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Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines

BMC Cancer volume 8, Article number: 310 (2008) Cite this article

Abstract

Background

Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. The aim of the present work was to study the growth inhibition of tumor cells by butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans.

Methods

The anti-proliferative activity of B. adolescentis isolates was assessed by XTT assays on three human colon cancer cell lines (Caco-2, HT-29, and SW480). The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-α (TNF-α) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line.

Results

The butanol extract of B. adolescentis SPM0212 dose-dependently inhibited the growth of Caco-2, HT-29, and SW480 cells by 70%, 30%, and 40%, respectively, at 200 μg/mL. Additionally, the butanol extract of B. adolescentis SPM0212 induced macrophage activation and significantly increased the production of TNF-α and NO, which regulate immune modulation and are cytotoxic to tumor cells.

Conclusion

The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.

Peer Review reports

Background

Colon cancer is a serious health problem and remains the leading cause of cancer mortality throughout the world [1]. Colon cancer incidence has rapidly increased as dietary patterns have changed to contain high fat, high protein, low carbohydrate, and low fiber [2, 3]. Colon cancer is the second most common cancer in Korea [4]. Despite recent advances in our understanding of the biological processes resulting in the development of cancer, there remains a need for new and effective agents to control this disease.

Microorganisms, such as Mycobacterium bovis, Streptococcus pyogenes, Corynebacterium parvum, and cellular components of these bacteria have been used as biological response modifiers (BRM) and are beneficial adjuvants to cancer chemotherapy, increasing remission rates and disease-free intervals. However, the side effect profile in clinical applications for human cancer therapy is important, as these bacteria are pathogens [5–7].

The health and nutritional benefits of orally administered probiotic lactic acid bacteria, such as Lactobacillus and Bifidobacteria species, which are a gram-positive and nonpathogenic, has begun to garner an increasing amount of attention [8, 9].

Probiotics, such as lactic acid bacteria (LAB), are living microorganisms that affect the host in a beneficial manner by improving nutritional and microbial balance in the intestinal tract. These probiotic effects increase the immune response, reduce colon cancer, decrease serum cholesterol, and produce antimicrobial substances, such as bacteriocins that inhibit undesirable diarrhea- and disease-causing pathogens in the human intestine [10–18]. In addition, the dietary consumption of B. lactis HN019 enhances natural immunity in healthy elderly subjects [19–21]. Also, viable or heat-killed Lactobacillus and Bifidobacterium species, as well as certain of their cell components, are capable of stimulating the production of hydrogen peroxide, nitric oxide (NO), and cytokines, such as interleukin (IL)-6 and tumor necrosis factor (TNF)-α, in macrophage cell lines [22–24].

Further, several researchers have studied the anti-tumor effects exerted by lactic acid bacteria [25–38]. Sekine et al. detected anti-tumor activity in peptidoglycans isolated from the B. infantis strain, ATCC 15697, and Oda et al. reported anti-tumor activity in polysaccharide fractions originating from Lactobacillus cultures [30, 32]. Glycoproteins detected in the supernatants of Lactobacillus cultures also have anti-tumor effects [33]. Many strains, including L. rhamnosus GG, L. acidophilus, L. casei, B. longum, B. infantis, B. adolescentis, and B. breve, suppress experimental colon tumor incidence [27, 32–38], but the mechanisms of this tumor suppression are unclear [18, 28, 39].

Our goals were to evaluate the effects of Bifidobacterium adolescentis isolated from fecal samples of healthy young Koreans on immunostimulation and anti-proliferation of human colon cancer cell lines in vitro.

Materials and methods

Bacterial Culture

Fecal samples of 20 healthy Koreans (20–30 years old) were collected by BBL's anaerobic sample collection and transport system to maintain anaerobic conditions, and were used within 24 hr. Fecal samples were serially diluted 10-fold from 10-1 to 10-8, and 100 μl was spread onto selective BL agar containing 5% sheep blood. After 48 hr of incubation in anaerobic conditions (Bactron Anaerobic Chamber, Sheldon Manufacturing Inc., USA) at 37°C, brown or reddish-brown colonies 2–3 mm in diameter were selected for further identification [40].

A fructose-6-phosphate phosphoketolase (F6PPK) test was performed [41] to ensure that the colonies selected were Bifidobacteria, and we analyzed the carbohydrate utilization pattern (Table 1). To identify the isolated Bifidobacterium spp. at the species level, 16S rRNA sequencing was performed by Bioleaders (Daejeon, Korea).

