BRCA1-associated protein 1 deficiency in lung adenocarcinoma predicts poor outcome and increased tumor invasion
© The Author(s). 2016
Received: 7 December 2014
Accepted: 29 July 2016
Published: 23 August 2016
The major pathological type of non-small cell lung cancer is lung adenocarcinoma (LAC), which has a poor prognosis. BRCA1-associated protein-1 (BAP1) is a newly identified tumor suppressor that regulates a number of cellular functions in somatic malignancies. However, the impact of BAP1 expression in LAC has not been investigated.
A total of 112 cases of LAC and 101 cases of non-neoplastic lung diseases were included in this study. The study focused on BAP1 expression in lung tissues and its relationship to patients’ clinical and pathological features. BAP1 expression was detected by immunohistochemistry. A human LAC cell line NCI-H1299 was transfected with lipofectamine p3xFLAG-BAP1. BAP1 gene expression was silenced in another LAC cell line NCI-H1650, in order to test the inhibitory effect of BAP1 on cell migration and invasion, as well as cell cycle regulation.
BAP1 expression showed a negative correlation with tumorigenesis of LAC (p <0.001) and lymph node metastasis (p = 0.010). High expression of BAP1 predicted longer disease free survival (p = 0.040) and overall survival (p = 0.021) of LAC patients. In functional assays, BAP1 was found to inhibit the migration and invasion of LAC cells, and promoted their apoptosis and necrosis.
We identify BAP1 as a LAC precursor as well as a robust prognostic indicator in LAC patients. This study provides in vitro rationale for the further investigation of BAP1 in preclinical studies.
KeywordsBAP1 Lung adenocarcinoma Invasion Tumor suppressor
Lung cancer is the most common cause of cancer-related death with an annual mortality rate of 18 % worldwide . Non-small cell lung cancer (NSCLC) presents with advanced pathologic features and has a poor prognosis. Lung adenocarcinoma (LAC) is currently the predominant histological subtype of NSCLC  with an average 5-year survival rate of 15 % despite using epidermal growth factor receptor (EGFR)-targeted therapies. This poor outcome is due in a large part to the delay in diagnosis in the early stages and a paucity of treatments against lymph node metastasis [3, 4]. Evidence also suggests that genetic susceptibility also contributes to malignancy risk in LAC .
Ubiquitin proteasome system (UPS) is involved in intracellular protein degradation, playing a crucial role in physiological processes including cell proliferation, apoptosis and migration [6, 7]. Deubiquitination enzymes (DUBs) act as hydrolases, catalyzing the reverse reaction of ubiquitination [8, 9]. So far, six subfamilies of DUBs have been found: the ubiquitin C-terminal hydrolases (UCHs), the ubiquitin-specific proteases (USPs/UBPs), the ovarian tumor proteases (OTUs), the Machado-Joseph disease protein domain proteases (MJDs), the Jab1/MPN domain-associated metalloisopeptidase (JAMM) domain proteins and the monocyte chemotactic protein-induced protein (MCPIP) [9, 10].
BRCA1-associated protein-1 (BAP1), is a 729aa protein and UCH family member, which exerts its DUB function via binding to the RING finger domain of BRCA1 thereby inhibiting its self-ubiquitination [11, 12]. Accumulating evidence has implicated BAP1 as a tumor suppressor since frequent loss of heterozygosity, large rearrangements, deletions and missense mutations of the BAP1 gene have been found in breast tumors and lung cancer [13, 14]. Evidence has indicated that both DUB enzyme activity and nuclear localization are necessary conditions for its tumor suppressor activity [15, 16]. In functional experiments, BAP1 has been shown to suppress lung cancer tumorigenesis in athymic nude mice, promote the cell cycle and induce both apoptosis and necrosis. Moreover, BAP1-mediated regulation of cell growth is independent of wild-type BRCA1 . In contrast, there is little research paper exploring the effect of BAP1 on tumor metastasis, particularly in lung cancer . Recent research has suggested that BAP1 may be a prognostic factor in advanced NSCLC although this has not been thoroughly investigated .
In the present study we show that the deficiency in BAP1 expression is a strong predictor of malignant transformation of LAC cells. We also report a positive correlation between BAP1 and patient outcome and provide evidence that BAP1 overexpression promotes migration and invasion of adenocarcinoma.
