- Research article
- Open Access
Efficacy of irreversible EGFR-TKIs for the uncommon secondary resistant EGFR mutations L747S, D761Y, and T854A
BMC Cancer volume 17, Article number: 281 (2017)
Non-small cell lung cancer (NSCLC) harboring common epidermal growth factor receptor (EGFR) gene mutations (exon 19 deletion or exon 21 L858R) respond to EGFR tyrosine kinase inhibitors (EGFR-TKIs). The secondary T790 M mutation in exon 20 of the EGFR gene is the most common type of acquired resistance mutation. Several reports have also shown that other secondary mutations (L747S, D761Y and T854A), while uncommon, can induce acquired resistance to first-generation EGFR-TKIs. However, little is known about the anticancer activities of second- or third-generation EGFR-TKIs.
Uncommon secondary mutations were introduced into Ba/F3 cells along with the sensitive EGFR L858R mutation (Ba/F3-L858R/L747S, Ba/F3-L858R/D761Y, and Ba/F3-L858R/T854A), and the sensitivities to various EGFR-TKIs were then investigated.
Both the Ba/F3-L858R/L747S and Ba/F3-L858R/D761Y cell lines exhibited weak resistances to first-generation reversible EGFR-TKIs, while the Ba/F3-L858R/T854A cell line exhibited a strong resistance. In contrast, irreversible EGFR-TKIs, especially third-generation EGFR-TKIs, were capable of overcoming these resistances. Western blot analyses demonstrated that gefitinib (first-generation) inhibited the phosphorylation of EGFR to a lesser extent in cells with these secondary mutations than in cells with the sensitive L858R mutation alone. In contrast, afatinib and osimertinib (second- and third-generation) inhibited the phosphorylation of EGFR in cells with these secondary mutations to a similar extent as that seen in cells with the sensitive L858R mutation alone.
Our experimental findings suggest that irreversible EGFR-TKIs, especially third-generation EGFR-TKIs, can be effective against uncommon secondary mutations and that switching to third-generation EGFR-TKIs could be a promising treatment strategy for patients with acquired resistance because of these uncommon secondary mutations.
Lung cancer is the leading cause of cancer-related mortality worldwide [1, 2]. The epidermal growth factor receptor (EGFR) is recognized as an important molecular target in cancer therapy, and somatic activating mutations of the EGFR gene (EGFR mutations) are known as one of the oncogenic driver mutations in non small cell lung cancer (NSCLC). NSCLCs with EGFR mutations are associated with sensitivity to EGFR tyrosine kinase inhibitors (EGFR-TKIs) .
Gefitinib and erlotinib are first-generation (1G) reversible EGFR-TKIs that are highly effective against NSCLC carrying common activating EGFR mutations (exon 19 deletion or exon 21 L858R) [4–8]. Although most patients respond dramatically to such treatments, the majority eventually experience disease progression . Many studies have revealed several resistance mechanisms and candidates, including the secondary EGFR mutation T790 M  and other uncommon mutations (L747S , D761Y , and T854A ), MET amplification , HER2 amplification , PTEN down-regulation , high-level HGF expression , epithelial-mesenchymal transition , and conversion to small cell lung cancer  (for review, see [20, 21]).
Afatinib, a second-generation (2G) irreversible EGFR-TKI, also exhibits a marked efficacy against NSCLC carrying EGFR mutations, similar to the effects of gefitinib and erlotinib [19, 22]. In addition, afatinib can be effective against uncommon EGFR mutations [23, 24] for which 1G–TKIs are less effective [25, 26]. Apparently, not all EGFR mutations are created equal; thus, different EGFR mutations may have different sensitivities to various EGFR-TKIs .
The secondary T790 M mutation in exon 20 of the EGFR gene is the most common type of acquired resistance mutation. Approximately 50% of cases with acquired resistance to EGFR-TKI therapy carry this T790 M mutation in the kinase domain of EGFR as well as an EGFR-activating mutation [28–30]. Several recent studies have demonstrated that third-generation (3G) irreversible EGFR-TKIs, which are mutant-selective inhibitors, can overcome T790 M-mediated resistance [31–34]. These findings suggest that different EGFR mutations have different sensitivities to EGFR-TKIs.
Although uncommon, there have been several reports showing that other secondary mutations (L747S , D761Y , and T854A ) induce resistance to 1G–TKIs. The anticancer activities of 2G- or 3G–TKIs against these uncommon secondary mutations, however, remain unclear. In the present study, the anticancer activities of various EGFR-TKIs (1G, 2G, or 3G) against uncommon secondary EGFR mutations were investigated in vitro using the murine Ba/F3 cell system. The Ba/F3 cell system is a murine pro-B cell line that is dependent on interleukin-3 (IL-3) for its survival and growth and is a well-validated and widely used cell system. The ability of Ba/F3 cells transfected with a mutated version of the gene to proliferate in the absence of IL-3 indicates an oncogenic ability [35, 36].