Table 1 Sugar utilization of Bifidobacterium adolescentis SPM
Full size table

B. adolescentis SPM0212 was cultured at 37°C for 48 hr on general anaerobic medium (GAM, Nissui Pharm. Co. Ltd., Japan) under anaerobic conditions (90% N2, 5% H2, 5% CO2).

Preparation of B. adolescentisSPM0212 Extract

For the preparation of B. adolescentis SPM0212 butanol extract, cultures were centrifuged (Vision, USA) at 13,000 rpm for 10 min, then the supernatant was removed and collected bacterial cell pellets were washed with autoclaved phosphate-buffered saline. These cell pellets were lyophilized, and this powder (0.095 g) was suspended in 50 ml of distilled water. Then, it was extracted with 50 ml of n-hexane or ethyl acetate or n-butanol. The BuOH fraction was visibly turbid. The organic solvent of extract was concentrated and removed using a rotary vacuum evaporation. The water, n-hexane, and EtOAc fraction was omitted because they showed low activity or no suppressive effect compared with BuOH fraction in the preliminary test.

Cell Culture

The three human colon cancer cell lines (Table 2) and the murine macrophage cell line, RAW 264.7, was obtained from the Korean Cell Line Bank (Seoul, Korea) and the American Type Culture Collection (ATCC), respectively. Caco-2, HT-29, and SW480 cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640) medium, including fetal bovine serum (FBS) and 1% (v/v) penicillin (10,000 U/ml)/streptomycin (10,000 U/ml) (P/S). RAW 264.7 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (with 10% FBS, 1% penicillin/streptomycin). All cultures were incubated at 37°C in a humidified atmosphere with 5% CO2. After they were grown to confluence in 75 cm2 tissue culture flasks (NunC, Denmark), cells were detached and transferred to new cell culture dishes in a trypsin-versene mixture (Cambrex Bio Science, USA). Cell number and viability were assessed by the trypan blue dye-exclusion method [42].

Table 2 Characteristics of cell lines used in this study (KCLB, Korean Cell Line Bank)
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Tumor Cell Proliferation by XTT Assay

Cell proliferation was quantified via an XTT assay (sodium 3-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene sulfonic acid hydrate). Cells were seeded on 96-well microplates (NunC, Denmark) at 3 × 103 cells/well and incubated for 72 hr with the test compounds. Control was only cells (no treated). The butanol extract (no cells) was not tested. The samples were then incubated with 50 μl of XTT solution (1 mg/ml) for 6 hrs and measured with an ELISA reader (Molecular Devices, USA) at 490 nm.

Tumor Necrosis Factor-α (TNF-α) Quantification

RAW 264.7 (1 × 105 cells/ml), LPS (Escherichia coli O127:B8 Westphal type, 100 ng/mL), and test samples (12.5, 25, 50, 100, 200 μg/ml) were prepared as treated groups and incubated for 48 hr. Following incubation, TNF-α secretion was assessed with an OPTEIA™ Mouse TNF-α kit (Pharmingen, San Diego, CA, USA) in accordance with manufacturer's protocol. Briefly, the sample and recombinant standards were added to antibody-coated plates and incubated for 2 hr. TNF-α was detected via the addition of horseradish peroxidase-conjugated, streptavidin-labeled antibodies. Color was developed using tetramethylbenzidine (TMB) (BD Biosciences, Pharmingen, USA) for 30 min and the absorbance was recorded at 450 nm.

Nitric Oxide Assay

RAW 264.7 cells (1 × 106 cells/ml), LPS (50 ng/ml), and test samples (12.5, 25, 50, 100, 200 μg/ml) were prepared and incubated overnight. One hundred microliters from the surface of cultures was transferred into a new plate and the equivalent amount of Griess reagent was added (Stock-1: 0.2% naphylendia HCl, Stock-2: 2% sulfanilamide in 5% H3PO4). This plate was then incubated for 10 min at RT and measured by an ELISA reader at 540 nm. Standard calibration curves were prepared using sodium nitrite as a standard.

