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Targeting and killing of glioblastoma with activated T cells armed with bispecific antibodies
- Ian M Zitron†1,
- Archana Thakur†2,
- Oxana Norkina2,
- Geoffrey R Barger3,
- Lawrence G Lum2, 4, 5 and
- Sandeep Mittal1Email author
© Zitron et al.; licensee BioMed Central Ltd. 2013
Received: 23 July 2012
Accepted: 11 February 2013
Published: 22 February 2013
Since most glioblastomas express both wild-type EGFR and EGFRvIII as well as HER2/neu, they are excellent targets for activated T cells (ATC) armed with bispecific antibodies (BiAbs) that target EGFR and HER2.
ATC were generated from PBMC activated for 14 days with anti-CD3 monoclonal antibody in the presence of interleukin-2 and armed with chemically heteroconjugated anti-CD3×anti-HER2/neu (HER2Bi) and/or anti-CD3×anti-EGFR (EGFRBi). HER2Bi- and/or EGFRBi-armed ATC were examined for in vitro cytotoxicity using MTT and 51Cr-release assays against malignant glioma lines (U87MG, U118MG, and U251MG) and primary glioblastoma lines.
EGFRBi-armed ATC killed up to 85% of U87, U118, and U251 targets at effector:target ratios (E:T) ranging from 1:1 to 25:1. Engagement of tumor by EGFRBi-armed ATC induced Th1 and Th2 cytokine secretion by armed ATC. HER2Bi-armed ATC exhibited comparable cytotoxicity against U118 and U251, but did not kill HER2-negative U87 cells. HER2Bi- or EGFRBi-armed ATC exhibited 50—80% cytotoxicity against four primary glioblastoma lines as well as a temozolomide (TMZ)-resistant variant of U251. Both CD133– and CD133+ subpopulations were killed by armed ATC. Targeting both HER2Bi and EGFRBi simultaneously showed enhanced efficacy than arming with a single BiAb. Armed ATC maintained effectiveness after irradiation and in the presence of TMZ at a therapeutic concentration and were capable of killing multiple targets.
High-grade gliomas are suitable for specific targeting by armed ATC. These data, together with additional animal studies, may provide the preclinical support for the use of armed ATC as a valuable addition to current treatment regimens.
Malignant gliomas, the most lethal brain tumor in adults, account for approximately 13,000 deaths annually in the US . Long-term prognosis for glioblastoma patients remains poor despite surgery and chemoradiotherapy. Major reasons for treatment failure include its highly infiltrative nature and chemoresistance. Given the limitations of aggressive multimodality treatment, targeted cell therapy is an attractive therapeutic alternative.
Despite the paucity of studies, development of cell therapy for glioblastomas has been encouraging. Arming anti-CD3 activated T cells (ATC) with bispecific antibodies (BiAb) that target the T cell receptor on one hand and the tumor-associated antigen on the other can redirect the non-MHC restricted cytotoxicity of ATC to lyse tumors. Arming ex vivo expanded T cells with BiAbs may not only improve clinical responses but also minimize toxicity by avoiding the cytokine storm that can occur by systemic infusion of BiAb alone . Arming ATC with HER2Bi or EGFRBi converts every ATC into a specific cytotoxic T cell [3–7]. Our preclinical studies show that armed ATC can target breast , prostate , ovarian  EGFR+ cancers (head & neck, colorectal, pancreatic, lung , neuroblastomas , and CD20+ NHL . ATC armed with HER2Bi were not only able to lyse cancer cells that have high (3+) expression of HER2 but more importantly target and lyse MCF-7 cells that express low or nil HER2 expression  Moreover, armed ATC can kill multiple times, secrete cytokines/chemokines and multiply after engaging tumor cells in vitro. In vivo anti-tumor activity of armed ATC when co-injected with tumor cells to prevent the tumor development or when injected intratumorally into xenograft model of prostate cancer, armed ATC persist in Beige/SCID mice for 91 days in the spleen and bone marrow without interleukin-2 (IL-2) support [8, 11]. Intravenous infusions of armed ATC inhibit tumor growth in the xenograft models in colon  and ovarian cancer . In our phase I clinical trial involving stage IV breast cancer patients who received activated T cells (ATC) armed with anti-CD3×anti-Her2/neu bispecific antibody (HER2Bi), high levels of circulating tumoricidal cytokines and specific cytotoxicity by PBMC were observed . In an earlier trial, using targeted therapy, lymphokine activated killer (LAK) cells armed with chemically heteroconjugated bispecific antibody (anti-CD3MAb x anti-glioma MAb) in 10 patients showed promising clinical results. In 10 patients, 4 patients had regression of tumor and another 4 patients showed histological eradication of remaining tumor cells post surgery with no recurrence in 10–18 months follow-up . ATC armed with HER2Bi and/or anti-CD3×anti-EGFR (EGFRBi) produced by chemical heteroconjugation of anti-CD3 (OKT3) with trastuzumab or cetuximab, respectively, offers a compelling choice for adjuvant immunotherapy following surgery and chemoradiotherapy.
