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Genetic variability in CYP3A4 and CYP3A5in primary liver, gastric and colorectal cancer patients

BMC Cancer volume 7, Article number: 118 (2007) Cite this article

Abstract

Background

Drug-metabolizing enzymes play a role in chemical carcinogenesis through enzymatic activation of procarcinogens to biologically reactive metabolites. The role of gene polymorphisms of several cytochrome P450 enzymes in digestive cancer risk has been extensively investigated. However, the drug-metabolizing enzymes with the broader substrate specificity, CYP3A4 and CYP3A5, have not been analyzed so far. This study aims to examine associations between common CYP3A4 and CYP3A5 polymorphisms and digestive cancer risk.

Methods

CYP3A4 and CYP3A5 genotypes were determined in 574 individuals including 178 patients with primary liver cancer, 82 patients with gastric cancer, 151 patients with colorectal cancer, and 163 healthy individuals.

Results

The variant allele frequencies for patients with liver cancer, gastric cancer, colorectal cancer and healthy controls, respectively, were: CYP3A4*1B, 4.8 % (95% C.I. 2.6–7.0), 3.7 % (0.8–6.6) 4.3% (2.0–6.6) and 4.3% (2.1–6.5); CYP3A5*3, 91.8 % (93.0–97.4), 95.7% (92.6–98.8), 91.7% (88.6–94.8) and 90.8% (87.7–93.9). The association between CYP3A4*1B and CYP3A5*3 variant alleles did not significantly differ among patients and controls. No differences in genotypes, allele frequencies, or association between variant alleles were observed with regard to gender, age at diagnosis, tumour site or stage.

Conclusion

Common polymorphisms on CYP3A4 and CYP3A5 genes do not modify the risk of developing digestive cancers in Western Europe.

Peer Review reports

Background

The identification of low penetrance genes able to increase the risk of developing cancer could constitute a major tool for the identification of individuals with inheritable altered susceptibility. In this regard, the role of drug-metabolizing enzymes in cancer risk has been the object of several hundreds of studies performed over the last decade [1]. The main hypothesis underlying the link between drug-metabolizing enzymes and chemical carcinogenesis is based on the enzymatic activation of procarcinogens to biologically reactive metabolites. These reactive metabolites would interact with DNA, thereby causing altered gene expression or function, and eventually carcinogenesis. The primary metabolism of a variety of xenobiotic carcinogens is mainly mediated by cytochrome P450 (CYP) enzymes belonging to the CYP 1, 2 or 3 families, which together comprise 25 different isoenzymes. Among them, the most relevant are CYP1A1, CYP1A2, CYP1B1, CYP2E1, CYP3A4 and CYP3A5 [2–4]. Most of the latter enzymes are polymorphic, mutated alleles causing abolished, reduced or altered enzyme activity. To date, several studies have focused on the role of gene polymorphisms or enzyme activities of CYP1A1 [5–11], CYP1A2 [7, 12–14], CYP1B1 [7, 15] and CYP2E1 [6, 16–19] in digestive cancer risk. Nevertheless, the impact on cancer risk of polymorphisms of the CYP enzymes with the broader substrate specificity, namely CYP3A4 and CYP3A5, has not been analyzed in detail so far.

CYP3A4 and CYP3A5 enzymes are the major enzymes for drug metabolism in adults [20], both enzymes making up nearly 30% of the total CYP enzymes expressed in the human liver [21]. Since no individualized quantification of CYP3A4 and CYP3A5 in vivo is presently possible due to a wide substrate specificity overlap, the joined enzyme activities are designated as CYP3A [22]. Both CYP3A4 and CYP3A5 are expressed in liver, stomach, colorectal epithelium and in colorectal cancer tissue [23–26], although large interindividual differences exist in the expression of both enzymes [27–29]. Besides a local effect of the enzyme activities in the metabolism of carcinogens, CYP3A4/5-dependent metabolism in the proximal intestine is likely to affect the luminal or vascular delivery of carcinogenic molecules to the liver or the colon.

These facts provide a plausible hypothesis for organ-specific dietary procarcinogen activation and therefore to digestive cancer risk. The hypothesis that justify the present study is based in the fact that CYP3A4 and CYP3A5 enzyme activities are involved in the activation of several procarcinogens, including aflatoxin B1 stergmatocystin, food-derived heterocyclic amines, alpha-hydroxytamoxifen and N'-nitrosonornicotine, and that both enzymes are expressed to a high level in the digestive tract [27, 30–36]. Thus, it can be hypothesized that genetic differences causing variability in the regulation, expression or activity of CYP3A enzymes would be relevant factors modifying cancer susceptibility or clinical outcome. These allele-disease associations have already been shown for CYP3A gene polymorphisms and prostate, breast and lung cancer [37–43], albeit with controversial results. Although CYP3A4 and CYP3A5 play a relevant biological role in liver and in gut epithelium, no studies have addressed whether polymorphism of these enzymes are related to digestive cancer risk, probably because variant alleles for the corresponding genes have just recently been described. In this regard, novel findings show that CYP3A5 genotypes leading to high enzyme activity are related to oesophageal cancer [44]. However the interaction of CYP3A4 and CYP3A5 gene polymorphisms and the risk of developing the major digestive cancers such as liver, stomach or colorectal cancers remain unexplored. This study aims to analyze such interaction.