Effect of B. adolescentisSPM0212 on Macrophage Morphology

RAW 264.7 cells (1 × 103 cells/well) were cultured in sterile glass-slide chambers for 48 hr. The culture medium was removed, and the cells were treated with either LPS (100 ng/ml) or samples of B. adolescentis SPM0212 (12.5, 25, 50, 100, 200 μg/ml) for 48 hr. Following treatment, the culture supernatant was removed, and the cells were fixed and stained in Diff Quick Solution (Baxter, Houston, TX). Macrophage morphology was observed using a light microscope (BX41, Olympus, Japan) at 400× magnification.

Statistical Analysis

All data were expressed as the mean ± standard deviation (SD). For statistical evaluation of data, one-way ANOVA was applied using the program SPSS 13.0 for Windows. This was followed by post hoc comparisons using the Tukey's test. Significant differences were considered significant at P < 0.05.

Results

B. adolescentisStrains Inhibit the Growth of Colon Cancer Cell Lines

To determine whether B. adolescentis strains inhibit the growth of the colon cancer cell lines, Caco-2, HT-29, and SW 480, cells were treated with 3 different B. adolescentis isolates, and XTT assays were performed. B. adolescentis SPM0212 exhibited the highest efficacy (data not shown). To further characterize the functional substances of B. adolescentis SPM0212, the cell lines were treated with the butanol extract of this strain. The butanol extract significantly inhibited proliferation of both Caco-2 and SW480 cell lines, with inhibition of Caco-2 and SW480 growth by 70% and 40%, respectively, at 200 μg/ml (Figure 1). Treatment with the same concentration of butanol extract also decreased proliferation of HT-29, but there was no significant difference.

Figure 1
figure 1

Effects of growth inhibition by B. adolescentis SPM0212 on colon cancer cell lines (Caco-2, HT-29 and SW480). The cells (1 × 103 cells/well) were treated with B. adolescentis SPM0212 butanol extract (25, 50, 100, 200 μg/ml), and incubated for 72 hr at 37°C and 5.5% CO2. After adding 50 μl of the XTT labeling mixture, they were incubated for 6 hr at 37°C in 5.5% CO2. The absorbance was measured using an ELISA reader at 490 nm. The quantitative data were presented as means ± SD of three independent experiments. Control versus treatment groups, *p < 0.05; **p < 0.01.

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Effect of B. adolescentisSPM0212 on TNF-α and NO Production

Next, we examined the effects of B. adolescentis SPM0212 butanol extract on TNF-α and NO production by the macrophage RAW 264.7 cell line (Figure 2 and 3, respectively). B. adolescentis SPM0212 butanol extract significantly increased TNF-α production in a dose-dependent manner from 25 μg/ml to 200 μg/ml (Figure 2). Treatment with 200 μg/ml of butanol extract produced more TNF-α than LPS treatment, which was used as a positive control for macrophage activation. Treatment of RAW 264.7 cells with B. adolescentis SPM0212 butanol extract also increased production of NO (Figure 3). However, increases in TNF-α and NO production by B. adolescentis SPM0212 culture supernatant were not observed (data not shown).

Figure 2
figure 2

Effects of B. adolescentis SPM0212 on TNF-α production from RAW 264.7 cells. The cells (1 × 103 cells/well) were treated with LPS (100 ng/ml) or butanol extract of B. adolescentis SPM0212 (25, 50, 100, 200 μg/ml), and incubated for 48 hr at 37°C and 5.5% CO2. The extracellular levels of TNF-α in the culture media were determined by an ELISA reader at 450 nm. The quantitative data were presented as means ± SD of three independent experiments. Control versus B. adolescentis SPM0212 butanol extract, *p < 0.05; **p < 0.01.

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Figure 3
figure 3

Effects of B. adolescentis SPM0212 on NO production from RAW 264.7 cells. The cells (1 × 103 cells/well) were treated with LPS (50 ng/ml) or butanol extract of B. adolescentis SPM0212 (25, 50, 100, 200 μg/ml), and incubated for 22 hr at 37°C and 5.5% CO2. Nitrite concentrations in the culture media were determined using Griess reagent assay and measured by ELISA reader at 540 nm. The quantitative data were presented as means ± SD of three independent experiments. Control versus B. adolescentis SPM0212 butanol extract, *p < 0.05; **p < 0.01.

