Germline variants profiling of BRCA1 and BRCA2 in Chinese Hakka breast and ovarian cancer patients
BMC Cancer volume 22, Article number: 842 (2022)
To investigate the prevalence and spectrum of BRCA1 and BRCA2 mutations in Chinese Hakka patients with breast and ovarian cancer.
A total of 1,664 breast or ovarian cancer patients were enrolled for genetic testing at our hospital. Germline mutations of the BRCA gene were analysed by next-generation sequencing, including the coding regions and exon intron boundary regions.
The 1,664 patients included 1,415 (85.04%) breast cancer patients and 245 (14.72%) ovarian cancer patients, while four (0.24%) patients had both the breast and ovarian cancers. A total of 151 variants, including 71 BRCA1 variants and 80 BRCA2 variants, were detected in the 234 (14.06%) patients. The 151 variants included 58 pathogenic variants, 8 likely pathogenic variants, and 85 variants of unknown significance (VUS). A total of 56.25% (18/32) and 65.38% (17/26) of pathogenic variants (likely pathogenic variants are not included) were distributed in exon 14 of BRCA1 and exon 11 of BRCA2, respectively. The most common pathogenic variants among this Hakka population are c.2635G > T (p.Glu879*) (n = 7) in the BRCA1 gene and c.5164_5165del (p.Ser1722Tyrfs*4) (n = 7) in the BRCA2 gene among the Hakka population. A hotspot mutation in the Chinese population, the BRCA1 c.5470_5477del variant was not found in this Hakka population. The prevalence and spectrum of variants in the BRCA genes in the Hakka patients are different from that in other ethnic groups.
The most common pathogenic variant in this population is c.2635G > T in the BRCA1 gene, and c.5164_5165delAG in the BRCA2 gene in this population. The prevalence and spectrum of variants in the BRCA1 and BRCA2 genes in the Hakka patients from southern China are different from those in other ethnic groups.
With the development of the economy and society, women are increasingly stressed at work and in their personal lives. Additionally, and the incidence of breast cancer and ovarian cancer is on the increasing . Worldwide, breast cancer has surpassed lung cancer as the most common cancer in women, and it is the leading cause of cancer death in females. Ovarian cancer is another one of the most common cancers in women and one of the leading causes of death in women . China is in the stage of cancer transition. The cancer spectrum is changing from developing countries to developed countries, and the burden of breast and ovarian cancer is gradually increasing . Germline mutations in breast cancer susceptibility gene 1 (BRCA1) and/or breast cancer susceptibility gene 2 (BRCA2) confer an increased risk of breast and ovarian cancers .
BRCA1 is located on chromosome 17, contains 24 exons and encodes a multidomain protein containing 1,863 amino acids . BRCA2 is located on chromosome 13, contains 27 exons, and encodes a multidomain protein containing 3,418 amino acids . The primary role of the BRCA1 and BRCA2 genes is to maintain the integrity of the genome, and they act as tumour suppressor genes . Germline mutations in the BRCA1 and BRCA2 genes predispose persons to breast and ovarian cancer . Mutations in the human BRCA1 and BRCA2 genes may be race-specific in a given region and region-specific in a given ethnic group [7, 8].
Hakka is a Han ethnic group with a unique genetic background and originates from the Hakka ancestors of the Han nationality in Central China. They migrated southward for many times and united with the ancient Yue residents in Guangdong, Fujian and Jiangxi . Meizhou City is located in the northeastern of Guangdong Province and has a large Hakka population. However, limited information about the BRCA1 and BRCA2 mutations in this population is available in databases. This study retrospectively analysed the results of screening for genetic mutations of the BRCA1 and BRCA2 in breast and ovarian cancer patients among this population.
Materials and methods
A total of 1,664 breast and/or ovarian cancer patients treated at Meizhou People’s Hospital between May 2017 and June 2021 were enrolled. Inclusion criteria: (1) male or female patients diagnosed with breast cancer; (2) female patients diagnosed with ovarian cancer; and (3) Hakka people based on questionnaires about ethnicity. There were no exclusion criteria. These patients underwent BRCA1 and BRCA2 gene germline mutation screening tests. This study was approved by the Ethics Committee of Medicine, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences. All participants signed informed consent in accordance with the Declaration of Helsinki.
BRCA1 and BRCA2 gene mutation screening test using next-generation sequencing (NGS)
A peripheral blood sample (2 mL) was collected from each participant and collected in a tube containing EDTA as an anticoagulant. Genomic DNA was extracted by using the QIAamp DNA Blood Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions. DNA concentration and purity were quantified using a Nanodrop 2000™ Spectrophotometer (ThermoFisher Scientific, Waltham, MA). The DNA samples were sequenced after library construction, template preparation and template enrichment according to the standard operating procedures of the Life Technology Company. Next-generation sequencing was performed on the Ion Proton instrument (Life Technologies) and tested by the CapitalBio Corporation (Beijing, China). The data were analysed by the Torrent Suite 4.4.3 and 5.0.4 (Life Technologies). According to the Human Genome Variation Society (HGVS) guidelines, the genetic variations in this study, were named using the following reference sequences: NM_007294.4 (BRCA1) and NM_000059.4 (BRCA2). There are four grades of variants: pathogenic variants, likely pathogenic variants, variants of uncertain significance (VUS), and likely benign variants.
Genetic counselling and medical advice
Counselling before genetic testing needs to clarify the purpose of patient counselling and explain the risks, benefits and limitations of genetic testing to patients. A comprehensive collection of patient family history data was obtained; genetic risk was assessed based on patient specific information. Consultation after genetic testing included interpretation of test results, follow-up preventive measures or treatment strategies, evaluation of patients’ needs and psychological state after learning the results, and timely giving corresponding psychological intervention measures.
Those patients with negative genetic test results were treated as nonmutant patients and regularly followed up. If the BRCA1/2 genetics test result was VUS, it was recommended to conduct a BRCA1/2 genetic test on the immediate relatives of these patients to comprehensively evaluate the possibility of VUS. For patients with pathogenic mutations, it was necessary to explain the risk of carrying mutated genes from other family members and passing them on to future generations. It was recommended to conduct BRCA1/2 genetic testing for the immediate relatives of these patients.
