruited to the double-strand break and initiates the S-phase checkpoint by recruiting ataxia-telangiectasia-mutated (ATM) to the damaged site . Processing at the break by the Mre11 nuclease generates regions of single-stranded DNA that recruit the ATM- and Rad3-related kinase (ATR) . Activated ATR and ATM phosphorylate the checkpoint kinases Chk1 and Chk2, respectively, which in turn phosphorylate CDC25A targeting it for degradation and effectively arresting DNA synthesis through inactivation of the Cdk2/Cyclin A complex [6, 7].
Mutations in the MRN complex have been identified in lymphoid, breast and colorectal tumors [8, 9]. A defect in the MRN complex has also been identified in the colorectal tumor cell line, HCT116 . Such mutations result in radioresistant DNA synthesis (RDS) and hypersensitivity to ionizing radiation [10–12]. In addition, MRN mutations have been reported to increase sensitivity towards anticancer drugs, such as topoisomerase I inhibitors and cisplatin [13, 14].
Studies in our laboratory have confirmed that HCT116 cells have very low levels of Mre11 and also that they arrest poorly in S phase in response to SN38. By transfecting HCT116 cells with a vector expressing Mre11, we show here that we can reconstitute the MRN complex. Our data indicate that Mre11 is necessary for the stability of Rad50 and Nbs1 and rescues the S-phase arrest in HCT116.
MRN defects if present in some tumors could render these cells sensitive to DNA-damaging agents and thereby provide a tumor selective therapy. The primary goal of these studies was to find compounds that preferentially target cells with defects in the proteins of the MRN complex. We have examined the frequency of MRN defects across the NCI60 cell line panel which is derived from many different cancer types (albeit only 59 are presently available) . These cell lines have been characterized pharmacologically by exposure to more than 100,000 compounds (http://dtp.nci.nih.gov). We then correlated the MRN protein levels to sensitivity to the compounds in the NCI database using the COMPARE program [16, 17]. Since there are many other proteins involved in the S-phase checkpoint we also assessed the cell lines for their ability to arrest in S phase when incubated with SN38, and then searched the database for additional correlations.
NCI60 cancer cell lines were obtained from the Developmental Therapeutics Program at the National Cancer Institute and were cultured in RPMI 1640 media (Hyclone) supplemented with 10% heat-inactivated fetal bovine serum (Hyclone) and Antibiotic-antimycotic (Gibco). Cells were cultured at 37°C with 5% CO2.
Elevated expression of Mre11 was achieved by cloning Mre11 from pDB20 (obtained from John Petrini, Memorial Sloan-Kettering Cancer Center) into pCR3.1 (Invitrogen) . This plasmid was transfected into HCT116 cells using lipofectamine (Invitrogen), according to the manufacturer's protocol. Cells were selected in G418 (500 μg/ml) and multiple colonies were screened for maximum expression.
Drugs and chemicals
SN38, the active metabolite of irinotecan, was obtained from Pfizer, and 2-(4-amino-3-methylphenyl)-5-fluorobenzothiazole (5F 203, NSC 703786) was obtained from the Developmental Therapeutics Program at the National Cancer Institute (Bethesda, MD). Both drugs were dissolved in DMSO and stored at -20°C.
For cell cycle analysis, cells were rinsed with phosphate-buffered saline (PBS), harvested with 0.05% trypsin, and fixed in 70% ethanol overnight. Cells were stained with 100 μg/ml propidium iodide in 1 mg/ml ribonuclease A and analyzed on a FACScan flow cytometer (Becton Dickinson).
Following treatment, cells were rinsed with phosphate buffered saline, lysed by direct addition of Laemmli buffer to the wells, boiled for 5 min, and stored at -80°C. Protein levels were quantified using EZQ Protein Quantitation Kit (Molecular Probes). 15 μg of each lysate was separated by SDS-PAGE, and transferred to polyvinylidene difluoride membranes. Membranes were blocked and incubated overnight at 4°C with primary antibodies specific for Nbs1 (Cell Signaling), Rad50 (Novus Biologicals), Mre11 or actin (Calbiochem). Membranes were washed and incubated for 30 min at room temperature with anti-rabbit or anti-mouse secondary antibody conjugated to horseradish peroxidase. Proteins were visualized with enhanced chemiluminescence. Densitometry scans were quantified using Scion Image software (Scion Corporation) and normalized to a serial dilution of the same protein from MDA-MB-231 present on each blot. Several exposures of each western blot were scanned to avoid over-exposure and to ensure that the samples were within the range of the standards.
