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A single dose of pegfilgrastim compared with daily filgrastim for supporting neutrophil recovery in patients treated for low-to-intermediate risk acute myeloid leukemia: results from a randomized, double-blind, phase 2 trial
© Sierra et al; licensee BioMed Central Ltd. 2008
Received: 04 December 2007
Accepted: 10 July 2008
Published: 10 July 2008
Patients with acute myeloid leukemia (AML) are often neutropenic as a result of their disease. Furthermore, these patients typically experience profound neutropenia following induction and/or consolidation chemotherapy and this may result in serious, potentially life-threatening, infection. This randomized, double-blind, phase 2 clinical trial compared the efficacy and tolerability of pegfilgrastim with filgrastim for assisting neutrophil recovery following induction and consolidation chemotherapy for de novo AML in patients with low-to-intermediate risk cytogenetics.
Patients (n = 84) received one or two courses of standard induction chemotherapy (idarubicin + cytarabine), followed by one course of consolidation therapy (high-dose cytarabine) if complete remission was achieved. They were randomized to receive either single-dose pegfilgrastim 6 mg or daily filgrastim 5 μg/kg, beginning 24 hours after induction and consolidation chemotherapy.
The median time to recovery from severe neutropenia was 22.0 days for both pegfilgrastim (n = 42) and filgrastim (n = 41) groups during Induction 1 (difference 0.0 days; 95% CI: -1.9 to 1.9). During Consolidation, recovery occurred after a median of 17.0 days for pegfilgrastim versus 16.5 days for filgrastim (difference 0.5 days; 95% CI: -1.1 to 2.1). Therapeutic pegfilgrastim serum concentrations were maintained throughout neutropenia. Pegfilgrastim was well tolerated, with an adverse event profile similar to that of filgrastim.
These data suggest no clinically meaningful difference between a single dose of pegfilgrastim and multiple daily doses of filgrastim for shortening the duration of severe neutropenia following chemotherapy in de novo AML patients with low-to-intermediate risk cytogenetics.
Acute myeloid leukemia (AML) is characterized by rapid proliferation of immature clonal myeloid cells, which leads to failure of normal hematopoiesis. Standard treatment for de novo AML consists of one or two cycles of intensive induction chemotherapy using cytarabine and anthracyclines with the aim of achieving complete remission. This is followed by one or more cycles of consolidation chemotherapy to maintain remission. Initial remission rates of 60% to 70% are typically achieved in patients aged less than 60 years [1, 2].
As a result of their disease, patients with AML are often neutropenic before beginning chemotherapy . Moreover, standard induction and consolidation regimens typically cause profound and protracted neutropenia, with a high attendant risk of infection and death [4, 5]. The duration of severe neutropenia is clinically significant, as it is closely correlated with development of infectious complications and associated morbidities .
Prophylactic use of myeloid growth factors reduces the severity and duration of neutropenia in patients receiving myelosuppressive chemotherapy [7–9], including patients treated for AML [4, 5, 10]. Pegylated filgrastim (pegfilgrastim) has the same mechanism of action as the first generation agent filgrastim, but has markedly reduced renal clearance, with neutrophil-mediated clearance being the major route of elimination. As a result, clearance of pegfilgrastim is decreased, and serum concentrations are sustained throughout the duration of neutropenia . A single subcutaneous (SC) dose of pegfilgrastim can provide neutrophil support for chemotherapy regimens with a range of myelosuppressive potential in patients treated for solid tumors [12–14] or lymphoma [15–17], and is as effective as daily SC doses of filgrastim [13, 14].
The aim of the current study was to compare the efficacy of pegfilgrastim with filgrastim for assisting neutrophil recovery in patients treated with standard induction and consolidation chemotherapy for AML.
Patients at least 18 years old with histologically confirmed de novo AML, Eastern Cooperative Oncology Group (ECOG) performance status ≤ 2, and life expectancy ≥ 3 months (with treatment) were eligible for study participation. Patients with French American British (FAB) subtype M3 or M7 were excluded. To avoid the inclusion of undetected secondary or postmyelodysplastic syndrome AML, high-risk (unfavorable) cytogenetic disease type AML (-5/del(5q), -7/del(7q), inv(3q), t(3;3), abn 11q23, 20q, 21q, t(6;9), t(9;22), abn 17p, complex karyotypes (≥ 3 abnormalities) [18, 19]) were also excluded. Patients with previously treated AML were not eligible for the study.
