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Diagnostic accuracy of diffuse reflectance imaging for early detection of pre-malignant and malignant changes in the oral cavity: a feasibility study
© Stephen et al.; licensee BioMed Central Ltd. 2013
Received: 28 November 2012
Accepted: 30 May 2013
Published: 5 June 2013
Diffusely reflected light is influenced by cytologic and morphologic changes that take place during tissue transformation, such as, nuclear changes, extracellular matrix structure and composition as well as blood flow. Albeit with varying degree of sensitivity and specificity, the properties of diffusely reflected light in discriminating a variety of oral lesions have been demonstrated by our group in multiple studies using point monitoring systems. However, the point monitoring system could not identify the region with the most malignant potential in a single sitting.
In order to scan the entire lesion, we developed a multi-spectral imaging camera system that records diffuse reflectance (DR) images of the oral lesion at 545 and 575 nm with white light illumination. The diagnostic accuracy of the system for 2-dimensional DR imaging of pre-malignant and malignant changes in the oral cavity was evaluated through a clinical study in 55 patients and 23 healthy volunteers. The DR imaging data were compared with gold standard tissue biopsy and histopathology results.
In total 106- normal/clinically healthy sites, 20- pre-malignant and 29- malignant (SCC) sites were compared. While the median pixel value of the R545/R575 image ratio for normal/clinically healthy tissue was 0.87 (IQR = 0.82-0.94), they were 1.35 (IQR = 1.13-1.67) and 2.44 (IQR = 1.78-3.80) for pre-malignant and malignant lesions, respectively. Area under the ROC curve to differentiate malignant from normal/clinically healthy [AUC = 0.99 (95% CI: 0.99-1.00)], pre-malignant from normal/clinically healthy [AUC = 0.94 (95% CI: 0.86-1.00)], malignant from pre-malignant [AUC = 0.84 (95% CI: 0.73-0.95)] and pre-malignant and malignant from normal/clinically healthy [AUC = 0.97 (95% CI: 0.94-1.00)] lesions were desirable.
We find DR imaging to be very effective as a screening tool in locating the potentially malignant areas of oral lesions with relatively good diagnostic accuracy while comparing it to the gold standard histopathology.
Oro-pharyngeal cancer is a major component of the global cancer burden [1, 2]. Early detection of pre-malignant changes facilitates timely adoption of preventive measures and treatment strategies . The potential beneficial effect of early detection and treatment may improve the survival rates in patients with cancer of the oral cavity . The gold standard for diagnosis of oral cavity cancer is an invasive tissue biopsy and histopathology examination. However, detection of the potentially malignant site for biopsy is visually challenging even for experienced clinicians, which often leads to multiple biopsies and delay in diagnosis.
Toluidine blue staining  and direct fluorescence visualization [6, 7] have been used in clinical settings as an adjunctive visual tool to enhance the contrast between the clinical lesions and the adjacent normal oral tissue. Diffusely reflected (DR) white light spectra were also studied by various groups [8, 9] for tissue differentiation in oral cavity. Several multi-centric clinical studies established the effectiveness of optical spectroscopy techniques for non-invasive detection of oral malignancies with good diagnostic accuracies [10–14]. However, they are point monitoring systems that analyse the tissue characteristics at a particular point in the entire area of an oral lesion.
Several spectroscopic imaging techniques that rely on tissue fluorescence have emerged recently for the detection of oral malignancies [6, 15–17]. Our previous experience suggests that the diagnostic accuracies based on DR spectroscopy are superior to fluorescence spectroscopy in point monitoring systems. We therefore developed a multi-spectral diffuse reflectance imaging system for recording of DR images in vivo and report the diagnostic accuracies of this system in comparison to the gold standard tissue biopsy and histopathology examination.
Diffuse reflectance imaging system
Study settings and study measurements
The present study was conducted at the Oral and Maxillofacial Pathology Department of the Government Dental College (GDC), Trivandrum, after obtaining the Ethical clearance from the GDC Ethics committee (No. IEC/C/28-A/2010/DCT), and strictly adhering to the approved protocols. Consecutive patients were invited to participate in the study. Before enrollment, the study subjects were asked to rinse their mouth with 0.9% saline solution. A clinician then examined the oral cavity of the patient for suspicious lesions, including white/red patches, mixed white and red lesions, non-healing ulcers and mucosal growth. The study procedures were explained to the patient and written informed consent was obtained from all participants before initiating any study related measurements. All patients enrolled were more than 20 years of age, and had no previous history of cancer or radiation/ chemotherapy treatment, or used any medication orally for the past seven days, and had no life threatening medical condition. Patient details were recorded and the clinical characteristics of the lesions were noted by a qualified clinician. There was no refusal to participate in the study. The DR imaging system was used to capture monochrome images of all suspected oral lesions at 545 and 575 nm. Tissue biopsies were taken from the region with maximum potential to show dysplastic characteristics in a lesion as identified by the PCM of the DR image ratio R545/R575. DR images recorded from 13 different anatomical sites of healthy volunteers were used as site-specific controls. Tissue biopsy was not taken for healthy volunteers and visual examination report of the pathologist was used for categorization of the tissue as clinically healthy.