Guidance for patient treatment
Surgical treatment of cancer patients with BRCA1/2 mutations
Total mastectomy and contralateral prophylactic mastectomy are recommended for BRCA1/2 mutation patients. However, breast-sparing surgery can be an option for breast cancer patients with BRCA1/2 mutations. If the lesions of patients with BRCA1/2 mutant breast cancer are suitable for breast-conserving surgery and the patients are willing to undergo breast-conserving surgery, breast-conserving surgery can be carefully selected on the premise that the risk of ipsilateral breast cancer recurrence/new primary cancer and contralateral breast cancer are informed.
Risk-reducing salpingo-oophorectomy (RRSO) was performed according to the patient's age and BRCA1/2 gene mutation in ovarian cancer patients. Before RRSO was administered, patients were informed of the common sequelae of iatrogenic menopause, including vasomotor symptoms, osteoporosis, decreased libido, vaginal atrophy and dryness, and cardiovascular disease, as well as the benefits and risks of appropriate remedies.
During routine diagnosis and treatment, we will inform patients of possible surgical options and their risks according to the results of BRCA1/2 gene mutations. The choice of surgical procedure is up to the patient.
Chemotherapy and targeted therapy in cancer patients with BRCA1/2 mutations
Poly ADP-ribose polymerase inhibitors (PARPi) therapy can be used for the treatment of early breast cancer patients with BRCA1/2 pathogenic mutations, is a providing effective treatment options for early breast cancer patients. After adjuvant chemotherapy, HER-2 negative breast cancer patients with BRCA1/2 pathogenic mutations may be advised to receive 1 year of Olaparib-targeted therapy postoperatively. PARPi can be used as first-line maintenance therapy for ovarian cancer patients with BRCA1/2 mutations. In addition, Carboplatin may be recommended for advanced triple-negative breast cancer patients with BRCA1/2 mutations.
In clinical treatment, the selection of chemotherapy drugs or targeted drugs needs to be considered comprehensively according to the patient's condition. In the case of informed consent, it is up to the patient to decide which treatment option to choose.
SPSS statistical software version 21.0 was used for data analyses. Continuous variable data are represented as the mean ± SD. Descriptive analysis was used to show the proportions of sex, different age groups, and disease types in subjects, and to compare the frequencies of the BRCA1 and BRCA2 variants among different populations.
A total of 1,664 breast or ovarian cancer patients were included in the present study, including 1,661 (99.8%) women and 3 (0.2%) men. There were 76 patients (4.6%) under the age of 35, 749 cases (45.0%) between the ages of 35 and 50, and 839 cases (50.4%) beyond the age of 50. The mean ages of patients in the < 35, 35–50, and > 50 years age groups were 29.88 ± 4.60, 44.13 ± 4.34 and 58.23 ± 5.96 years, respectively. There were 1,415 patients (85.04%) with breast cancer, 245 patients (14.72%) with ovarian cancer, and 4 patients (0.24%) with both breast and ovarian cancers. The mean ages of patients with breast cancer, ovarian cancer, and both breast and ovarian cancers were 50.03 ± 9.17, 53.78 ± 12.15 and 56.00 ± 8.29 years, respectively. There were 882 (53.0%) patients in clinical stage 0-II, and 717 (43.1%) patients in clinical stage III-IV (Table 1). The results showed that these patients were roughly evenly divided between those under 50 years old and those over 50 years old, and the majority of these patients were breast cancer patients.
Frequency and distribution of BRCA1 and BRCA2 variants in the Hakka population
There were 234 patients (234/1,664, 14.06%) with BRCA gene variants (including pathogenic variants, likely pathogenic variants, and VUS). Among these patients, 125 patients (125/234, 53.42%) had BRCA1 gene variant/variants, 101 patients (101/234, 43.16%) had BRCA2 gene variant/variants, and 8 patients (8/234, 3.42%) had both BRCA1 and BRCA2 gene variants. A total of 151 variants of the BRCA gene (71 BRCA1 variants and 80 BRCA2 variants; including 58 pathogenic variants, 8 likely pathogenic variants, 85 variants of unknown significance (VUS)) were detected. Variants were detected in all exons of the BRCA1 gene except exons 2, 4, 6, 15, 16 and 21 (Fig. 1A). Variants were detected in all exons of the BRCA2 gene except exons 1, 5, 6, 7, 13, 18, 21, 22, 24 and 26 (Fig. 1B). There were 102 patients (102/1,664, 6.13%) with pathogenic and likely pathogenic variants of the BRCA gene, including 90 patients (90/1,664, 5.41%) with pathogenic variants, and 12 patients (12/1,664, 0.72%) with likely pathogenic variants.
Recurrent variants in the BRCA1 and BRCA2 genes in the Hakka population
While 118 of the 151 distinct BRCA variants were observed only once in a patient, 33 BRCA variants were detected in multiple patients (at least two or more patients). Variants in BRCA1 exon 14 were detected in 37 breast cancer patients and 16 ovarian cancer patients; this was the most frequently mutated exon of BRCA1. The next most common exon of BRCA1 with variants was exon 17 (27 breast cancer patients and 12 ovarian cancer patients) (Fig. 2A). Variants in exon 11 of BRCA2 were detected in 57 breast cancer patients and 12 ovarian cancer patients; this was the most frequently mutated exon of BRCA2. The next most common exons of BRCA2 with variants were exon 15 (7 breast cancer patients and 1 ovarian cancer patient) and exon 10 (6 breast cancer patients) (Fig. 2B). There were 25 breast cancer patients with pathogenic variants, 6 with likely pathogenic variants, and 65 with VUS in the BRCA1 gene. There were 22 ovarian cancer patients with pathogenic variants, 3 with likely pathogenic variants, and 16 with VUS in the BRCA1 gene. There were 34 breast cancer patients with pathogenic variants, 3 with likely pathogenic variants, and 55 with VUS in the BRCA2 gene. There were 9 ovarian cancer patients with pathogenic variants and 10 with VUS in the BRCA2 gene (Fig. 2C).