Patients and tissue samples
A total of 112 cases of LAC and 101 cases of non-neoplastic lung diseases were collected in Fudan University Shanghai Cancer Center (Shanghai, China) from 2007 to 2011. Non-neoplastic lung diseases include a wide range of pathologic disorders from sclerosing hamartoma, pulmonary tuberculosis, pneumonia to bronchiectasis. Among the 112 cases of LAC, 59 were male and 53 were female, with an age range of 33 to 79 years (mean 57.6). Twelve out of 112 LAC cases were diagnosed as well-differentiated adenocarcinoma, 82 as showing moderate differentiation and the remaining 19 cases as poorly differentiated. According to the Union for International Cancer Control’s TNM staging criteria (2009), 58 patients were considered pathologic stage I or II and 54 with stage III, with no patient with stage IV undergoing surgery. The inclusion criteria were as follows: (a) LAC was confirmed by surgery and pathology; (b) use of bronchoscope specimens was excluded; (c) preoperative chemotherapy or radiotherapy was not received; (d) similar postoperative chemotherapy regimens were conducted. The median follow-up period for all patients was 25 months. Written informed consent was obtained from each patient.
BAP1 immunohistochemical (IHC) staining was performed on all 213 surgical specimens from patients who were treated by pneumonectomy, lobectomy or wedge resection of lung. Formalin-fixed and paraffin-embedded tissues for IHC were dewaxed in xylene and dehydrated in ethanol. Sections were cut at 4-μm thickness, and then rinsed in 0.05 mol/L phosphate buffer solution (PBS) and incubated overnight at 4 °C with anti-BAP1 antibodies (1:200, HPA028814, Sigma, St. Louis, USA). We also employed 3 % hydrogen peroxide for the inhibition of endogenous peroxidase activity. Steps were performed using DAB horseradish peroxidase color development Kit (Sangon Biotech, Shanghai, China) according to the manufacturer’s instructions. We mixed slides of non-neoplastic disease and slides of LAC tissues so that in each batch of assay there were slides from both groups acting as positive and negative controls.
Brown nuclear staining of LAC cells or bronchial epithelial cells is considered positive. Evaluation of IHC staining was conducted on anonymized slides by two independent pathologists by light microscopy (Olympus Corp. BX-50). The labels of slides were carefully covered up so that none of the observers was able to make sure about the patient health information. Intensity of positive immunostaining was graded as low expression (<30) and high expression (≥30 %)  using 30 % as a cut-off point. To model on this method, I presuppose 10 as cut-off point and divide all patients into two groups, less than 10 positive and more than 10 % positive. Then, draw a survive curve of each group, calculate the p10 % value between the two groups. Second, I presuppose 20 % as cut-off point, divide all patients into two groups and calculate the p20 % value between them, and so forth. Then I get the value of p10 %, p20 %, p30 %, up to p90 %. Choose the minimum p value from the former nine numbers, so that the percentage is the ideal cut-off point. Therefore we instead use 30 % as cut-off point according to the literature. Images were captured from an Olympus Corp. Oly 760 video camera.
The human LAC cell lines NCI-H1650, NCI-H1299, SPCA-1 and large cell lung cancer cell line NCI-H460 were purchased from American Type Culture Collection. Cells were initially maintained in RPMI-1640 medium (Gibco, U.S.A.), supplemented with 10 fetal bovine serum and 1 % penicillin and streptomycin, incubated at 37 °C in a humidified atmosphere containing 5 % CO2.
Gene silencing, plasmid DNA construction and transfection
We measured the level of BAP1 background expression in the four lung cancer cell lines mentioned above by western blot. H460 was excluded because it originates in large cell undifferentiated carcinoma. The work on gene silencing was performed on H1650, which overexpresses the BAP1 protein, to obtain an artificial LAC cell line that expresses extremely low levels of BAP1. Meanwhile, we transfected the cell line H1299, which expressed the lowest level of background BAP1, with plasmid DNA to generate a cell line with artificially high expression of BAP1.