Cell growth assays
Cells were seeded at low density (500-1000 cells) in 96-well plates and then incubated with drug for 24 h. Following treatments, cells were washed and grown in fresh media for 5-7 days at 37°C. Prior to attaining confluence, cells were washed, lysed, and stained with Hoechst 33258, as previously described . Fluorescence was read on a microplate spectrofluorometer (Spectramax M2). Within each experiment, each treatment was repeated in at least eight wells. Sensitivity to the "NSC" compounds was determined by the Developmental Therapeutics program at NCI and the data were obtained from their web site (http://dtp.nci.nih.gov). In their screen, all cell lines were incubated with a range of drug concentrations, usually for 48 h, and then fixed and stained with sulforhodamine B.
Mre11 rescues S-phase arrest in HCT116 cells
MRN complex protein levels vary greatly across the cell lines of the NCI60 panel
While this analysis was designed to identify cell lines with low levels of the MRN proteins, we also identified several lines in which the ratio of these proteins far exceeded the expected values. For example, SW620 cells had about 10 fold more Mre11 than Rad50, whereas Nbs1 was intermediate between these two levels. On the other hand, HCC2998, K562 and EKVX had about 2 fold more Rad50 than Mre11. For these latter cell lines, Mre11 and Nbs1 were relatively similar suggesting that it is the elevated level of Rad50 that maybe abnormal. Whether these cell lines contain functionally defective MRN remains to be determined.
To extend this analysis further, we compared the protein levels to mRNA levels obtained from the NCI database (Figure 2C and Table S1 in Additional File 1). The correlation coefficient for Mre11 mRNA and protein was 0.55, but only 0.24 and 0.15 for Rad50 and Nbs1, respectively (Figure 3D-F). This observation suggests that Mre11 protein levels are more highly regulated by mRNA levels whereas the Rad50 and Nbs1 protein levels are relatively independent of mRNA levels and likely depend more on protein stability mediated via formation of the MRN complex. It is also important to note that most of the cells we identified as having low levels of Mre11 protein had relatively normal levels of MRN mRNA suggesting that, like HCT116, they may contain a defective protein rather than the level of protein being regulated at the transcriptional level. The one notable exception was IGROV1 which has a very low level of Mre11 mRNA; surprisingly, these cells also exhibited a very low level of mRNA for Rad50. Therefore in this case, it appears that the reduced levels of the MRN complex are a consequence of transcription.
Correlations between MRN protein levels and NCI60 compound activity
The highest correlating compounds for Mre11, Rad50 and Nbs1
Positive correlations >0.5 = 4
Positive correlations >0.5 = 44
Positive correlations >0.5 = 8
Negative correlations >0.5 = 4
Negative correlations >0.5 = 3
Negative correlations >0.5 = 8
Lack of S-phase arrest in cell lines correlates with the activity of compounds, including several benzothiazoles
Compounds with the highest correlation with SN38-induced S phase arrest
SN38-induced S phase arrest
Positive correlations >0.5 = 157
Negative correlations >0.5 = 11
The sensitivity of cells to benzothiazoles is reported to be due to the ability of cells to metabolically activate these compounds which then go on to damage DNA [22, 23]. MCF7 cells exhibit the highest metabolism of benzothiazoles and we therefore anticipated that they should sensitize non-responsive cells in co-culture. We used transwell plates in a 96 well format and plated ~50,000 MCF7 cells in the upper well. Other cell lines, including HCT116, were plated at low density in the lower wells. NSC703783 (1 μM) was added for 24 h then removed, in an experiment similar to that in Figure 9. The MCF7 cells had no impact on the growth of six cell lines tested. We conclude that differences in metabolism do not adequately explain the differences in sensitivity of cells to the benzothiazoles.