Cell cultures and reagents
The murine pro-B cell line Ba/F3 (RCB0805) was provided by the RIKEN Bio Resource Center (Tsukuba, Japan). Ba/F3 cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, St. Louis, MO), supplemented with 10% fetal bovine serum (FBS) (GIBCO BRL, Grand Island, NY) and 10 ng/mL of IL-3 (Cell Signaling Technology) in a humidified atmosphere of 5% CO2 at 37 °C. Gefitinib and erlotinib (1G–TKIs), afatinib, dacomitinib, and neratinib (2G–TKIs), and osimertinib and rociletinib (3G–TKIs) were purchased from Selleck Chemicals (Houston, TX). The structures of these agents are summarized in Fig. 1.
Protein crystal structure
The crystal structure of EGFR was drawn using the PyMOL Molecular Graphics System (Version 1.7.4; Schrodinger, LLC) based on crystal structure information from PDB ID 2ITZ (EGFR L858R mutation in complex with gefitinib), as previously described .
To analyze the prevalence of EGFR L747S, D761Y, and T854A mutations, the Cancer Genome Atlas (TCGA) dataset (http://cancergenome.nih.gov/) [37, 38] and the Catalogue of Somatic Mutations in Cancer (COSMIC) database (http://cancer.sanger.ac.uk/cancergenome/projects/cosmic/) were used.
Plasmid construction, viral production and stable transfectants
The methods used in the present study have been previously described . Briefly, pBABE with a full-length wild-type EGFR cDNA fragment was purchased from Addgene (Cambridge, MA). pBABE constructs encoding the EGFR L858R mutation and the EGFR L858R mutation plus each of the resistant mutations (L858R + L747S, L858R + D761Y, L858R + T854A, and L858R + T790 M) were generated using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa, Otsu, Japan). All primer sequences are available upon request. All the mutations were confirmed using direct sequencing experiments. The pBABE constructs were cotransfected with a pVSV-G vector (Clontech, Mountain View, CA) to generate the viral envelope in gpIRES-293 cells using the FuGENE6 transfection reagent (Roche Diagnostics, Basel, Switzerland) to produce viral particles. After 48 h of transfection, the culture medium was collected and the viral particles were concentrated by centrifugation at 15,000 ×g for 3 h at 4 °C. The viral pellet was then resuspended in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) and was added to Ba/F3 cells. Infected Ba/F3cells were then purified using GFP-based fluorescence-activated cell sorting using the BD FACS Aria Cell Sorter Special Order Research Product (BD Biosciences, Franklin Lakes, NJ).
Rabbit antibodies specific for EGFR, phospho-EGFR, and β-actin were obtained from Cell Signaling (Beverly, MA).
Western blot analysis
Western blot analysis was performed as previously described . Briefly, Transfected Baf/3 cells were cultured to subconfluence and were rinsed with phosphate-buffered saline (PBS) and harvested with Lysis A buffer containing 1% Triton X-100, 20 mM Tris-HCl (pH 7.0), 5 mM EDTA, 50 mM sodium chloride, 10 mM sodium pyrophosphate, 50 mM sodium fluoride, 1 mM sodium orthovanadate, and a protease inhibitor mix (Complete™; Roche Diagnostics). The total -cell lysate was subjected to SDS-PAGE and was blotted onto a polyvinylidene difluoride membranes. After blocking with 2.5% nonfat milk and 3% bovine serum albumin in a TBS buffer (pH 8.0) with 0.1% Tween-20, the membrane was probed with the primary antibody. After rinsing twice with TBS buffer, the membrane was incubated in primary and secondary antibodies, followed by visualization using an enhance chemiluminescence detection system and LAS-4000 (GE Healthcare, Buckinghamshire, UK). When the phosphorylation levels of EGFR and apoptosis-related molecules were investigated after inhibitor exposure, the samples were collected 3 and 8 h after stimulation, respectively.
IL-3 independent cell growth assay
The transfected Ba/F3 cell lines were cultured for 72 h without IL-3 and were then analyzed using a 3,4,5-dimethyl-2H-tetrazolium bromide assay (MTT; Sigma-Aldrich, St. Louis, MO). The experiment was performed in triplicate as previously described .
Growth inhibition assay in vitro
The growth-inhibitory effects of EGFR-TKIs were examined using an MTT assay . When Ba/F3 transfectant cell lines were used, the cells were cultured without IL-3. Each experiment was performed in triplicate, as previously described .
Continuous variables were analyzed using the Student t-test, and the results were expressed as the average and standard deviation (SD). The statistical analyses were two-tailed and were performed using Microsoft Excel (Microsoft, Redmond, WA). A P value of less than 0.05 was considered statistically significant.