The c.536A > T variant (p.Tyr179Phe, VUS) (n = 36) and c.2635G > T variant (p.Glu879*, pathogenic) (n = 7) in the BRCA1 gene and the c.5164_5165del variant (p.Ser1722Tyrfs*4, pathogenic) (n = 7), c.2339C > G variant (p.Ser780*, pathogenic) (n = 4), and c.2806_2809del variant (p.Ala938Profs*21, pathogenic) (n = 4) in the BRCA2 gene were the most common variants in the Hakka population. The most common pathogenic variant in the BRCA1 gene was c.2635G > T (p.Glu879*) (n = 7), and the most common pathogenic variant in the BRCA2 gene was c.5164_5165del (p.Ser1722Tyrfs*4) (n = 7) (Fig. 2D and E). The detailed information for each variant, including mutation site, amino acid change, and number of patients detected for each mutation in the BRCA gene, is provided in Table 2 (BRCA1 pathogenic and likely pathogenic variants), Table 3 (BRCA2 pathogenic and likely pathogenic variants), Supplemental Table 1 (VUS), and Supplemental Table 2 (likely benign variants), respectively.
Genetic distribution of pathogenic BRCA1 and BRCA2 variants
A total of 58 pathogenic variants (32 variants in BRCA1 gene and 26 variants in BRCA2) and 8 likely pathogenic variants were detected in this study. Furthermore, 56.25% (18/32) and 65.38% (17/26) of pathogenic variants were distributed in exon 14 of BRCA1 and exon 11 of BRCA2, respectively (Fig. 3A). In breast cancer patients, there were 61.90% (13/21) and 69.57% (16/23) of pathogenic variants were distributed in exon 14 of BRCA1 and exon 11 of BRCA2, respectively (Fig. 3B). In ovarian cancer patients, there were 52.94% (9/17) and 75.0% (6/8) of pathogenic variants were distributed in exon 14 of BRCA1 and exon 11 of BRCA2, respectively (Fig. 3C).
Among the 151 variants of the BRCA gene, 58 distinct pathogenic variants were detected in 90 patients. Among these, 8 likely pathogenic variants (6 variants in BRCA1 and 2 variants in BRCA2) were identified in 12 patients, 85 VUS (33 variants in BRCA1 and 53 variants in BRCA2) were identified in 146 patients, and 25 likely benign variants (6 variants in BRCA1 and 19 variants in BRCA2) were identified in 237 patients. In breast cancer patients, there were 21 pathogenic variants, 4 likely pathogenic variants, and 30 VUS in BRCA1, and 23 pathogenic variants, 2 likely pathogenic variants, and 46 VUS in BRCA2. In ovarian cancer patients, there were 17 pathogenic variants, 3 likely pathogenic variants, and 7 VUS in BRCA1 and 8 pathogenic variants and 10 VUS in the BRCA2 (Fig. 3D).
Ethnicity comparison of BRCA1 and BRCA2 pathogenic variants
The high frequency of BRCA1 and BRCA2 variants in the Hakka population was analysed and compared with those from other ethnicities. The most common variants in BRCA1 and BRCA2 among the Hakka, Chinese, other Asian, European, Latin American, Caribbean, and African populations are illustrated in Table 4. The BRCA1 c.68_69delAG was the most pathogenic variant in the Indian population , Ashkenazi Jewish population , Ashkenazi Jewish population in Argentina , Peruvian population , South African Indian population  and South African population . BRCA1 c.5266dupC was the most pathogenic variant in the Polish population , Italian population , and Southern Brazilian population . There were different hotspot mutations among other populations. They are as follows: BRCA1 c.5251C > T and c.4997dup in the Vietnamese population ; BRCA1 c.4508C > A, c.4065_4068delTCAA, and BRCA2 c.3109C > T, c.4829_4830delTG in the Pakistani population ; BRCA1 c.390C > A, c.3627dupA, and BRCA2 c.7480C > T, c.1399A > T in the Korean population ; BRCA1 c.5123C > A, c.211A > G, and BRCA2 c.2806_2809delAAAC, c.6024dupG in the Spanish population ; BRCA1 c.5123C > A, and BRCA2 c.6174delT in the Latin American and the Caribbean populations ; and BRCA1 c.211dupA, c.798_799delTT, and BRCA2 c.1310_1313delAAGA in the North African population . In a recent meta-analysis of BRCA1 and BRCA2 gene variations in Chinese individuals, c.5470_5477delATTGGGCA, c.2612C > T, and c.3548A > G in BRCA1, and c.3109C > T, c.2806_2809delAAAC, and c.5164_5165delAG in BRCA2 were the most common variants . The most common pathogenic variants were c.2635G > T, c.3756_3759delGTCT, and c.4065_4068delTCAA in the BRCA1 gene and c.5164_5165del, c.2339C > G, and c.2806_2809delACAA in the BRCA2 gene among the Hakka population, respectively. These results showed that the hotspots of pathogenic variants in the BRCA genes demonstrate showed race-specific and region-specific differences.
The BRCA genes are an important genes that determines the genetic susceptibility to cancer by participating in the regulation of DNA damage and repair, cell growth and apoptosis and by playing an indispensable role in maintaining the genetic stability of cells [26, 27]. Variants in the BRCA genes can lead to breast and ovarian cancer. Screening for BRCA gene mutations can effectively assess and predict the risk for breast and ovarian cancer. Thus, they can indicate the appropriate intervention to reduce the incidence of the disease and guide a precise treatment.
