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Figure 2 | BMC Cancer

Figure 2

From: Variations in Mre11/Rad50/Nbs1 status and DNA damage-induced S-phase arrest in the cell lines of the NCI60 panel

Figure 2

MRN protein and mRNA levels in the NCI60 panel. (A) Cell lines of the NCI60 panel were lysed and Mre11, Rad50, and Nbs1 proteins were analyzed by western blot. Lysates from each cell line were loaded in duplicate and three concentrations of MDA-MB-231 were loaded in duplicate on each gel. A representative blot is shown. (B) Multiple exposures of the western blots were scanned and quantified using Scion Image software. Protein levels were normalized to MDA-MB-231 = 1. (C) mRNA levels for Mre11, Rad50 and Nbs1 were derived from an Affymetrix human genome U133A-B array and obtained from the NCI Developmental Therapeutics Program database (http://dtp.nci.nih.gov; experiment ID# 223469). Values were normalized to MDA-MB-231 = 1. The numerical values for the data in panels B and C are provided as Table S1 in Additional file 1.

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