Crystal structure of EGFR, sites of L747, D761, and T854, and frequencies of these secondary mutations in the database
The crystal structure of EGFR was drawn using the PyMOL Molecular Graphics System based on crystal structure information from PDB ID 2ITZ (EGFR L858R mutation in complex with gefitinib). L747 is located at the start of the loop between the h3 strand and the α-C-helix, D761 is located in the α-C-helix, and T854 is located in the activation loop of EGFR. As shown in Fig. 2a, these residual positions (L747, D761, and T854) are close to the binding sites for ATP or reversible EGFR-TKIs (Fig. 2a).
In the TCGA dataset, a total of 408 NSCLC samples (230 adenocarcinomas and 178 squamous cell carcinomas) that had not been treated with chemotherapy, including EGFR-TKIs, were analyzed, and 30 samples had EGFR mutations in exons 18–21. One sample had the T790 M mutation coupled with L858R, but none of the other samples carried uncommon secondary mutations. In the COSMIC database, very few cases of L747 (32, 0.17%; P, 17; S, 14; V, 1), D761 (17, 0.092%; Y, 10; N, 5; G, 2), or T854 (7, 0.038%; A, 4; I, 1; P, 1; S, 1) substitution mutations were found among 18,315 EGFR mutations in exons 18–21. These findings suggest that the frequencies of L747, D761, and T854 substitution mutations are very low.
All EGFR mutation (L858R/L747S, L858R/D761Y, or L858R/T854A)-derived Ba/F3 cell lines can grow without IL-3
To investigate the various EGFR-TKIs sensitivities of these uncommon secondary mutations, EGFR-overexpressed Ba/F3 cell lines were created and a Ba/F3 assay was performed. The EGFR L858R mutation was used as a sensitive mutation, and secondary mutations were introduced into the construct along with this L858R mutation (L858R/L747S, L858R/D761Y, L858R/T854A, and L858R/T790 M) (Fig. 2a). EGFR-overexpression was confirmed by western blotting in the transfectant Ba/F3 cell lines (Fig. 2b). All the Ba/F3 cell lines harboring these secondary mutations along with the L858R mutation exhibited IL-3-independent growth, similar to the Ba/F3-L858R cell line (Fig. 2c). The growth rates of the Ba/F3 cells transfected with each of the constructs were not significantly different, since the actual OD values at 72 h after seeding 2 × 103 cells into each well were not significantly different (L858R, 2.23 ± 0.18; L858R/L747S, 2.73 ± 0.27; L858R/D761Y, 3.14 ± 0.21; L858R/T854A, 2.88 ± 0.06).
Sensitivities to various EGFR-TKIs of transfectant Ba/F3 cell lines harboring secondary mutations
A growth inhibitory assay was performed using an MTT assay, and the sensitivities of transfectant Ba/F3 cell lines to various EGFR-TKIs were compared. The growth inhibitory curves and the 50% inhibitory concentrations (IC50) are summarized in Fig. 3 and Table 1. The Ba/F3-L858R/L747S and Ba/F3-L858R/D761Y cell lines were slightly resistant to 1G–TKIs (gefitinib and erlotinib), whereas the Ba/F3-L858R/T854A cell line was markedly resistant. In contrast, the degrees of resistance were weakened by 2G–TKIs (afatinib, dacomitinib, and neratinib). Furthermore, 3G–TKIs (osimertinib, and rociletinib) were as effective against these Ba/F3 cell lines as they were against the Ba/F3-L858R cell line (Fig. 3 and Table 1). In the Ba/F3-L858R/T790 M cell line, a similar tendency was observed (Fig. 3 and Table 1).
Comparison of IC50
To compare the sensitivities, the ratios of IC50 relative to that of the Ba/F3-L858R cell line (sensitive mutation) (IC50 ratios) were calculated, and these ratios are summarized in Table. 1. The IC50 ratios of the 1G–TKIs were around 5-fold in the Ba/F3-L858R/L747S and Ba/F3-L858R/D761Y cell lines, indicating that these secondary mutations induced a weak resistance to 1G–TKIs (Table 1). In contrast, the IC50 ratios of the 2G–TKIs were less than 5-fold and those of the 3G–TKIs were around 1-fold in the Ba/F3-L858R/L747S and Ba/F3-L858R/D761Y cell lines, indicating that these mutations were sensitive to irreversible EGFR-TKIs (Table 1). In Ba/F3-L858R/T854A, the IC50 ratios of the 1G–TKIs were around 50-fold, meaning that this secondary mutation induced a strong resistance to 1G–TKIs (Table 1). Similar to the cells with L747S and D761Y mutations, the Ba/F3-L858R/T854A cell line exhibited enhanced sensitivities to 2G- or 3G–TKIs. In particular, the IC50 ratios of the 3G–TKIs in the Ba/F3-L858R/T854A cell line were similar to those in the Ba/F3-L858R cell line (IC50 ratios, around 1-fold) (Table 1). These findings suggest that irreversible EGFR-TKIs, especially 3G–TKIs, can overcome the resistance induced by uncommon secondary mutations. This tendency was also observed for the T790 M mutation (Table 1).
