- Research article
- Open Access
- Open Peer Review
Evaluation of high-risk Human papillomaviruses type distribution in cervical cancer in Sichuan province of China
- En-qi Wu†1, 3,
- Guo-nan Zhang†3,
- Xiang-hui Yu1,
- Yuan Ren2,
- Ying Fan3,
- Yong-ge Wu1,
- Wei Kong1Email author and
- Xiao Zha2, 3Email author
© Wu et al; licensee BioMed Central Ltd. 2008
- Received: 06 March 2008
- Accepted: 22 July 2008
- Published: 22 July 2008
Infection with high-risk human papillomavirus is an important factor associated with cervical cancer, and the distribution of HPV types varies greatly worldwide. Determination of type-specific HPV prevalence constitutes an important step towards the development of vaccines for the prevention of cervical cancer.
The human papillomavirus (HPV) genotypes in 190 cervical cancer specimens taken from the Sichuan province, the most populous province of Southwest China, were detected by a combination of MY09/11 consensus primers PCR (MY09/11 PCR), type-specific primers one-step PCR (One-step TS PCR) and E6/E7 gene type-specific primers nested PCR (Nested TS PCR). The prevalence and distribution of HPV in patients with cervical cancer, especially for HPV types 16, 18, 52, 58 and 59, suspected to be most common in certain parts of China, was investigated.
The HPV infection rates detected by MY09/11 PCR, One-step TS PCR and Nested TS PCR were 159 (83.7%), 145 (76.3%) and 172 (90.5%), respectively. The overall HPV prevalence was 93.2% (177/190). The positive specimens for HPV16, 18, 52, 58 and 59 detected by One-step TS-PCR were 111 (58.4%), 14 (7.4%), 6 (3.2%), 13 (6.8%) and 4 (2.1%), respectively. By Nested TS-PCR analysis, the detection rates of HPV16, 52, 58 and 59 were increased to 140 (73.7%), 30 (15.8%), 37 (19.5%) and 25 (13.2%), while only 4 (2.1%) additional specimens were found to be infected with HPV18.
Our data demonstrate that, besides HPV 16, which was found to be the most prevalent type, HPV types 58, 52 and 59 are more prevalent than HPV18 in women with cervical cancer in the Sichuan area of China.
- Cervical Cancer
- Polymerase Chain Reaction Assay
- Nest Polymerase Chain Reaction
- Polymerase Chain Reaction Method
- Cervical Cancer Tissue
Cervical cancer is the second most common cancer in women; each year approximately 500,000 women worldwide are diagnosed with invasive cervical cancer and more than half of them die of this disease. Eighty percent of these deaths occur in developing countries. In China, there is an annual incidence of about 46,000 cases, and cervical cancer presents a major health problem . It is widely believed that persistent infection with high-risk human papillomavirus (HPV) represents the prime risk factor in cervical carcinogenesis [2, 3]. More than 100 different genotypes of HPV have been identified thus far, and there are approximately 40 known genital HPVs. At least 18 of these viruses have been associated with cervical cancer [4, 5]. Since the distribution of HPV genotypes in various geographical areas and populations varies widely [6–8], it is important to delineate the prevalence of different HPV types found in cervical cancer for guiding the selection of vaccine candidates and studying the oncogenic function of each genotype.
The most widely used method for HPV detection in cervical cancers is based on polymerase chain reaction (PCR) using either consensus or type-specific primers for the amplification of HPV DNA. The type-specific PCR primers have high specificity and sensitivity in the detection of certain HPV types, and the consensus PCR primers can detect a broad spectrum of HPV types in each reaction. Several investigators have introduced the nested PCR method for detection of HPV infection, using both consensus (MY09/MY11, GP5+/GP6+) and type-specific primers, and it has been found that the nested PCR assay is an extremely sensitive and reliable means for HPV detection because the viral DNA can be amplified with extremely low copy number [9–11].