Methods

The study group consisted of 411 unrelated patients with digestive cancers, including 178 patients with primary liver cancer, 82 patients with gastric cancer and 151 patients with colorectal cancer, and 163 healthy subjects. Table I shows a summary of the study groups included in the study. All the participants were white Spanish individuals, living in the same areas as the patients (Madrid and surrounding areas), and were included in the study after giving informed written consent. All the patients diagnosed with liver, stomach or colorectal cancer that attended the collaborating Services of the selected Hospitals were included in the study. The diagnosis was based on histology analyses of endoscopic biopsies and/or surgical resection specimens. Data regarding known previous digestive diseases, alcohol and tobacco consumption, serum tests for hepatitis B and C virus and other diseases were collected. Heavy drinkers were defined as individuals drinking more than 50 g of alcohol per day.

Table 1 Summary of the study groups.
Full size table

All the patients were requested to participate in the study, and all of them agreed to do so. Control samples were obtained from medical students, University and Hospital staff. A medical examination was made to identify subjects in good health. Over 95% of the healthy subjects requested agreed to participate in the study. The protocol was approved by the Ethics Committee of the San Carlos University Hospital, Madrid. A possible confounding factor in the present study is that, within a study group, the frequency of individuals carrying a determined variant allele may change with age in the event that the presence of such allele would be related to severe diseases. If a determined genotype has a "protecting effect" against any disease, it may be expected that in populations composed of older subjects there is an increased frequency of such a protecting genotype. Therefore we included within the control group a selected subgroup of 41 healthy subjects with ages ranging from 90 to 95 years [45]. The analysis of the CYP3A4 and CYP3A5 genotype indicates frequencies for genotypes that were identical to those of younger healthy subjects. The frequencies for CYP3A4*1B and CYP3A5*3 alleles were similar for younger and older controls (see Results section). 4.33% and 90.7% for younger controls and 4.18 and 91.2% for older controls. Another possible confounder is related to the fact that control subjects are highly educated people, and differences in lifestyle as compared to cancer patients may be expected. Since digestive cancers are partly related to diet, these changes in lifestyle may be relevant. However, it should be stated that patients and controls were interviewed to assure that diet and lifestyle do no differ between patients and control subjects.

Blood samples from all participants were stored at -80°C until analysis. The genomic DNA was prepared from peripheral leucocytes, and dissolved in sterile 10 mM Tris-HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid, at a final concentration of 400 to 600 mg per ml. DNA samples were purified according standard procedures [46] and stored at 4°C in sterile plastic vials.

The analyses for the CYP3A4*1B and CYP3A5*3 gene variants were carried out by amplification-restriction procedures as described elsewhere [47, 48]. These allelic variants were analyzed instead of others because these are common variants that cause functional changes. [47, 48].

Statistical analysis

The study was designed taking into consideration the expected frequency of individuals expressing CYP3A5, i.e. carrying at least one CYP3A5*1 allele, which represents 13% of the Caucasian population [20]. The design permits the identification as statistically significant of a 1.7-fold increase or a 2-fold decrease in the frequency for CYP3A5 expressers among cancer patients. Statistical power was evaluated with a genetic model to analyze the frequency for carriers of the disease gene with an RR value = 2.5 (α = 0.05). The power calculated for associations with cancer risk for the presence of the CYP3A4*1B and CYP3A5*3 alleles is 90.2% and 99.3% for liver cancer, 74.5% and 93.9% for gastric cancer and 87.7% and 98.8% for colorectal cancer, respectively. The intergroup comparison values were calculated by using the statistical package SPSS 11.0.1. (SPSS Inc. Chicago, Ill, USA). The Chi-square (X2) test was used for comparison of genotype frequencies and association of CYP3A4 and CYP3A5 variant alleles, unless the conditions for the application of this test were not adequate. In such cases, Fisher's exact test was used to calculate the p value.