Inhibitory activities of each generation of EGFR-TKIs for the phosphorylation of EGFR in cell lines with uncommon secondary EGFR mutations
To investigate the differences in the EGFR inhibitory activities of EGFR-TKIs against cells carrying uncommon secondary EGFR mutations, western blotting was performed using each generation of EGFR-TKIs. We used gefitinib, afatinib, and osimertinib as 1G-, 2G-, and 3G–TKIs, respectively. When gefitinib was used to inhibit EGFR, the phosphorylation level of EGFR was significantly reduced in the Ba/F3-L858R cell line in a dose-dependent manner, compared with the phosphorylation levels in other Ba/F3 cell lines harboring an uncommon secondary mutation (Fig. 4). In particular, the phosphorylation level of EGFR in the Ba/F3-L858R/T854A cell line was not reduced even by a high concentration of gefitinib (100 nM). In contrast, irreversible EGFR-TKIs (2G, afatinib; 3G, osimertinib) reduced the phosphorylation level of EGFR in these Ba/F3 cell lines harboring an uncommon secondary mutation to a greater extent (Fig. 4). Especially, osimertinib (3G) reduced the phosphorylation level of EGFR in Ba/F3 cell lines harboring an uncommon secondary mutation to an extent similar to that seen in the Ba/F3-L858R cell line. This tendency is consistent with the difference in sensitivities; therefore, these findings suggest that the difference in sensitivities is caused by the difference in the EGFR inhibitory activities of each generation of EGFR-TKIs in the cell lines with uncommon secondary EGFR mutations.
The EGFR T790 M mutation in exon 20 is the most common secondary mutation and accounts for approximately 50%–60% of cases with acquired resistance to 1G–TKIs, while uncommon EGFR secondary mutations account for 1%–2% of resistant cases [20, 28, 29]. Since a large proportion of NSCLCs harbor EGFR mutations, especially among Asian patients, uncommon secondary mutations should not be ignored despite their relative scarcity. In the present study, we found that Ba/F3 cell lines harboring an uncommon secondary mutation (L858R/L747S, L858R/D761Y, or L858R/T854A), which are associated with resistance to 1G–TKIs, were sensitive to irreversible EGFR-TKIs, especially 3G–TKIs, suggesting that treatment with 3G–TKIs might be effective for the treatment of lesions with uncommon secondary EGFR mutations. Although the efficacy of 2G–TKIs against lesions with uncommon secondary mutations has been demonstrated previously [11–13], to the best of our knowledge, the present study is the first to investigate the in vitro sensitivities of cells with these uncommon secondary mutations to various EGFR-TKIs, demonstrating the efficacy of irreversible EGFR-TKIs, especially 3G–TKIs.
The most common EGFR mutations, exon 19 deletion and L858R, have both an increased affinity for reversible EGFR-TKIs and a decreased affinity for ATP, compared with wild-type EGFR [41, 42]. The common secondary EGFR T790 M mutation only modestly affects the binding of reversible EGFR-TKIs. More importantly, however, it restores the affinity for ATP, similar to that of wild-type EGFR . The L747S mutation occurs at the start of the loop between the h3 strand and the α-C-helix, and the D761Y mutation is predicted to occur in the α-C-helix of EGFR [11, 12, 44]. These residues are adjacent to K745 and E762, which form a salt bridge that interacts with a- and h-phosphates when ATP is present , and are also adjacent to reversible EGFR-TKI binding sites. T854 is located at the “bottom” of the ATP-binding site, on the C-lobe . Notably, the side chain of T854 is within contact distance of erlotinib or gefitinib in the active structure [42, 44] and is within contact distance of lapatinib in the inactive structure . Therefore, these secondary mutations are thought to influence the binding affinity to ATP or reversible EGFR-TKIs. In our present study, the T854A mutation led to a strong resistance to 1G–TKIs, whereas the L747S and D761Y mutations led to weak resistances. The inhibitory activities of 1G–TKIs for the phosphorylation of EGFR in cell lines with these mutations, especially L858R/T854A, were weakened compared with those in cells carrying only the sensitive L858R mutation. In contrast, irreversible EGFR-TKIs, especially 3G–TKIs, were effective against the transfectant Ba/F3 cell lines harboring these secondary mutations, and the inhibitory activities of irreversible EGFR-TKIs for the phosphorylation of EGFR in cells with these mutations were similar to that in cells with the sensitive L858R mutation alone. Although the detailed mechanism of resistance to 1G–TKIs in cells carrying these secondary mutations remains unclear, the use of irreversible EGFR-TKIs (especially 3G–TKIs) that can inhibit EGFR independently of ATP competition might be useful for overcoming these resistances, based on our experimental findings. These findings indicate that secondary mutations might influence the binding affinity to ATP or reversible EGFR-TKIs, consistent with speculations based on crystal structures.
