- Research article
- Open Access
Differential cytotoxicity induced by the Titanium(IV)Salan complex Tc52 in G2-phase independent of DNA damage
BMC Cancer volume 16, Article number: 469 (2016)
Chemotherapy is one of the major treatment modalities for cancer. Metal-based compounds such as derivatives of cisplatin are in the front line of therapy against a subset of cancers, but their use is restricted by severe side-effects and the induction of resistance in treated tumors. Subsequent research focused on development of cytotoxic metal-complexes without cross-resistance to cisplatin and reduced side-effects. This led to the discovery of first-generation titanium(IV)salan complexes, which reached clinical trials but lacked efficacy. New-generation titanium (IV)salan-complexes show promising anti-tumor activity in mice, but their molecular mechanism of cytotoxicity is completely unknown.
Four different human cell lines were analyzed in their responses to a toxic (Tc52) and a structurally highly related but non-toxic (Tc53) titanium(IV)salan complex. Viability assays were used to reveal a suitable treatment range, flow-cytometry analysis was performed to monitor the impact of dosage and treatment time on cell-cycle distribution and cell death. Potential DNA strand break induction and crosslinking was investigated by immunostaining of damage markers as well as automated fluorometric analysis of DNA unwinding. Changes in nuclear morphology were analyzed by DAPI staining. Acidic beta-galactosidase activity together with morphological changes was monitored to detect cellular senescence. Western blotting was used to analyze induction of pro-apoptotic markers such as activated caspase7 and cleavage of PARP1, and general stress kinase p38.
Here we show that the titanium(IV)salan Tc52 is effective in inducing cell death in the lower micromolar range. Surprisingly, Tc52 does not target DNA contrary to expectations deduced from the reported activity of other titanium complexes. Instead, Tc52 application interferes with progression from G2-phase into mitosis and induces apoptotic cell death in tested tumor cells. Contrarily, human fibroblasts undergo senescence in a time and dose-dependent manner. As deduced from fluorescence studies, the potential cellular target seems to be the cytoskeleton.
In summary, we could demonstrate in four different human cell lines that tumor cells were specifically killed without induction of major cytotoxicity in non-tumorigenic cells. Absence of DNA damaging activity and the cell-cycle block in G2 instead of mitosis makes Tc52 an attractive compound for further investigations in cancer treatment.
Cancer is the second most frequent cause of death in industrial countries. Treatments to cure the disease range from classical surgery, high-energy irradiation [1–3] or DNA damaging drugs [4–9] and chemicals interfering with DNA repair [10–12] to compounds tackling signaling cascades [13–18] or the cytoskeleton [19–21] and combinations thereof. One success story was the discovery of cisplatin as chemotherapeutic agent . Platinum-compounds still play a role in chemotherapy, but their efficiency is limited to a minor cancer-panel and hampered by severe side-effects and acquired resistance . Current research focuses on other metal-complexes, with little cross-reactivity to cisplatin and reduced side-effects. Several cytotoxic titanium-based complexes have been investigated, with titanocene-dichloride and budotitane reaching clinical trials [24–27]. Unfortunately, both substances display a fast rate of hydrolysis , resulting in low efficacy and cancelling of phase-II trials. Titanium(IV)salans displaying much longer half-life in aqueous environments  and an antitumor-efficacy comparable to cisplatin [29, 30], but with no cross-resistance . Apoptosis induction by two differently substituted complexes in tumor cell lines was described , and efficacy was shown in a mouse tumor-model . Titanium(IV)salans mode of action is unknown, but titanocene-dichloride was enriched in chromatin regions , bound to DNA and inhibited DNA-synthesis and topoisomerase II . In order to elucidate the cytotoxic mechanism of titanium(IV)salans, we treated four different human cell lines with the cytotoxic compound Tc52  or the - despite a high degree in structural identity - non-toxic Tc53 [34, 35] (Additional file 1: Figure S1). We could not detect any signs of DNA damage, but cytotoxicity was selective for the tumorigenic cell lines investigated, accompanied by a G2-phase cell-cycle block. Non-tumor cells were spared from death, and fibroblasts underwent senescence. By analyzing different mitosis-targeting toxins together with Tc52, we could show that the putative target is the cytoskeleton.
DNA damage is not induced by Tc52
We investigated titanium-compounds Tc52 and Tc53 in the well-established HeLa cancer cell line and in normal VH7 fibroblasts. Both cell lines displayed similar sensitivity in viability to Tc52 (EC50 and EC80 of 6 μM and 10 μM in HeLa, and of 3.5 μM and 10 μM in VH7, respectively), whereas Tc53 had no effect (Additional file 2: Figure S2). This is interesting, as Tc53 has a very similar overall structure and differs from Tc52 only by the exchange of four methyl-groups against tert-butyl groups. Major impact of Tc52 was detected after 48 h incubation, similar to published results . As titanocene-dichloride was reported to interact with DNA , we investigated for direct or indirect induction of DNA-damage by Tc52. We monitored for the appearance of DNA-break markers poly(ADP-ribose) (PAR) and phosphorylation of histone H2AX (γH2AX), using H2O2 as positive control (Additional file 3: Figure S3A/B). Neither Tc52 (Fig. 1a/b) nor Tc53 (data not shown) induced any DNA strand-break marker up to 6 h of continuous treatment. However, after 24 h a very small subset of HeLa cells displayed γH2AX induction, coinciding with the onset of cell death. Patterns of γH2AX formation in western-blots of HeLa cell extracts after Tc52, Tc53 or H2O2 application mirrored the results from immunofluorescence studies (Additional file 3: Figure S3C). To detect DNA breaks and crosslinks, we employed the sensitive automated FADU and reverseFADU methods [36, 37]. Mitomycin C as crosslinking agent showed the expected dose-dependent fluorescence increase in reverseFADU (Fig. 1c), whereas Tc53 (10 μM) was negative (Fig. 1d). In line with data from immunofluorescence and western-blotting, 10 μM Tc52 neither induced DNA-breaks nor crosslinks (Fig. 1e). In summary, we could not detect any Tc52-dependent DNA breakage or crosslinking activity.