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Morphology of RAW 264.7 cells treated with B. adolescentisSPM0212

Normal RAW 264.7 cells, when cultured in medium alone, look refractile and rounded morphology and do not spread over the surface (Figure 4A). Activated macrophages usually display a distinct morphology, which is similar to the dendritic cell. Exposure to LPS (50 ng/ml; the positive control) induced morphological alteration of the RAW 264.7 cells (Figure 4B). Treatment with B. adolescentis SPM0212 butanol extract caused RAW 264.7 cells to become larger and rougher in a dose-dependent manner, suggesting activation (Figure 4C–F).

Figure 4
figure 4

Characterization of RAW 264.7 cells in response to butanol extract of B. adolescentis SPM0212. RAW 264.7 cells (1 × 104 cells/well) were cultured on cover slips in the presence of different concentrations of butanol extract of B. adolescentis SPM0212 for 48 hr. The cells were fixed and stained in Diff-quick and observed under a light microscope at 400×. (A) Murine macrophage cells. (B) LPS (50 ng/ml). (C) Butanol extract of B. adolescentis SPM0212 (25 μg/ml). (D) Butanol extract of B. adolescentis SPM0212 (50 μg/ml). (E) Butanol extract of B. adolescentis SPM0212 (100 μg/ml). (F) Butanol extract of B. adolescentis SPM0212 (200 μg/ml).

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Discussion

Bifidobacterium spp., and LAB, are probiotic organisms in humans and stimulate immune function and anti-tumor effects [19–45]. Although the precise mechanisms by which LAB inhibit colon cancer are not known, several have been proposed: (a) enhancing the host immune response, (b) binding and degrading potential carcinogens, (c) alterations in the intestinal microflora that produce putative carcinogens, (d) production of anti-tumorigenic or anti-mutagenic compounds in the colon, and (e) alteration of metabolic activities of intestinal microflora [18, 46–49].

LAB play an important role in the host immune system to produce anti-tumor effects [50–52]. Macrophages play a major role in the host defense against infection and tumor formation [53], and their function can be altered by a variety of stimulatory and suppressive signals and environmental factors. [54, 55]. The production of nitric oxide (NO) and tumor necrosis factors (TNF-α) by macrophages mediate killing and growth inhibition of tumor cells, bacteria, fungi, and parasites [56]. TNF-α is a non-glycosylated 17 kDa protein that exists as a trimer in solution, has receptors on almost all somatic cells, regulates immune modulation, and is cytotoxic to tumor cells [57, 58]. Also, TNF-α and reactive nitrogen intermediates play major roles in the in vitro anti-tumor activity of mouse peritoneal exudates from mice stimulated with wall peptidoglycan from B. infantis [32]. Therefore, cytokine production is a good measure of macrophage activation and further understanding of how Bifidobacterium affects the production of macrophage mediators may clarify how this strain affects immune function and tumor cells at the cellular level [22].

This study showed that the butanol extract of B. adolescentis SPM0212 increased secretion of TNF-α and NO from the macrophage RAW 264.7 cell line, as well as changed cell morphology. The butanol extract may contain key factors for increased macrophage activation and inhibition of tumor cell proliferation. Moreover, the butanol extract of B. adolescentis SPM0212 exerted direct anti-proliferative activity against three human colon cancer cell lines. We also observed that butanol extract of B. adolescentis SPM0212 – caused death of Caco-2, HT-29 and SW480 cells without any cytotoxicity to nonneoplastic epithelial cell (data not shown). Here, B. adolescentis SPM0212 potentiated TNF-production and may be beneficial in human intestinal tracts for immune reinforcement [59]. In contrast, most previously reported polysaccharides that exhibit anti-tumor activities did not directly inhibit the growth of tumor cells in vitro, but instead exerted anti-tumor activity by stimulating macrophages and immune responses. Therefore, the direct inhibitory effect by the butanol extract on tumor cell growth observed in this study is exceptional for polysaccharide biomaterials, but the active components remain to be elucidated. Further studies are needed to identify the effective components in the B. adolescentis SPM0212 butanol extract and will be required to clarify the precise mechanisms of this inhibition.

Conclusion

Bifidobacteria strains have health-promoting effects. Our results showed that the butanol extract of B. adolescentis SPM0212, isolated from fecal samples of healthy young Koreans, markedly and dose-dependently decreased the proliferation of three human colon cancer cell lines, Caco-2, HT-29, and SW480. In addition, the butanol extract increased the production of the macrophage mediators, TNF-α and NO, and changed macrophage RAW 264.7 cell morphology. Therefore, this extract could potentially help to enhance the host immune system and improve human health by helping to prevent colon cancer as a biological response modifier (BRM).