There are relatively few complete data on BRCA gene mutations in the Chinese population. At present, there is a gap in research on BRCA mutations in breast cancer and ovarian cancer patients in the Chinese population. Both of the BRCA1 and BRCA2 gene fragments are relatively long, with many diverse variants dispersed throughout the genes. Mutation types in different populations vary greatly, making it difficult to identify specific hotspot mutations. Studies have found that certain mutations are more common in certain populations, known as the founder effect, and these are called founder mutations. BRCA founder mutations have been identified in some ethnic groups worldwide. For example, BRCA1 c.68_69delAG, BRCA1 c.5266dupC and BRCA2 c.5946delT in Ashkenazi Jews , and BRCA1 c.5266dupC and BRCA1 c.4035delA are common in Polish patients . The most common pathogenic variant in BRCA1 was c.981_982delAT, and in BRCA2 c.3195_3198delTAAT . The c.303 T > G, c.1623dupG, and c.4122_4123delTG variants in BRCA1 are frequently found in the African patients with breast cancer . The c.5266dupC, c.5177_5180delGAAA, and c.5251C > T variants in BRCA1 and the c.2808_2811delACAA and c.1138delA variants in BRCA2 were the most common variants among breast and ovarian cancer patients from Brazil . BRCA1 ex9-12del is the most common variant in Mexican patients , and BRCA1 c.5095C > T is the most common variant in Arab breast and ovarian cancer patients . BRCA1 c.68_69delAG is the most common variant in South Asian patients  and Latina patients residing in southern California . BRCA2 c.3922G > T is a founder mutation in the Puerto Rican population . The BRCA1 c.5266dupC mutation is recorded as the founder mutation in Italian , Northeastern Romanian , and Turkish populations . BRCA1 c.5266dupC and c.181 T > G are founder mutations in the Polish population . BRCA1 c.3319G > T is a founder mutation in the Western Denmark . Slavic BRCA1 and BRCA2 founder mutations include BRCA1 c.5266dupC, BRCA1 c.4034delA, and BRCA1 c.68_69delAG . BRCA1 c.4136_4137delCT and c.1140dupG are founder mutations in the Middle Eastern population . BRCA1 c.798_799delTT is a founder mutation in the North African population .
In 2016, BRCA1/2 germline mutations were screened in 5,931 unselected Chinese women with breast cancer, and this study found that the BRCA1 c.5470_5477del was the most common variant in this population . In 2017, Lang et al. enrolled 2,991 breast cancer patients and 1,043 healthy individuals in their study. They found that the most common BRCA1 mutation was c.5470_5477del, and the most common BRCA2 mutations were c.470_474del and c.3109C > T . Wang et al. also found that BRCA1 c.5470_5477del was highly prevalent in a population of Chinese women population . Studies have shown that BRCA1 c.5470_5477del was a founder mutation in Chinese Han ovarian cancer patients  and Chinese Han breast cancer patients . A meta-analysis conducted by Kim et al. on population samples from mainland China in 2016 found that BRCA1 c.981_982delAT and BRCA2 c.3195_3198delTAAT were highly prevalent in mainland Chinese population . In 2018, Kwong et al. analysed more than 600 samples from breast cancer patients in Hong Kong and more than 80 samples from Chinese patients who were overseas and found that the BRCA1 c.964delG and BRCA2 c.3109C > T mutations are common in the local population of Hong Kong . In a recent meta-analysis of BRCA1 and BRCA2 gene variations in Chinese individuals, c.5470_5477del, c.2612C > T, and c.3548A > G in BRCA1, and c.3109C > T, c.2806_2809delAAAC, and c.5164_5165delAG in BRCA2 were the most common variants in this population . In general, BRCA1 c.5470_5477del is considered to be a hotspot and founder mutation in the Chinese population.
The BRCA1 c.5470_5477del variant is not found in the Hakka population. Among the Hakka population in this population, the most common BRCA1 pathogenic variant is c.2635G > T (p.Glu879*) in this study. This variant is predicted to encode a truncated nonfunctional protein. BRCA1 c.2635G > T, a reported mutation among Hong Kong Chinese patients [50, 51], patients with breast cancer from Malaysia , and breast and/or ovarian cancer patients from Singapore [53, 54]. However, this variant is relatively rare in these populations and is not a common variant. This variant is not seen in other populations. Another common mutation BRCA1 c.3756_3759delGTCT has been detected in some populations, such as Thai , Polish , Belarusian , Italian , French-Canadian , and Czech populations . BRCA1 c.4065_4068del has been detected in some populations [61,62,63]. Another study showed that c.4065_4068del is one of the three most common BRCA1 variants in Chinese ovarian cancer patients . In the BRCA2 gene, c.5164_5165delAG has been detected in the Chinese Han population , Macau population , and Taiwanese populations . BRCA2 c.2339C > G has been detected in Taiwanese , and Japanese  individuals. BRCA2 c.2806_2809del has been detected in Mexican individuals .
In addition, there were 3 male breast cancer patients, accounting for 0.21% (3/1430) of the breast cancer patients in this study. Male breast cancer is a rare malignancy that accounts for less than 1% of all breast cancers  in some populations. It accounts for 0.48% of cases in the South Korean populations , 0.6% in the Australian population , 0.9% in the American population , and 0.55% in the Danish population . Of course, there are some populations with higher rates of breast cancer in men. For example, the male breast rate is 1.1% in Northern India , and it is higher in some populations in Africa (6.2% in North Uganda , 2.6% in Burkina Faso , and 3.2% in 27 African countries ). Epidemiological differences between different groups of people may be related to region, race and living environment. Studies have shown that the major risk factors for the development of male breast cancer include advancing age, hormonal imbalance, radiation exposure, and a family history of breast cancer, but the most relevant risk factor is mutations in the BRCA2 gene [79, 80]. None of the three male breast cancer patients in this study had BRCA mutations. Understanding of the biology, clinical manifestations, genetics and treatment of male breast cancer is evolving, but due to the rarity of the disease, it is not well understood at present. More in-depth research is needed.
In general, the prevalence and spectrum of the BRCA1 and BRCA2 genes in the Hakka patients with breast cancer and ovarian cancer from southern China are different from those in other ethnic groups. This study provides a basis and serves as a reference for clinical counselling and the prevention and treatment strategies of breast cancer and ovarian cancer based on genetic screening. Identifying hotspot variants is an effective way to improve genetic counselling because molecular testing can target the hotspot variants, thereby enabling faster and cheaper testing. Clinical BRCA1 and BRCA2 testing enables the identification of individuals at elevated risk for hereditary breast and ovarian cancer. The results of this study can provide local patients with more information about pretest and post test genetic testing. Such information includes why it is indicated, possible test outcomes, implications of the test results for family, economic wellbeing, psychosocial wellbeing, and cancer surveillance and prevention options. Thus, genetic counselling was provided to patients.