Tc52 blocks cells in G2-phase
We performed flow-cytometry analyses of HeLa cells after 30 h and 48 h of continuous treatment with different Tc52 concentrations to monitor for potential interference with cell-cycle progression (Fig. 2, see Additional file 4: Figure S4 for representative histograms). We noticed a profound toxic effect (subG1-fraction, up to 30-fold induction) with increased concentration and incubation time, accompanied by loss of G1-cells (2-fold). At 2 μM and higher, G2/M content increased significantly (1.5-fold), but dropped below control values at 10 μM. Tc53 had no impact on cell-cycle profile. As Tc52-dependent G2/M-accumulation may have been the result from interference in mitotic progression, we combined Tc52 treatment with toxins blocking important steps in mitosis and cytokinesis, i.e., actin-targeting cytochalasin B (CytB) or tubulin-targeting colcemid (Col) and docetaxel (Doc). To study potential interactions between Tc52 and the toxins, viability assays in HeLa were performed using single agents or combinations with 6 μM Tc52 (EC50) for 24 h to reduce Tc52-dependent cell death (Fig. 3). Under these conditions, Tc52 induced a 23 % loss in viability. To analyze the interaction mode of the combined compounds (antagonistic/additive/synergistic), we employed the algorithm of Chou and Talalay . As evident from Fig. 3a, cytotoxicity of CytB and Tc52 was independent, leading to additive loss of viability. Tc52 blocked toxicity at high concentrations of tubulin-targeting Col and Doc (antagonistic), suggesting that Tc52 may target the very same structure. We performed cell-cycle analysis in cells exposed to toxins alone or in combination, i.e., 6 μM Tc52, 4 μM CytB, 27 nM Col and 50 nM Doc, respectively (Fig. 3b, see Additional file 5: Figure S5 for representative histograms). Tc52 treatment for 24 h did not change cell-cycle profile, whereas Col-treated cells mildly accumulated in G2/M (1.5-fold), which was more pronounced by CytB (1.8-fold) and even further by docetaxel with more than 60 % in G2/M (2.9-fold). An increase of cells with a DNA content >4 N was detected in CytB-treated cultures (5.3-fold), most likely due to repressed cytokinesis, which leads to formation of binuclear cells. Doc also induced this fraction significantly (2-fold). All single treatments with CytB (6.7-fold), Col (12.3-fold) or Doc (16.9-fold) induced a subG1 peak, an indicator of cell death, and a reduction in G1 cell count (28.8-fold, 1.5-fold, and 23.2-fold, respectively) Tc52 addition reduced the percentage of cells with >4 N in Tc52-docetaxel samples compared to Doc alone (1.6-fold of control). The increase of cells with >4 N induced by CytB was severely decreased by 50 % with a concomitant increase in G2/M- and subG1-fractions (2.5-fold and 9.2-fold compared to control, respectively). To discriminate between mitosis and G2-phase, we treated cells as above and determined the mitotic index (MI) (Fig. 3c). As expected, Col and Doc caused an increase in MI by twofold and more than 11-fold, respectively. In contrast, CytB reduced MI more than threefold. Tc52 treatment alone induced a complete absence of mitotic figures. Combination of Tc52 with the toxins severely reduced the MI below normal control values, which indicates that Tc52 acts in advance of Col or Doc. To monitor the impact of Tc52 on cytoskeletal organization, we performed immunofluorescence co-staining for tubulin and actin (Fig. 3d). As expected, CytB induced binuclear cells (asterisk) with normal tubulin network, and actin was partially aggregated as detected by phalloidin-staining. Col blocked metaphases (closed arrow) with impaired mitotic spindles and normal actin network. Doc induced multipolar spindles in cells arrested in mitosis (closed arrow), and lobed nuclei in cells escaping it. Tubulin was strongly stained and arranged as straight fibers. Tc52-treated cells displayed slightly diminished phalloidin-staining with granules, and tubulin bundles in cellular protrusions (open arrows). Co-treatments showed deranged actin and tubulin networks. Induction of binuclear cells by CytB, metaphase arrest by Col, or fixed mitosis/lobed nuclei by Doc were no longer detectable when Tc52 was co-applied as expected. In summary, this indicates that Tc52 interferes with a step before progression into mitosis, i.e., during G2-phase.
Tc52 induces apoptosis in cancer cells and senescence in fibroblasts
In a next step, we investigated whether Tc52 pulse-treatment might be sufficient to induce these effects (Fig. 4). We incubated HeLa and VH7 for 6 h with increasing Tc52 concentrations and analyzed after 30 h the cell-cycle distribution by flow-cytometry. Whereas HeLa displayed major cytotoxicity at 10 μM with a loss of G1-cells (3-fold), fibroblasts showed no cell death as indicated by an unchanged subG1-fraction. Instead, cells in G1 gradually decreased (1.4-fold) concomitant with an accumulation in G2/M (1.5-fold) (Fig. 4a/b, see Additional file 6: Figure S6 for representative histograms), suggesting a differential response to Tc52 in tumorigenic HeLa and untransformed VH7 fibroblasts. To analyze this in more detail, HeLa and VH7 were treated for 2 h, 6 h, or 30 h with 2 μM or 10 μM Tc52 and nuclei were monitored for apoptosis or other alterations after a total of 30 h (Fig. 4c-f). HeLa displayed after the 2 h exposure to 10 μM Tc52 a sevenfold increase in apoptotic figures (Fig. 4c/e), which doubled with prolonged treatment, but no additional changes in nuclear morphology. VH7 displayed only at 30 h of 10 μM Tc52 a minor increase in apoptotic cells (Fig. 4d/f). In contrast, chromatin in VH7 nuclei structurally changed and DAPI-dense regions appeared, dependent on Tc52 concentration and exposure time (Fig. 4g). These foci very much resembled senescence associated heterochromatic foci (SAHF) [39, 40], which are indicators of cellular senescence. To further investigate on this, we analyzed senescence-associated beta-galactosidase (SAβGal)-activity, an enzymatic hallmark of senescence [40, 41]. Using the same pulse-treatment setup as before, we observed a time- and dose-dependent cell-enlargement, accompanied by increasing SAβGal-activity (Fig. 5a). Tc53-treated cells were undistinguishable from controls. There was a clear gain in positive staining with time and concentration of Tc52 (Fig. 5b). These results provide solid evidence that Tc52 induces senescence in fibroblasts. To support our observations about cell-specific toxicity of Tc52 by an alternative approach, we employed a recently established method to analyze cytotoxic-potential of compounds by measuring changes in free cytosolic Ca2+-levels by Fluo4-NW-dye . We exposed HeLa and VH7 to 2 μM or 10 μM Tc52 or 10 μM Tc53, and monitored for increased free cytosolic Ca2+ (Additional file 7: Figure S7, for methods see Additional file 8). In line with the cell-cycle and DAPI analysis, HeLa displayed a rapid increase in Ca2+-dependent fluorescence at both Tc52 concentrations (Δ4-RFU and Δ5.5-RFU after 5 s/ Δ6.5-RFU and Δ5-RFU after 10 s, respectively). In contrast, free Ca2+ in VH7 increased only marginally and very transient over control at 10 μM (Δ1.1-RFU after 5 s, Δ0-RFU after 10 s). 10 μM Tc53 was indistinguishable from controls. To strengthen our observation of cell-line specific response in toxicity, we treated cultures with Tc52 or Tc53 for 30 h and analyzed phosphorylation of the stress-associated p38 MAP-kinase (p-p38) and the appearance of cleaved caspase7, a sign for ongoing apoptosis. Both HeLa and VH7 showed an elevation in p-p38, but only HeLa displayed pronounced induction of active caspase7 (Fig. 6a-d), whereas Tc53 induced neither p-p38 nor caspase7-cleavage (Additional file 9: Figure S8). To validate these data, we monitored the cleavage of poly(ADP-ribose) polymerase1 (PARP1), a well-established event in early execution phase [43, 44]. Apoptotic PARP1 85 kDa-fragment was significantly induced in HeLa, but not in VH7 fibroblasts (Fig. 6). Identical response-patterns were observed in U2OS cancer cells and low-passage HEK293 (Fig. 7), with the latter being immortal but non-tumorigenic . Viability loss induced by Tc52 was similar compared to HeLa or VH7, with EC50 values of 2 μM (U2OS) and 4 μM (HEK293) (Additional file 10: Figure S9). In both cell lines p-p38 increased in a dose- and time-dependent manner, but only U2OS cells displayed activation of caspase7 and PARP1-cleavage (Fig. 7). In summary, Tc52 induced a p-p38 related stress signaling in all cell lines, but only cancer cells responded with apoptosis.