Results

Seven different combinations of CYP3A4 and CYP3A5 genotypes were identified in the study groups. The summary of the genotypes is shown in Table 2. The comparison of genotype frequencies among digestive cancer patients and healthy controls indicates a similar genotype frequency across the study groups. No statistically significant differences were observed. The variant allele frequencies, as calculated from genotypes shown in Table 2, were almost identical for all study groups: CYP3A4*1B was present with allele frequencies of 4.8 (95% CI 2.6–6.9), 3.7 (95% CI 0.8–6.5) and 4.3% (95% CI 2.0–6.6) for liver, stomach and colorectal cancer patients, respectively, and 4.3% (2.1–6.5) for healthy individuals. The allele frequencies for CYP3A5*3 were 91.9 (95% CI 89.0–94.7), 95.7 (95% CI 92.6–98.8), and 91.7% (95% CI 88.6–94.8) for liver, stomach and colorectal cancer patients, respectively, and 90.8% (95% CI 87.7–93.9) among healthy individuals. No statistically significant differences in the allele frequencies were observed, although the frequency for the functional CYP3A5*1 allele among stomach cancer patients is lower (approximately 50%) than those observed for the rest of the study groups (p = 0.051 as compared to healthy subjects).

Table 2 CYP3A4 and CYP3A5 genotypes in digestive cancer patients and healthy control individuals.
Full size table

Both CYP3A4 and CYP3A5 genotypes were at Hardy-Weinberg's equilibrium among cases and controls and variant allele frequencies are consistent with those reported for both, European and American Caucasians [20, 49]. Regarding the association between the CYP3A4*1B and CYP3A5*1 alleles, the proportion of individuals carrying both variants is much higher for all the study groups (6.7%, 6.1% and 4.6% for liver, gastric or colorectal cancer patients and 7.4% for control subjects) than expected from a random association of these alleles (0.3 to 0.8% of individuals, as calculated from actual allele frequencies in the study groups). These findings are in agreement with a previous study that reports such an SNP association in another Caucasian population with a frequency of 7.1%. [22, 43]. Among patients, age at diagnosis was similar in all subgroups of patients, and this parameter was not related to the genotypes. Regarding control subjects, the frequencies for CYP3A4*1B and CYP3A5*3 alleles were 4.33% and 90.7% for younger controls and 4.18 and 91.2% for older controls. Gender, previous surgical therapy or chemotherapy, alcohol abuse or tobacco use did not influence the distribution of the polymorphisms studied among patients. Among liver cancer patients no differences in the distribution of polymorphisms regarding chronic infection with hepatitis B or C virus were observed. Among gastric cancer patients no statistically significant differences were observed regarding the anatomical site of the tumour. In contrast, significant differences showing an increase in the frequency of carriers of the CYP3A4*1A plus CYP3A5*3 haplotype, were observed among patients with intestinal-type gastric cancer as compared to healthy controls (95.3% versus 82.8%, p < 0.05).

Colorectal cancer patients were divided into three subgroups according to the anatomical site of the tumour (non-sigmoid colon, sigmoid colon and rectum), because differences in the association of other polymorphisms of drug-metabolizing enzymes related to tumour site have been reported [50, 51]. The results are summarized in Table 3. An excess of patients with non-sigmoid colon cancer carrying the combined genotype CYP3A4*1A/*1B plus CYP3A5*3/*3 was observed as compared to healthy subjects (Chi-square = 10.3, p = 0.0015, non significant in multiple comparison analyses) With regard to allele frequencies, CYP3A4*1B allele frequency was 6.6%, 5.9% and 1% and CYP3A5*3 frequency was 91.1%, 90.1% and 93.6% among patients with non-sigmoid colon, sigmoid colon and rectum, respectively. The proportion of individuals carrying CYP3A4*1B and CYP3A5*1 alleles was 13.3%, 9.8% and 1.8% for patients with non-sigmoid colon, sigmoid colon and rectum, respectively. None of these differences were statistically significant. No differences regarding Dukes' stage of the tumour were observed.

Table 3 CYP3A4 and CYP3A5 genotypes in colorectal cancer patients according tumor site.
Full size table

Discussion

Metabolism related to CYP enzymes differ between organs and tissues, and therefore it is expected that tissue specificity towards cancer-inducing substances is related to site-specific expression of xenobiotic-metabolizing enzymes. Digestive cancers are strongly related to dietary factors, and mechanisms involving the interaction of such dietary factors with determined polymorphisms of xenobiotic-metabolizing enzymes have been proposed [10]. Most of the CYP genes expressed in human gut and liver, namely CYP1A1, CYP1A2, CYP1B1, CYP3A4 and CYP3A5 [25], are of great relevance for carcinogen activation. With the exception of CYP3A4 and CYP3A5, all genes coding for these enzymes have been studied with regard to digestive cancer risk [1]. This study was aimed to fill the gap in the present knowledge of the putative role of polymorphisms of the two remaining relevant CYP enzymes in the digestive tract.