In this randomized, double-blind, multicenter, phase 2 study, patients received a course of standard IA 3+7 induction chemotherapy (idarubicin 12 mg/m2 days 1–3, cytarabine 100 mg/m2 twice daily days 1–7) (Induction 1), with a second course given, if necessary, after neutrophil recovery had occurred (Induction 2). If complete remission was achieved (≤ 5% myeloblasts), patients received one course of high-dose cytarabine consolidation therapy (3 g/m2 if aged <55 years, 2 g/m2 if aged ≥ 55 years or at increased risk for neurotoxicity; administered twice daily over 3 hours on days 1, 3, and 5).
During days 6 through 8 of Induction 1, patients were stratified by age (< or ≥ 55 years) and randomized (using an interactive voice response system [IVRS] and blinded treatment box number assignment) in a 1:1 ratio to receive either pegfilgrastim or filgrastim plus comparator-matched placebo. Separate computer-generated randomization lists were prepared for each age strata. The IVRS allocated the treatments in the order indicated in the list appropriate for the subject's age. Pegfilgrastim (Neulasta®; Amgen Inc., Thousand Oaks, CA, USA) was administered as a single SC 6-mg dose approximately 24 hours after completing chemotherapy. Filgrastim (Neupogen®; Amgen Inc., Thousand Oaks, CA, USA) 5 μg/kg SC was administered daily beginning 24 hours after chemotherapy and continuing until the post-nadir absolute neutrophil count (ANC) was ≥ 1.0 × 109/L for 3 consecutive days or ≥ 10 × 109/L for 1 day. Patients received the assigned treatment for all courses of chemotherapy. The total treatment duration was up to 3 months with a 1-month follow-up assessment. Patients randomized to active pegfilgrastim also received daily filgrastim-matched placebo injections until recovery of ANC in each cycle. Conversely, patients randomized to active daily filgrastim received a single pegfilgrastim-matched placebo injection in each cycle. Matched placebo vials were indistinguishable from those containing active agent. Both pegfilgrastim and filgrastim were formulated as clear, colorless, aqueous solutions. Placebo formulations contained the same excipients with no active agent.
The primary objective of the study was to compare time to recovery from severe neutropenia for the pegfilgrastim and filgrastim treatment groups. Other objectives were to compare the rate of complete remission following induction chemotherapy, ANC, adverse events, and the incidence and duration of hospitalization, fever, and intravenous anti-infective use.
In order to confirm that pegfilgrastim concentrations were maintained during prolonged neutropenia, a pharmacokinetic substudy was planned for the timepoint when 60 patients had completed Induction 1. The substudy was conducted by Amgen personnel (hematologist/oncologist, biostatistician, safety specialist, and medical affairs director) not associated with the study.
The study was conducted in accordance with the Declaration of Helsinki and with International Conference on Harmonization principles of good clinical practice. The appropriate independent ethics committees or institutional review boards reviewed and approved the protocol and informed consent forms. Written informed consent was obtained from all patients before study-specific procedures were performed.
Serum samples for determining pegfilgrastim concentrations were collected concurrently with complete blood count samples in Induction 1 (daily until ANC recovery occurred) and were analyzed using a validated enzyme-linked immunosorbent assay (ELISA). Pharmacokinetic parameters were estimated using noncompartmental analysis of serum concentration-time data.
Statistical methods were descriptive, with two-sided 95% confidence intervals (CI) for treatment differences calculated when appropriate. Time to recovery from severe neutropenia (ANC <0.5 × 109/L) was calculated from the first day of chemotherapy until the first of two consecutive post-nadir ANC values ≥ 0.5 × 109/L. Patients who did not develop severe neutropenia were considered recovered at day 1, and time to recovery was censored (at last ANC value) for patients who withdrew without recovery, started the next cycle before recovery, or did not recover. Summaries of time to neutrophil recovery were derived using Kaplan-Meier methods. Duration of febrile neutropenia (FN) (ANC <0.5 × 109/L and oral temperature ≥ 38.0°C) was calculated only in Induction 1 and defined as the onset time until the first of 2 consecutive days with resolution of both neutropenia and fever, or until the last day of the cycle if both parameters had not recovered. Duration of fever was counted from the first day with an oral temperature ≥ 38.0°C until the first of 2 consecutive days with temperature <38.0°C, or the last day of the cycle if not resolved.