Sequences of 3 si oligos
Sequence and antisense sequence
CGG CCU UUC UAG ACA AUC ATT
UGA UUG UCU AGA AAG GCC GTT
GAG GCU GAG AUU GCA AAC UTT
AGU UUG CAA UCU CAG CCU CTT
GGC UGA GAU UGC AAA CUA UTT
AUA GUU UGC AAU CUC AGC CTT
Negative control (scramble RNA)
UUC UCC GAA CGU GUC ACG UTT
ACG UGA CAC GUU CGG AGA ATT
Positive control (GAPDH)
GUA UGA CAA CAG CCU CAA GTT
CUU GAG GCU GUU GUC AUA CTT
RNA extraction and RT-qPCR
Total RNA was extracted from cultured LAC cells using TRIzol from Invitrogen. Quantitative analysis of BAP1 mRNA expression was performed by RT-qPCR and repeated three times for each batch. Primes for BAP1 RNA amplification were 5'-ATGAATAAGGGCTGGCTGGAGCTG-3' (forward) and 5'-GGGTATCAGCTGGTGGGCAAAGAA-3' (reverse). Thermal cycling conditions were as follows: one cycle of 95 °C for 5 min; 40 cycles for 95 °C for 20 s, 58 °C for 30 s, and 68 °C for 45 s; and one cycle of 72 °C for 10 min. The specificity of the PCR amplification was validated by a single peak in the melting curve.
Western blot analysis
Total protein samples, lysed from cells using RIPA lysis buffer (Abcam), were separated using 10 % SDS-PAGE gel electrophoresis and then transferred onto a nitrocellulose membrane. The membrane was blocked with 5 % (w/v) Bovine serum albumin (BSA) in TBST (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 0.1 % Tween-20), and incubated with primary antibody at 4 °C overnight followed by horseradish peroxidase-conjugated secondary antibody in 5 % BSA-TBST the next day for 2 h at room temperature. The immunoreactive bands were visualized using enhanced chemiluminescence with ECL luminescence (Thermo Scientific, Hudson, NH, USA).
Primary antibodies used were anti-GAPDH (#5632–1, Epitomics, Burlingame, CA, USA) as a loading control, anti-cyclinB1 (#4135, Cell Signaling, Boston, Massachusetts, USA), anti-P27 (kip1, #PA5–17830, Lab Vision Corporation, Fremont, CA) and anti-PARP-1 (#ab32138, Abcam, Massachusetts, USA).
Cell proliferation assays
The proliferation of H1650 and H1299 cells transfected with siRNA or p3xFLAG-BAP1 was indirectly assayed using the Cell Counting Kit-8 (CCK-8, Dojindo, Japan), which quantifies cell viability. One hundred microliters of transfected cells (approximately 5 × 103) were then plated into each well of a 96-well plate. At 24 h, 48 h, and 72 h, 10 μl of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The optical density (OD) at 450 nm was measured using an automatic microplate reader (Synergy4; BioTek, Winooski, VT, USA).
For the cell cycle assay, cells were seeded in 6-well plates (2.5 × 105 cells per well) and harvested after 24 h of treatment with blank vector or plasmid DNA, followed by 12 h cultivation in serum-free medium. Cell cycle distribution was determined by flow cytometry using FACS Calibur (Becton-Dickinson and Company, Franklin Lakes, NJ, USA) with propidium iodide (PI) and analyzed using CELL Quest software (Becton Dickinson and Company, CA, US.).
Apoptosis was assessed using the Annexin V-FITC apoptosis detection kit I and FITC-conjugated antibody was purchased from the Beyotime Institute of Biotechnology (Shanghai, China). Cells were seeded in 6-well plates, incubated with doxorubicin (1 μl per well) for 16 h, and harvested as previously described. DNA content was measured after 30 min of staining with PI. Finally, the cells were analyzed with a flow cytometer using an Epics system (Coulter Epics XL) equipped with an argon ion laser operated at a wavelength of 488 nm. Surface exposure of phosphatidylserine in apoptotic cells was measured using the Annexin V-FITC/PI apoptosis detection kit I.
Wound healing assay and Transwell assay
For the wound healing assay, H1650 cells and H1299 cells were grown until 70 % confluent in 6-well plates. Then H1650 cells were transfected with siNC or siBAP1, while H1299 cells were treated with blank vector or p3xFLAG-BAP1 overnight. Cells were scraped off using a pipette tip and washed with PBS twice to remove floating cells and debris. Representative images of cells migrating into the wounds were captured at 0 h, 24 h and 48 h in the same wounded region.
For cell invasion assays, Transwell inserts with an 8-μm pore size for 24-well plates were pre-coated with Matrigel (ECM550, Chemicon). Transfected cells were seeded in the upper chamber (8 × 104 cells per well) in RPMI 1640 serum-free medium. Medium with 20 % FBS was added in the bottom chamber. After 24 h (for H1299 cells) or 48 h (for H1650 cells), the cells on the upper surface of the filter were removed by wiping with a cotton swab, and the invasive cells that penetrated through the Matrigel were counted in 20 random grids under an inverted microscope (40×).
