Fig. 2

Dose-dependent changes in cell-cycle profile by Tc52. a: Cell-cycle distribution of HeLa cells after 30 h incubation with increasing concentrations of Tc52. Filled bars: G1; open bars: S; hatched bars: G2/M; vertical line bars: subG1. Dose-dependent increase (30-fold) in subG1 from 1 μM to 10 μM with significant decrease in G1 starting at 5 μM (2-fold), the latter accompanied by accumulation in G2/M (1.5-fold). Tc53 has no impact on cell-cycle distribution. b: Cell-cycle distribution of HeLa cells after 48 h incubation with increasing concentrations of Tc52. Filled bars: G1; Pointed bars: S; Hatched bars: G2/M; Open bars: subG1. Dose-dependent increase (44-fold) in subG1 from 1 μM to 10 μM concomitant with significant decrease in G1 (2.7-fold), accompanied by fluctual accumulation in G2/M. Tc53 has no impact on cell-cycle distribution. Treatments were compared to controls and significance was calculated by two-way ANOVA with Dunnett's Multiple Comparison Test. p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***