Although immortalized glioma lines can provide useful biologic insights, cell lines from freshly-resected tumors may more accurately represent the behavior of glioma cells in vivo. In this study, we first established that primary glioma cells can be killed by armed ATC and then addressed further questions of therapeutic relevance: 1) Does dual targeting with BiAbs by mixing individual populations of EGFRBi- and HER2Bi-armed ATC or arming ATC with both BiAbs simultaneously enhance specific cytotoxicity? 2) Can CD133 enriched, CD133− and unfractionated tumor cells be killed differentially by armed ATC? 3) Will armed ATC eliminate a temozolomide (TMZ) resistant subline of U251MG? 4) Do armed ATC continue to kill after being irradiated and in the presence of TMZ? 5) Does binding of armed ATC to glioma cell lines induce the secretion of cytokines?
Generation and expansion of activated T cells
Anti-CD3-activated ATC were expanded in culture from human peripheral blood mononuclear cells (PBMC) . Briefly, ATC were produced by activating PBMC with 20 ng/ml of soluble anti-CD3 (OKT3, Ortho Pharmaceutical, Raritan, NJ) and expanded in IL-2 (aldesleukin, Prometheus Laboratories Inc., San Diego, CA) (100 IU/ml) in RPMI 1640 medium supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, and 1% penicillin-streptomycin for 14 days. After culture, ATC were harvested, washed, counted, and resuspended in RPMI 1640 for immediate use or cryopreserved.
In experiments where ATC were irradiated, cells received a single 2500 cGy dose using a blood bank cell irradiator (Nordion, Ottawa, Canada) to prevent lymphocyte proliferation  and determine whether cytotoxic activity of BiAb-armed ATC is radioresistant.
Bispecific antibodies and arming of ATC
Preparation and characteristics of BiAbs have been described previously [3, 6]. HER2Bi was prepared by chemical heteroconjugation of OKT3 and trastuzumab (Herceptin®, Genentech, South San Francisco, CA). EGFRBi was produced by chemical heteroconjugation of OKT3 and cetuximab (Erbitux®, Bristol-Myer Squibb, NY). Anti-CD3×anti-CD20 BiAb (CD20Bi) was made from heteroconjugation of OKT3 and rituximab (Rituxan®, Genentech, South San Francisco, CA) . ATC were armed with BiAbs at 50 ng/106 cells for 1 hour at 4°C, washed, and resuspended in complete RPMI 1640.
Ex vivoprimary glioblastoma lines
Tumor tissue was washed with PBS+EDTA (2 mM), chopped into fragments ≤1 mm, and enzymatically digested using Accumax (Innovative Cell Technologies, San Diego, CA). Fragments of undigested tissue were removed by low g sedimentation and cell clumps were removed by tissue sieves. Contaminating erythrocytes were removed by centrifugation over Ficoll-Hypaque. Viable single cells were counted using trypan blue exclusion. Culture of the ex vivo adherent differentiated glioma cells was carried out in DMEM-F12 medium (Mediatech, Manassas, VA) supplemented with 10% FCS (Atlanta Biologicals, Atlanta, GA), L-glutamine, and gentamicin (10 μg/ml). Propagation of neurospheres containing cells with stem-like properties was performed in Neurobasal medium (Invitrogen, Carlsbad, CA) containing N-2 and B-27 supplements, human recombinant EGF, and human recombinant basic FGF (each at 20 ng/ml) (PeproTech, Rocky Hill, NJ) .
Long-term glioblastoma lines
Glioma cell lines U87MG, U118MG, and U251MG were also cultured as adherent monolayers in the DMEM-F12-based medium. U87 and U251 cells were grown in 6-well plates in medium supplemented with TMZ over a range of concentrations (10—1000 μM). Medium was changed every 3 days, maintaining the original TMZ concentration. Over 2 weeks, growth of U87MG cells was unaffected, whereas loss of some U251MG cells was recognizable at 10 μM and progressively increased such that a few surviving cells were identified at 333 μM TMZ, but none at 1000 μM. The cells selected in 333 μM TMZ were subsequently propagated in medium containing TMZ (333 μM).