CYP3A enzyme activity shows interindividual variability due to the combined effect of genetics and interaction with drugs or environmental chemicals [22]. Both CYP3A4 and CYP3A5 genes are polymorphic and several variant alleles have been described for either the CYP3A4 or the CYP3A5 gene (for an updated list of alleles, see [52]). Only two variant alleles that are at linkage disequilibrium, namely CYP3A4*1B and CYP3A5*3 are common across diverse ethnic populations and have functional relevance. Other variant alleles are either extremely rare or irrelevant for enzyme activity [20], and therefore they were not analyzed in this study.

Present evidences indicate that CYP3A4*1B and CYP3A5*3 are functional polymorphisms in vivo. Regarding CYP3A4*1B, it seems to modify the ability to metabolize some CYP3A substrates, such as quinine [53], although it does not influence the metabolism of other substrates such as midazolam or dextromethorphan [49, 54]. CYP3A5*3 is the commonest CYP3A5 allele and is associated with severely decreased enzyme activity [20].

The present study provides novel findings on the putative role of CYP3A4 and CYP3A5 polymorphisms in digestive cancer risk. In spite of the presence of CYP3A enzyme activities in gut and liver, and of the relevant role of such enzyme activities in carcinogen activation, our results do not support a major link between common CYP3A4 and CYP3A5 polymorphisms and digestive cancer risk. Nevertheless the possibility for a low to moderate effect for these polymorphisms especially in the smallest group, stomach cancer, can not be ruled out. Minor differences in the frequencies for individuals carrying the CYP3A4*1A plus CYP3A5*3 haplotypes were observed in some subgroups of gastric or colon cancer, as compared to healthy subjects, with p values under 0.05. These differences cannot be considered statistically significant in multiple-comparison analyses, and do not seem to have a relevant clinical impact. However, this is a topic that deserves investigation and further studies should focus on the role of such polymorphisms in liver, gastric or colorectal cancer in populations with extreme incidence rates of these cancers.

Conclusion

Taking together the findings reported in the present study, it can be concluded that common polymorphisms of CYP3A enzymes with functional relevance are not significant factors in digestive carcinogenesis in Western Europe. This is in contrast to other human cancers that are related to such polymorphisms. Since human guts are the primary tissues that have contact with dietary carcinogens, it is intriguing that digestive cancer risk is not modified by genetic factors that are related to other human cancers such as prostate, breast or lung, thus reinforcing the hypothesis that the association of CYP3A polymorphisms with prostate or breast cancer are related to endogenous substances rather than xenobiotics carcinogens [37–43].

References

  1. Agundez JA: Cytochrome P450 gene polymorphism and cancer. Curr Drug Metab. 2004, 5: 211-224. 10.2174/1389200043335621.

    Article  CAS  PubMed  Google Scholar 

  2. Ingelman-Sundberg M: Polymorphism of cytochrome P450 and xenobiotic toxicity. Toxicology. 2002, 181-182: 447-452. 10.1016/S0300-483X(02)00492-4.

    Article  CAS  PubMed  Google Scholar 

  3. Kamataki T, Fujita K, Nakayama K, Yamazaki Y, Miyamoto M, Ariyoshi N: Role of human cytochrome P450 (CYP) in the metabolic activation of nitrosamine derivatives: application of genetically engineered Salmonella expressing human CYP. Drug Metab Rev. 2002, 34: 667-676. 10.1081/DMR-120005668.

    Article  CAS  PubMed  Google Scholar 

  4. Williams JA: Single nucleotide polymorphisms, metabolic activation and environmental carcinogenesis: why molecular epidemiologists should think about enzyme expression. Carcinogenesis. 2001, 22: 209-214. 10.1093/carcin/22.2.209.

    Article  CAS  PubMed  Google Scholar 

  5. Ye Z, Parry JM: Genetic polymorphisms in the cytochrome P450 1A1, glutathione S-transferase M1 and T1, and susceptibility to colon cancer. Teratog Carcinog Mutagen. 2002, 22: 385-392. 10.1002/tcm.10035.

    Article  CAS  PubMed  Google Scholar 

  6. Kiss I, Sandor J, Pajkos G, Bogner B, Hegedus G, Ember I: Colorectal cancer risk in relation to genetic polymorphism of cytochrome P450 1A1, 2E1, and glutathione-S-transferase M1 enzymes. Anticancer Res. 2000, 20: 519-522.

    CAS  PubMed  Google Scholar 

  7. Sachse C, Smith G, Wilkie MJ, Barrett JH, Waxman R, Sullivan F, Forman D, Bishop DT, Wolf CR: A pharmacogenetic study to investigate the role of dietary carcinogens in the etiology of colorectal cancer. Carcinogenesis. 2002, 23: 1839-1849. 10.1093/carcin/23.11.1839.