Although this study has identified some hotspot variants in the Hakka population, we cannot rule out the possibility that other hotspot variants may exist in a larger Hakka patient population. This is one of the limitations of this study. In addition, participants were identified as Hakka through questionnaires, and no population genetic information was collected and analysed on these participants in this study. This is another shortcoming of this study. Finally, in clinical treatment, although the mutation of BRCA gene was taken into consideration when choosing treatment options, the correlation between the BRCA gene mutation and the prognosis of different treatment options was not analysed. This is one of the deficiencies of this study. In the future, BRCA gene mutation studies with a larger sample size should be carried out in China, including multiethnic studies, and unified standards should be adopted to establish a more complete BRCA gene mutation database that is consistent with the characteristics of the Chinese population. We believe that this study can complement the BRCA gene mutation information in the Chinese population.
In this study, the BRCA gene mutations accounted for a certain proportion of the patients with breast cancer and ovarian cancer in the Hakka population of southern China. In this population, the most common pathogenic variant in the BRCA1 gene was c.2635G > T, and the most common pathogenic variant in the BRCA2 gene was c.5164_5165delAG in BRCA2 gene in this population. The prevalence and spectrum of variants in the BRCA1 and BRCA2 genes in the Hakka patients from southern China are different from those in other ethnic groups.
Availability of data and materials
The variants generated and/or analysed during the current study are available in the clinVar database (https://www.ncbi.nlm.nih.gov/clinvar/), [the ClinVar accessions for this data are SCV002520768 to SCV002520943].
Samadder NJ, Giridhar KV, Baffy N, Riegert-Johnson D, Couch FJ. Hereditary cancer syndromes-A primer on diagnosis and management: part 1: breast-ovarian cancer syndromes. Mayo Clin Proc. 2019;94(6):1084–98.
Sung H, Ferlay J, Siegel RL. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71(3):209–49.
Feng RM, Zong YN, Cao SM, Xu RH. Current cancer situation in China: good or bad news from the 2018 Global Cancer Statistics? Cancer Commun (Lond). 2019;39(1):22.
Yoshida R. Hereditary breast and ovarian cancer (HBOC): review of its molecular characteristics, screening, treatment, and prognosis. Breast Cancer. 2021;28(6):1167–80.
Hall JM, Lee MK, Newman B, Morrow JE, Anderson LA, Huey B, King MC. Linkage of early-onset familial breast cancer to chromosome 17q21. Science. 1990;250(4988):1684–9.
Algebaly AS, Suliman RS, Al-Qahtani WS. Comprehensive study for BRCA1 and BRCA2 entire coding regions in breast cancer. Clin Transl Oncol. 2021;23(1):74–81.
Bhaskaran SP, Chandratre K, Gupta H, Zhang L, Wang X, Cui J, Kim YC, Sinha S, Jiang L, Lu B, et al. Germline variation in BRCA1/2 is highly ethnic-specific: Evidence from over 30,000 Chinese hereditary breast and ovarian cancer patients. Int J Cancer. 2019;145(4):962–73.
Armstrong N, Ryder S. A systematic review of the international prevalence of BRCA mutation in breast cancer. Clin Epidemiol. 2019;11:543–61.
Wang WZ, Wang CY, Cheng YT, Xu AL, Zhu CL, Wu SF, Kong QP, Zhang YP. Tracing the origins of Hakka and Chaoshanese by mitochondrial DNA analysis. Am J Phys Anthropol. 2010;141(1):124–30.
Singh J, Thota N, Singh S, Padhi S, Mohan P, Deshwal S, Sur S, Ghosh M, Agarwal A, Sarin R, et al. Screening of over 1000 Indian patients with breast and/or ovarian cancer with a multi-gene panel: prevalence of BRCA1/2 and non-BRCA mutations. Breast Cancer Res Treat. 2018;170(1):189–96.
Struewing JP, Hartge P, Wacholder S, Baker SM, Berlin M, McAdams M, Timmerman MM, Brody LC, Tucker MA. The risk of cancer associated with specific mutations of BRCA1 and BRCA2 among Ashkenazi Jews. N Engl J Med. 1997;336(20):1401–8.
Solano AR, Liria NC, Jalil FS, Faggionato DM, Mele PG, Mampel A, Cardoso FC, Podesta EJ. BRCA1 and BRCA2 mutations other than the founder alleles among Ashkenazi Jewish in the population of Argentina. Front Oncol. 2018;8:323.
Abugattas J, Llacuachaqui M, Allende YS, Velásquez AA, Velarde R, Cotrina J, Garcés M, León M, Calderón G, de la Cruz M, et al. Prevalence of BRCA1 and BRCA2 mutations in unselected breast cancer patients from Peru. Clin Genet. 2015;88(4):371–5.
Combrink HM, Oosthuizen J, Visser B, Chabilal N, Buccimazza I, Foulkes WD, van der Merwe NC. Mutations in BRCA-related breast and ovarian cancer in the South African Indian population: a descriptive study. Cancer Genet. 2021;258–259:1–6.
Oosthuizen J, Kotze MJ, Van Der Merwe N, Myburgh EJ, Bester P, van der Merwe NC. Globally Rare BRCA2 Variants With Founder Haplotypes in the South African Population: Implications for Point-of-Care Testing Based on a Single-Institution BRCA1/2 Next-Generation Sequencing Study. Front Oncol. 2020;10:619469.
Kowalik A, Siołek M. BRCA1 founder mutations and beyond in the Polish population: A single-institution BRCA1/2 next-generation sequencing study. PLoS One. 2018;13(7): e0201086.
Foglietta J, Ludovini V. Prevalence and spectrum of BRCA germline variants in central Italian high risk or familial breast/ovarian cancer patients: a monocentric study. Genes (Basel). 2020;11(8):925.
Alemar B, Herzog J, Brinckmann Oliveira Netto C, Artigalás O, Schwartz IVD, MatzenbacherBittar C, Ashton-Prolla P, Weitzel JN. Prevalence of Hispanic BRCA1 and BRCA2 mutations among hereditary breast and ovarian cancer patients from Brazil reveals differences among Latin American populations. Cancer Genet. 2016;209(9):417–22.
Tran VT, Nguyen ST, Pham XD, Phan TH, Nguyen VC, Nguyen HT, Nguyen HP, Doan PTT, Le TA, Nguyen BT, et al. Pathogenic variant profile of hereditary cancer syndromes in a Vietnamese cohort. Front Oncol. 2021;11:789659.