It is well known that HPV16 and HPV18 are the most predominant high-risk types found in cervical cancer tissue in countries around the world, while the prevalence of the other high-risk HPV types varies from one region to another . The prevalence and type distribution of HPV types in cervical cancer in China is still uncertain. Huang et al.  found that HPV52 and 58 were prevalent in cervical cancers from Chinese women living in Shanghai, while Liu et al.  reported that HPV58 and 59 were found to be the third most common genotypes infected in women with cervical cancer from Guangzhou and Changsha, following HPV16 and 18. In addition, a multi-center study of HPV infection in cervical cancer including 5 geographical regions of China reported that HPV16, 18, 52 and 58 were the most common genotypes in China . These findings indicate that HPV types 16, 18, 52, 58 and 59 should be included in the evaluation of HPV detection assays when studying Chinese women.
In this study, MY09/11 PCR and One-step TS PCR assays were performed to evaluate the prevalence and distribution of high-risk HPV types. To describe the infections of HPV types 16, 18, 52, 58 and 59 in patients with cervical cancer in more detail, Nested TS PCR assays were performed for 190 cervical cancer specimens from the Sichuan province, the most populous province of southwest China, which covers an area of 480,000 square kilometers and has a population of about 90 million people.
The present study was carried out with the approval of the ethical committee of Sichuan Tumor Hospital, and patient consent was obtained for the collection of cervical cancer tissues. One hundred and ninety fresh cervical cancer tissues were obtained via surgical operation from patients from the Sichuan province between October 2004 and October 2006 and preserved at -70°C. The patients ranged in age from 17 to 71 years, with a mean of 46 years. Of the 190 tumor specimens, 176 were diagnosed as squamous-cell carcinoma (SCC), 12 as adenocarcinoma (AC) and 2 as SCC\AC mixed tumors.
DNA was extracted from frozen tissue specimens by DNeasy Tissue Kit (QIAGEN), according to the manufacturer's instructions. Extracted DNA was eluted with 100 μl AE buffer (10 mM Tris, pH 8.5) and stored at -20°C until it was analyzed. The quality of extracted DNA was checked by PCR amplification of β-globin gene (the primer forward 5'-CAACTTCATCCACGTTCACC-3' and reverse 5'-GAAGAGCCAAGGACAGGTAC-3') .
MY09/11 PCR assay
MY09/11 PCR was performed with all specimens in order to detect a broad spectrum of HPV genotypes, as described previously . The amplified DNA fragments were identified by electrophoresis in 1.5% agarose gel with ethidium bromide.
One-step TS PCR assay
Primers used for the detection of HPV DNA in cervical cancer tissues
Primers for the One-step TS PCR and the second round of the Nested TS PCR*
HPV16 E6 to E7 gene
HPV18 E6 gene
HPV52 E6 gene
HPV58 E6 gene
HPV59 E6 gene
Primers for the first round amplifications of Nested TS PCR**
HPV 16 URR to E7 gene
HPV 18 URR to E7 gene
HPV 52 URR to E7 gene
HPV58 URR to E7 gene
HPV59 URR to E7 gene
Nested TS PCR assay
To increase the sensitivity of the detection for HPV16, 18, 52, 58 and 59, the specimens negative by the One-step TS PCR for any genotype were further amplified by Nested TS PCR. The TS primers for HPV types 16, 18, 52, 58 and 59 of the first round of amplifications were designed specifically for this study to amplify a wider region (raging 619 to 662 bp) than those targeted by primers used in the One-step TS PCR assay, which were used in the second round of amplification of the Nested TS PCR (Table 1).
The first round reaction of nested PCR was performed under the following conditions: 40 cycles at 94°C for 1 min, 50°C for 1 min, and 72°C for 1 min. The first cycle was preceded by a 3 min denaturation step and the last cycle was followed by a 10 min extension step. The second round reaction of nested PCR was performed in 25 μl of reaction mixture, using 2 μl of PCR product from the first round reactions as template DNA. The reaction mixture contained 1×PCR buffer, 2.5 mmol/L MgCl2, 0.5 μmol/L of sense and anti-sense primer, 200 μmol/L of each deoxynucleoside, and 1 U Taq DNA polymerase (MBI, Fermentas). Specimens were amplified through 40 cycles at 95°C for 1 min, 55°C for 1 min, and 72°C for 1 min. This was followed by a final extension at 72°C for 5 min.