Efficacy of cancer treatment relies on higher sensitivity of tumor cells towards chemotherapeutic agents compared to surrounding tissue. The search for metal-based chemotherapeutics other than cisplatin-derivatives led to the discovery of titanium as potential replacement, with titanocene-dichloride and budotitane reaching clinical trials. Unfortunately, both compounds lacked anti-tumorigenic potential due to rapid hydrolysis in aqueous solutions . Titanium(IV)salans are much more stable  and have been shown to display promising activity against cancer cells in vitro as well as in mouse models, including some selectivity for tumor cells . But the underlying mechanism of cytotoxicity and the cellular targets have not been defined yet. We investigated this for the toxic titanium(IV)salan Tc52 and the non-toxic Tc53, which display a high degree of structural similarity with only small variations, in different human cell lines. Viability was similarly affected by Tc52 in all lines analyzed, with EC50 values between 2 μM and 6 μM Tc52 (Additional file 2: Figure S2 and Additional file 10: Figure S9), whereas Tc53 had no impact. This discrepancy in toxicity cannot be attributed to differential uptake, as similar timing in cellular accumulation has been reported for related titanium(IV)complexes . Importantly, viability assays do not discriminate between cytotoxic and cytostatic effects, as in both cases cell numbers are reduced compared to controls. Deduced from titanocene-dichloride , we expected genomic DNA to be the target of Tc52. We monitored for DNA strand-break induction with a variety of different assays, using the very sensitive FADU and reverseFADU method able to detect strand-breaks induced by 0.13 Gy of X-ray  as well as DNA crosslinks , respectively, and checked for the appearance of the two different damage markers PAR and γH2AX. Whereas synthesis of PAR is an early marker for DNA strand-breaks, detectable within seconds to minutes , γH2AX foci appear later and correlate finally with the amount of double-strand breaks . We failed to observe any DNA damage induction (Fig. 1) after Tc52 application in contrast to H2O2 treatment (Additional file 3: Figure S3). These data exclude that nuclear DNA is targeted by Tc52. Analyzing the cell-cycle profile of HeLa after Tc52 treatment revealed major cell death induction concomitant with a transient block in G2/M-phase (Fig. 2). A reasonable explanation could be that Tc52 interferes with a step during mitosis or cytokinesis, similar to biological toxins such as cytochalasin B from fungi, colchicine from autumn crocus or taxanes from yew. CytB induces binuclear cells as it interferes with formation of the contractile actin-ring in cytokinesis. In contrast, the colchicine-derivate colcemid or the taxane docetaxel target tubulin, leading to metaphase arrest, but whereas Col prevents tubulin polymerization, Doc blocks disassembly of tubulin. As a consequence of taxane treatment, multipolar spindles are induced, and cells escaping mitotic block show characteristic lobed or fragmented nuclei . Tc52 was applied in combination with all three compounds. Tc52 toxicity was additive to CytB in viability assays, but protected from cytotoxicity of higher doses of Col and Doc (Fig. 3a). Whereas addition of Tc52 mildly changed cell-cycle profile of Col and Doc treated samples, the percent of cells containing >4 N DNA content, i.e., binucleated cells, was drastically reduced in CytB/Tc52-treated cells (Fig. 3b). DAPI-staining of nuclei revealed that addition of Tc52 led to a severe drop in mitotic index (Fig. 3c). In concert with the cell-cycle analysis, these data indicate that Tc52 is not affecting mitosis, but targets an unknown step in G2, preventing progression into M-phase. We hypothesize that steps before or at G2-M transition as centrosome-separation, kinase-signaling or the cytoskeleton might be affected. Interestingly, it has been reported that tubulin targeting colcemid has two different modes of action: At low doses it inhibits proper plus-end dynamics and impacts on cell migration, whereas at high doses it seems to induce cell death by microtubule fragmentation during mitosis . Indeed, visualization of tubulin and F-actin indicated that Tc52 induced small alterations in the tubulin network, displaying increased bundling at the cellular periphery also in combination with other toxins (Fig. 3d). Whether Tc52 also impacts on migration needs to be determined in future research. But probably Tc52 acts independent of this as treated cells are blocked in a step before condensation of chromosomes takes place and mitotic microtubules are formed. Actin was mildly affected by Tc52, which could be due to modified tubulin functionality. F-actin staining was severely affected by CytB as expected, reshuffling actin into bright foci without network-formation. Col and Doc affected only the tubulin network. Of note, combined Doc/Tc52 treatment "rescued" actin and tubulin network compared to Doc-only treated samples, which supports the idea of acting in advance of Doc and thus mitosis. In summary, the protection from Col- and Doc-dependent cytotoxicity, together with the cell-cycle analysis and data from immunofluorescence suggests that Tc52 acts on the same pathway as Col or Doc, but independent of actin-targeting CytB. Monitoring cell-cycle distribution after Tc52 pulse-treatment of HeLa and VH7 revealed massive apoptosis-induction in tumorigenic HeLa, whereas fibroblasts were blocked in G2/M without signs of cell death (Fig. 4a/b). Appearance of apoptotic figures in HeLa confirmed the time- and dose-dependent increase in toxicity by Tc52 (Fig. 4c/e), which was absent in VH7 as expected from cell-cycle data (Fig. 4d/f). Instead, VH7 nuclei displayed changes in chromatin structure, i.e., an increase of DAPI-dense foci resembling SAHF [39, 40] (Fig. 4d/g), together with characteristic morphological changes such as enlargement and flattening (Fig. 5). Testing