References

  1. Pisani P, Parkin DM, Ferlay J: Estimates of the worldwide mortality from eighteen major cancers in 1985. Int J Cancer. 1993, 54: 594-606. 10.1002/ijc.2910540413.

    Article  PubMed  Google Scholar 

  2. Lee WK, Lee SM: Inhibition effects of Lactic acid bacteria (LAB) on the Azoxymethance-induced colonic preneoplastic lesions. J Microbiol. 2000, 38: 169-175. [http://www.msk.or.kr/jsp/view_old_journalD.jsp?paperSeq=58]

    Google Scholar 

  3. Reddy BS: Nutritional factors and colon cancer. Crit Rev Fd Sci Nutr. 1995, 35: 175-190.

    Article  CAS  Google Scholar 

  4. Organisation for Economic Co-operation and Development (OECD): OECD Health Data: 2006.

  5. Milas L, Hunter B, Mason K, Grdina D, Withers H: Nonspecific immunotherapy of murine solid tumors with Corynebacterium granulosum. J Natl Cancer Inst. 1975, 54: 895-902.

    CAS  PubMed  Google Scholar 

  6. Proft T, Arcus VL, Handley V, Baker EN, Fraser JD: Immunological and biochemical characterization of streptococcal pyrogenic exotoxins I and J (SPE-I and SPE-J) from Streptococcus pyogenes. J Immunol. 2001, 166: 6711-6719.

    Article  CAS  PubMed  Google Scholar 

  7. Sato H, Yokosawa A, Arai H, Nagai H, Kumano N, Motomiya M, Konno K: Antitumor activity of hot-water extract form delipidated BCG. Tohoku J Exp Med. 1978, 125: 247-252.

    Article  CAS  PubMed  Google Scholar 

  8. Gilliland SE: Health and nutritional benefits of trom lactic acid bacteria. FEMS Microbiol Rev. 1990, 87: 175-188. 10.1111/j.1574-6968.1990.tb04887.x.

    Article  Google Scholar 

  9. Song MK, Woo SG, Jang JS, Kim JH, Kim HY, Hong SG, Lee BW, Park MH, Chung KS: Immunostiulating and anti-cancer effects of Pediococcus pentosaceus EROM101 isolated from Korea. Kor J Microbiol Biotechnol. 2003, 31: 355-361.

    Google Scholar 

  10. Bae HS, Shin MS, Kim YJ, Baek YJ: Effects of the lactic acid bacteria administration on fecal microflora and putrefactive metabolites in health adults. J Appl Microbiol Biotechnol. 1996, 24: 254-260.

    Google Scholar 

  11. Gismongo MR, Drago L, Lombardi A: Review of probiotics available to modify gastrointestinal flora. Int J Antimicrob Agents. 1999, 12: 287-292. 10.1016/S0924-8579(99)00050-3.

    Article  Google Scholar 

  12. Lee WK, Lee SM, Bae SH, Baek YJ: Effec of Bifidobacterium longum HY8001 administration on human fecal bacterial enzymes and microflora. J Appl Microbiol Biotechnol. 1999, 24: 267-272.

    Google Scholar 

  13. Mombelli B, Gismondo MR: The use of probiotics in medical practice. Int J Antimicrob Agents. 2000, 16: 531-536. 10.1016/S0924-8579(00)00322-8.

    Article  CAS  PubMed  Google Scholar 

  14. Parente E, Ricciardi A: Production, recovery and purification of bacteriocins from lactic acid bacteria. Appl Microbiol Biotechnol. 1999, 52: 628-638. 10.1007/s002530051570.

    Article  CAS  PubMed  Google Scholar 

  15. Penner R, Fedorak RN, Madsen KL: Probiotics and nutraceuticals: non-medicinal treatments of gastrointestinal diseases. Curr Opin Pharmacol. 2005, 5: 596-603. 10.1016/j.coph.2005.06.009.

    Article  CAS  PubMed  Google Scholar 

  16. Tahara T, Kanatani K: Isolation and partial characterization of cripacin A, a cell-associated bacteriocin produced by Lactobacillus cripatus JCM 2009. FEMS Microbiol Lett. 1997, 147: 287-290. 10.1111/j.1574-6968.1997.tb10255.x.