The planned sample size of 120 patients (based on the width of the 95% CI around the difference between the median times to ANC recovery in the two groups) was sufficient to estimate treatment differences with acceptable accuracy. With 60 subjects per treatment group, the half-width of the 95% CI for the difference between treatment groups was estimated to be 2 to 3 days. On the basis of a previous study, it was calculated that the sample size would provide up to 70% power to provide a preliminary conclusion of noninferiority. The full analysis set included all randomized patients who received at least one dose of pegfilgrastim or filgrastim.
Patients and study conduct
Between March 2003 and April 2004, 84 patients from 27 investigational sites in Australia, Europe, and North America participated in the trial.
A planned interim analysis (after 60 patients had completed Induction 1) revealed an apparent difference in time to recovery from severe neutropenia between the two treatment groups. In the interests of patient safety, it was decided to suspend the study (ongoing patients discontinued study treatment and no new patients enrolled). However, subsequent review of the data by the study sponsor revealed a statistical programming error in the analysis of neutrophil recovery: the time to ANC recovery had been evaluated using a threshold ≥ 2.0 × 109/L instead of ≥ 0.5 × 109/L (the protocol-defined end point). As patients in the filgrastim arm received treatment until ANC was ≥ 1.0 × 109/L for 3 consecutive days, it was logical that they surpassed 2.0 × 109/L earlier than patients receiving pegfilgrastim. When this programming error was corrected, in the interim analysis the difference in time to ANC recovery between treatment groups was 0.5 days (95% CI: -1.8 to 2.8). This precision was within the boundaries set when the study was planned (2–3 days). After careful statistical consideration, it was decided that the study would not be restarted, as the potential bias introduced by halting the study prematurely was felt to be outweighed by the bias that would have been associated with restarting a partially unblinded study (note, no patients were unblinded). Moreover, the reduced sample size was large enough to estimate with adequate precision any differences between treatment groups with respect to the primary endpoint.
Demographics and disease characteristics
Pegfilgrastim 6 mg
Filgrastim 5 μg/kg/day
No. of Patients
Median (range), years
< 55 years, n (%)
≥ 55 years, n (%)
Male, n (%)
Baseline ANC, × 109/L
Most common FAB Type, n (%)
Cytogenetics, n (%)
Bone marrow cellularity, n (%)
Twenty-one patients (50%) in each treatment group completed the study. No significant differences between treatment groups were observed with respect to reasons for early withdrawal. Of 42 patients who were withdrawn prematurely, 15 were discontinued due to the early closure of the study. All but one of these patients (who never received randomized study medication) were included in all analyses, using censored values where appropriate. Only 6 patients (3 per group) had not recovered from severe neutropenia in Induction 1 at the time of study closure; these patients were permitted to receive open-label filgrastim treatment (2 patients in total received additional filgrastim therapy). The impact on the study of these early withdrawals at their relevant timepoints was evaluated in a sensitivity analysis and no effect was seen. In addition to those withdrawn because of study closure, an additional 27 patients were withdrawn from the study prematurely because of: failure to achieve complete remission (2 pegfilgrastim, 3 filgrastim), clinically significant delay (>14 days) in administration of consolidation chemotherapy following remission assessment (4, 1), adverse events (2, 2), death (1, 2), or other reasons (4, 6).
At least 90% of patients received full dose chemotherapy (>75% of protocol-specified dose) in Induction 1 and Consolidation. The median number of filgrastim doses administered was 16 during Induction 1 and 13 during Consolidation.