The statistical significance of the correlation between expression of BAP1 and several clinicopathological parameters was assessed by the chi-squared (χ 2) test, as was the correlation between BAP1 expression and malignant status. The probability of cumulative overall survival (OS) and disease free survival (DFS) as a function of time was estimated by the Kaplan–Meier method and the log-rank test. Multivariate survival analysis was performed using the Cox regression model. P values less than 0.05 were considered as statistically significant. SPSS (version 18.0, SPSS, Inc., Chicago, IL) was used for statistical evaluation. Histograms were constructed by Graphpad prism 5. The MW value was detected by ImageJ (Ver 1.48). The comparison of the two groups in the Transwell assay and RT-qPCR was verified by a t test.
BAP1 expression in lung adenocarcinoma and clinicopathologic findings
Correlation between BAP1 expression and oncogenesis
Correlation between BAP1 expression and clinicopathologic parameters of lung adenocarcinoma
Diameter of Tumor (cm)
Post-operative relapse or metastasis
Multivariate Cox regression model for lung adenocarcinoma patients’ survival
95 % CI for Exp(B)
BAP1 inhibits migration and invasion abilities of cells in vitro
BAP1 promotes cell death and suppresses cell proliferation in LAC
Discussion and conclusions
Study of BAP1 expression in malignant and benign lung tissues provides novel insights into the role of this protein in LAC. As a newly identified biomarker, BAP1 is highly expressed in non-neoplastic lung tissues and reversely correlated with tumor invasion and node metastasis. Moreover, BAP1 predicts longer survival in patients with LAC. Functionally, BAP1 promotes apoptosis and necrosis, and down-regulates the migration and invasion abilities of LAC cells. Thus we confirm that BAP1 serves as a cancer precursor as well as a powerful predictor of improved outcome and reduced likelihood of recurrence.
In the present study, we observed that BAP1 expression was significantly less frequent in LAC tissue (28.6 %) than in non-neoplastic lung diseases (90.1 %; Fig. 3; Table 2). This suggests that BAP1 might suppress initial lung tumorigenesis through an unknown mechanism, implying that further experiments on the role of BAP1 in cell apoptosis are required. We subsequently observed that BAP1-mediated cell death displays features implicating both apoptosis and necrosis. Thus, BAP1 might suppress tumorigenesis by facilitating tumor cell death by activation of both these pathways.
Our immunohistochemical and clinical investigations demonstrated that BAP1 expression was inversely correlated with pathologic TNM stage, node status, pleural invasion, rate of relapse and metastasis. According to the TNM classification for lung cancer of 2009, T stage depends on a range of factors including the maximum diameter of the tumor, pleural invasion, distant to the carina, atelectasis or obstructive pneumonitis, nerve invasion and separate tumor nodule(s) . However, a small proportion of cases present with the following characteristics: (a) tumor in the main airway, less than 2 cm from the carina (none in the present study); (b) tumor accompanied by pneumonitis (3 cases); (c) separate tumor nodule(s) (5 cases). In these cases, tumor size as well as pleural invasion determine T stage to a large degree. Considering that BAP1 expression is not associated with tumor size (p = 0.849) but inversely correlated with pleural invasion (p = 0.004) (Table 3), it is reasonable to believe that pleural invasion had the greatest contribution. It is widely accepted that metastasis is a major hallmark of malignancy and cancer cell invasion is one of the essential early events in metastasis. BAP1 may inhibit the progression of LAC by attenuating tumor invasiveness, a function that was supported by the findings of the Transwell assay (Fig. 8). However, BAP1 is closely related to lymph node status (Table 3), suggesting that BAP1 also mediates suppression of cell migration in LAC which is in keeping with the findings of the wound-healing assay (Fig. 7).
The secretion of proteases such as matrix metalloproteinases (MMPs), capable of degrading the extracellular matrix (ECM) to create space through which cells can migrate, is also modulated by BAP1 expression. Specifically, overexpression of certain MMPs, including MMP2, is directly involved in the progression, invasion and metastasis of LAC [19–21]. E-Cadherin plays an essential role in the maintenance of the normal structure and adhesion of cells and its deregulation is associated with tumor invasion and metastasis, being reduced or absent in a vast majority of NSCLC cases [22–24]. Other tumor metastasis-associated proteins, Vimentin for example, are also down-regulated following the silencing of BAP1 . According to the above results, we postulate that BAP1 may regulate these proteins and impact on tumorigenesis, inhibiting invasion and local metastasis.