    Article  CAS  Google Scholar 

  17. Tahri K, Grill JP, Schneider F: Involvement of trihydroxy-conjugated bile salts in cholesterol assimilation by Bifidobacteria. Curr Microbiol. 1997, 34: 79-84. 10.1007/s002849900148.

    Article  CAS  PubMed  Google Scholar 

  18. You HJ, Oh DK, Ji GE: Anticancerogenic effect of a novel chiroinositol-containing polysaccharide from Bifidobacterium bifidum BGN4. FEMS Microbiol Lett. 2004, 240: 131-136. 10.1016/j.femsle.2004.09.020.

    Article  CAS  PubMed  Google Scholar 

  19. Arunachalam D, Gill HS, Chandra RK: Enhancement of natural immune function by dietary consumption of B. Lactis HN019. Eur J Clinc Nutr. 2000, 54: 263-267. 10.1038/sj.ejcn.1600938.

    Article  CAS  Google Scholar 

  20. Gill HS, Rutherfurd KJ, Cross ML, Gopal PK: Enhancement of Immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. Am J Clin Nutr. 2001, 74: 833-839.

    CAS  PubMed  Google Scholar 

  21. Gopal PK, Prasad J, Gill HS: Effects of the consumption of Bifidobacterium lactis HN019 (DR10â„¢) and galacto-oligosaccharides on the microflora of the gastrointestinal tract in human subjects. Nutr Res. 2003, 23: 1313-1328. 10.1016/S0271-5317(03)00134-9.

    Article  CAS  Google Scholar 

  22. Han S, Cho K, Lee CK, Song Y, Park SH, Ha NJ, Kim K: Enhancement of antigen presentation capability of dendritic cells and activation of macrophages by the components of Bifidobacterium pseudocatenulatum SPM1204. J Appl Pharmacol. 2005, 14: 174-180. [http://scholar.ndsl.kr/artdetail.do?cn=JAKO200508824145502&SITE=KLIC]

    Google Scholar 

  23. Miettinen M, Vuopio-Varkila J, Varkila K: Production of human tumor necrosis factor alpha, interleukin-6, and interleukin-10 is induced by lactic acid bacteria. Infect Immun. 1996, 64: 5403-5405.

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Park SH, Kim YA, Chung MJ, Kang BY, Ha NJ: Inhibition of proliferation by anti-microbial peptide isolated form Pediococcus pentosaceus and Lactobacillus spp. in colon cancer cell line (HT-29, SW480 and Caco-2). J Environ Toxicol. 2007, 22: 65-71.

    Google Scholar 

  25. Ayebo AD, Shahani KM, Dam R: Antitumor components of yogurt: fractionation. J Dairy Sci. 1981, 64: 2318-2323.

    Article  CAS  PubMed  Google Scholar 

  26. Esser P, Lend C, Clemmesen J: Antileukemic effects in mice from fermentation products of Lactobacillus bulgricus. Milchwissenschaft. 1983, 38: 257-260.

    Google Scholar 

  27. Kato I, Kobayashi S, Yokokura T, Mutai M: Antitumor activity of Lactobacillus casei in mice. Gann. 1981, 72: 517-523.

    CAS  PubMed  Google Scholar 

  28. Kim HY, Bae H, Baek YJ: In vivo antitumor effects of Lactic acid bacteria on sarcoma 180 and mouse lewis lung carcinoma. J Kor Cancer Assoc. 1991, 23: 188-197. [http://www.cancer.or.kr/journal/view.html?book=&start=0&scale=10&key=&key_word=&Vol=023&Num=02&year1=&year2=&sort=Publisher_date&aut_box=Y&sub_box=Y&sos_box=Y&key_box=Y&pub_box=Y&abs_box=&mod=vol&uid=2638]

    CAS  Google Scholar 

  29. Kim GT, Bae H, Baek YJ, Lee HY: Antitumor activity of Bifidobacterium adolesentis ATCC-15703 against sarcoma 180 in mice. Kor J Appl Microbiol Biotechnol. 1994, 22: 322-328.

    Google Scholar 

  30. Oda M, Hasegawa H, Komatsu S, Kambe M, Tsuchiya F: Anti-tumor polysaccharide from Lactobacillus spp. Agric Biol Chem. 1983, 47: 1623-1625.

    Article  CAS  Google Scholar 

  31. Reddy GV, Shahani KM, Banerjee MR: Inhibitory effect of yogurt on Ehrlich ascites tumor-cell proliferation. J Natl Cancer Inst. 1973, 50: 815-817.