Time to recovery from severe neutropenia
ANC recovery in Induction 1 and Consolidation
Pegfilgrastim 6 mg (n = 42)
Filgrastim 5 μg/kg/day (n = 41)
Number of patients starting cycle
Number of patients (%) with SN
Number of patients (%) with ANC recoverya
Median time to ANC recoveryb
Difference between medians (95% CI)
0.0 (-1.9 to 1.9)
Number of patients starting cycle
Number of patients (%) with SN
Number of patients (%) with ANC recoverya
Median time to ANC recoveryb
Difference between medians (95% CI)
0.5 (-1.1 to 2.1)
During Consolidation, most patients had severe neutropenia, and again, ANC recovered in most cases (Table 2). Median time to ANC recovery was 17.0 days for pegfilgrastim versus 16.5 days for filgrastim (difference 0.5; 95% CI: -1.1 to 2.1 days). All 5 patients (4 pegfilgrastim, 1 filgrastim) who were classified as ANC recovery failures after Consolidation had a late ANC nadir after receiving additional off-study chemotherapy and were not followed up for sufficient time to document ANC recovery.
ANC profile and pegfilgrastim pharmacokinetics
Febrile neutropenia and fever
Thirty four patients (81%) in the pegfilgrastim group versus 36 patients (88%) in the filgrastim group developed FN, according to the protocol-specified definition, during Induction 1. The median duration (interquartile range) of FN during this phase was 15 (11, 20) days for the pegfilgrastim group, versus 14 (11.5, 18.5) days for the filgrastim group. The incidence and median (interquartile range) number of days with fever were similar in both treatment groups during Induction 1 (90%, 5 (3, 8) days for pegfilgrastim vs 93%, 6 (3, 12) days for filgrastim). During Consolidation, fever was reported in more patients in the pegfilgrastim group (17/22 (77%)) versus the filgrastim group (14/24 (58%)), but the median duration (interquartile range) was 2 (2, 3; 2, 2) days in each group.
Anti-infective use and hospitalization
Nonprophylactic anti-infectives were administered to all but two patients (both from the filgrastim group) during Induction 1; the median duration (interquartile range) of use was lower among pegfilgrastim (18.5 (13, 24) days) versus filgrastim recipients (21 (14, 27) days). During Consolidation, nonprophylactic anti-infective use was higher in the pegfilgrastim (82%) versus the filgrastim (67%) group. However, the median (interquartile range) duration of use was similar in the two groups (pegfilgrastim 21 (20, 33) vs filgrastim 21.5 (18, 35.5) days). The incidence and duration of hospitalization was similar in the two treatment groups, with nearly all patients being hospitalized, as per routine clinical practice.
All 83 patients had one or more adverse event. The pattern of events was consistent with that expected in a population receiving intensive chemotherapy for AML and was similar for the two treatment groups. Treatment-related adverse events occurred in 11 (26%) pegfilgrastim versus 9 (22%) filgrastim recipients. One of these events (vascular purpura; pegfilgrastim group) was classified as serious. The most frequently reported treatment-related adverse event was bone pain (pegfilgrastim 3 (7%) vs filgrastim 4 (10%)). Two patients (5%) withdrew from each treatment group as a result of adverse events. Three patients died during the study (1 pegfilgrastim, 2 filgrastim), all of respiratory complications associated with chemotherapy toxicity and baseline co-morbid conditions. None of the deaths were considered related to study drug.
This randomized, double-blind phase 2 study was the first to evaluate use of pegfilgrastim for neutrophil support in patients receiving chemotherapy for AML. Few studies on the use of pegfilgrastim in this setting have been published and none address the efficacy of pegfilgrastim in relation to filgrastim.
Treatment was anticipated to induce profound and protracted neutropenia with a high risk of FN. The efficacy and safety of filgrastim in this setting was previously demonstrated in a large (n = 521), randomized, double-blind trial, which showed significant reductions compared with placebo in the duration of severe neutropenia (P < 0.001) and fever, anti-infective use, and hospitalization . In the present study, we found no evidence to suggest a clinically meaningful difference between the efficacies of a single dose of pegfilgrastim 6 mg or daily filgrastim 5 μg/kg for reducing time to recovery from severe neutropenia – the primary endpoint. More than 80% of both treatment groups developed FN during Induction 1. FN was somewhat prolonged in this phase (median 15 days for pegfilgrastim vs 14 days for filgrastim), but this probably reflects the predefined stipulation for resolution of both fever and neutropenia before the event was considered resolved. Indeed, the duration of fever was much shorter than this (median 5 days pegfilgrastim vs 6 days filgrastim) and correlates well with data previously reported in this setting (7 days filgrastim vs 8.5 days placebo) .