The overall goal of this study was to identify molecular defects in cell cycle checkpoint regulation using the NCI60 panel of cell lines, and then to identify compounds that might target cells with these defects. Eventually, such compounds might provide leads for the development of drugs for personalized cancer therapy. We began by screening the cell panel for potential defects in MRN proteins and the S-phase checkpoint. We uncovered a number of interesting relationships with respect to MRN proteins and their mRNA that impact the level of this complex. In addition, using the COMPARE program, we have identified several compounds of interest which warrant further study.
Our results implicate protein stability in the regulation of MRN protein levels. Transfection with Mre11 not only increased Mre11 levels but also Rad50 and Nbs1 levels. In comparing the protein levels in each cell line across the NCI60 panel, we also found a strong correlation between the levels of these three proteins. The overall level of the complex appeared to be primarily regulated by transcription of Mre11 as there was much lower correlation between mRNA and protein levels for Rad50 and Nbs1. These results suggest that the protein levels of Rad50 and Nbs1 are primarily regulated by stability of the complex while transcription of Mre11 contributes more to the overall level of the complex.
While Mre11 expression may be more highly regulated by transcription, among the cell lines with low levels of Mre11 protein, only the IGROV1 cells had a very low level of mRNA. HCT116 had a relatively high level of Mre11 transcript but low protein, and this has been shown to be due to a splicing defect in its mRNA . This defect occurs as a frame shift in a poly(dA) track in an intron which is consistent with these cells being defective for mismatch repair MLH1 [9, 24]. Five cell lines in the NCI60 panel are defective for MLH1 , one of which is IGROV1, but the other three (SKOV3, KM12 and CCRF-CEM) still express relatively normal levels of Mre11. None of the NCI cell lines are defective in the mismatch gene MSH2. Hence mismatch repair defects do not appear to explain the low levels of Mre11 protein in these other cell lines. Other mutations which might result in protein degradation could be a possible cause for the low protein levels in the other cells but further study is necessary. In addition, there were several cell lines with unbalanced levels of MRN, for example, unexpected high or low levels of Rad50 compared to Mre11. One possible explanation for this observation is that the proteins contain a mutation which confers greater protein stability when they are not in the MRN complex.
This screen identified a small number of compounds which correlated with either Mre11, Rad50, or Nbs1. However, there were three compounds identified which correlated with both Mre11 and Rad50. The activity of one of these, NSC 734406, was significant at the concentrations tested and correlates with low levels of Mre11 and Rad50 (Figure 4). Validation and further study with this compound is necessary to assess whether it does preferentially kill cells with low MRN protein but this was not possible as the compound is not available from the NCI repository. One of the potential limitations of this COMPARE analysis is that protein levels may not necessarily reflect MRN function, particularly in those cases where MRN protein expression is unbalanced. Another approach would be to assay the cells for MRN activity using a functional readout, such as Chk1 phosphorylation in response to ionizing radiation, for which the MRN complex is required . Alternately the recruitment of ATM to damaged DNA could be assayed as this also depends on the MRN complex.
We have previously reported an alternate means to prevent S phase arrest . The expression of shRNA against Chk1 in MDA-MB-231 cells overcame the S phase arrest induced by SN38, although such cells still arrested in G2. We observed a similar response when cells were co-incubated with SN38 and a small molecule inhibitor of Chk1. There is a close link between the MRN complex and Chk1 activation. Specifically, MRN is recruited to double-strand breaks where the exonuclease activity of Mre11 creates single-stranded DNA that, through a series of events, recruits ATR and activates Chk1. However, it has been shown that HCT116 cells are fully competent to activate Chk1 when damaged with topoisomerase I inhibitors  and we have confirmed this in our own laboratory (data not shown). This is likely explained by the topoisomerase I inhibitors stalling replication fork progression thereby activating ATR and Chk1 without the need to implicate ATM and Mre11. This raises the conundrum as to why SN38 is fully capable of activating Chk1 in HCT116 cells yet the cells fail to S phase arrest unless they are complemented with Mre11. The results suggest that activation of Chk1 may be necessary but not sufficient to induce S phase arrest and that the MRN complex plays some additional role.
