Evaluation of high-risk Human papillomaviruses type distribution in cervical cancer in Sichuan province of China

BMC Cancer

Table 1 Primers used for the detection of HPV DNA in cervical cancer tissues

Name		Target DNA	Nucleotide sequence(5'-3')	Prime position	Product size(bp)
Primers for the One-step TS PCR and the second round of the Nested TS PCR*
1601	Sense	HPV16 E6 to E7 gene	CACAGTTATGCACAGAGCTGC	141–161	457
1602	Antisense		CATATATTCATGCAATGTAGGTGTA	597–573
1801	Sense	HPV18 E6 gene	CACTTCACTGCAAGACATAGA	170–-190	322
1802	Antisense		GTTGTGAAATCGTCGTTTTTCA	491–470
5201	Sense	HPV52 E6 gene	TAAGGCTGCAGTGTGTGCAG	178–197	229
5202	Antisense		CTAATAGTTATTTCACTTAATGGT	406–383
5801	Sense	HPV58 E6 gene	GTAAAGTGTGCTTACGATTGC	297–317	274
5802	Antisense		GTTGTTACAGGTTACACTTGT	570–550
5901	Sense	HPV59 E6 gene	CAAAGGGGAACTGCAAGAAAG	159–179	215
5902	Antisense		TATAACAGCGTATCAGCAGC	373–354
Primers for the first round amplifications of Nested TS PCR**
LF16	Sense	HPV 16 URR to E7 gene	AGGGCGTAACCGAAATCGGT	27–45	632
LR16	Antisense		CTGAGCTGTCATTTAATTGCTCA	658–636
LF18	Sense	HPV 18 URR to E7 gene	GGGAGTAACCGAAAACGGT	35–53	662
LR18	Antisense		TCCTCTGAGTCGCTTAATTGCTC	696–674
LF52	Sense	HPV 52 URR to E7 gene	GGGTGTAACCGAAAACGGT	31–49	619
LR52	Antisense		CTGAGCTGTCACCTAATTGCTCA	649–627
LF58	Sense	HPV58 URR to E7 gene	GGGTGTAACCGAAAACGGT	33–51	638
LR58	Antisense		CTGAGCTGTCACATAATTGCTCA	670–648
LF59	Sense	HPV59 URR to E7 gene	GTTAAGACCGAAAACGGTG	1–19	648
LR59	Antisense		GAGTCGGAGTCAGGTAATTGCTC	648–626

ISSN: 1471-2407