    Article  CAS  PubMed  Google Scholar 

  8. Chen K, Jiang QT, He HQ: Relationship between metabolic enzyme polymorphism and colorectal cancer. World J Gastroenterol. 2005, 11: 331-335.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Slattery ML, Samowtiz W, Ma K, Murtaugh M, Sweeney C, Levin TR, Neuhausen S: CYP1A1, cigarette smoking, and colon and rectal cancer. Am J Epidemiol. 2004, 160: 842-852. 10.1093/aje/kwh298.

    Article  PubMed  Google Scholar 

  10. Turner F, Smith G, Sachse C, Lightfoot T, Garner RC, Wolf CR, Forman D, Bishop DT, Barrett JH: Vegetable, fruit and meat consumption and potential risk modifying genes in relation to colorectal cancer. Int J Cancer. 2004, 112: 259-264. 10.1002/ijc.20404.

    Article  CAS  PubMed  Google Scholar 

  11. Roth MJ, Abnet CC, Johnson LL, Mark SD, Dong ZW, Taylor PR, Dawsey SM, Qiao YL: Polymorphic variation of Cyp1A1 is associated with the risk of gastric cardia cancer: a prospective case-cohort study of cytochrome P-450 1A1 and GST enzymes. Cancer Causes Control. 2004, 15: 1077-1083. 10.1007/s10552-004-2233-3.

    Article  PubMed  Google Scholar 

  12. Sachse C, Bhambra U, Smith G, Lightfoot TJ, Barrett JH, Scollay J, Garner RC, Boobis AR, Wolf CR, Gooderham NJ: Polymorphisms in the cytochrome P450 CYP1A2 gene (CYP1A2) in colorectal cancer patients and controls: allele frequencies, linkage disequilibrium and influence on caffeine metabolism. Br J Clin Pharmacol. 2003, 55: 68-76. 10.1046/j.1365-2125.2003.01733.x.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Ishibe N, Sinha R, Hein DW, Kulldorff M, Strickland P, Fretland AJ, Chow WH, Kadlubar FF, Lang NP, Rothman N: Genetic polymorphisms in heterocyclic amine metabolism and risk of colorectal adenomas. Pharmacogenetics. 2002, 12: 145-150. 10.1097/00008571-200203000-00008.

    Article  CAS  PubMed  Google Scholar 

  14. Le Marchand L, Hankin JH, Wilkens LR, Pierce LM, Franke A, Kolonel LN, Seifried A, Custer LJ, Chang W, Lum-Jones A, Donlon T: Combined effects of well-done red meat, smoking, and rapid N-acetyltransferase 2 and CYP1A2 phenotypes in increasing colorectal cancer risk. Cancer Epidemiol Biomarkers Prev. 2001, 10: 1259-1266.

    CAS  PubMed  Google Scholar 

  15. Gibson P, Gill JH, Khan PA, Seargent JM, Martin SW, Batman PA, Griffith J, Bradley C, Double JA, Bibby MC, Loadman PM: Cytochrome P450 1B1 (CYP1B1) is overexpressed in human colon adenocarcinomas relative to normal colon: implications for drug development. Mol Cancer Ther. 2003, 2: 527-534.

    CAS  PubMed  Google Scholar 

  16. Le Marchand L, Donlon T, Seifried A, Wilkens LR: Red meat intake, CYP2E1 genetic polymorphisms, and colorectal cancer risk. Cancer Epidemiol Biomarkers Prev. 2002, 11: 1019-1024.

    PubMed  Google Scholar 

  17. Butler WJ, Ryan P, Roberts-Thomson IC: Metabolic genotypes and risk for colorectal cancer. J Gastroenterol Hepatol. 2001, 16: 631-635. 10.1046/j.1440-1746.2001.02501.x.

    Article  CAS  PubMed  Google Scholar 

  18. Cai L, Zheng ZL, Zhang ZF: Cytochrome p450 2E1 polymorphisms and the risk of gastric cardia cancer. World J Gastroenterol. 2005, 11: 1867-1871.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Park GT, Lee OY, Kwon SJ, Lee CG, Yoon BC, Hahm JS, Lee MH, Hoo Lee D, Kee CS, Sun HS: Analysis of CYP2E1 polymorphism for the determination of genetic susceptibility to gastric cancer in Koreans. J Gastroenterol Hepatol. 2003, 18: 1257-1263. 10.1046/j.1440-1746.2003.03167.x.

