Figure 3

Effects of B. adolescentis SPM0212 on NO production from RAW 264.7 cells. The cells (1 × 10³ cells/well) were treated with LPS (50 ng/ml) or butanol extract of B. adolescentis SPM0212 (25, 50, 100, 200 μg/ml), and incubated for 22 hr at 37°C and 5.5% CO₂. Nitrite concentrations in the culture media were determined using Griess reagent assay and measured by ELISA reader at 540 nm. The quantitative data were presented as means ± SD of three independent experiments. Control versus B. adolescentis SPM0212 butanol extract, *p < 0.05; **p < 0.01.