Figure 7
From: Targeting and killing of glioblastoma with activated T cells armed with bispecific antibodies
Armed ATC show sequential killing and effector function is unaffected by contact with glioma cells. In the first overnight culture (Culture 1) unarmed ATC or HER2Bi-armed ATC were incubated with SKBR3 or U251MG target cells (first kill) (E:T = 10:1). The effector cells were removed and MTT added to the wells to determine residual viability compared to control target cells to which no effectors had been added. Data are means (± SEM) of 9–11 cultures. After overnight incubation, without any additional IL-2, the unarmed or HER2Bi-armed ATC were used in a second culture (Culture 2), in which they were added to fresh targets or irrelevant targets (E:T = 10:1). After overnight incubation (second kill), the effectors were removed and MTT added to the wells to determine residual viability of the targets. Data are shown as mean (± SEM) of 4–7 cultures in each group.