In searching for correlations between S-phase checkpoint defects and compound activity, we identified many drugs which correlated positively, meaning these compounds have activity in cell lines with defects. Six compounds out of the top 30 correlations identified mapped to the same cluster indicating that they share the same mechanism of growth inhibition. Four of these are benzothiazoles, a class of compounds that has been shown to be metabolized by sensitive cells and then form DNA adducts [22, 23]. Whether this is also true of other compounds in this cluster has not been determined. However, we note that KM12 cells have previously been shown to metabolize benzothiazoles, yet they are insensitive to these compounds . Similarly we note that TK10 cells are as sensitive as MCF7, yet have markedly lower level of metabolism . The lack of sensitivity of HCT116 to benzothiazoles may be due to their inability to metabolize the compound, however HCT116 cells (and many others) remained insensitive to benzothiazoles when co-cultured with MCF7 cells. Accordingly, while metabolism may contribute to the sensitivity to these compounds in some cells, there may also be a major contribution from the S-phase checkpoint defect.
The fact that the S-phase checkpoint search was a functional assay provides greater confidence in the candidates identified than with the MRN protein screen. However, because the S-phase defect can be caused by multiple reasons (e.g., low or defective ATM, ATR, Mre11 or Chk1), we may have missed many potential drug candidates. Some compounds may correlate with only some of the checkpoint defects responsible for the lack of SN38-induced S-phase arrest. The fact that each defect may be rare in the NCI60 panel makes it a challenge to use this approach to identify novel drug candidates. For example, a compound that has the same activity in 60 cell lines would exhibit a very high correlation with a dataset in which 56 cell lines arrested and 4 did not, the correlation coefficient being driven by the 56 cell lines rather than the 4 interesting queries. Accordingly, each compound discovered in the screen had to be individually analyzed and validated. Many compounds elicited no obvious correlation with the query set. In some cases (e.g., NSC358873), three of the four cell lines that failed to arrest in S phase had not even been screened against this compound. These limitations are unfortunate because we had hoped to use this approach to identify compounds that killed only a few cell lines, and might therefore be candidates for selective therapy for tumors with that particular phenotype (i.e., individualized therapy). Given the limitations of this screen, the fact that most of the cells defective for S phase arrest were indeed sensitive to compounds in the benzothiazole cluster gives strong support for further investigation into this class of drugs.
Genomic instability is one of the characteristics of cancer and is frequently attributable to defects in DNA damage response genes involved in either DNA repair or checkpoint regulation. This provides a therapeutic opportunity as these defective cells may be hypersensitive to various drugs. We have identified cells with potential defects in the MRN complex and in S phase arrest, and a series of compounds that may preferentially target S phase arrest-defective cells. The in silico screen used has limitations when seeking compounds that are cytotoxic to only a few cell lines. But considering this limitation, the identification of benzothiazoles as compounds that preferentially target cells with a defect in S phase arrest warrants further investigation. Compounds such as these that kill only a few cell lines likely represent the future of individualized cancer therapeutics.
We thank John Petrini, Memorial Sloan Kettering Cancer Institute, New York for providing the Mre11 cDNA, and the National Cancer Institute's Developmental Therapeutics Program for providing the 59 cell lines that were studied. This work was supported by a research grant from the National Institutes of Health (CA117874), and a Cancer Center Support grant to the Norris Cotton Cancer Center (CA23108). Kristen Garner was supported by an NIH training grant (T32 09658).