for SAβGal activity confirmed a time- and concentration-dependent induction of senescence (Fig. 5). To support our findings, we analyzed the rapid release of free Ca2+ in the cytosol in HeLa and VH7 after Tc52 exposure. This method was developed to quickly determine the cytotoxic potential of a substance . Both low and high concentrations induced in HeLa a strong increase in Ca2+-dependent fluorescence, which was absent in fibroblasts (Additional file 7: Figure S7), supporting the cell-specific difference in Tc52 response. Activation of the general stress-kinase p38 by phosphorylation at T180/Y182 is central to many cell fate pathways such as cell death, senescence, differentiation and tumorigenesis (reviewed in ). In order to analyze whether Tc52 and Tc53 application leads to activation of p38, we probed for total and phosphorylated p38 in four cell lines. Indeed, Tc52 led to increased p-p38 in a time and dose-dependent manner in all of them (Figs. 6, 7). In contrast, non-cytotoxic Tc53 did not show enhanced p-p38 levels (Additional file 8: Figure S8). To confirm the observed differential impact of Tc52 on cell death, we probed for apoptosis-dependent cleavage of caspase7 . Indeed, only HeLa and U2OS displayed dose- and time-dependent activation of caspase7 (Figs. 6, 7), and Tc53 was without effect (Additional file 9: Figure S8). Supporting our data, apoptotic PARP1-cleavage was evident only in HeLa and U2OS cultures treated with Tc52, but not in VH7 and HEK293 (Figs. 6, 7).
In summary, the titanium(IV)salan Tc52 is effective with an EC50 value in the low-micromolar range. Tc52 interferes with cell-cycle progression in G2 and deduced from our data, tubulin-related signaling is targeted, as Tc52 treatment induces alterations in the tubulin network and partially rescues from cytotoxicity of tubulin-interacting toxins that induce mitotic arrest. Alternatively, Tc52 may interfere with centrosomal regulation and prevent in this way entry into mitosis, but this would not explain the differential response towards combination with actin- or tubulin-targeting toxins. The actual target of Tc52 has to be elucidated in future research. Nevertheless, as a consequence tumor cells respond with apoptotic death, whereas normal fibroblasts show induction of senescence. We hypothesize that the constant block in G2 imposed by Tc52 cannot be handled properly by tumor cells, leading to induction of apoptosis.
We provided several layers of evidence for the differential cytotoxic potential of the titanium(IV)salan Tc52 by a pathway that is not affecting genomic integrity. As proof-of-principle, we compared two well-established cancer cell lines, HeLa and U2OS, with VH7 fibroblasts and low-passage HEK293. Importantly, Tc52 does not target DNA or block progression through mitosis, which maintains genomic information and stability. In this way, there is no risk of inducing mutations in normal tissue, which may lead to the outgrowth of secondary tumors after cancer therapy . P38 stress kinase is activated in all cells, but only tumor cell lines responded with cell death as evidenced by caspase7 activation and PARP1-cleavage. Supporting this, HeLa cells treated with Tc52 displayed an increase in subG1-fraction, apoptotic figures and cytosolic Ca2+, but not human fibroblasts, which showed induction of senescence. Further exploration of the potential anti-tumorigenic effect of this new titanium(IV)salan could be of great clinical benefit. If chemotherapy would actually discriminate between tumor and normal cells, fighting cancer could be far more effective.
Cell culture and toxins
Cells were cultured in DMEM-GlutaMax (Life-Technologies)/10 % FCS/1 % PenStrep (Life-Technologies). VH7 normal fibroblasts were used from passage 20–30, HEK293 from passage 24–34, HeLa and U2OS from routine culture. Authentication of cell lines was performed by short tandem repeat (STR) DNA-typing. Toxins were applied in medium at the concentrations and for the time periods specified with appropriate solvent controls in parallel. DMSO concentrations in medium were either 0.2 % (titanium(IV)salans alone) or 0.3 % in combination treatments. Titanium(IV)salans (Chemistry-Department University of Konstanz), cytochalasinB (Sigma) and docetaxel (LC-Laboratories) were solubilized in DMSO, colcemid (KaryoMax, Life-Technologies) and H2O2 (Sigma) diluted in medium.
Cells were seeded into 96well-plates (clear bottom/white walls, Corning) and grown for 24 h. Medium was exchanged against medium containing toxins and incubated for 24 h or 48 h. Supernatant was replaced with fresh medium containing 9 μg/ml resazurin (Sigma) and incubated until medium in control wells turned purple. Fluorescence (excitation/emission 560 nm/590 nm) was measured using LS55-spectrometer (Perkin-Elmer). Each experiment was performed in technical duplicates or triplicates.
Cells were seeded 24 h in advance onto glass cover-slips. Medium was supplemented with the respective toxin and incubated for the times specified. Cells were fixed with 3.7 % formaldehyde/PBS for 20 min at room temperature (RT), washed 1x with 0.1 M glycine/PBS for 3 min at RT and permeabilized for 5 min at RT in 0.4 % TritonX100/TBS except for detection of PAR. For PAR, cells were fixed by incubation for 7 min in −20 °C methanol at 4 °C. Subsequent procedures were identical. After three washes with TBS, cells were blocked in TBS/0.3 % Tween 20/1 % BSA for 30 min at 37 °C and incubated for 1 h at 37 °C with 1.antibody diluted in blocking-solution, followed by three washes for 10 min at RT in TBS/0.3 % Tween 20. 2.antibody incubation was performed for 1 h at 37 °C in blocking-solution, followed by three washes as above. Nuclei were stained with 20 ng/ml DAPI-solution, air-dried and mounted with AquaPolymount (PolySciences). Epifluorescence pictures were taken using a Nikon-EclipseTS100 microscope and NIS-ElementsD3.2 software. Subsequent analysis was performed using ImageJv1.47n and CS-Photoshop5.