Farooq A, Naveed AK, Azeem Z, Ahmad T. Breast and ovarian cancer risk due to prevalence of BRCA1 and BRCA2 variants in Pakistani population: A Pakistani database report. J Oncol. 2011;2011:632870.
Park JS, Lee ST, Han JW, Kim TI, Nam EJ, Park HS. Difference in risk of breast and ovarian cancer according to putative functional domain regions in Korean BRCA1/2 mutation carriers. Clin Breast Cancer. 2018;18(5):362-373.e361.
de RuizSabando A, UrrutiaLafuente E, García-Amigot F, Alonso Sánchez A, Morales Garofalo L, Moreno S, Ardanaz E, Ramos-Arroyo MA. Genetic and clinical characterization of BRCA-associated hereditary breast and ovarian cancer in Navarra (Spain). BMC Cancer. 2019;19(1):1145.
Dutil J, Golubeva VA, Pacheco-Torres AL, Diaz-Zabala HJ, Matta JL, Monteiro AN. The spectrum of BRCA1 and BRCA2 alleles in Latin America and the Caribbean: a clinical perspective. Breast Cancer Res Treat. 2015;154(3):441–53.
ElBiad O, Laraqui A, El Boukhrissi F, Mounjid C, Lamsisi M, Bajjou T, Elannaz H, Lahlou AI, Kouach J, Benchekroune K, et al. Prevalence of specific and recurrent/founder pathogenic variants in BRCA genes in breast and ovarian cancer in North Africa. BMC Cancer. 2022;22(1):208.
Gao X, Nan X, Liu Y, Liu R, Zang W, Shan G, Gai F, Zhang J, Li L, Cheng G, et al. Comprehensive profiling of BRCA1 and BRCA2 variants in breast and ovarian cancer in Chinese patients. Hum Mutat. 2020;41(3):696–708.
Petsalaki E, Zachos G. DNA damage response proteins regulating mitotic cell division: double agents preserving genome stability. FEBS J. 2020;287(9):1700–21.
Gorodetska I, Kozeretska I, Dubrovska A. BRCA Genes: The Role in Genome Stability, Cancer Stemness and Therapy Resistance. J Cancer. 2019;10(9):2109–27.
Górski B, Jakubowska A, Huzarski T, Byrski T, Gronwald J, Grzybowska E, Mackiewicz A, Stawicka M, Bebenek M, Sorokin D, et al. A high proportion of founder BRCA1 mutations in Polish breast cancer families. Int J Cancer. 2004;110(5):683–6.
Kim YC, Zhao L, Zhang H, Huang Y, Cui J, Xiao F, Downs B, Wang SM. Prevalence and spectrum of BRCA germline variants in mainland Chinese familial breast and ovarian cancer patients. Oncotarget. 2016;7(8):9600–12.
Zhang J, Fackenthal JD, Zheng Y, Huo D, Hou N, Niu Q, Zvosec C, Ogundiran TO, Hennis AJ, Leske MC, et al. Recurrent BRCA1 and BRCA2 mutations in breast cancer patients of African ancestry. Breast Cancer Res Treat. 2012;134(2):889–94.
Gallardo-Rincón D, Álvarez-Gómez RM, Montes-Servín E, Toledo-Leyva A, Montes-Servín E, Michel-Tello D, Alamilla-García G, Bahena-González A, Hernández-Nava E, Fragoso-Ontiveros V, et al. Clinical Evaluation of BRCA1/2 Mutation in Mexican Ovarian Cancer Patients. Transl Oncol. 2020;13(2):212–20.
Alhuqail AJ, Alzahrani A, Almubarak H, Al-Qadheeb S, Alghofaili L, Almoghrabi N, Alhussaini H, Park BH, Colak D, Karakas B. High prevalence of deleterious BRCA1 and BRCA2 germline mutations in arab breast and ovarian cancer patients. Breast Cancer Res Treat. 2018;168(3):695–702.
Kharel S, Shrestha S, Yadav S, Shakya P, Baidya S, Hirachan S. BRCA1/BRCA2 mutation spectrum analysis in South Asia: a systematic review. J Int Med Res. 2022;50(1):3000605211070757.
Ossa CA, Torres D. Founder and recurrent mutations in BRCA1 and BRCA2 genes in Latin American Countries: state of the art and literature review. Oncologist. 2016;21(7):832–9.
Diaz-Zabala HJ, Ortiz AP, Garland L, Jones K, Perez CM, Mora E, Arroyo N, Oleksyk TK, Echenique M, Matta JL. A recurrent BRCA2 mutation explains the majority of hereditary breast and ovarian cancer syndrome cases in Puerto Rico. Cancers (Basel). 2018;10(11):419.
Artioli G, Giannone G, Valabrega G, Maggiorotto F, Genta S, Pignata S, Lorusso D, Cormio G, Scalone S, Nicoletto MO, et al. Characteristics and outcome of BRCA mutated epithelial ovarian cancer patients in Italy: a retrospective multicenter study (MITO 21). Gynecol Oncol. 2021;161(3):755–61.
Negură L, Duşa CP, Balmuş MI, Azoicăi D, Negură AM, Marinca MV, Miron L. BRCA1 5382insC founder mutation has not a significative recurrent presence in Northeastern Romanian cancer patients. Rom J Morphol Embryol. 2015;56(2):379–85.
Gun-Bilgic D, Aydin-Gumus A, Bilgic A, Cam FS. Mutations of BRCA1/2 Genes in the West of Turkey and Genotype-Phenotype Correlations. Clin Lab. 2022;68(1). https://doi.org/10.7754/Clin.Lab.2021.210425
Perkowska M, BroZek I, Wysocka B, Haraldsson K, Sandberg T, Johansson U, Sellberg G, Borg A, Limon J. BRCA1 and BRCA2 mutation analysis in breast-ovarian cancer families from northeastern Poland. Hum Mutat. 2003;21(5):553–4.
Nielsen HR, Nilbert M, Petersen J, Ladelund S, Thomassen M, Pedersen IS, Hansen TV, Skytte AB, Borg Å, Therkildsen C. BRCA1/BRCA2 founder mutations and cancer risks: impact in the western Danish population. Fam Cancer. 2016;15(4):507–12.