Serum pegfilgrastim concentrations were sustained throughout the prolonged period of severe neutropenia and declined rapidly on ANC recovery. A positive correlation was observed between time to ANC recovery and time to pegfilgrastim concentration falling below the therapeutic threshold, further supporting the neutrophil-mediated clearance mechanism for pegfilgrastim. Pegfilgrastim was well tolerated and the adverse event profile was comparable to previously published data on filgrastim use in AML .
Initial complete remission rates in our study were similar between treatment groups and comparable to results from previous studies in this setting [4, 22–25]. Correlation between remission induction and improved survival was clearly demonstrated by Heil et al : median survival times for patients achieving versus not achieving complete remission were 18.2 versus 4.4 months. Concerns that hematopoietic growth factors might stimulate growth of the myeloid leukemic clone in patients have not been confirmed in clinical studies: to date, leukemic clone stimulation has been demonstrated only in vitro [26–28]. Long-term follow up (median 7 years) of patients in the Heil et al. study showed that filgrastim treatment did not have any adverse effects on complete remission or long-term survival rates . Since filgrastim and pegfilgrastim have the same active moiety, we would expect long-term outcomes for pegfilgrastim to be consistent with those for filgrastim.
Patients with unfavorable cytogenetics or secondary, relapsed, or previously treated AML were excluded from our study. It is known that neutrophil recovery may be delayed in such patients, and their exclusion was intended to minimize heterogeneity with respect to duration of severe neutropenia and thereby allow precise evaluation of the impact of the growth factors on neutrophil recovery time in the two treatment groups. The power of this study was weakened by early termination of recruitment and by patient withdrawals. The impact of study closure on the primary endpoint was balanced between treatment groups, as the same number of patients were withdrawn from both groups prior to recovery from severe neutropenia in Induction 1. This was a phase 2 trial and no specific definition of non-inferiority was planned, instead the study was designed to have sufficient subjects to estimate the difference between the groups in time to neutrophil recovery within ± 2–3 days, which was predefined as the period pertaining to a clinically relevant difference. Despite early termination of the study, which resulted in reduced patient numbers, the 95% CI observed (± 1.9 days) was narrower than that constituting a clinically relevant difference in neutrophil recovery between pegfilgrastim- and filgrastim-treated patients. Within this context, our findings provide no evidence of a clinically relevant difference between the treatment strategies. Data regarding secondary endpoints must, however, be interpreted within the limits of the revised sample size. Overall, given the paucity of data in this setting, the current study provides valuable information and an evidence base for the use pegfilgrastim in AML.
In conclusion, data from this phase 2 study suggest no difference in the efficacy of a single dose of pegfilgrastim compared with daily doses of filgrastim for reducing the duration of severe neutropenia in patients receiving induction and consolidation chemotherapy for low-to-intermediate risk AML.
We acknowledge the investigators, co-investigators, study coordinators, and patients who participated in the study at the following sites: Dr. Wolfgang R. Sperr, Wien, Austria; Dr. Werner Linkesch, Graz, Austria; Dr. Kerry Taylor, South Brisbane, Australia; Dr. Christopher Arthur, St. Leonards, Australia; Dr. Peter Bardy, Woodville South, Australia; Professor Bik To, Adelaide, Australia; Dr. Stéphane De Botton, Lille, France; Prof. Pierre Fenaux, Clichy, France; Prof. Gérard Nédéllec, Clamart, France; Dr. Anna Maria Nosari, Milano, Italy; Dr. Francesco Frassoni, Genova, Italy; Dr. Jordi Esteve, Barcelona, Spain; Dr. Jesús San Miguel, Salamanca, Spain; Dr. Antonio Torres, Córdoba, Spain; Dr. Javier Bueno, Barcelona, Spain; Dr. Sue Robinson, Halifax, Canada; Dr. Eric Bow, Winnipeg, Canada; Dr. S. Ronan Foley, Hamilton, Canada; Dr. Pierre Laneuville, Montreal, Canada. We also acknowledge the contributions of Dr. Bing-Bing Yang and Ms Anna Kido, Pharmacokinetics/Pharmacodynamics Department, Amgen Inc, and of Phillippa Barker, Clinical Development Department, Amgen Ltd, Cambridge, UK.