Five specimens, positive for HPV 16, 18, 52, 58 and 59 (as detected by One-step TS PCR and confirmed by sequencing), were used as positive controls. The reaction mixture, containing no DNA, was the negative control in PCR. When the nested PCR was performed, the product of the negative control in the first round was used as template in nested PCR amplification as negative controls to detect any contamination. The amplified DNA fragments were identified by electrophoresis in 1.5% agarose gel with ethidium bromide.
The MY09/11 PCR positive fractions of specimens which were negative for HPV16, 18, 52, 58 and 59 by One-step TS PCR assay were purified by PCR purification (QIAGEN) and sequenced by using BigDye Terminator v3.1 Cycle Sequencing Kit (PE Applied Biosystems) on an ABI 3730 Genetic Analyzer (PE Applied Biosystems). The HPV genotype was identified by comparing the sequence with that reported in GenBank using the BLAST 2.0 software server http://www.ncbi.nlm.nih.gov/blast.
McNemar's tests were performed to analyze statistical significance of the detection frequency data. Statistical analyses were performed with Statistical Analysis System software package (SPSS 13.0, SPSS, Chicago, IL).
HPV detection by the MY09/11 PCR, the One-step TS PCR and the Nested TS PCR in cervical cancer specimens (N = 190).
One-step TS PCR
One-step TS PCR + Nested TS PCR*
Positive rate (%)
Comparison of detection of HPV by E6 One-step TS PCR assay with detection by MY09/11 PCR assays for 190 specimens.
One-step TS PCR
Number and proportion of single and multiple infections in 190 specimens.
The overall HPV positive rate detected by a combination of MY09/11 PCR and One-step TS PCR was 86.8% (165/190), which is close to a meta-analysis of HPV prevalence among 5954 ICC cases in Asia (85.9%)  and slightly higher than the rate reported in a meta-analysis in China alone (83.7%) . HPV16 was the most common HPV type in this area, accounting for 58.4% of all cases, similar to the results of the meta-analysis in China (58.7%) . HPV18, with a detection rate (7.4%) lower than that reported in Asia (14.9%) , was the second most common type, followed by 58 (6.8%), 52 (3.2%), 45 (2.6%), 59 (2.1%), 31 (2.1%), 39 (1.1%), 33 (1.1%) and 82 (0.5%). Six (3.2%) specimens remained untyped, and three specimens were found to be infected by multiple types of HPV, including combinations of HPV16/HPV18, HPV16/HPV52 and HPV16/HPV59. The HPV positive rate detected by MY09/11 PCR (83.7%) was significantly higher (p = 0.009) than that detected by one-step TS PCR (76.3%). There were 20 specimens that were positive for HPV when using MY09/11 PCR but not One-step TS PCR as well as 6 specimens that had the reverse results. This discrepancy reflects the fact that these two methods vary in their abilities to detect HPV. The One-step TS PCR can determine specific HPV types due to its high specificity; however, this method requires a special pair of primers for detecting each type of HPV, and it is difficult to cover all HPV types that could be found in precancer and cervical cancer [6–8]. In this study, the One-step TS PCR assay was used only for five high-risk HPV types: 16, 18, 52, 58 and 59. Consensus primer PCR made it possible to detect various virus types simultaneously; however, because the amplified sequence of consensus primers is usually located in the L1 gene, which might be disrupted frequently as a consequence of HPV integration , the consensus primer mediated PCR method is not always suitable for detecting the integrated HPV in some cases. Therefore, consensus primer PCR has suboptimal sensitivity compared with the type-specific primer PCR methods.