The discovery that 4-anilinoquinazolines exhibit EGFR inhibitory activity led to the development of 1G–TKIs (Fig. 1) . 2G–TKIs have been developed from 4-anilinoquinazoline and bear Michael acceptor groups in the form of a reactive acrylamide, which is capable of forming covalent adducts with C797 of the EGFR protein (Fig. 1) . Therefore, 2G–TKIs have both reversible (ATP competitive) and irreversible (covalent binding to C797) inhibitory effects. In the present study, 2G–TKIs were more effective against cells with these secondary mutations than 1G–TKIs, but 2G–TKIs were less effective against cells with these secondary mutations than against those with the sensitive L858R mutation alone. Since the secondary mutations can influence the binding affinity to ATP or reversible EGFR-TKIs, the lower effectiveness of 2G–TKIs against cells with these secondary mutations, compared with those with the sensitive L858R mutation alone, can be explained by their ATP competitive inhibitory effects. In contrast to 2G–TKIs, 3G–TKIs mainly have an irreversible inhibitory effect (covalent binding to C797), explaining the similarity in sensitivity between cells with secondary mutations and those with the sensitive L858R mutation alone.
Our present study had several limitations. First, the structures of the mutated EGFR after exposure to EGFR-TKIs and the binding affinity to ATP or reversible EGFR-TKIs could not be analyzed, and the detailed mechanisms responsible for these differences in sensitivity remain unclear. Our results, however, did reveal that the resistances induced by secondary mutations can be overcome using irreversible EGFR-TKIs, especially 3G–TKIs, indicating that these secondary mutations can influence the binding affinity to ATP or reversible EGFR-TKIs. Second, to confirm our experimental findings, further clinical data regarding these uncommon secondary mutations is required. Although their frequencies were very low in our database analyses, the exact frequencies remain unknown because the COSMIC database includes cancer types other than NSCLC and most of the analyzed samples (both TCGA and COSMIC) had never been treated with EGFR-TKIs. In addition, the samples were typically analyzed using detection assays that cannot detect uncommon mutations. Therefore, the actual frequencies might be higher than those reported here. Along with the introduction of 3G–TKIs into clinical settings, re-biopsies of tissue to test for acquired resistance are likely to be performed more frequently [19, 32, 33]. These uncommon secondary mutations, however, cannot be detected by most of the detection assays that are presently in clinical use. Therefore, more comprehensive analyses, such as next-generation sequencing, should be introduced into clinical settings so that patients who do not have T790 M but should nevertheless be treated with 3G–TKIs are not missed.
Our present study showed that irreversible EGFR-TKIs, especially 3G–TKIs, can overcome the resistance induced by uncommon secondary mutations (L747S, D761Y, and T854A). Switching to 3G–TKIs might be a promising treatment strategy for acquired resistance arising from uncommon secondary mutations. To confirm these findings, both basic research and clinical research are additionally needed.
First-generation reversible EGFR-TKI
Second-generation irreversible EGFR-TKI
Third-generation irreversible EGFR-TKI
Catalogue of Somatic Mutations in Cancer
Dulbecco’s Modified Eagle’s Medium
Epidermal growth factor receptor
EGFR tyrosine kinase inhibitor
Fetal bovine serum
- IC50 :
50% inhibitory concentration
Non-small cell lung cancer
Roswell Park Memorial Institute
The Cancer Genome Atlas
Siegel R, Ma J, Zou Z, Jemal A. Cancer statistics, 2014. CA Cancer J Clin. 2014;64:9–29.
Weinstein IB, Joe AK. Mechanisms of disease: Oncogene addiction-a rationale for molecular targeting in cancer therapy. Nat Clin Pract Oncol. 2006;3:448–57.
Lynch TJ, Bell DW, Sordella R, Gurubhagavatula S, Okimoto RA, Brannigan BW, Harris PL, Haserlat SM, Supko JG, Haluska FG, et al. Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. N Engl J Med. 2004;350:2129–39.
Maemondo M, Inoue A, Kobayashi K, Sugawara S, Oizumi S, Isobe H, Gemma A, Harada M, Yoshizawa H, Kinoshita I, et al. Gefitinib or chemotherapy for non-small-cell lung cancer with mutated EGFR. New Engl J Med. 2010;362:2380–8.