Antibodies: 10H anti-PAR , anti-γH2AX (Millipore), anti-αTubulin (Sigma), goat-anti-mouse Alexa594 (Molecular Probes, Invitrogen). Phalloidin-Atto488 anti-F-actin (Sigma) was solubilized in DMSO to 40 μM and further diluted to 400 nM.
Automated FADU and reverseFADU
Methods have been described in detail in [36, 37]. Briefly, cells were seeded 24 h before toxin treatment. After incubation with respective concentrations of mitomycinC, 10 μM Tc52 or Tc53 for the indicated times, cells were harvested and analyzed for strand-breaks by FADU or in parallel for crosslinks by reverseFADU. For reverseFADU, cells were additionally irradiated with 25 Gy X-rays before FADU procedure. Liquid handling was performed on a Genesis RSP100 robot (Tecan). Cells were lysed and unwinding of DNA was induced by alkaline conditions. After neutralization, the relative amount of double-stranded DNA in each sample was measured by SYBR-Green fluorescence (excitation/emission 492 nm/520 nm) in FLx800-fluorescence microplate-reader (Bio-TEK Company).
Cells were seeded on 10 cm-dishes and incubated for 24 h. Medium was replaced with medium containing respective toxins and incubated for the time periods indicated. In samples exposed for 6 h, medium was replaced with fresh standard medium and further incubated for 24 h. Floating cells were collected, combined with adherent cells harvested by trypsin, and pelleted by centrifugation. Cells were washed twice with ice-cold PBS, fixed by resuspending in ice-cold 70 % ethanol to a concentration of 1x106 cells/ml and stored at −20 °C. For analysis, fixed cells were pelleted by centrifugation and washed once with PBS. Pellets were suspended in PBS containing 100 μg/ml RNaseA and 25 μg/ml propidium iodide to 1x106 cells/ml. Cell-cycle distribution was monitored using FACSCantoII (BD-Biosciences) and about 10000 events were counted in each single run. Subsequent analysis was performed using Flowing-Software2.1.
SAβGal activity assay
The SAβGal-kit from Cell-Signaling was used following manufacturer's instructions. VH7 were seeded into 6well-plates and incubated for 24 h. Afterwards, medium was exchanged against medium containing Tc52 at concentrations of 2 μM/5 μM/10 μM, 10 μM Tc53 or solvent. Cells were incubated for 2 h/6 h/30 h with Tc52 before exchange of medium against standard medium. After a total incubation of 30 h, cells were fixed and SAβGal-activity assay was performed. To analyze signal intensity, bright-field photomicrographs were taken with fixed exposure time using Nikon EclipseTS100 microscope and gray-values were measured with ImageJv1.47n-software. Nuclei were counted to normalize for cell number, and gray-values/cell were calculated.
Cells were seeded on 10 cm-dishes and incubated for 24 h. Medium was replaced with medium containing respective toxins and incubated for the indicated times. After 2 h and 6 h medium was exchanged in the respective samples against fresh standard medium. After 30 h incubation of all samples, medium was removed and floating cells were harvested by centrifugation. Pelleted cells and cells on the dish were lysed in 95 °C Laemmli-buffer and combined. Equal volumes were run on SDS-gels and blotted onto nitrocellulose- (PARP1 detection) or PVDF-membrane. Membranes were blocked with TBS/0.1 % Tween20/3 % BSA/10 % non-fat dry-milk for 2 h at 37 °C and incubated overnight at 4 °C with 1.antibody diluted in TBS/0.1 % Tween20/3 % BSA. Membranes were washed thrice for 20 min at RT in TBS/0.1 % Tween20/3 % non-fat dry-milk and incubated for 1 h at RT with 2.antibody in TBS/0.1 % Tween20/3 % non-fat dry-milk. After three washes in TBS/0.1 % Tween20, ECL detection was performed (Pierce ECL-SuperSignal-Femto, ThermoFisher Scientific). Antibodies: rabbit-polyclonal anti-GAPDH (Ambion); mouse-monoclonal anti-actin (Millipore); rabbit-polyclonal anti-p38 kinase, rabbit-monoclonal anti-phospho-p38 kinase, rabbit-polyclonal anti-tubulin, mouse-monoclonal anti-cleaved caspase7 (Cell-Signaling); goat-anti-rabbit IgG-HRP, goat-anti-mouse IgG-HRP (Sigma), mouse-monoclonal anti-PARP1 C-2-10 (SantaCruz-Biotechnologies).
All experiments were performed at least three times independently. Statistics were calculated using GraphPad-Prism5 software and tests suggested by the program were used. Presented are means +/− SEM.
Col, colcemid; CytB, cytochalasin B; DAPI, 4',6-diamidino-2-phenylindole; Doc, docetaxel; FADU, Fluorometric Analysis of DNA Unwinding; MI, mitotic index; MMC, mitomycin C; PAR, poly(ADP-ribose); PARP1, poly(ADP-ribose)polymerase1; p-p38, phosphorylated p38 stress-kinase; RT, room temperature; SAHF, senescence-associated heterochromatic foci; SAβGal, senescence-associated beta-galactosidase
Lohia S, Rajapurkar M, Nguyen SA, Sharma AK, Gillespie MB, Day TA. A Comparison of Outcomes Using Intensity-Modulated Radiation Therapy and 3-Dimensional Conformal Radiation Therapy in Treatment of Oropharyngeal Cancer. JAMA Otolaryngol Head Neck Surg. 2014.
Durante M. New challenges in high-energy particle radiobiology. Br J Radiol. 2014.
Mima M, Demizu Y, Jin D, Hashimoto N, Takagi M, Terashima K, Fujii O, Niwa Y, Akagi T, Daimon T, et al. Particle therapy using carbon ions or protons as a definitive therapy for patients with primary sacral chordoma. Br J Radiol. 2014;87(1033):20130512.
Puisset F, Schmitt A, Chatelut E. Standardization of chemotherapy and individual dosing of platinum compounds. Anticancer Res. 2014;34(1):465–70.
Griffiths TR. Current perspectives in bladder cancer management. Int J Clin Pract. 2013;67(5):435–48.