    Article  CAS  PubMed  Google Scholar 

  20. Xie HG, Wood AJ, Kim RB, Stein CM, Wilkinson GR: Genetic variability in CYP3A5 and its possible consequences. Pharmacogenomics. 2004, 5: 243-272. 10.1517/phgs.5.3.243.29833.

    Article  CAS  PubMed  Google Scholar 

  21. Shimada T, Yamazaki H, Mimura M, Inui Y, Guengerich FP: Interindividual variations in human liver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: studies with liver microsomes of 30 Japanese and 30 Caucasians. J Pharmacol Exp Ther. 1994, 270: 414-423.

    CAS  PubMed  Google Scholar 

  22. Wilkinson GR: Genetic variability in cytochrome P450 3A5 and in vivo cytochrome P450 3A activity: some answers but still questions. Clin Pharmacol Ther. 2004, 76: 99-103. 10.1016/j.clpt.2004.04.005.

    Article  CAS  PubMed  Google Scholar 

  23. Jurutka PW, Thompson PD, Whitfield GK, Eichhorst KR, Hall N, Dominguez CE, Hsieh JC, Haussler CA, Haussler MR: Molecular and functional comparison of 1,25-dihydroxyvitamin D(3) and the novel vitamin D receptor ligand, lithocholic acid, in activating transcription of cytochrome P450 3A4. J Cell Biochem. 2004, 94 (5): 917-943. 10.1002/jcb.20359.

    Article  Google Scholar 

  24. Pfrunder A, Gutmann H, Beglinger C, Drewe J: Gene expression of CYP3A4, ABC-transporters (MDR1 and MRP1-MRP5) and hPXR in three different human colon carcinoma cell lines. J Pharm Pharmacol. 2003, 55: 59-66.

    Article  CAS  PubMed  Google Scholar 

  25. Ding X, Kaminsky LS: Human extrahepatic cytochromes P450: function in xenobiotic metabolism and tissue-selective chemical toxicity in the respiratory and gastrointestinal tracts. Annu Rev Pharmacol Toxicol. 2003, 43: 149-173. 10.1146/annurev.pharmtox.43.100901.140251.

    Article  CAS  PubMed  Google Scholar 

  26. Kolars JC, Lown KS, Schmiedlin-Ren P, Ghosh M, Fang C, Wrighton SA, Merion RM, Watkins PB: CYP3A gene expression in human gut epithelium. Pharmacogenetics. 1994, 4: 247-259. 10.1097/00008571-199410000-00003.

    Article  CAS  PubMed  Google Scholar 

  27. Windmill KF, McKinnon RA, Zhu X, Gaedigk A, Grant DM, McManus ME: The role of xenobiotic metabolizing enzymes in arylamine toxicity and carcinogenesis: functional and localization studies. Mutat Res. 1997, 376: 153-160.

    Article  CAS  PubMed  Google Scholar 

  28. Bergheim I, Bode C, Parlesak A: Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa. BMC Clin Pharmacol. 2005, 5: 4-10.1186/1472-6904-5-4.

    Article  PubMed  PubMed Central  Google Scholar 

  29. Martinez C, Garcia-Martin E, Pizarro RM, Garcia-Gamito FJ, Agundez JA: Expression of paclitaxel-inactivating CYP3A activity in human colorectal cancer: implications for drug therapy. Br J Cancer. 2002, 87: 681-686. 10.1038/sj.bjc.6600494.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Parke DV: The cytochromes P450 and mechanisms of chemical carcinogenesis. Environ Health Perspect. 1994, 102: 852-853. 10.2307/3432116.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Boocock DJ, Brown K, Gibbs AH, Sanchez E, Turteltaub KW, White IN: Identification of human CYP forms involved in the activation of tamoxifen and irreversible binding to DNA. Carcinogenesis. 2002, 23: 1897-1901. 10.1093/carcin/23.11.1897.

    Article  CAS  PubMed  Google Scholar 

  32. Patten CJ, Smith TJ, Friesen MJ, Tynes RE, Yang CS, Murphy SE: Evidence for cytochrome P450 2A6 and 3A4 as major catalysts for N'-nitrosonornicotine alpha-hydroxylation by human liver microsomes. Carcinogenesis. 1997, 18: 1623-1630. 10.1093/carcin/18.8.1623.

    Article  CAS  PubMed  Google Scholar 

  33. Yamazaki H, Inui Y, Wrighton SA, Guengerich FP, Shimada T: Procarcinogen activation by cytochrome P450 3A4 and 3A5 expressed in Escherichia coli and by human liver microsomes. Carcinogenesis. 1995, 16: 2167-2170. 10.1093/carcin/16.9.2167.

    Article  CAS  PubMed  Google Scholar 

  34. Gallagher EP, Wienkers LC, Stapleton PL, Kunze KL, Eaton DL: Role of human microsomal and human complementary DNA-expressed cytochromes P4501A2 and P4503A4 in the bioactivation of aflatoxin B1. Cancer Res. 1994, 54: 101-108.