Antibodies, cell separation, and cellular phenotyping
Monoclonal antibodies (cetuximab, trastuzumab, and rituximab) were labeled with the N-hydroxysuccinimide ester of Alexa Fluor 488 (Invitrogen, Carlsbad, CA). These reagents were used for flow cytometry at 1—10 μg/ml. CD133+ cells were isolated using magnetic bead separation (Miltenyi Biotec, Auburn, CA). Frequency of CD133+ cells was determined by flow cytometry using phycoerythrin- (PE) conjugated monoclonal anti-CD133/2 antibodies (Miltenyi Biotec, Auburn, CA).
Viability and cytotoxicity assays
Target cells (4x104 in a volume of 0.1 ml) were plated in 96-well flat bottom microtiter plates (Corning Inc., Corning, NY) and allowed to adhere. Effector cells were added in a volume of 0.1 ml to achieve the effector:target ratio (E:T) indicated and the plates incubated overnight, allowing 16 hours for killing to occur. All groups were performed in triplicate. Non-adherent effector cells were removed and viability of the remaining target cells determined using MTT assay. Metabolism of MTT to the formazan product is a measure of residual viable cells, in contrast to 51Cr release, which is a direct measure of cytotoxicity. We validated the use of the former by confirming a linear relationship between viable cell number and MTT signal and also by performing MTT and 51Cr release in parallel and determining the correlation coefficient of the two data sets.
51Cr Release assay
This was performed in 96-well flat-bottom microtiter plates . Target cells (4x104 cells/well) were plated and allowed to adhere overnight, labeled with 51Cr at 37°C for 4 hours, and then washed in situ to remove unincorporated isotope. Subsequently, effectors were added to achieve a given E:T. 51Cr release was measured after 18 hours and percent cytotoxicity calculated as follows: (experimental cpm – spontaneous cpm) / (maximum cpm – spontaneous cpm) × 100. Triplicate determinations were performed and the means and standard errors of the triplicates calculated.
Bio-Plex assay for the measurement of cytokine secretion
Cytokines were quantitated in culture supernatants using 25-plex human cytokine Luminex Assay (Invitrogen, Carlsbad, CA) in the Bio-Plex System (Bio-Rad Lab., Hercules, CA). The multiplex panel includes interleukin-1β (IL-1β), IL-1 receptor antagonist (IL-1Ra), IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-13, IL-17, tumor necrosis factor (TNF)-α, interferon (IFN)-α, IFN-γ, granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage inhibitory protein (MIP)-1α, MIP-1β, interferon-inducible protein (IP)-10, monokine induced by IFN-γ (MIG), eotaxin, regulated on activation normal T cell expressed and secreted (RANTES), and monocyte chemotactic protein (MCP)-1. The limit of detection for these assays is <10 pg/mL based on detectable signal of >2 fold above background. Cytokine concentration was calculated by the Bio-Plex Manager Software using a standard curve derived from recombinant cytokine standards.
Calculations of means and standard error of the mean (±SEM), non-parametric correlation tests, and 1- and 2-way ANOVA were performed using Prism5 (GraphPad Software, San Diego, CA). All experiments were repeated at least 3 times.
Validation of MTT assay
Optimizing the arming dose of bispecific antibody
In the MTT assay, ATC armed with EGFRBi killed effectively at all 3 doses, essentially eliminating all U118 target cells, while ATC armed with HER2Bi showed 100% cytotoxicity at 5 ng and 50 ng arming dose, however, cytotoxic activity was reduced at 500 ng dose (Figure 2A, upper panel). Similarly, the 51Cr release assay showed higher cytotoxicity at 5 and 50 ng of EGFRBi or HER2Bi/106 ATC with decreasing cytotoxicity as the arming dose was increased to 500 ng of HER2Bi or EGFRBi/106 ATC, which could be due to the receptor saturation induced desensitization of ATC. Similar results were observed with irradiated ATC or aATC at 5, 50 and 500 ng arming doses (Figure 2A, lower panel) and based on these results, we armed ATC at 50 ng of HER2Bi or EGFRBi/106 ATC for subsequent experiments. The data with the three donors in all three cell lines (U87MG, U118MG and U251MG) using unarmed ATC or armed ATC at 50 ng dose are shown in Figure 2B. All experiments were repeated at least three times.