Zhu W, Zhou L, Qian JQ, Qiu TZ, Shu YQ, Liu P. Temozolomide for treatment of brain metastases: A review of 21 clinical trials. World J Clin Oncol. 2014;5(1):19–27.
Yang LJ, Zhou CF, Lin ZX. Temozolomide and radiotherapy for newly diagnosed glioblastoma multiforme: a systematic review. Cancer Invest. 2014;32(2):31–6.
Kelsey CR, Beaven AW, Diehl LF, Prosnitz LR. Combined-modality therapy for early-stage Hodgkin lymphoma: maintaining high cure rates while minimizing risks. Oncology (Williston Park). 2012;26(12):1182–9. 1193.
Nicholson S, Hall E, Harland SJ, Chester JD, Pickering L, Barber J, Elliott T, Thomson A, Burnett S, Cruickshank C, et al. Phase II trial of docetaxel, cisplatin and 5FU chemotherapy in locally advanced and metastatic penis cancer (CRUK/09/001). British J Cancer. 2013;109(10):2554–9.
Curtin NJ, Szabo C. Therapeutic applications of PARP inhibitors: Anticancer therapy and beyond. Mol Aspects Med. 2013;34(6):1217–56.
Papeo G, Casale E, Montagnoli A, Cirla A. PARP inhibitors in cancer therapy: an update. Expert Opin Ther Pat. 2013;23(4):503–14.
Pommier Y. Drugging topoisomerases: lessons and challenges. ACS Chem Biol. 2013;8(1):82–95.
Singh P, Alex JM, Bast F. Insulin receptor (IR) and insulin-like growth factor receptor 1 (IGF-1R) signaling systems: novel treatment strategies for cancer. Med Oncol. 2014;31(1):805.
Grimaldi AM, Guida T, D'Attino R, Perrotta E, Otero M, Masala A, Carteni G. Sunitinib: bridging present and future cancer treatment. Ann Oncol. 2007;18 Suppl 6:vi31–4.
Ye F, Gao Q, Cai MJ. Therapeutic targeting of EGFR in malignant gliomas. Expert Opin Ther Targets. 2010;14(3):303–16.
Husseinzadeh N, Husseinzadeh HD. mTOR inhibitors and their clinical application in cervical, endometrial and ovarian cancers: a critical review. Gynecol Oncol. 2014;133(2):375–81.
Li J, Kim SG, Blenis J. Rapamycin: one drug, many effects. Cell Metab. 2014;19(3):373–9.
Pitts TM, Davis SL, Eckhardt SG, Bradshaw-Pierce EL. Targeting nuclear kinases in cancer: development of cell cycle kinase inhibitors. Pharmacol Ther. 2014;142(2):258–69.
Mikstacka R, Stefanski T, Rozanski J. Tubulin-interactive stilbene derivatives as anticancer agents. Cell Mol Biol Lett. 2013;18(3):368–97.
Fox E, Mosse YP, Meany HM, Gurney JG, Khanna G, Jackson HA, Gordon G, Shusterman S, Park JR, Cohn SL, et al. Time to disease progression in children with relapsed or refractory neuroblastoma treated with ABT-751: A report from the Children's Oncology Group (ANBL0621). Pediatr Blood Cancer. 2013.
Rohena CC, Mooberry SL. Recent progress with microtubule stabilizers: new compounds, binding modes and cellular activities. Nat Prod Rep. 2014;31(3):335–55.
Rosenberg B. Platinum coordination complexes in cancer chemotherapy. Naturwissenschaften. 1973;60(9):399–406.
Shen DW, Pouliot LM, Hall MD, Gottesman MM. Cisplatin resistance: a cellular self-defense mechanism resulting from multiple epigenetic and genetic changes. Pharmacol Rev. 2012;64(3):706–21.
Schilling T, Keppler KB, Heim ME, Niebch G, Dietzfelbinger H, Rastetter J, Hanauske AR. Clinical phase I and pharmacokinetic trial of the new titanium complex budotitane. Invest New Drugs. 1996;13(4):327–32.
Lümmen G, Sperling H, Luboldt H, Otto T, Rübben H. Phase II trial of titanocene dichloride in advanced renal-cell carcinoma. Cancer Chemother Pharmacol. 1998;42(5):415–7.
Korfel A, Scheulen ME, Schmoll HJ, Grundel O, Harstrick A, Knoche M, Fels LM, Skorzec M, Bach F, Baumgart J, et al. Phase I clinical and pharmacokinetic study of titanocene dichloride in adults with advanced solid tumors. Clin Cancer Res. 1998;4(11):2701–8.
Mross K, Robben-Bathe P, Edler L, Baumgart J, Berdel WE, Fiebig H, Unger C. Phase I Clinical Trial of a Day-1, −3, −5 Every 3 WeeksPhase I Clinical Trial of Day-1, −3, −5 Every 3 Weeks Schedule with Titanocene Dichloride (MKT 5) in Patients with Advanced Cancer. (Phase I Study Group of the AIO of the German Cancer Society). Onkologie. 2000;23(6):576–9.
Toney JH, Marks TJ. Hydrolysis chemistry of the metallocene dichlorides M (. eta. 5-C5H5) 2Cl2, M = titanium, vanadium, or zirconium. Aqueous kinetics, equilibria, and mechanistic implications for a new class of antitumor agents. J Am Chem Soc. 1985;107(4):947–53.
Immel TA, Debiak M, Groth U, Bürkle A, Huhn T. Highly selective apoptotic cell death induced by halo-salane titanium complexes. Chem Med Chem. 2009;4(5):738–41.
Manna CM, Braitbard O, Weiss E, Hochman J, Tshuva EY. Cytotoxic salan-titanium(IV) complexes: high activity toward a range of sensitive and drug-resistant cell lines, and mechanistic insights. Chem Med Chem. 2012;7(4):703–8.
Immel TA, Groth U, Huhn T, Ohlschlager P. Titanium salan complexes displays strong antitumor properties in vitro and in vivo in mice. PLoS One. 2011;6(3), e17869.
Köpf-Maier P. Intracellular localization of titanium within xenografted sensitive human tumors after treatment with the antitumor agent titanocene dichloride. J Struct Biol. 1990;105(1–3):35–45.
Harding MM, Mokdsi G. Antitumour metallocenes: structure-activity studies and interactions with biomolecules. Curr Med Chem. 2000;7(12):1289–303.
Balsells J, Carroll PJ, Walsh PJ. Achiral tetrahydrosalen ligands for the synthesis of C(2)-symmetric titanium complexes: a structure and diastereoselectivity study. Inorg Chem. 2001;40(22):5568–74.