The sensitivity and specificity of various HPV detection methods suggest a reason for the variance among analyses of the prevalence of HPV in cervical cancer in China. In order to characterize infections by HPV types 16, 18, 52, 58 and 59 in patients with cervical cancer in more detail, a reliable and sensitive detection system is necessary. Sotlar et al. have described a nested multiplex PCR (NMPCR) assay that combines degenerate E6/E7 consensus primers and type-specific primers for the detection and typing of most high risk human papillomavirus genotypes, and they suggest that the NMPCR method is a sensitive and useful tool for HPV DNA detection, especially when exact HPV genotyping and the identification of multiple HPV infections are required. However, in a pre-experiment using NMPCR, this method failed to detect the presence of multiple infections that identified by the One-step TS PCR method. These multi-infections were detectable if primers specific to only one HPV genotype were included in the second step of NMPCR (data not shown). This may be due in part to the fact that the multi-primers employed in the second step reaction of NMPCR included excessive off-target primers which caused considerable primer-dimer formation within pairs of primers or inefficient amplification . Therefore, the TS primers for HPV type 16, 18, 52, 58 and 59 for the first round of amplification of the Nested TS PCR assay were specifically designed for this study.
In this study, Nested TS PCR assays were performed to supplement One-step TS PCR and MY09/11 PCR assays for investigating the distribution of HPV genotypes in 190 cervical cancer specimens from China. The results demonstrate that improved sensitivity of the detection methods allows for increased findings of HPV16, 52, 58 and 59, but not HPV18. HPV58, 52 and 59 infections seem to be more prevalent than HPV18 in women with cervical cancer from the Sichuan area of China. The use of Nested TS PCR assays for detecting HPV16, 18, 52, 58 and 59 and for detecting the prevalence of other HPV genotypes deserves further investigation.
This work was supported in part by grants-in-aid 200504024 from the agency of science and technology of Jilin Province and by grants-in-aid 2006J13-133 from the agency of science and technology of Sichuan Province, China. The work also was partly supported by project 20674029 of National Natural Science Foundation of China (NSFC).
- Ferlay JBFPP, Parkin DM: GLOBOCAN 2002: Cancer Incidence, Mortality and Prevalence Worldwide. IARC Cancer Base No. 5, version 2.0. 2004, IARC PressGoogle Scholar
- Villa LL: Human papillomaviruses and cervical cancer. Advances in cancer research. 1997, 71: 321-341.View ArticlePubMedGoogle Scholar
- Ho GY, Burk RD, Klein S, Kadish AS, Chang CJ, Palan P, Basu J, Tachezy R, Lewis R, Romney S: Persistent genital human papillomavirus infection as a risk factor for persistent cervical dysplasia. Journal of the National Cancer Institute. 1995, 87 (18): 1365-1371.View ArticlePubMedGoogle Scholar
- de Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H: Classification of papillomaviruses. Virology. 2004, 324 (1): 17-27.View ArticlePubMedGoogle Scholar
- Munoz N, Bosch FX, de Sanjose S, Herrero R, Castellsague X, Shah KV, Snijders PJ, Meijer CJ: Epidemiologic classification of human papillomavirus types associated with cervical cancer. The New England journal of medicine. 2003, 348 (6): 518-527.View ArticlePubMedGoogle Scholar
- Clifford GM, Rana RK, Franceschi S, Smith JS, Gough G, Pimenta JM: Human papillomavirus genotype distribution in low-grade cervical lesions: comparison by geographic region and with cervical cancer. Cancer Epidemiol Biomarkers Prev. 2005, 14 (5): 1157-1164.View ArticlePubMedGoogle Scholar
