Linearity of MTT and 51
Cr responses of glioma lines. Upper panel: A representative data showing a linear correlation between OD and cell numbers of U251MG glioma cell line. The indicated numbers of U251MG cells were incubated with MTT for 4 hours under standard conditions. The assay was completed by the solubilization of formazan, the A570 and A650 nm measured, and the difference calculated. Lower panel: Shows correlation between counts per minute (CPM) and % specific cytotoxicity using 51Cr release assay.