Immel TA, Groth U, Huhn T. Cytotoxic titanium salan complexes: surprising interaction of salan and alkoxy ligands. Chemistry. 2010;16(9):2775–89.
Moreno-Villanueva M, Pfeiffer R, Sindlinger T, Leake A, Muller M, Kirkwood TB, Burkle A. A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaks. BMC Biotechnol. 2009;9:39.
Debiak M, Panas A, Steinritz D, Kehe K, Burkle A. High-throughput analysis of DNA interstrand crosslinks in human peripheral blood mononuclear cells by automated reverse FADU assay. Toxicology. 2011;280(1–2):53–60.
Chou TC, Talalay P. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Regul. 1984;22:27–55.
Narita M, Nunez S, Heard E, Lin AW, Hearn SA, Spector DL, Hannon GJ, Lowe SW. Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence. Cell. 2003;113(6):703–16.
Lawless C, Wang C, Jurk D, Merz A, Zglinicki T, Passos JF. Quantitative assessment of markers for cell senescence. Exp Gerontol. 2010;45(10):772–8.
Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci U S A. 1995;92(20):9363–7.
Wyrsch P, Blenn C, Pesch T, Beneke S, Althaus FR. Cytosolic Ca2+ shifts as early markers of cytotoxicity. Cell Commun Signal. 2013;11(1):11.
Lazebnik YA, Kaufmann SH, Desnoyers S, Poirier GG, Earnshaw WC. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature. 1994;371(6495):346–7.
Kaufmann SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirier GG. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res. 1993;53(17):3976–85.
Shen C, Gu M, Song C, Miao L, Hu L, Liang D, Zheng C. The tumorigenicity diversification in human embryonic kidney 293 cell line cultured in vitro. Biologicals. 2008;36(4):263–8.
Schur J, Manna CM, Tshuva A, Ott I. Quantification of the titanium content in metallodrugexposed tumor cells using HR-CS AAS. Metallodrugs. 2014;1:1–9.
Bürkle A, Virag L. Poly(ADP-ribose): PARadigms and PARadoxes. Mol Asp Med. 2013;34(6):1046–65.
Firsanov DV, Solovjeva LV, Svetlova MP. H2AX phosphorylation at the sites of DNA double-strand breaks in cultivated mammalian cells and tissues. Clin Epigenetics. 2011;2(2):283–97.
Paoletti A, Giocanti N, Favaudon V, Bornens M. Pulse treatment of interphasic HeLa cells with nanomolar doses of docetaxel affects centrosome organization and leads to catastrophic exit of mitosis. J Cell Sci. 1997;110(Pt 19):2403–15.
Yang H, Ganguly A, Cabral F. Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs. J Biol Chem. 2010;285(42):32242–50.
Coulthard LR, White DE, Jones DL, McDermott MF, Burchill SA. p38(MAPK): stress responses from molecular mechanisms to therapeutics. Trends Mol Med. 2009;15(8):369–79.
Lamkanfi M, Kanneganti TD. Caspase-7: a protease involved in apoptosis and inflammation. Int J Biochem Cell Biol. 2010;42(1):21–4.
Fiset PO, Wou K, Arseneau J, Gilbert L. Vulvar Carcinosarcoma Secondary to Radiotherapy: A Case Report and Review of the Literature. J Low Genit Tract Dis. 2014.
Kawamitsu H, Hoshino H, Okada H, Miwa M, Momoi H, Sugimura T. Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures. Biochemistry. 1984;23(16):3771–7.
We wish to thank Prof. Felix Althaus for stimulating discussions regarding the manuscript and his support.
PW was supported by Lotte and Adolf Hotz-Sprenger Foundation, TI was supported by Konstanz Research School of Chemical Biology.
Availability of data and materials
The datasets supporting the conclusions of this article are available in the Zenodo repository, https://zenodo.org/record/51689.
TP carried out most of the western blotting experiments, HS carried out and analyzed the FADU experiments and revised the manuscript. PW carried out and analyzed the calcium-toxicity assay and revised the manuscript, TI synthesized titanium(IV)salan complexes, WD was responsible for genotyping and revised the manuscript. TH supervised TI, participated in study design and revised the manuscript, AB supervised HS, participated in study design and revised the manuscript. SB designed the study, supervised TP and HS, drafted the manuscript, carried out and analyzed the immunofluorescence experiments, the viability assays, the flow cytometry and senescence analysis and some western blots, including all statistical evaluations. All authors read and approved the final manuscript.
The authors declare that they have no competing interest.