Study sponsored by Amgen Ltd, Cambridge, United Kingdom. Editorial support was provided by Nancy Graden and Julia Balfour of Amgen.
- Grimwade D, Walker H, Harrison G, Oliver F, Chatters S, Harrison CJ, Wheatley K, Burnett AK, Goldstone AH, Medical Research Council Adult Leukaemia Working Party: The predictive value of hierarchical cytogenetic classification in older adults with acute myeloid leukemia (AML): analysis of 1065 patients entered into the United Kingdom Medical Research Council AML11 trial. Blood. 2001, 98: 1312-1320. 10.1182/blood.V98.5.1312.View ArticlePubMedGoogle Scholar
- Jabbour EJ, Estey E, Kantarjian HM: Adult acute myeloid leukemia. Mayo Clin Proc. 2006, 81: 247-260.View ArticlePubMedGoogle Scholar
- Lowenberg B, Downing JR, Burnett A: Acute myeloid leukemia. N Engl J Med. 1999, 341: 1051-1062. 10.1056/NEJM199909303411407.View ArticlePubMedGoogle Scholar
- Heil G, Hoelzer D, Sanz MA, Lechner K, Liu Yin JA, Papa G, Noens L, Szer J, Ganser A, O'Brien C, Matcham J, Barge A: A randomized, double-blind, placebo-controlled, phase III study of filgrastim in remission induction and consolidation therapy for adults with de novo acute myeloid leukemia. The International Acute Myeloid Leukemia Study Group. Blood. 1997, 90: 4710-4718.PubMedGoogle Scholar
- Godwin JE, Kopecky KJ, Head DR, Willman CL, Leith CP, Hynes HE, Balcerzak SP, Appelbaum FR: A double-blind placebo-controlled trial of granulocyte colony-stimulating factor in elderly patients with previously untreated acute myeloid leukemia: A Southwest Oncology Group study (9031). Blood. 1998, 91: 3607-3615.PubMedGoogle Scholar
- Bodey GP, Buckley M, Sathe YS, Freireich EJ: Quantitative relationships between circulating leukocytes and infection in patients with acute leukemia. Ann Intern Med. 1966, 64: 328-340.View ArticlePubMedGoogle Scholar
- Lyman GH, Kuderer NM, Djulbegovic B: Prophylactic granulocyte colony-stimulating factor in patients receiving dose-intensive cancer chemotherapy: a meta-analysis. Am J Med. 2002, 112: 406-411. 10.1016/S0002-9343(02)01036-7.View ArticlePubMedGoogle Scholar
- Smith TJ, Khatcheressian J, Lyman GH, Ozer H, Armitage JO, Balducci L, Bennett CL, Cantor SB, Crawford J, Cross SJ, Demetri G, Desch CE, Pizzo PA, Schiffer CA, Schwartzberg L, Somerfeld MR, Somlo G, Wade JC, Wade JL, Winn RJ, Wozniak AJ, Wolff AC: 2006 Update of recommendations for the use of white blood cell growth factors: an evidence-based clinical practice guideline. J Clin Oncol. 2006, 24: 3187-3205. 10.1200/JCO.2006.06.4451.View ArticlePubMedGoogle Scholar
- Aapro MS, Cameron DA, Pettengell R, Bohlius J, Crawford J, Ellis M, Kearney N, Lyman G, Tjan-Heijnan VC, Walewski J, Weber DC, Zielinski C: EORTC guidelines for the use of granulocyte-colony stimulating factor to reduce the incidence of chemotherapy-induced febrile neutropenia in adult patients with lymphomas and solid tumours. Eur J Cancer. 2006, 42: 2433-2453. 10.1016/j.ejca.2006.05.002.View ArticlePubMedGoogle Scholar