Previous research has shown BAP1 to be an independent prognostic factor in colorectal carcinoma  and renal-cell carcinoma [27, 28]. This study reveals for the first time that BAP1 is associated with post-operation survival time of LAC, predicting longer OS and DFS. Further prospective studies of large cohorts are necessary to confirm the potential value of BAP1 in predicting the outcome of LAC patients before being put it into clinical practice.
BAP1, BRCA1-associated protein-1; BRCA1, breast cancer 1; BSA, bovine serum albumin; CCK-8, Cell Counting Kit-8; DFS, disease free survival; DUB, Deubiquitination enzyme; ECM, extracellular matrix; EGFR, epidermal growth factor receptor; FACS, fluorescence activated cell sorting; IHC, immunohistochemical; JAMM, Jab1/MPN domain-associated metalloisopeptidase; LAC, lung adenocarcinoma; MCPIP, monocyte chemotactic protein-induced protein; MJD, Machado-Joseph disease; MMP, matrix metalloproteinases; NC, negative control; NCI, National Cancer Institute; NSCLC, non-small cell lung cancer; OS, overall survival; OUT, ovarian tumor proteases; PBS, phosphate buffer solution; PI, propidium iodide; RT-PCR, real-time polymerase chain reaction; UCH, C-terminal hydrolase; UPS, Ubiquitin proteasome system; USP, Ubiquitin-specific proteases
We also thank the FACS facility in Shanghai Cancer Center that provided help with flow cytometry analysis.
This work was supported by Shanghai Science and Technology Commission of Shanghai Municipality (No.10DJ1400500, 10DJ1400501), National Natural Science Foundation of China (81201836), Shanghai Pujiang Program (12PJ1401800), National Basic Research Program of China (973 Program, Grant No. 2013CB733700). We gratefully acknowledge their assistance.
Availability of data and materials
The sequence of BAP1 is consulted at NCBI. NCBI Blast is used for sequence alignment. Other data supporting our findings is contained within the manuscript or references.
CS designed the study, performed most of the experiments and wrote the manuscript; YW contributed to the experiments and provided reagents and materials. PW analyzed the data and reviewed parts of the manuscript; XD revised and completed the final manuscript. All authors have read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
This study was approved by the Regional Ethical Review Board of Shanghai Cancer Center. All patients agreed to use their personal health information, medical records and specimens for research.
All authors were verbally informed of the purpose, procedures, potential risks and benefits, costs and other details. All authors were clear about the information mentioned above, had an opportunity to ask questions, and agree to participate in this research study.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2008. CA Cancer J Clin. 2008;58(2):71–96.View ArticlePubMedGoogle Scholar
- Beer DG, Kardia SL, Huang CC, et al. Gene-expression profiles predict survival of patients with lung adenocarcinoma. Nat Med. 2002;8(8):816–24.PubMedGoogle Scholar
- Parkin DM, Bray F, Ferlay J, et al. Global cancer statistics. CA Cancer J Clin. 2002;55(2):74–108.View ArticleGoogle Scholar
- Ding L, Getz G, Wheeler DA, et al. Somatic mutations affect key pathways in lung adenocarcinoma. Nature. 2008;455(7216):1069–75.View ArticlePubMedPubMed CentralGoogle Scholar
- Vogelstein B, Papadopoulos N, Velculescu VE, et al. Cancer genome landscapes. Science. 2013;339(6127):1546–058.View ArticlePubMedPubMed CentralGoogle Scholar
- Hilt W, Wolf DH. The ubiquitin-proteasome system: past, present and future. Cell Mol Life Sci. 2004;61(13):1545.View ArticlePubMedGoogle Scholar
- Bence NF, Sampat RM, Kopito RR. Impairment of the ubiquitin-proteasome system by protein aggregation. Science. 2001;292(5521):1552–5.View ArticlePubMedGoogle Scholar