    CAS  PubMed  Google Scholar 

  35. Crespi CL, Penman BW, Steimel DT, Gelboin HV, Gonzalez FJ: The development of a human cell line stably expressing human CYP3A4: role in the metabolic activation of aflatoxin B1 and comparison to CYP1A2 and CYP2A3. Carcinogenesis. 1991, 12: 355-359. 10.1093/carcin/12.2.355.

    Article  CAS  PubMed  Google Scholar 

  36. Mani C, Pearce R, Parkinson A, Kupfer D: Involvement of cytochrome P4503A in catalysis of tamoxifen activation and covalent binding to rat and human liver microsomes. Carcinogenesis. 1994, 15: 2715-2720. 10.1093/carcin/15.12.2715.

    Article  CAS  PubMed  Google Scholar 

  37. Rebbeck TR, Jaffe JM, Walker AH, Wein AJ, Malkowicz SB: Modification of clinical presentation of prostate tumors by a novel genetic variant in CYP3A4. J Natl Cancer Inst. 1998, 90: 1225-1229. 10.1093/jnci/90.16.1225.

    Article  CAS  PubMed  Google Scholar 

  38. Zeigler-Johnson C, Friebel T, Walker AH, Wang Y, Spangler E, Panossian S, Patacsil M, Aplenc R, Wein AJ, Malkowicz SB, Rebbeck TR: CYP3A4, CYP3A5, and CYP3A43 genotypes and haplotypes in the etiology and severity of prostate cancer. Cancer Res. 2004, 64: 8461-8467. 10.1158/0008-5472.CAN-04-1651.

    Article  CAS  PubMed  Google Scholar 

  39. Zeigler-Johnson CM, Walker AH, Mancke B, Spangler E, Jalloh M, McBride S, Deitz A, Malkowicz SB, Ofori-Adjei D, Gueye SM, Rebbeck TR: Ethnic differences in the frequency of prostate cancer susceptibility alleles at SRD5A2 and CYP3A4. Hum Hered. 2002, 54: 13-21. 10.1159/000066695.

    Article  CAS  PubMed  Google Scholar 

  40. DeMichele A, Aplenc R, Botbyl J, Colligan T, Wray L, Klein-Cabral M, Foulkes A, Gimotty P, Glick J, Weber B, Stadtmauer E, Rebbeck TR: Drug-metabolizing enzyme polymorphisms predict clinical outcome in a node-positive breast cancer cohort. J Clin Oncol. 2005, 23: 5552-5559. 10.1200/JCO.2005.06.208.

    Article  CAS  PubMed  Google Scholar 

  41. Keshava C, McCanlies EC, Weston A: CYP3A4 polymorphisms--potential risk factors for breast and prostate cancer: a HuGE review. Am J Epidemiol. 2004, 160: 825-841. 10.1093/aje/kwh294.

    Article  PubMed  Google Scholar 

  42. Dally H, Edler L, Jager B, Schmezer P, Spiegelhalder B, Dienemann H, Drings P, Schulz V, Kayser K, Bartsch H, Risch A: The CYP3A4*1B allele increases risk for small cell lung cancer: effect of gender and smoking dose. Pharmacogenetics. 2003, 13: 607-618. 10.1097/00008571-200310000-00004.

    Article  CAS  PubMed  Google Scholar 

  43. Plummer SJ, Conti DV, Paris PL, Curran AP, Casey G, Witte JS: CYP3A4 and CYP3A5 genotypes, haplotypes, and risk of prostate cancer. Cancer Epidemiol Biomarkers Prev. 2003, 12: 928-932.

    CAS  PubMed  Google Scholar 

  44. Dandara C, Ballo R, Parker MI: CYP3A5 genotypes and risk of oesophageal cancer in two South African populations. Cancer Lett. 2005, 225: 275-282. 10.1016/j.canlet.2004.11.004.

    Article  CAS  PubMed  Google Scholar 

  45. Agundez JA, Rodriguez I, Olivera M, Ladero JM, Garcia MA, Ribera JM, Benitez J: CYP2D6, NAT2 and CYP2E1 genetic polymorphisms in nonagenarians. Age Ageing. 1997, 26: 147-151. 10.1093/ageing/26.2.147.

    Article  CAS  PubMed  Google Scholar 

  46. Neitzel H: A routine method for the establishment of permanent growing lymphoblastoid cell lines. Hum Genet. 1986, 73: 320-326. 10.1007/BF00279094.