- Harousseau JL, Witz B, Lioure B, Hunault-Berger M, Desablens B, Delain M, Guilhot F, Le Prise PY, Abgrall JF, Deconinck E, Guyotat D, Vilque JP, Casassus P, Tournilhac O, Audhuy B, Solary E: Granulocyte colony-stimulating factor after intensive consolidation chemotherapy in acute myeloid leukemia: results of a randomized trial of the Groupe Ouest-Est Leucemies Aigues Myeloblastiques. J Clin Oncol. 2000, 18: 780-787.PubMedGoogle Scholar
- Molineux G: The design and development of pegfilgrastim (PEG-rmetHuG-CSF, Neulasta). Curr Pharm Des. 2004, 10: 1235-1244. 10.2174/1381612043452613.View ArticlePubMedGoogle Scholar
- Vogel CL, Wojtukiewicz MZ, Carroll RR, Tjulandin SA, Barajas-Figueroa LJ, Wiens BL, Neumann TA, Schwartzberg LS: First and subsequent cycle use of pegfilgrastim prevents febrile neutropenia in patients with breast cancer: a multicenter, double-blind, placebo-controlled phase III study. J Clin Oncol. 2005, 23: 1178-1184. 10.1200/JCO.2005.09.102.View ArticlePubMedGoogle Scholar
- Green MD, Koelbl H, Baselga J, Galid A, Guillem V, Gascon P, Siena S, Lalisang RI, Samonigg H, Clemens MR, Zani V, Liang BC, Renwick J, Piccart MJ, International Pegfilgrastim 749 Study Group: A randomized double-blind multicenter phase III study of fixed-dose single-administration pegfilgrastim versus daily filgrastim in patients receiving myelosuppressive chemotherapy. Ann Oncol. 2003, 14: 29-35. 10.1093/annonc/mdg019.View ArticlePubMedGoogle Scholar
- Holmes FA, O'Shaughnessy JA, Vukelja S, Jones SE, Shogan J, Savin M, Glaspy J, Moore M, Meza L, Wiznitzer I, Neumann TA, Hill LR, Liang BC: Blinded, randomized, multicenter study to evaluate single administration pegfilgrastim once per cycle versus daily filgrastim as an adjunct to chemotherapy in patients with high-risk stage II or stage III/IV breast cancer. J Clin Oncol. 2002, 20: 727-731. 10.1200/JCO.20.3.727.View ArticlePubMedGoogle Scholar
- Younes A, Fayad L, Romaguera J, Pro B, Goy A, Wang M: Safety and efficacy of once-per-cycle pegfilgrastim in support of ABVD chemotherapy in patients with Hodgkin lymphoma. Eur J Cancer. 2006, 42: 2976-2981. 10.1016/j.ejca.2006.07.012.View ArticlePubMedGoogle Scholar
- Wolf M, Bentley M, Marlton P, Horvath N, Lewis ID, Spencer A, Herrmann R, Arthur C, Durrant S, van Kerkhoven M, MacMillan J, Mrongovius R: Pegfilgrastim to support CHOP-14 in elderly patients with non-Hodgkin's lymphoma. Leuk Lymphoma. 2006, 47: 2344-2350. 10.1080/10428190600881017.View ArticlePubMedGoogle Scholar
- Lopez A, Fernandez de Sevilla A, Castaigne S, Greil R, Sierra J, Sanchez J, Easton V, Bacon P: Pegfilgrastim Supports Delivery of CHOP-R Chemotherapy Administered Every 14 Days: A Randomised Phase II Study. Blood. 2004, 104: 3311-Google Scholar
- Byrd JC, Mrozek K, Dodge RK, Carroll AJ, Edwards CG, Arthur DC, Pettenati MJ, Patil SR, Rao KW, Watson MS, Koduru PR, Moore JO, Stone RM, Mayer RJ, Feldman EJ, Davey FR, Schiffer CA, Larson RA, Bloomfield CD: Pretreatment cytogenetic abnormalities are predictive of induction success, cumulative incidence of relapse, and overall survival in adult patients with de novo acute myeloid leukemia: results from Cancer and Leukemia Group B (CALGB 8461). Blood. 2002, 100: 4325-4336. 10.1182/blood-2002-03-0772.View ArticlePubMedGoogle Scholar