    CAS  PubMed  Google Scholar 

  32. Sekine K, Ohta J, Onishi M, Tatsuki T, Shimokawa Y, Toida T, Kawashima T, Hashimoto Y: Analysis of antitumor properties of effector cells stimulated with a cell wall preparation (WPG) of Bifidobacterium infantis. Biol Pharm Bull. 1995, 18: 148-153.

    Article  CAS  PubMed  Google Scholar 

  33. Manjunath N, Ranganathan B: A cytotoxic substance produced by a wild culture of Lactobacillus casei D-34 against tumor cells. Indial J Exp Biol. 1989, 27: 141-145.

    CAS  Google Scholar 

  34. Goldin BR, Gorbach SL: Effect of Lactobacillus acidophilus dietary supplements in 1, 2-dimethylhydrazine dihydrochloride-induced intestinal cancer in rats. J Natl Cancer Inst. 1980, 64: 263-265.

    CAS  PubMed  Google Scholar 

  35. Goldin BR, Gualtieri LJ, Moore RP: The effect of Lactobacillus GG on the initiation and promotion of DMH-induced intestinal tumors in the rat. Nutr Cancer. 1996, 25: 197-204.

    Article  CAS  PubMed  Google Scholar 

  36. Kohwi T, Imai K, Tamura A, Hashimoto Y: Antitumor effect of Bifidobacterium infantis in mice. Gann. 1978, 69: 613-618.

    CAS  PubMed  Google Scholar 

  37. Rowland IR, Rumney CJ, Coutts JT, Lievense C: Effect of Bifidobaterium longum and inulin on gut bacterial metabolism and carcinogen-induce aberrant crypt foci in rats. Carcinogenesis. 1998, 19: 281-285. 10.1093/carcin/19.2.281.

    Article  CAS  PubMed  Google Scholar 

  38. Singh J, Rivenson A, Tomita M, Shimamura S, Ishibashi N, Reddy BS: Bifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Cacinogenesis. 1997, 18: 833-841. 10.1093/carcin/18.4.833.

    Article  CAS  Google Scholar 

  39. Commane D, Hughes R, Shortt C, Rowland I: The potential mechanisms involved in the anti-carcinogenic action of probiotics. Mut Res. 2005, 591: 276-289.

    Article  CAS  Google Scholar 

  40. Scardovi V: Genus Bifidobacterium. Bergey's Manual of Systemic Bacteriology. Edited by: Krieg NR, Holt JG. 1986, Williams & Willikins, MD, 2: 1418-1434.

    Google Scholar 

  41. Ahn JB: Isolation and characterization of Bifidobacterium producing exopolysaccharide. Food Eng Prog. 2005, 9: 291-296.

    Google Scholar 

  42. Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W: Trypan blue exclusion test of cell viability. Current Protocols in Immunoology. 1990, John Wiley and Sons, Greene Publishing Associates and Wiley-Interscience, New York, 2: A.3.3-

    Google Scholar 

  43. Gomez E, Melar MM, Silva GP, Portoles A, Gil I: Exocellular products from Bifidobacterium adolescentis as immunomodifiers in the lymphoproliferative responses of mouse splenocytes. FEMS Microbiol Lett. 1998, 56: 47-52. 10.1111/j.1574-6968.1998.tb13066.x.

    Article  Google Scholar 

  44. Kado-Oka Y, Fujiwara S, Hirota T: Effects of bifidobacteria cells on mitogenic response of splenocytes and several functions of phagocytes. Milchwissenshaft. 1991, 46: 626-630.

    Google Scholar 

  45. Lee J, Ametani A, Enomoto A, Sato Y, Motoshima H, Ike R, Kaminogawa S: Screening for the immunopotentiating activity of food microorganisms and enhancement of the immune response by Bifidobacterium adolescentis M101-4. Biosci Biotech Biochem. 1993, 57: 2127-2132.

    Article  CAS  Google Scholar 

  46. Hirayama D, Rafter J: The role of probiotic bacteria in cancer prevention. Microbes Infect. 2000, 2: 681-686. 10.1016/S1286-4579(00)00357-9.

    Article  CAS  PubMed  Google Scholar 

  47. Maclennan R, Jensen OM: Dietary fibre, transit time, fecal bacteria, steroids, and colon cancer in two Scandinavian population. Lancet. 1997, 30: 207-211.