- Hartwell LH, Weinert TA: Checkpoints: controls that ensure the order of cell cycle events. Science. 1989, 246: 629-634. 10.1126/science.2683079.View ArticlePubMedGoogle Scholar
- Luo J, Solimini NL, Elledge SJ: Principles of cancer therapy: oncogene and non-oncogene addiction. Cell. 2009, 136: 823-837. 10.1016/j.cell.2009.02.024.View ArticlePubMedPubMed CentralGoogle Scholar
- Pommier Y: Topoisomerase I inhibitors: camptothecins and beyond. Nature Rev Cancer. 2006, 6: 789-802. 10.1038/nrc1977.View ArticleGoogle Scholar
- Lee JH, Paull TT: ATM activation by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex. Science. 2005, 308: 551-554. 10.1126/science.1108297.View ArticlePubMedGoogle Scholar
- Jazayeri A, Falck J, Lukas C, Bartek J, Smith GC, Lukas J, Jackson SP: ATM- and cell cycle-dependent regulation of ATR in response to DNA double-strand breaks. Nature Cell Biol. 2006, 8: 37-45. 10.1038/ncb1337.View ArticlePubMedGoogle Scholar
- Mailand N, Falck J, Lukas C, Syljuasen RG, Welcher M, Bartek J, Lukas J: Rapid destruction of human Cdc25A in response to DNA damage. Science. 2000, 288: 1425-1429. 10.1126/science.288.5470.1425.View ArticlePubMedGoogle Scholar
- Falck J, Mailand N, Syljuasen RG, Bartek J, Lukas J: The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis. Nature. 2001, 410: 842-847. 10.1038/35071124.View ArticlePubMedGoogle Scholar
- Fukuda T, Sumiyoshi T, Takahashi M, Kataoka T, Asahara T, Inui H, Watatani M, Yasutomi M, Kamada N, Miyagawa K: Alterations of the double-strand break repair gene MRE11 in cancer. Cancer Res. 2001, 61: 23-26.PubMedGoogle Scholar
- Giannini G, Ristori E, Cerignoli F, Rinaldi C, Zani M, Viel AOttini L, Crescenzi M, Martinotti S, Bignami M, Frati L, Screpanti I, Gulino A: Human MRE11 is inactivated in mismatch repair-deficient cancers. EMBO reports. 2002, 3: 248-254. 10.1093/embo-reports/kvf044.View ArticlePubMedPubMed CentralGoogle Scholar
- Young BR, Painter RB: Radioresistant DNA synthesis and human genetic diseases. Hum Genet. 1989, 82: 113-117. 10.1007/BF00284040.View ArticlePubMedGoogle Scholar
- Stewart GS, Maser RS, Stankovic T, Bressan DA, Kaplan MI, Jaspers NG, Raams A, Byrd PJ, Petrini JH, Taylor AM: The DNA double-strand repair gene hMre11 is mutated in individuals with an ataxia-telanciectasia-like disorder. Cell. 1999, 99: 577-587. 10.1016/S0092-8674(00)81547-0.View ArticlePubMedGoogle Scholar
- Luo G, Yao MS, Bender CF, Mills M, Bladl AR, Bradley A, Petrini JH: Disruption of mRad50 causes embryonic stem cell lethality, abnormal embryonic development, and sensitivity to ionizing radiation. Proc Natl Acad Sci USA. 1999, 96: 7376-7381. 10.1073/pnas.96.13.7376.View ArticlePubMedPubMed CentralGoogle Scholar
- Rodriguez R, Hansen LT, Phear G, Scorah J, Spang-Thomsen M, Cox A, Helleday T, Meuth M: Thymidine selectively enhances growth suppressive effects of camptothecin/irinotecan in MSI+ cells and tumors containing a mutation in Mre11. Clin Cancer Res. 2008, 14: 5476-5483. 10.1158/1078-0432.CCR-08-0274.View ArticlePubMedGoogle Scholar
- Tran HM, Shi G, Li G, Carney JP, O'Malley B, Li D: Mutant Nbs1 enhances cisplatin-induced DNA damage and cytotoxicity in head and neck cancer. Otolaryngol Head Neck Surg. 2004, 131: 477-484. 10.1016/j.otohns.2004.04.019.View ArticlePubMedGoogle Scholar
- Weinstein JN, Myers TG, O'Connor PM, Friend SH, Fornace AJ, Kohn KW, Fojo T, Bates SE, Rubinstein LV, Anderson NL, Buolamwini JK, van Osdol WW, Monks AP, Scudiero DA, Sausville EA, Zaharevitz DW, Bunow B, Viswanadhan VN, Johnson GS, Wittes RE, Paull KD: An information-intensive approach to the molecular pharmacology of cancer. Science. 1997, 275: 343-349. 10.1126/science.275.5298.343.View ArticlePubMedGoogle Scholar