Specific killing of long-term glioma cell lines by armed ATC
Flow cytometry analysis of U87MG, U118MG, and U251MG confirmed that all 3 lines show high surface expression of EGFR (80-100% cell positivity) while only the latter two expressed low levels of surface HER2/neu (U118MG: 17.6% and U251MG: 32.5% positive cells). Figure 2C (lower panel) shows the histograms of EGFR and HER2 expression (blue) compared to isotype control (red), and plots for percent positive cells and mean fluorescence intensity (MFI). The three lines were used as targets for ATC armed with EGFRBi, HER2Bi, and CD20Bi. Figure 2C (upper panel) shows residual viability at E:T = 5:1. Unarmed ATC showed no reduction of tumor cell viability; CD20Bi-armed ATC (irrelevant control) also showed no reduction of tumor cell viability for U118 and U251 cells but showed reduced viability (80%) for U87. CD20Bi-armed ATC mediated reduction in the viability against U87 cells could be due to the nonspecific binding of armed ATC to target cells resulting in effector target interaction induced cytotoxicity. In contrast, all 3 tumor cell lines showed significant losses of viability when exposed to EGFRBi-armed ATC. U118MG and U251MG viabilities were reduced by HER2Bi-armed ATC (middle and right columns), consistent with the surface expression data, whereas HER2Bi-armed ATC (left column) failed to reduce the viability of U87MG cells below 100%. The fetal calf serum used to supplement medium was heat-inactivated and preparation of BiAbs effectively eliminates the complement-fixing properties of the Fc regions, suggesting that aATC mediated cytotoxicity cannot be accounted for complement dependent cytotoxicity.
Killing of fresh ex vivoglioma cells
Does the simultaneous Use of Two BiAbs to Arm ATC improve killing glioma targets?
Are CD133 enriched cells, CD133− cells and unfractionated tumor cells killed differentially by armed ATC?
Does chemoresistance confer protection against specific cytotoxicity by armed ATC? Are armed ATC effective after having undergone irradiation?
In the MTT assay, unarmed ATC did not kill glioma targets. The 51Cr release cytotoxicity assay show <20% cytotoxicity for unarmed ATC alone and ATC that receive radiation alone, TMZ alone, and the combination of radiation and TMZ. Radiation alone had no effect on the viability of either HER2Bi- and EGFRBi-armed ATC (MTT data) but may have increased cytotoxicity (51Cr release data) although was not statistically significant (p=0.07). Exposure to TMZ alone showed a 25-28% reduction in MTT-viable cells with 38% reduction in 51Cr specific cytotoxicity. These data suggest that TMZ may be potentially toxic to ATC. Finally, combination of radiation and TMZ shows 50-55% reduction in viable cells, but only 9-15% reduction in cytotoxicity. Irradiation of armed ATC results in 34-38% reduction in MTT-viable cells when TMZ is present, but increased specific cytotoxicity of 38-46% (p=0.025). Therefore, the cytotoxic capacity of armed ATC is not only radioresistant but can kill in the presence of TMZ.
Do glioma cells exhibit suppressive or contact-dependent inhibitory effects on ATC?
Are cytokines secreted by armed ATC?
Three Th1 cytokines (IFN-γ, GM-CSF, and TNF-α) and one Th2 cytokine (IL-13) showed increases in concentration when the armed ATC were incubated with targets cells. The unarmed ATC secreted 500—840 pg IFN-γ/106cells/24 hrs when incubated alone or with target cells, however armed ATC incubated alone secreted little or no IFN-γ, whereas incubation EGFRBi armed ATC with U251 cells elicited induced 1400—2600 pg/ml of IFN-γ secretion. The other cytokines in Figure 8 show very low baseline concentrations that are clearly increased when they are co-incubated with target cells.
High-grade gliomas are aggressive tumors and respond poorly to all treatment modalities. Since these tumors are highly infiltrative, surgical resection leaves a residuum of cells responsible for recurrence. Malignant gliomas are almost uniformly lethal, with a median survival of about 15 months. This underscores the need for effective, non-toxic strategies that can eliminate the residual tumor cells and also, perhaps, immunize the endogenous immune system against the tumor.
Our immunotherapy approach originates from a series of studies, in which T cells have been redirected to EpCam on adenocarcinomas ; HER2/neu on prostate , breast , and ovarian cancer ; EGFR on a variety of tumor types ; and CD20 on malignant B cells . These studies were performed in vitro, in small animal models, and have progressed to clinical trials . In a recent study, we have shown the involvement of Granzyme B (GrzB) and IFN-γ signaling pathways in BiAb armed ATC mediated cytotoxicity of target cells .