- Clifford GM, Gallus S, Herrero R, Munoz N, Snijders PJ, Vaccarella S, Anh PT, Ferreccio C, Hieu NT, Matos E, Molano M, Rajkumar R, Ronco G, de Sanjose S, Shin HR, Sukvirach S, Thomas JO, Tunsakul S, Meijer CJ, Franceschi S: Worldwide distribution of human papillomavirus types in cytologically normal women in the International Agency for Research on Cancer HPV prevalence surveys: a pooled analysis. Lancet. 2005, 366 (9490): 991-998.View ArticlePubMedGoogle Scholar
- Bosch FX, Manos MM, Munoz N, Sherman M, Jansen AM, Peto J, Schiffman MH, Moreno V, Kurman R, Shah KV: Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group. J Natl Cancer Inst. 1995, 87 (11): 796-802.View ArticlePubMedGoogle Scholar
- Chow VT, Loh E, Yeo WM, Tan SY, Chan R: Identification of multiple genital HPV types and sequence variants by consensus and nested type-specific PCR coupled with cycle sequencing. Pathology. 2000, 32 (3): 204-208.View ArticlePubMedGoogle Scholar
- Fuessel Haws AL, He Q, Rady PL, Zhang L, Grady J, Hughes TK, Stisser K, Konig R, Tyring SK: Nested PCR with the PGMY09/11 and GP5(+)/6(+) primer sets improves detection of HPV DNA in cervical samples. Journal of virological methods. 2004, 122 (1): 87-93.View ArticlePubMedGoogle Scholar
- Kay P, Meehan K, Williamson AL: The use of nested polymerase chain reaction and restriction fragment length polymorphism for the detection and typing of mucosal human papillomaviruses in samples containing low copy numbers of viral DNA. Journal of virological methods. 2002, 105 (1): 159-170.View ArticlePubMedGoogle Scholar
- Huang S, Afonina I, Miller BA, Beckmann AM: Human papillomavirus types 52 and 58 are prevalent in cervical cancers from Chinese women. Int J Cancer. 1997, 70 (4): 408-411.View ArticlePubMedGoogle Scholar
- Liu J, Rose B, Huang X, Liao G, Carter J, Wu X, Thompson C: Comparative analysis of characteristics of women with cervical cancer in high-versus low-incidence regions. Gynecologic oncology. 2004, 94 (3): 803-810.View ArticlePubMedGoogle Scholar
- Lo KW, Wong YF, Chan MK, Li JC, Poon JS, Wang VW, Zhu SN, Zhang TM, He ZG, Wu QL, Li GD, Tam JS, Kahn T, Lam P, Cheung TH, Chung TK: Prevalence of human papillomavirus in cervical cancer: a multicenter study in China. Int J Cancer. 2002, 100 (3): 327-331.View ArticlePubMedGoogle Scholar
- Stephen AL, Thompson CH, Tattersall MH, Cossart YE, Rose BR: Analysis of mutations in the URR and E6/E7 oncogenes of HPV 16 cervical cancer isolates from central China. Int J Cancer. 2000, 86 (5): 695-701.View ArticlePubMedGoogle Scholar
- Manos MMTY, Wright DK: The use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cells. 1989, 7: 209-214.Google Scholar
- Sotlar K, Diemer D, Dethleffs A, Hack Y, Stubner A, Vollmer N, Menton S, Menton M, Dietz K, Wallwiener D, Kandolf R, Bultmann B: Detection and typing of human papillomavirus by e6 nested multiplex PCR. J Clin Microbiol. 2004, 42 (7): 3176-3184.View ArticlePubMedPubMed CentralGoogle Scholar
- Bao YP, Li N, Smith JS, Qiao YL: Human papillomavirus type distribution in women from Asia: a meta-analysis. Int J Gynecol Cancer. 2008, 18 (1): 71-79.View ArticlePubMedGoogle Scholar
- Bao YP, Li N, Smith JS, Qiao YL: Human papillomavirus type-distribution in the cervix of Chinese women: a meta-analysis. Int J STD AIDS. 2008, 19 (2): 106-111.View ArticlePubMedGoogle Scholar
- Park JS, Namkoong SE, Han SK, Nha DJ, Lee HY, Kim SJ: Comparison of L1 consensus primers with E6 type specific primers for detection of human papillomaviruses in paraffin sections of cervical neoplasia. Journal of Korean medical science. 1993, 8 (1): 60-67.View ArticlePubMedPubMed CentralGoogle Scholar
- Meuzelaar LS, Lancaster O, Pasche JP, Kopal G, Brookes AJ: MegaPlex PCR: a strategy for multiplex amplification. Nature methods. 2007, 4 (10): 835-837.View ArticlePubMedGoogle Scholar
- Smith JS, Lindsay L, Hoots B, Keys J, Franceschi S, Winer R, Clifford GM: Human papillomavirus type distribution in invasive cervical cancer and high-grade cervical lesions: a meta-analysis update. Int J Cancer. 2007, 121 (3): 621-632.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/8/202/prepub
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