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Structure of Tc52 and Tc53. Top structure highlights the identical backbone of Tc52 and Tc53. R (circled in red) indicates the two different side-chains of Tc52 (methyl-group) and Tc53 (tert-butyl-group), respectively. Depicted below are the separate structures for Tc52 and Tc53. (PDF 1085 kb)
Cell viability assay with HeLa tumor cells (squares) and VH7 normal fibroblasts (circles). Cells were exposed to increasing concentrations of Tc52 (filled) or Tc53 (open) titanium(IV)salan compounds solubilized in DMSO and incubated for 48 h. Subsequently, medium was replaced by fresh medium containing 9 μg/ml resazurin and cells were further incubated. Viability is expressed as % of solvent control. Whereas Tc53 is non-toxic in the tested concentration range, Tc52 impairs viability in HeLa and VH7 cells with an EC20 of 3 μM and 1 μM, an EC50 of 6 μM and 3 μM, respectively, and an EC80 of about 10 μM for both. (PDF 1085 kb)
Detection of DNA strand break markers poly(ADP-ribosyl)ation and γH2AX in HeLa and VH7. A: PAR can be weakly detected in HeLa cells about 5 min after application of 500 μM H2O2 in cell culture medium, peaking at 10 min, and signals disappear completely after 60 min. Later time points are not depicted. Phosphorylated H2AX appears after 10 min incubation as a pan-nuclear signal, with pronounced characteristic foci formation after 6 h. B: PAR can be weakly detected in VH7 cells about 5 min after application of 500 μM H2O2 in cell culture medium, peaking at 30 min and signals disappear completely after 60 min. Later time points are not depicted. Phosphorylated H2AX appears after 10 min incubation as a pan-nuclear signal, with less well pronounced characteristic foci formation after 60 min. C: H2AX and-tubulin detection in a time-course from 0 min (Ctr) to 1440 min (24 h) after application of 500 μM H2O2 (H), 10 μM Tc52 (52) or 10 μM Tc53 (53) to HeLa cells. Signal-ratio evaluation of γH2AX/tubulin is presented in the lower panel. γH2AX formation in H2O2 treated samples is evident after 30 min and increases over time. Only after 24 h of Tc52 treatment, a mild increase in γH2AX is detectable, concurrent with the onset of cell death (Fig. 1a). (PDF 1085 kb)
Cell cycle distribution profile of HeLa cells after continuous Tc52 treatment. Representative histograms from data presented in Fig. 2. A: Cell-cycle distribution after 30 h of continuous treatment of cells with 1-10 μM Tc52 (concentration range showing an effect). Note the small (not significant) reduction in G1 with 2 μM Tc52 and the strong reduction with 5 μM and 10 μM Tc52 concomitant with a substantial increase in the subG1 fraction. The sample treated with 5 μM Tc52 displays in addition an increased number of cells in G2, whereas cells treated with 10 μM show a reduction in G2 phase. B: Cell-cycle distribution after 48 h of continuous treatment of cells with 1-10 μM Tc52 (concentration range showing an effect). Note the reduction in G1 with 1-2 μM Tc52 and the nearly complete loss in samples treated with 5 μM and 10 μM Tc52. SubG1 is increased in all samples and cells treated with 1 μM Tc52 display a significant increase in G2 phase. (PDF 1085 kb)
Cell cycle distribution profile of HeLa cells after treatment with M-phase targeting toxins in combination with Tc52. Representative flow-cytometry histograms from data presented in Fig. 3. For better visibility, only one M-phase targeting toxin with or without 6 μM Tc52 is displayed in each panel. There is no significant difference in cell-cycle profiles of Tc52 or control samples except a small increase G2-phase in Tc52 treated cells (upper left panel). CytB-treated samples show a near-complete loss of G1-peak and a reduction in G2, concomitant with an increase in subG1 and the appearance of a substantial cell-fraction with a >4 N DNA content with a strong peak at about 8 N (compare black graphs from upper right and upper left panel). Combination with Tc52 reduces this 8 N peak and increases the number of cells in G2 and slightly the number of cells in G1 (upper right panel). Col-treatment induces a reduction in G1-phase (compare the black graphs from lower left and upper left panel) concomitant with an increase in G2 and subG1 (lower left panel). Combination with Tc52 does not change the cell-cycle profile significantly. 50 nM Doc induces a complete loss of cells from G1, concomitant with an increase in subG1 fraction, number of cells in G2 as wells as cells with a > 4 N DNA content (compare black graphs from lower right panel with upper left panel). Addition of Tc52 decreased the number of cells with a > 4 N DNA content (lower right panel). (PDF 1085 kb)
Cell cycle distribution profile of cells after 6 h of treatment. Representative flow-cytometry histograms from data presented in Fig. 4. For better visibility, surface of the graph from Ctr sample (black line) is dotted. A: Cell-cycle distribution of HeLa cells after 6 h incubation with Titanium(IV)salan complexes Tc52 and Tc53 30 h after treatment start. Only 10 μM Tc52 shows significant impact on cell-cycle profile, i.e. severe reduction in G1 and increase of the subG1 fraction. B: Cell-cycle distribution of VH7 normal fibroblasts after 6 h incubation with Titanium(IV)salan complexes Tc52 and Tc53 30 h after treatment start. Samples exposed to 5 μM and 10 μM Tc52 display major reduction in number of cells in G1 concomitant with a mild increase in G2-phase. SubG1 fraction is not significantly elevated. (PDF 1086 kb)
Analysis of rapid free cytosolic Ca2 + -shifts in HeLa tumor cells and VH7 normal fibroblasts. Cells were processed as suggested by the manufacturer. Tc52 or Tc53 or solvent control (CTR) was administered to the cells and Ca2 + -dependent increase in fluorescence of Fluo4-NW-dye was measured. Significant increase in fluorescence is an indicator for cell death signaling. A: Increase in free cytosolic Ca2+ in HeLa cells. Both concentrations show significant and rapid increase in Ca2+ signal, reaching after 5 sec 4 and 5.5 RFU above CTR and after 10 sec 6.5 and 5 RFU above CTR, whereas control and Tc53 values stay low. B: No significant increase in free cytosolic Ca2+ in VH7 cells. Only the 10 μM Tc52 exposure samples display initially a mild increase (1 RFU above CTR), which is lost after 10 sec (0 RFU above CTR). As summary, rapid Ca2+ increase is indicative for cytotoxicity of Tc52 in HeLa cells, whereas its toxicity is negligible in VH7 normal fibroblasts, in line with other data. (PDF 1085 kb)
Additional Methods. (PDF 144 kb)
Analysis of p38 kinase and caspase activation by Tc53 in HeLa and VH7 cells. Cells were exposed to 10 μM Tc53 or solvent for 30 h and subsequently lysed in Laemmli buffer. Western blot analysis was performed as described for Figs. 6 and 7. There is no evidence for activation of p38 in. HeLa or VH7 cells (A) or cleavage of caspase7 (B). Significance was tested using two-tailed T-test. Panel (C) depicts the respective western blot for cleaved caspase7 (cleaved Casp7), GAPDH, total p38 stresskinase (p38), phosphorylated p38 (p-p38) and αtubulin (Tubulin). +: positive control, C: solvent control samples, T: 10 μM Tc53 treated samples. Numbers indicate respective independent experiments. (PDF 1085 kb)
Cell viability assay with U2OS tumor cells (squares) and low-passage HEK293 (circles). Cells were exposed to increasing concentrations of Tc52 (filled) or Tc53 (open) titanium(IV)salan compounds solubilized in DMSO and incubated for 48h. Subsequently, medium was replaced by fresh medium containing 9 μg/ml resazurin and cells were further incubated. Viability is expressed as % of solvent control. Whereas Tc53 is non-toxic in the tested concentration range, Tc52 impairs viability in U2OS and HEK293 cells with an EC20 of 1.5 μM and 2.5 μM, an EC50 of 2 μM and 4 μM, and an EC80 of about 4 μM and 20 μM, respectively. (PDF 1085 kb)
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Pesch, T., Schuhwerk, H., Wyrsch, P. et al. Differential cytotoxicity induced by the Titanium(IV)Salan complex Tc52 in G2-phase independent of DNA damage. BMC Cancer 16, 469 (2016). https://doi.org/10.1186/s12885-016-2538-0
- Titanium(IV)salan complex