    Article  CAS  PubMed  Google Scholar 

  47. Cavalli SA, Hirata MH, Hirata RD: Detection of MboII polymorphism at the 5' promoter region of CYP3A4. Clin Chem. 2001, 47: 348-351.

    CAS  PubMed  Google Scholar 

  48. van Schaik RH, van der Heiden IP, van den Anker JN, Lindemans J: CYP3A5 variant allele frequencies in Dutch Caucasians. Clin Chem. 2002, 48: 1668-1671.

    CAS  PubMed  Google Scholar 

  49. Garcia-Martin E, Martinez C, Pizarro RM, Garcia-Gamito FJ, Gullsten H, Raunio H, Agundez JA: CYP3A4 variant alleles in white individuals with low CYP3A4 enzyme activity. Clin Pharmacol Ther. 2002, 71: 196-204. 10.1067/mcp.2002.121371.

    Article  CAS  PubMed  Google Scholar 

  50. Agundez JAG, Lozano L, Ladero JM, Sastre J, Cerdan FJ, Diaz-Rubio M, Benitez J: N-acetyltransferase 2 (NAT2) genotype and colorectal carcinoma: risk variability according to tumour site?. Scand J Gastroenterol. 2000, 35: 1087-1091. 10.1080/003655200451225.

    Article  CAS  PubMed  Google Scholar 

  51. Martinez C, Garcia-Martin E, Ladero JM, Sastre J, Garcia-Gamito F, Diaz-Rubio M, Agundez JA: Association of CYP2C9 genotypes leading to high enzyme activity and colorectal cancer risk. Carcinogenesis. 2001, 22: 1323-1326. 10.1093/carcin/22.8.1323.

    Article  CAS  PubMed  Google Scholar 

  52. Home Page of the Human Cytochrome P450 (CYP) Allele Nomenclature Committee. [http://www.cypalleles.ki.se]

  53. Rodriguez-Antona C, Sayi JG, Gustafsson LL, Bertilsson L, Ingelman-Sundberg M: Phenotype-genotype variability in the human CYP3A locus as assessed by the probe drug quinine and analyses of variant CYP3A4 alleles. Biochem Biophys Res Commun. 2005, 338: 299-305. 10.1016/j.bbrc.2005.09.020.

    Article  CAS  PubMed  Google Scholar 

  54. Floyd MD, Gervasini G, Masica AL, Mayo G, George AL, Bhat K, Kim RB, Wilkinson GR: Genotype-phenotype associations for common CYP3A4 and CYP3A5 variants in the basal and induced metabolism of midazolam in European- and African-American men and women. Pharmacogenetics. 2003, 13: 595-606. 10.1097/00008571-200310000-00003.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Ms. Diana Herrero for technical assistance and Prof. James McCue for assistance in language editing. Financial support: Grants FIS 051056 from Fondo de Investigación Sanitaria, Instituto de Salud Carlos III (Madrid, Spain), SAF-2003-00967 from Ministerio de Ciencia y Tecnología, Madrid, Spain and grant SCSS0414 from Junta de Extremadura, Consejeria de Sanidad y Consumo, Merida (Spain).

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Authors and Affiliations

  1. Department of Pharmacology & Psychiatry, University of Extremadura, Badajoz, Spain

    Guillermo Gervasini, Rosa Pizarro, Carmen Martínez, Monserrat García & José AG Agúndez

  2. Department of Biochemistry & Molecular Biology & Genetics, University of Extremadura, Badajoz, Spain

    Elena García-Martín

  3. Service of Gastroenterology, Hospital Clínico San Carlos, Complutense University, Madrid, Spain

    José M Ladero & Manuel Diaz-Rubio

  4. Service of Oncology Hospital Clínico San Carlos, Complutense University, Madrid, Spain

    Javier Sastre

Authors
  1. Guillermo Gervasini
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  2. Elena García-Martín
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  3. José M Ladero
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  9. José AG Agúndez
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Correspondence to José AG Agúndez.

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Competing interests

The author(s) declare that they have no competing interests.

Authors' contributions

GG, EGM, RP, CM and MG made substantial contributions in data acquisition, molecular genetic analyses, statistical analyses and data interpretation, and helped in manuscript preparation.

JML, JS and MDR were involved in the selection, evaluation and care of patients and helped in manuscript preparation.

JAGA conceived the study, reviewed the literature and drafted the manuscript.

All authors read and approved the manuscript.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gervasini, G., García-Martín, E., Ladero, J.M. et al. Genetic variability in CYP3A4 and CYP3A5in primary liver, gastric and colorectal cancer patients. BMC Cancer 7, 118 (2007). https://doi.org/10.1186/1471-2407-7-118

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  • DOI: https://doi.org/10.1186/1471-2407-7-118

Keywords

BMC Cancer

ISSN: 1471-2407

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