Sokolenko AP, Sokolova TN, Ni VI, Preobrazhenskaya EV, Iyevleva AG, Aleksakhina SN, Romanko AA, Bessonov AA, Gorodnova TV, Anisimova EI, et al. Frequency and spectrum of founder and non-founder BRCA1 and BRCA2 mutations in a large series of Russian breast cancer and ovarian cancer patients. Breast Cancer Res Treat. 2020;184(1):229–35.
Siraj AK, Bu R, Iqbal K. Prevalence, spectrum, and founder effect of BRCA1 and BRCA2 mutations in epithelial ovarian cancer from the Middle East. Hum Mutat. 2019;40(6):729–33.
Laraqui A, Uhrhammer N, Rhaffouli HE, Sekhsokh Y, Lahlou-Amine I, Bajjou T, Hilali F, El Baghdadi J, Al Bouzidi A, Bakri Y, et al. BRCA genetic screening in Middle Eastern and North African: mutational spectrum and founder BRCA1 mutation (c.798_799delTT) in North African. Dis Markers. 2015;2015:194293.
Zhang J, Sun J, Chen J, Yao L, Ouyang T, Li J, Wang T, Fan Z, Fan T, Lin B, et al. Comprehensive analysis of BRCA1 and BRCA2 germline mutations in a large cohort of 5931 Chinese women with breast cancer. Breast Cancer Res Treat. 2016;158(3):455–62.
Lang GT, Shi JX, Hu X, Zhang CH, Shan L, Song CG, Zhuang ZG, Cao AY, Ling H, Yu KD, et al. The spectrum of BRCA mutations and characteristics of BRCA-associated breast cancers in China: Screening of 2,991 patients and 1,043 controls by next-generation sequencing. Int J Cancer. 2017;141(1):129–42.
Wang J, Li W. Germline mutation landscape of Chinese patients with familial breast/ovarian cancer in a panel of 22 susceptibility genes. Cancer Med. 2019;8(5):2074–84.
Li J, Han S, Zhang C, Luo Y, Wang L, Wang P, Wang Y, Xia Q, Wang X, Wei B, et al. Identification of BRCA1:c.5470_5477del as a founder mutation in Chinese ovarian cancer patients. Front Oncol. 2021;11:655709.
Meng H, Yao L, Yuan H, Xu Y, Ouyang T, Li J, Wang T, Fan Z, Fan T, Lin B, et al. BRCA1 c.5470_5477del, a founder mutation in Chinese Han breast cancer patients. Int J Cancer. 2020;146(11):3044–52.
Kwong A, Shin VY, Ma ES, Chan CT, Ford JM, Kurian AW, Tai E. Screening for founder and recurrent BRCA mutations in Hong Kong and US Chinese populations. Hong Kong Med J. 2018;24 Suppl 3(3):4–6.
Kwong A, Wong LP, Wong HN, Law FB, Ng EK, Tang YH, Chan WK, Ho LS, Kwan KH, Poon M, et al. A BRCA2 founder mutation and seven novel deleterious BRCA mutations in southern Chinese women with breast and ovarian cancer. Breast Cancer Res Treat. 2009;117(3):683–6.
Kwong A, Ng EK, Wong CL, Law FB, Au T, Wong HN, Kurian AW, West DW, Ford JM, Ma ES. Identification of BRCA1/2 founder mutations in Southern Chinese breast cancer patients using gene sequencing and high resolution DNA melting analysis. PLoS One. 2012;7(9):e43994.
Wen WX, Allen J, Lai KN, Mariapun S, Hasan SN, Ng PS, Lee DS, Lee SY, Yoon SY, Lim J, et al. Inherited mutations in BRCA1 and BRCA2 in an unselected multiethnic cohort of Asian patients with breast cancer and healthy controls from Malaysia. J Med Genet. 2018;55(2):97–103.
Chan GHJ, Ong PY, Low JJH, Kong HL, Ow SGW, Tan DSP, Lim YW, Lim SE, Lee SC. Clinical genetic testing outcome with multi-gene panel in Asian patients with multiple primary cancers. Oncotarget. 2018;9(55):30649–60.
Wong ESY, Shekar S, Met-Domestici M, Chan C, Sze M, Yap YS, Rozen SG, Tan MH, Ang P, Ngeow J, et al. Inherited breast cancer predisposition in Asians: multigene panel testing outcomes from Singapore. NPJ Genom Med. 2016;1:15003.
Lertwilaiwittaya P, Roothumnong E, Nakthong P, Dungort P, Meesamarnpong C, Tansa-Nga W, Pongsuktavorn K, Wiboonthanasarn S, Tititumjariya W, Thongnoppakhun W, et al. Thai patients who fulfilled NCCN criteria for breast/ovarian cancer genetic assessment demonstrated high prevalence of germline mutations in cancer susceptibility genes: implication to Asian population testing. Breast Cancer Res Treat. 2021;188(1):237–48.
Łukomska A, Menkiszak J, Gronwald J. Recurrent mutations in BRCA1, BRCA2, RAD51C, PALB2 and CHEK2 in polish patients with ovarian cancer. Cancers (Basel). 2021;13(4):849.
Savanevich A, Ashuryk O, Cybulski C, Lubiński J, Gronwald J. BRCA1 and BRCA2 mutations in ovarian cancer patients from Belarus: update. Hered Cancer Clin Pract. 2021;19(1):13.
Vietri MT, D’Elia G, Caliendo G, Casamassimi A, Resse M, Passariello L, Cioffi M, Molinari AM. Double mutation of APC and BRCA1 in an Italian family. Cancer Genet. 2020;244:32–5.
Behl S, Hamel N, de Ladurantaye M, Lepage S, Lapointe R, Mes-Masson AM, Foulkes WD. Founder BRCA1/BRCA2/PALB2 pathogenic variants in French-Canadian breast cancer cases and controls. Sci Rep. 2020;10(1):6491.
Riedlova P, Janoutova J, Hermanova B. Frequency of mutations in BRCA genes and other candidate genes in high-risk probands or probands with breast or ovarian cancer in the Czech Republic. Mol Biol Rep. 2020;47(4):2763–9.