- Grimwade D, Walker H, Oliver F, Wheatley K, Harrison C, Harrison G, Rees J, Hann I, Stevens R, Burnett A, Goldstone A: The importance of diagnostic cytogenetics on outcome in AML: analysis of 1,612 patients entered into the MRC AML 10 trial. Blood. 1998, 92: 2322-2333.PubMedGoogle Scholar
- Cheson BD, Cassileth PA, Head DR, Schiffer CA, Bennett JM, Bloomfield CD, Brunning R, Gale RP, Grever MR, Keating MJ: Report of the National Cancer Institute-sponsored workshop on definitions of diagnosis and response in acute myeloid leukemia. J Clin Oncol. 1990, 8: 813-819.PubMedGoogle Scholar
- Yang BB, Kido A, Shibata A: Serum pegfilgrastim concentrations during recovery of absolute neutrophil counts in patients with cancer receiving pegfilgrastim after chemotherapy. Pharmacotherapy. 2007, 27: 1387-1393. 10.1592/phco.27.10.1387.View ArticlePubMedGoogle Scholar
- Hansen OP, Pedersen-Bjergaard J, Ellegaard J, Brincker H, Boesen AM, Christensen BE, Drivsholm A, Hippe E, Jans H, Jensen KB: Aclarubicin plus cytosine arabinoside versus daunorubicin plus cytosine arabinoside in previously untreated patients with acute myeloid leukemia: a Danish national phase III trial. The Danish Society of Hematology Study Group on AML. Leukemia. 1991, 5: 510-516.PubMedGoogle Scholar
- Wiernik PH, Banks PL, Case DC, Arlin ZA, Periman PO, Todd MB, Ritch PS, Enck RE, Weitberg AB: Cytarabine plus idarubicin or daunorubicin as induction and consolidation therapy for previously untreated adult patients with acute myeloid leukemia. Blood. 1992, 79: 313-319.PubMedGoogle Scholar
- Arlin Z, Case DC, Moore J, Wiernik P, Feldman E, Saletan S, Desai P, Sia L, Cartwright K: Randomized multicenter trial of cytosine arabinoside with mitoxantrone or daunorubicin in previously untreated adult patients with acute nonlymphocytic leukemia (ANLL). Lederle Cooperative Group. Leukemia. 1990, 4: 177-183.PubMedGoogle Scholar
- Berman E, Arlin ZA, Gaynor J, Miller W, Gee T, Kempin SJ, Mertelsmann R, Andreeff M, Reich L, Nahmias N: Comparative trial of cytarabine and thioguanine in combination with amsacrine or daunorubicin in patients with untreated acute nonlymphocytic leukemia: results of the L-16M protocol. Leukemia. 1989, 3: 115-121.PubMedGoogle Scholar
- Terpstra W, Lowenberg B: Application of myeloid growth factors in the treatment of acute myeloid leukemia. Leukemia. 1997, 11: 315-327. 10.1038/sj.leu.2400561.View ArticlePubMedGoogle Scholar
- Buchner T, Hiddemann W, Wormann B, Zuhlsdorf M, Rottmann R, Innig G, Maschmeier G, Ludwig WD, Sauerland MC, Heinecke A: Hematopoietic growth factors in acute myeloid leukemia: supportive and priming effects. Semin Oncol. 1997, 24: 124-131.PubMedGoogle Scholar
- Rowe JM, Liesveld JL: Hematopoietic growth factors in acute leukemia. Leukemia. 1997, 11: 328-341. 10.1038/sj.leu.2400592.View ArticlePubMedGoogle Scholar
- Heil G, Hoelzer D, Sanz MA, Lechner K, Noens L, Szer J, Ganser A, Matcham J, Renwick J, International Acute Myeloid Leukemia Study Group: Long-term survival data from a phase 3 study of filgrastim as an adjunct to chemotherapy in adults with de novo acute myeloid leukemia. Leukemia. 2006, 20: 404-409. 10.1038/sj.leu.2404090.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/8/195/prepub
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