    Google Scholar 

  48. Malhotra SL: Dietary factors in a study of colon cancer from Cancer Registry, with special reference to the role of saliva, milk and fermented milk products and vegetable fibre. Med Hypotheses. 1997, 3: 122-126. 10.1016/0306-9877(77)90024-X.

    Article  Google Scholar 

  49. Shahani KM, Ayebo AD: Role of dietary lactobacilli in gastrointestinal microecology. Am J Clin Nutr. 1980, 33: 2448-2457.

    CAS  PubMed  Google Scholar 

  50. de Simone C, Vesely R, Bianchi Salvadori B, Jirillo E: The role of probiotics in modulation of the immune system in man and in animals. Int J Immunother. 1993, 9: 23-28.

    Google Scholar 

  51. Kato I, Yokokura T, Mutai M: Macrophage activation by Lactobacillus casei in mice. Microbiol Immunol. 1983, 27: 611-618.

    Article  CAS  PubMed  Google Scholar 

  52. Schiffrin EJ, Rochat F, Link-Amster H: Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. J Dairy Sci. 1995, 78: 491-496.

    Article  CAS  PubMed  Google Scholar 

  53. Kovacsovics-Bankowski M, Clark K, Benacerraf B, Rock KL: Efficient major histocompatibility complex class I presentation of exogenous antigen upon phagocytosis by macrophages. Proc Natl Acad Sci USA. 1993, 90: 4942-4946. 10.1073/pnas.90.11.4942.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Abbas AK, Lichrman AH, Pobe JS: Cytokines. Cellular and Molecular Immunology. 1994, Saunders Co., Philadelphia, PA, 240 WB-second

    Google Scholar 

  55. Cavaillon JM: Cytokines and macrophages. Biomed Pharmacother. 1994, 48: 445-453. 10.1016/0753-3322(94)90005-1.

    Article  CAS  PubMed  Google Scholar 

  56. Lorsbach RB, Murphy WJ, Lowenstein CJ, Snyder SH, Russell SW: Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-gamma and lipopolysaccharide. J Biol Chem. 1993, 268: 1908-1913.

    CAS  PubMed  Google Scholar 

  57. Ashkenazi A, Dixit VM: Death receptors: signaling and modulation. Science. 1998, 287: 1305-1308. 10.1126/science.281.5381.1305.

    Article  Google Scholar 

  58. Natoli G, Costanzo A, Guido F, Moretti F, Lovreto M: Apoptotic, non-apoptotic and anti-apoptotic pathways of TNF signaling. Biochem Pharmacol. 1998, 56: 915-920. 10.1016/S0006-2952(98)00154-3.

    Article  CAS  PubMed  Google Scholar 

  59. Kim Y, Lee D, Kim D, Cho J, Yang J, Chung M, Kimm K, Ha N: Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by Bifidobacterium adolescentis SPM0212. Arch Pharm Res. 2008, 31: 468-473. 10.1007/s12272-001-1180-y.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This research was supported by the Sahmyook University Research Fund (2007). The authors are grateful to the Seoul Fellowship.

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Authors and Affiliations

  1. Department of Pharmacy, Sahmyook University, Seoul, 139-742, South Korea

    Do Kyung Lee, Seok Jang, Kyung Jae Kim & Nam Joo Ha

  2. Department of Life Science, Sahmyook University, Seoul, 139-742, South Korea

    Mi Jin Kim & Jung Hyun Kim

  3. Cellbiotech, Co. Ltd., Seoul, 157-030, South Korea

    Myung Jun Chung

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  1. Do Kyung Lee
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  3. Mi Jin Kim
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Correspondence to Nam Joo Ha.

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The authors declare that they have no competing interests.

Authors' contributions

This study was conceived by NJH and designed by NJH and KJK. NJH and MJC were responsible for obtaining funding and sample collection. MJK and JHK carried out the extraction and separation. The cultures, XTT, TNF-α, and NO assay, analysis of morphology were done by DKL and SJ. DKL performed data analysis and wrote the draft of the manuscript. All authors read and approved the final manuscript.

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Lee, D.K., Jang, S., Kim, M.J. et al. Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines. BMC Cancer 8, 310 (2008). https://doi.org/10.1186/1471-2407-8-310

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Keywords

BMC Cancer

ISSN: 1471-2407

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