Loza P, Irmejs A, Daneberga Z, Miklasevics E, Berga-Svitina E, Subatniece S, Maksimenko J, Trofimovics G, Tauvena E, Ukleikins S, et al. A novel frequent BRCA1 recurrent variant c.5117G > A (p.Gly1206Glu) identified after 20 years of BRCA1/2 research in the Baltic region: cohort study and literature review. Hered Cancer Clin Pract. 2021;19(1):11.
Abu-Helalah M, Azab B, Mubaidin R, Ali D, Jafar H, Alshraideh H, Drou N, Awidi A. BRCA1 and BRCA2 genes mutations among high risk breast cancer patients in Jordan. Sci Rep. 2020;10(1):17573.
Power R, Leavy C, Nolan C, White N, Clarke R, Cadoo KA, Gallagher DJ, Lowery MA. Prevalence of pancreaticobiliary cancers in Irish families with pathogenic BRCA1 and BRCA2 variants. Fam Cancer. 2021;20(2):97–101.
Dong H, Chandratre K, Qin Y, Zhang J, Tian X, Rong C, Wang N, Guo M, Zhao G, Wang SM. Prevalence of BRCA1/BRCA2 pathogenic variation in Chinese Han population. J Med Genet. 2021;58(8):565–9.
Qin Z, Kuok CN, Dong H, Jiang L, Zhang L, Guo M, Leong HK, Wang L, Meng G, Wang SM. Can population BRCA screening be applied in non-Ashkenazi Jewish populations? Experience in Macau population. J Med Genet. 2021;58(9):587–91.
Chao A, Lin YH. BRCA1/2 mutation status in patients with metachronous breast and ovarian malignancies: clues towards the implementation of genetic counseling. J Gynecol Oncol. 2020;31(3):e24.
Chao A, Chang TC, Lapke N, Jung SM, Chi P, Chen CH, Yang LY, Lin CT, Huang HJ, Chou HH, et al. Prevalence and clinical significance of BRCA1/2 germline and somatic mutations in Taiwanese patients with ovarian cancer. Oncotarget. 2016;7(51):85529–41.
Tokunaga H, Iida K, Hozawa A, Ogishima S, Watanabe Y, Shigeta S, Shimada M, Yamaguchi-Kabata Y, Tadaka S. Novel candidates of pathogenic variants of the BRCA1 and BRCA2 genes from a dataset of 3,552 Japanese whole genomes (3.5KJPNv2). PLoS One. 2021;16(1):e0236907.
Millan Catalan O, Campos-Parra AD. A Multi-center study of BRCA1 and BRCA2 germline mutations in Mexican-Mestizo breast cancer families reveals mutations unreported in Latin American population. Cancers (Basel). 2019;11(9):1246.
Abdelwahab Yousef AJ. Male Breast Cancer: Epidemiology and Risk Factors. Semin Oncol. 2017;44(4):267–72.
Lee EG, Jung SY, Lim MC, Lim J, Kang HS, Lee S, Han JH, Jo H, Won YJ, Lee ES. Comparing the characteristics and outcomes of male and female breast cancer patients in Korea: Korea central cancer registry. Cancer Res Treat. 2020;52(3):739–46.
Lomma C, Chan A. Male breast cancer in Australia. Asia Pac J Clin Oncol. 2021;17(2):e57–62.
Konduri S, Singh M, Bobustuc G, Rovin R, Kassam A. Epidemiology of male breast cancer. Breast. 2020;54:8–14.
Lautrup MD, Thorup SS, Jensen V, Bokmand S, Haugaard K, Hoejris I, Jylling AB, Joernsgaard H, Lelkaitis G, Oldenburg MH, et al. Male breast cancer: a nation-wide population-based comparison with female breast cancer. Acta Oncol. 2018;57(5):613–21.
Suhani S, Kazi M, Parshad R, Seenu V, Verma E, Mathur S, Gupta SD, Haresh KP. An audit of over 1000 breast cancer patients from a tertiary care center of Northern India. Breast Dis. 2020;39(2):91–9.
Pecorella I, Okello TR, Okwang MD. Incidence of male breast carcinoma in North Uganda: a survey at Lacor Hospital, Gulu, during 2009–2016. Breast Dis. 2021;40(2):95–100.
Zongo N, Ouédraogo S, Korsaga-Somé N, Somé OR, Go N, Ouangré E, Zida M, Bonkoungou G, Ouédraogo AS, Bambara AH, et al. Male breast cancer: diagnosis stages, treatment and survival in a country with limited resources (Burkina Faso). World J Surg Oncol. 2018;16(1):4.
Ndom P, Um G, Bell EM, Eloundou A, Hossain NM, Huo D. A meta-analysis of male breast cancer in Africa. Breast. 2012;21(3):237–41.
Khan NAJ, Tirona M. An updated review of epidemiology, risk factors, and management of male breast cancer. Med Oncol. 2021;38(4):39.
Fostira F, Saloustros E, Apostolou P, Vagena A, Kalfakakou D, Mauri D, Tryfonopoulos D, Georgoulias V, Yannoukakos D, Fountzilas G, et al. Germline deleterious mutations in genes other than BRCA2 are infrequent in male breast cancer. Breast Cancer Res Treat. 2018;169(1):105–13.
The author would like to thank other colleagues whom were not listed in the authorship of Center for Precision Medicine, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences for their helpful comments on the manuscript.
This study was supported by the Guangdong Provincial Key Laboratory of Precision Medicine and Clinical Translation Research of Hakka Population (Grant No.: 2018B030322003), Science and Technology Program of Meizhou (Grant No.: 2019B0202001), Key Scientific and Technological Project of Meizhou People’s Hospital (Grant No.: MPHKSTP-20190102), the Basic and Applied Basic Research Foundation of Guangdong Province (Grant No.: 2021A1515220106), and the Scientific Research Cultivation Project of Meizhou People's Hospital (Grant No.: PY-C2020031).
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The study was approved by the Ethics Committee of Medicine, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences. All participants signed informed consent in accordance with the Declaration of Helsinki.
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Zhang, Y., Wu, H., Yu, Z. et al. Germline variants profiling of BRCA1 and BRCA2 in Chinese Hakka breast and ovarian cancer patients. BMC Cancer 22, 842 (2022). https://doi.org/10.1186/s12885-022-09943-0