Fig. 3

Growth inhibitory curves of the transfectant Ba/F3 cell lines. The growth inhibitory assay was performed using an MTT assay and was used to compare the sensitivities to various EGFR-TKIs (1G, gefitinib and erlotinib; 2G, afatinib, dacomitinib, and neratinib; 3G, osimertinib and rociletinib). The Ba/F3-L858R/L747S (blue) and Ba/F3-L858R/D761Y (green) cell lines were slightly resistant to 1G–TKIs (gefitinib and erlotinib), whereas the Ba/F3-L858R/T854A cell line (orange) was markedly resistant. In contrast, the degrees of resistance were weakened by the application of 2G–TKIs (afatinib, dacomitinib, and neratinib). Furthermore, 3G–TKIs were as effective against these Ba/F3 cell lines as they were against the Ba/F3-L858R cell line (black). In the Ba/F3-L858R/T790 M cell line (red), a similar tendency was observed. Lines, mean of independent triplicate experiments