- Fang Y, Fu D, Shen XZ. The potential role of ubiquitin c-terminal hydrolases in oncogenesis. Biochim Biophys Acta. 2010;1806(1):1–6.PubMedGoogle Scholar
- Fraile JM, Quesada V, Rodriguez D, et al. Deubiquitinases in cancer: new functions and therapeutic options. Oncogene. 2012;31(19):2373–88.View ArticlePubMedGoogle Scholar
- Liang J, Saad Y, Lei T, et al. MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-kappaB signaling. J Exp Med. 2010;207(13):2959–73.View ArticlePubMedPubMed CentralGoogle Scholar
- Jensen DE, Proctor M, Marquis ST, et al. BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene. 1998;16(9):1097–112.View ArticlePubMedGoogle Scholar
- Fan LH, Tang LN, Yue L, et al. BAP1 is a good prognostic factor in advanced non-small cell lung cancer. Clin Invest Med. 2012;35(4):182–9.Google Scholar
- Buchhagen DL, Qiu L, Etkind P. Homozygous deletion, rearrangement and hypermethylation implicate chromosome region 3p14.3-3p21.3 in sporadic breast-cancer development. Int J Cancer. 1994;57(4):473–9.View ArticlePubMedGoogle Scholar
- Lerman MI, Minna JD. The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. The international lung cancer chromosome 3p21.3 tumor suppressor gene consortium. Cancer Res. 2000;60(21):6116–33.PubMedGoogle Scholar
- Masaki N, Mitsuru E, Sandra P, et al. High Incidence of Somatic BAP1 Alterations in Sporadic Malignant Mesothelioma. J Thorac Oncol. 2015;10(4):565–76.View ArticleGoogle Scholar
- Ventii KH, Devi NS, Friedrich KL, et al. BRCA1-associated protein-1 is a tumor suppressor that requires deubiquitinating activity and nuclear localization. Cancer Res. 2008;68(17):6953–62.View ArticlePubMedPubMed CentralGoogle Scholar
- Matatall KA, Agapova OA, Onken MD, et al. BAP1 deficiency causes loss of melanocytic cell identity in uveal melanoma. BMC Cancer. 2013;13(1):371–82.View ArticlePubMedPubMed CentralGoogle Scholar
- Detterbeck FC, Boffa DJ, Tanoue LT. The new lung cancer staging system. Chest. 2009;136(1):260–71.View ArticlePubMedGoogle Scholar
- Shou Y, Hirano T, Gong Y, et al. Influence of angiogenetic factors and matrix metalloproteinases upon tumour progression in non-small-cell lung cancer. Br J Cancer. 2001;85(11):1706–12.View ArticlePubMedPubMed CentralGoogle Scholar
- Zheng H, Takahashi H, Murai Y, et al. Expressions of MMP-2, MMP-9 and VEGF are closely linked to growth, invasion, metastasis and angiogenesis of gastric carcinoma. Anticancer Res. 2006;26(13):3579–83.PubMedGoogle Scholar
- Salo T, Makela M, Kylmaniemi M, et al. Expression of matrix metalloproteinase-2 and −9 during early human wound healing. Lab Invest. 1994;70(2):176–82.PubMedGoogle Scholar
- Takeichi M. Morphogenetic roles of classic cadherins. Curr Opin Cell Biol. 1995;7(5):619–27.View ArticlePubMedGoogle Scholar
- Mareel M, Bracke M, Van Roy F. Invasion promoter versus invasion suppressor molecules: the paradigm of E-cadherin. Mol Biol Rep. 1994;19(1):45–67.View ArticlePubMedGoogle Scholar
- Takerchi M. Cadherins in cancer: implications for invasion and metastasis. Curr Opin Cell Biol. 1993;5(5):806–11.View ArticleGoogle Scholar
- Satelli A, Li S. Vimentin in cancer and its potential as a molecular target for cancer therapy. Cell Mol Life Sci. 2011;68(18):3033–46.View ArticlePubMedPubMed CentralGoogle Scholar
- Tang J, Xi S, Wang G, et al. Prognostic significance of BRCA1-associated protein 1 in colorectal cancer. Med Oncol. 2013;30(2):541.View ArticlePubMedGoogle Scholar
- Pena-Llopis S, Vega-Rubin-de-Celis S, Liao A, et al. BAP1 loss defines a new class of renal cell carcinoma. Nat Genet. 2012;44(7):751–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Kapur P, Pena-Llopis S, Christie A, et al. Effects on survival of BAP1 and PBRM1 mutations in sporadic clear-cell renal-cell carcinoma: a retrospective analysis with independent validation. Lancet Oncol. 2013;14(2):159–67.View ArticlePubMedPubMed CentralGoogle Scholar