- Monga M, Sausville EA: Developmental therapeutics program at the NCI: molecular target and drug discovery process. Leukemia. 2002, 16: 520-526. 10.1038/sj.leu.2402464.View ArticlePubMedGoogle Scholar
- Zaharevitz DW, Holbeck SL, Bowerman C, Svetlik PA: COMPARE: a web accessible tool for investigating mechanisms of cell growth inhibition. J Mol Graph Model. 2002, 20: 297-303. 10.1016/S1093-3263(01)00126-7.View ArticlePubMedGoogle Scholar
- Becker DM, Fikes JD, Guarente L: A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast. Proc Natl Acad Sci USA. 1991, 88: 1968-1972. 10.1073/pnas.88.5.1968.View ArticlePubMedPubMed CentralGoogle Scholar
- Rao J, Otto WR: Fluorometric DNA assay for cell growth estimation. Anal Biochem. 1992, 207: 186-192. 10.1016/0003-2697(92)90521-8.View ArticlePubMedGoogle Scholar
- Levesque AA, Fanous AA, Poh A, Eastman A: Defective p53 signaling in p53 wildtype tumors attenuates p21waf1 induction and cyclin B repression rendering them sensitive to Chk1 inhibitors that abrogate S and G2 arrest. Mol Cancer Therap. 2008, 7: 252-262. 10.1158/1535-7163.MCT-07-2066.View ArticleGoogle Scholar
- Takemura H, Rao VA, Sordet O, Furuta T, Miao ZH, Meng L, Zhang H, Pommier Y: Defective Mre11-dependent activation of Chk2 by Ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem. 2006, 281: 30814-30823. 10.1074/jbc.M603747200.View ArticlePubMedGoogle Scholar
- Kashiyama E, Hutchinson I, Chua MA, Stinson SF, Phillips LR, Kaur G, Sausville EA, Bradshaw TD, Westewll AD, Stevens MFG: Antitumor benzothiazoles. 8. Synthesis, metabolic formation, and biological properties of the C- and N-oxidation products of antitumor 2-(4-aminophenyl)-benzothiazoles. J Med Chem. 1999, 42: 4172-4184. 10.1021/jm990104o.View ArticlePubMedGoogle Scholar
- Leong CO, Gaskell M, Martin EA, Heydon RT, Farmer PB, Bibby MC, Cooper PA, Double JA, Bradshaw TD, Stevens MF: Antitumour 2-(4-aminophenyl)benzothiazoles generate DNA adducts in sensitive tumor cells in vitro and in vivo. Br J Cancer. 2003, 88: 470-477. 10.1038/sj.bjc.6600719.View ArticlePubMedPubMed CentralGoogle Scholar
- Wen Q, Scorah J, Phear G, Rodgers G, Rodgers S, Meuth M: A mutant allele of Mre11 found in mismatch repair-deficient tumor cells suppresses the cellular response to DNA replication fork stress in a dominant negative manner. Mol Biol Cell. 2008, 19: 1693-1705. 10.1091/mbc.E07-09-0975.View ArticlePubMedPubMed CentralGoogle Scholar
- Taverna P, Liu L, Hanson AJ, Monks A, Gerson SL: Characterization of MLH1 and MSH2 DNA mismatch repair proteins in cell lines of the NCI anticancer drug screen. Cancer Chemother Pharmacol. 2000, 46: 507-516. 10.1007/s002800000186.View ArticlePubMedGoogle Scholar
- Myers JS, Cortez D: Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem. 2006, 281: 9346-9350.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang WH, Poh A, Fanous AA, Eastman A: DNA damage-induced S phase arrest in human breast cancer depends on CHK1, but G2 arrest can occur independently of Chk1, Chk2 or MAPKAPK2. Cell Cycle. 2008, 7: 1668-1677. 10.4161/cc.7.11.5982.View ArticlePubMedGoogle Scholar
- Aiello S, Wells G, Stone EL, Kadri H, Bazzi R, Bell DR, Stevens MF, Matthews CS, Bradshaw TD, Westwell AD: Synthesis and biological properties of benzothiazole, benzoxazole, and chromen-4-one analogues of the potent antitumor agents 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (PMX 610, NSC 721648. J Med Chem. 2008, 51: 5135-5139. 10.1021/jm800418z.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/11/206/prepub
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