Mitsudomi T, Morita S, Yatabe Y, Negoro S, Okamoto I, Tsurutani J, Seto T, Satouchi M, Tada H, Hirashima T, et al. Gefitinib versus cisplatin plus docetaxel in patients with non-small-cell lung cancer harbouring mutations of the epidermal growth factor receptor (WJTOG3405): an open label, randomised phase 3 trial. Lancet Oncol. 2010;11:121–8.
Mok TS, Wu YL, Thongprasert S, Yang CH, Chu DT, Saijo N, Sunpaweravong P, Han B, Margono B, Ichinose Y, et al. Gefitinib or carboplatin-paclitaxel in pulmonary adenocarcinoma. New Engl J Med. 2009;361:947–57.
Rosell R, Carcereny E, Gervais R, Vergnenegre A, Massuti B, Felip E, Palmero R, Garcia-Gomez R, Pallares C, Sanchez JM, et al. Erlotinib versus standard chemotherapy as first-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer (EURTAC): a multicentre, open-label, randomised phase 3 trial. Lancet Oncol. 2012;13:239–46.
Zhou C, Wu YL, Chen G, Feng J, Liu XQ, Wang C, Zhang S, Wang J, Zhou S, Ren S, et al. Erlotinib versus chemotherapy as first-line treatment for patients with advanced EGFR mutation-positive non-small-cell lung cancer (OPTIMAL, CTONG-0802): a multicentre, open-label, randomised, phase 3 study. Lancet Oncol. 2011;12:735–42.
Jackman D, Pao W, Riely GJ, Engelman JA, Kris MG, Jänne PA, Lynch T, Johnson BE, Miller VA. Clinical definition of acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors in non-small-cell lung cancer. J Clin Oncol. 2010;28:357–60.
Kobayashi S, Boggon TJ, Dayaram T, Jänne PA, Kocher O, Meyerson M, Johnson BE, Eck MJ, Tenen DG, Halmos B. EGFR mutation and resistance of non-small-cell lung cancer to gefitinib. N Engl J Med. 2005;352:786–92.
Costa DB, Schumer ST, Tenen DG, Kobayashi S. Differential responses to erlotinib in epidermal growth factor receptor (EGFR)-mutated lung cancers with acquired resistance to gefitinib carrying the L747S or T790M secondary mutations. J Clin Oncol. 2008;26:1182–4. author reply 1184-1186
Balak MN, Gong Y, Riely GJ, Somwar R, Li AR, Zakowski MF, Chiang A, Yang G, Ouerfelli O, Kris MG, et al. Novel D761Y and common secondary T790M mutations in epidermal growth factor receptor-mutant lung adenocarcinomas with acquired resistance to kinase inhibitors. Clin Cancer Res. 2006;12:6494–501.
Bean J, Riely GJ, Balak M, Marks JL, Ladanyi M, Miller VA, Pao W. Acquired resistance to epidermal growth factor receptor kinase inhibitors associated with a novel T854A mutation in a patient with EGFR-mutant lung adenocarcinoma. Clin Cancer Res. 2008;14:7519–25.
Engelman JA, Zejnullahu K, Mitsudomi T, Song Y, Hyland C, Park JO, Lindeman N, Gale CM, Zhao X, Christensen J, et al. MET amplification leads to gefitinib resistance in lung cancer by activating ERBB3 signaling. Science. 2007;316:1039–43.
Takezawa K, Pirazzoli V, Arcila ME, Nebhan CA, Song X, de Stanchina E, Ohashi K, Janjigian YY, Spitzler PJ, Melnick MA, et al. HER2 amplification: a potential mechanism of acquired resistance to EGFR inhibition in EGFR-mutant lung cancers that lack the second-site EGFRT790M mutation. Cancer Discov. 2012;2:922–33.
Sos ML, Koker M, Weir Ba, Heynck S, Rabinovsky R, Zander T, Seeger JM, Weiss J, Fischer F, Frommolt P, et al. PTEN loss contributes to erlotinib resistance in EGFR-mutant lung cancer by activation of Akt and EGFR. Cancer Res. 2009;69:3256–61.
Yano S, Wang W, Li Q, Matsumoto K, Sakurama H, Nakamura T, Ogino H, Kakiuchi S, Hanibuchi M, Nishioka Y, et al. Hepatocyte growth factor induces gefitinib resistance of lung adenocarcinoma with epidermal growth factor receptor-activating mutations. Cancer Res. 2008;68:9479–87.
Suda K, Tomizawa K, Fujii M, Murakami H, Osada H, Maehara Y, Yatabe Y, Sekido Y, Mitsudomi T. Epithelial to Mesenchymal transition in an epidermal growth factor receptor-mutant lung cancer cell line with acquired resistance to Erlotinib. J Thorac Oncol. 2011;6:1–10.