This study shows that both long-term glioma lines and primary cultures of freshly-resected glioblastoma are efficiently killed by ATC armed with either HER2Bi or EGFRBi, indicating that both antigens are potential targets. We also showed that killing is not enhanced by using both BiAbs simultaneously. It may be that, in future studies, individual gliomas will show differential expression of HER2/neu and EGFR and that it will be prudent to both phenotype and functionally test each tumor as a target, in order to choose the best BiAb.
Cells with the stem-like property of self-renewal and the ability to differentiate into the bulk population of tumor cells have been identified in a number of different solid tumor types, amongst the best characterized of which are breast  and gliomas . CD133 was the initial marker identified as characterizing the glioma cancer stem cell, although there are subsequent reports of CD133– cells with similar behavior . The important additional features of stem cells are that they have been postulated to be both chemo- and radioresistant and to be responsible for the extensive infiltration seen in gliomas . The ability to kill CD133+ and CD133− cells, as shown above, indicates that these stem-like cells may be susceptible to killing in this system.
We showed that TMZ-resistant U251MG cells are also susceptible to targeted killing and that armed ATC still kill in the presence of TMZ. TMZ-resistant U251MG cells were <1% CD133+. This may be due to the length of time this line has been in culture, but their susceptibility does indicate that glioma cells that do not have stem-like properties but acquire chemoresistance are also suitable targets for BiAb-armed ATC. We also demonstrated the radioresistance of armed ATC effector function and an indication that irradiation of ATC may cause an increase in cytotoxicity. One possible interpretation is that there is a radiosensitive population of cells in the ATC that suppresses cytotoxic activity. Whether this involves active suppression or merely reflects death of the radiosensitive cells remains unclear. These results suggest that patients undergoing conventional chemoradiation may be suitable candidates for treatment with armed ATCs.
Some tumor types have been shown to produce soluble factors that inhibit immune effectors  and others to express membrane molecules, such as FasL that actually kill effectors [23–29]. We ruled-out the former by showing that long-term incubation of ATC in culture supernatants from immortalized malignant glioma lines and ex vivo gliomas and non-neoplastic astrocytes do not inhibit killing activity. We tested the latter, using long-term tumor lines and showed that these, at least, do not diminish the cytotoxic activity of armed ATC. That is, co-culture of armed ATC with glioma cells permits repeat killing .
Finally, we also showed that when armed ATC are incubated with targets, there is increased secretion of three Th1 cytokines (IFN-γ, GM-CSF, and TNF-α) and one Th2 cytokine (IL-13). The armed ATC are the presumed source of the Th1 cytokines and their secretion would serve to activate microglia (IFN-γ), act as an adjuvant for immunization of endogenous lymphocytes (GM-CSF) and potentially augment the killing of target tumor cells (TNF-α). The cellular origin of the IL-13 is probably also the ATC, since CD8+ T cells have been shown to secrete this cytokine . However, an IL-13 receptor is expressed on some glioma cells  and IL-13 has been shown to be an autocrine growth factor for both Reed-Sternberg cells in Hodgkin’s disease [32–35] and pancreatic cancer . The potential contribution of the glioma cells to the increased IL-13 is under investigation.
Several studies have shown promising results in glioblastoma using various immunotherapeutic approaches . Lymphokine-activated killer (LAK) cells generated from PBMC by co-culture with IL-2 have been reported to selectively kill glioma cells in vitro and when placed into the resection cavity with minimal systemic or neurological side effects [38, 39]. In the first study in which cells were targeted to WHO grade III/IV gliomas, LAK cells were treated with a conjugate of anti-CD3 cross-linked to the NE-150 monoclonal antibody which recognizes an epitope of NCAM . In the control group which received untreated LAK cells, 9/10 patients experienced recurrence within 1 year and 8/10 died within 4 years. In the experimental group, 2/10 showed no response, 4/10 showed regression and 4/10 had complete response. No recurrences occurred during 10—18 months of follow-up in the 8 patients showing partial or complete response. Subsequently, others have reported the use of different BiAbs both in vitro and early-stage clinical trials [41–46].
In conclusion, high-grade gliomas appear to be suitable targets for specific targeting by armed ATC. Should these results be confirmed in animal studies, comparable to those performed with other tumor targets, this approach may represent an additional adjuvant treatment for this devastating malignancy.
This work was funded by National Cancer Institute (R01 CA 92344 and R01 CA 140314 to LGL); Leukemia and Lymphoma Society (Grant # 6092–09 and #6066-06 to LGL); Susan G. Komen Foundation Translational Award (BCTR0707125 to LGL); Cancer Center Support Grant (P30 CA022453-25 to LGL); and Wayne State University School of Medicine Startup Funds (to SM); and Karmanos Cancer Institute Strategic Research Initiative Grant (to SM).
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