Sequist LV, Yang JC, Yamamoto N, O’Byrne K, Hirsh V, Mok T, Geater SL, Orlov S, Tsai CM, Boyer M, et al. Phase III study of afatinib or cisplatin plus pemetrexed in patients with metastatic lung adenocarcinoma with EGFR mutations. J Clin Oncol. 2013;31:3327–34.
Camidge DR, Pao W, Sequist LV. Acquired resistance to TKIs in solid tumours: learning from lung cancer. Nat Rev Clin Oncol. 2014;11:473–81.
Chong CR, Janne PA. The quest to overcome resistance to EGFR-targeted therapies in cancer. Nat Med. 2013;19:1389–400.
Wu YL, Zhou C, Hu CP, Feng J, Lu S, Huang Y, Li W, Hou M, Shi JH, Lee KY, et al. Afatinib versus cisplatin plus gemcitabine for first-line treatment of Asian patients with advanced non-small-cell lung cancer harbouring EGFR mutations (LUX-lung 6): an open-label, randomised phase 3 trial. Lancet Oncol. 2014;15:213–22.
Yang JC, Sequist LV, Geater SL, Tsai CM, Mok TS, Schuler M, Yamamoto N, Yu CJ, Ou SH, Zhou C, et al. Clinical activity of afatinib in patients with advanced non-small-cell lung cancer harbouring uncommon EGFR mutations: a combined post-hoc analysis of LUX-lung 2, LUX-lung 3, and LUX-lung 6. Lancet Oncol. 2015;16:830–8.
Kobayashi Y, Togashi Y, Yatabe Y, Mizuuchi H, Jangchul P, Kondo C, Shimoji M, Sato K, Suda K, Tomizawa K, et al. EGFR Exon 18 mutations in lung cancer: molecular predictors of augmented sensitivity to Afatinib or Neratinib as compared with first- or third-generation TKIs. Clin Cancer Res. 2015;21:5305–13.
Watanabe S, Minegishi Y, Yoshizawa H, Maemondo M, Inoue A, Sugawara S, Isobe H, Harada M, Ishii Y, Gemma A, et al. Effectiveness of gefitinib against non-small-cell lung cancer with the uncommon EGFR mutations G719X and L861Q. J Thorac Oncol. 2014;9:189–94.
De Pas T, Toffalorio F, Manzotti M, Fumagalli C, Spitaleri G, Catania C, Delmonte A, Giovannini M, Spaggiari L, de Braud F, et al. Activity of epidermal growth factor receptor-tyrosine kinase inhibitors in patients with non-small cell lung cancer harboring rare epidermal growth factor receptor mutations. J Thorac Oncol. 2011;6:1895–901.
Kobayashi Y, Azuma K, Nagai H, Kim YH, Togashi Y, Sesumi Y, Chiba M, Shimoji M, Sato K, Tomizawa K, et al. Characterization of EGFR T790M, L792F, and C797S mutations as mechanisms of acquired resistance to afatinib in lung cancer. Mol Cancer Ther. 2016;
Suda K, Mizuuchi H, Maehara Y, Mitsudomi T. Acquired resistance mechanisms to tyrosine kinase inhibitors in lung cancer with activating epidermal growth factor receptor mutation--diversity, ductility, and destiny. Cancer Metastasis Rev. 2012;31:807–14.
Nguyen KS, Kobayashi S, Costa DB. Acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors in non-small-cell lung cancers dependent on the epidermal growth factor receptor pathway. Clin Lung Cancer. 2009;10:281–9.
Yu HA, Pao W. Targeted therapies: Afatinib-new therapy option for EGFR-mutant lung cancer. Nat Rev Clin Oncol. 2013;10:551–2.
Walter AO, Sjin RT, Haringsma HJ, Ohashi K, Sun J, Lee K, Dubrovskiy A, Labenski M, Zhu Z, Wang Z, et al. Discovery of a mutant-selective covalent inhibitor of EGFR that overcomes T790M-mediated resistance in NSCLC. Cancer Discov. 2013;3:1404–15.
Sequist LV, Rolfe L, Allen AR. Rociletinib in EGFR-mutated non-small-cell lung cancer. N Engl J Med. 2015;373:578–9.
Janne PA, Yang JC, Kim DW, Planchard D, Ohe Y, Ramalingam SS, Ahn MJ, Kim SW, Su WC, Horn L, et al. AZD9291 in EGFR inhibitor-resistant non-small-cell lung cancer. N Engl J Med. 2015;372:1689–99.
Cross DA, Ashton SE, Ghiorghiu S, Eberlein C, Nebhan CA, Spitzler PJ, Orme JP, Finlay MR, Ward RA, Mellor MJ, et al. AZD9291, an irreversible EGFR TKI, overcomes T790M-mediated resistance to EGFR inhibitors in lung cancer. Cancer Discov. 2014;4:1046–61.
He M, Capelletti M, Nafa K, Yun CH, Arcila ME, Miller VA, Ginsberg MS, Zhao B, Kris MG, Eck MJ, et al. EGFR exon 19 insertions: a new family of sensitizing EGFR mutations in lung adenocarcinoma. Clin Cancer Res. 2012;18:1790–7.
Yasuda H, Kobayashi S, Costa DB. EGFR exon 20 insertion mutations in non-small-cell lung cancer: preclinical data and clinical implications. Lancet Oncol. 2011;2045:1–9.
The Cancer Genome Atras Network. Comprehensive genomic characterization of squamous cell lung cancers. Nature. 2012;489:519–25.
The Cancer Genome Atras Network. Comprehensive molecular profiling of lung adenocarcinoma. Nature. 2014;511:543–50.
Togashi Y, Sakamoto H, Hayashi H, Terashima M, de Velasco MA, Fujita Y, Kodera Y, Sakai K, Tomida S, Kitano M, et al. Homozygous deletion of the activin a receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer. Mol Cancer. 2014;13:126.
Sogabe S, Togashi Y, Kato H, Kogita A, Mizukami T, Sakamoto Y, Banno E, Terashima M, Hayashi H, de Velasco MA, et al. MEK inhibitor for gastric cancer with MEK1 Gene mutations. Mol Cancer Ther. 2014;13:3098–106.
Carey KD, Garton AJ, Romero MS, Kahler J, Thomson S, Ross S, Park F, Haley JD, Gibson N, Sliwkowski MX. Kinetic analysis of epidermal growth factor receptor somatic mutant proteins shows increased sensitivity to the epidermal growth factor receptor tyrosine kinase inhibitor, erlotinib. Cancer Res. 2006;66:8163–71.
Yun CH, Boggon TJ, Li Y, Woo MS, Greulich H, Meyerson M, Eck MJ. Structures of lung cancer-derived EGFR mutants and inhibitor complexes: mechanism of activation and insights into differential inhibitor sensitivity. Cancer Cell. 2007;11:217–27.
Yun CH, Mengwasser KE, Toms AV, Woo MS, Greulich H, Wong KK, Meyerson M, Eck MJ. The T790M mutation in EGFR kinase causes drug resistance by increasing the affinity for ATP. Proc Natl Acad Sci U S A. 2008;105:2070–5.
Stamos J, Sliwkowski MX, Eigenbrot C. Structure of the epidermal growth factor receptor kinase domain alone and in complex with a 4-anilinoquinazoline inhibitor. J Biol Chem. 2002;277:46265–72.
Wood ER, Truesdale AT, McDonald OB, Yuan D, Hassell A, Dickerson SH, Ellis B, Pennisi C, Horne E, Lackey K, et al. A unique structure for epidermal growth factor receptor bound to GW572016 (Lapatinib): relationships among protein conformation, inhibitor off-rate, and receptor activity in tumor cells. Cancer Res. 2004;64:6652–9.
Juchum M, Günther M, Laufer SA. Fighting cancer drug resistance: opportunities and challenges for mutation-specific EGFR inhibitors. Drug Resist Updat. 2015;20:12–28.
We thank Ms. Tomoko Kitayama and Ms. Ayaka Kurumatani for their technical assistance.
This study was supported in part by a Grant-in-Aid for Research Activity start-up (Y. Togashi; 15H06754). None of the funding bodies played a role in data collection, analysis, or interpretation of data, the writing of the manuscript, or the decision to submit the manuscript for publication.
Availability of data and materials
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
MC and YT designed and participated in the experiments. MC, YT and TM drafted the manuscript. EB, YK, YN, HH and MT carried out the experiments with cells. MV, KS, and YF performed the statistical analysis. TM and KN conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
Y. Togashi has received a lecture fee from Boehringer-Ingelheim, T. Mitsudomi has received lecture fees from Astra-Zeneca, Boehringer-Ingelheim, Chugai and Pfizer and research funding from Astra-Zeneca, Boehringer-Ingelheim, Chugai and Pfizer. K. Nishio has received lecture fees from Chugai, Daiichi Sankyo and Sumitomo Bakelite. The other authors do not have any potential conflicts of interest to report.
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Chiba, M., Togashi, Y., Bannno, E. et al. Efficacy of irreversible EGFR-TKIs for the uncommon secondary resistant EGFR mutations L747S, D761Y, and T854A. BMC Cancer 17, 281 (2017). https://doi.org/10.1186/s12885-017-3263-z
- EGFR mutation
- Secondary resistant mutation
- Irreversible EGFR-TKI