The biopsy samples were fixed in 10% formalin and sent to the laboratory for pathological analysis. The tissue samples were then dehydrated in ascending grades of alcohol, embedded in paraffin block, micro-sectioned using microtome and mounted on a thin glass plate. This was then stained using haematoxylin and eosin stains. Two oral pathologists, who were blinded to the spectral measurement details, prepared the slides and provided the histo-pathological results independently. A third opinion was sought if there was any disagreement between the two independent reports.
The DR image ratio was further analyzed based on the mean pixel intensity of 100 pixels at 30 different points within the proposed biopsy site. Median value of the image ratio from these 30 points were determined and used for classifying the lesions into three groups, viz. normal/clinically healthy, pre-malignant or malignant (squamous cell carcinoma: SCC). Oral lesions were classified according to the image ratio value R545/R575 and the results were correlated with histo-pathologic findings to determine the diagnostic accuracies.
The characteristics of the study population are summarized as means and percentages. The DR spectral data were compared across different tissue types based on the histo-pathology findings using scatter plot. Receiver Operator Characteristics (ROC) curves were also constructed using the DR spectral data to differentiate pre-malignant from normal/clinically healthy tissue. The area under the ROC curves (AUC) and its 95% CI were computed. The statistical significance of AUC was tested using non-parametric assumptions.
Characteristics of study population categorized according to the grade of malignancy
Age (mean, SD)
Men (n, %)
Tobacco use (n, %)
Alcohol use (n, %)
Discordant findings of histopathology and DR imaging results
Histopathological (microscopic examination) report
DR image ratio result
Tumor epithelial cells were seen invading into the underlying connective tissue in the form of cords, islands, strands and nests. The invading cells exhibited features of pleomorphism, hyperchromasia, increased nuclear to cytoplasmic ratio and abnormal mitotic figures. Few keratin pearl formations were evident. Features suggestive of MDSCC.
Breach in the basement membrane was evident and tumor epithelial cells were seen invading into the underlying connective tissue in the form of islands, strands and nests. Keratin pearl formations were few. Tumor epithelial cells showed hyperchromasia, increased nuclear to cytoplasmic ratio, pleomorphism and abnormal mitotic figures. Features suggestive of MDSCC.
The epithelium was hyperchromatic in the basilar one-third region. Dense chronic inflammatory cells were seen within the connective tissue in the ulcerated region. Features suggestive of epithelial hyperplasia with mild dysplasia.
Epithelium exhibits no features of dysplasia. Moderate collections of chronic inflammatory cells were seen in the juxta epithelial region. Features suggestive of EHP with no dysplasia.
Ulcerated lesion with keratotic border
Epithelium exhibits features of hyperchromasia, increased mitotic figures, increased nuclear; cytoplasmic ratio, increased basilar hyperplasia involving two third of the epithelium. Chronic inflammatory cells were moderately dispersed in the juxta epithelial region. Features suggestive of EHP with moderate dysplasia.
Basal and suprabasal cells exhibited hyperplasia and hyperchromasia. The inflammatory cells were chronic and diffusely spread within the connective tissue. Features suggestive of EHP with mild dysplasia.
Diagnostic accuracies of DRIS in discriminating lesions
Area under the ROC curve
Malignant Vs Normal
Pre-malignant Vs Normal
Pre-malignant Vs malignant
Malignant & Pre-malignant Vs Normal
The present study examines the diagnostic accuracy of a spectral imaging system based on the principles of diffuse reflectance in discriminating healthy oral tissue from pre-malignant and malignant tissues. The diagnostic accuracy obtained in this study is superior to other imaging systems for detection of malignant changes in the oral cavity.
When light interacts with biological tissue a small portion of it is absorbed or transmitted while the rest undergoes multiple elastic scattering due to heterogeneity in the refractive index of the tissue components and gets diffusely reflected. Often, a portion of the impinging radiation is reflected from the surface when the roughness of the boundary is small in comparison with the wavelength of light. The portion that penetrates the sample gets scattered at a larger number of points in its path due to uneven, broken or bumpy boundary surfaces, where the coarseness is of the same order of magnitude as the wavelength. During tissue transformation healthy tissue undergoes morphometric and cytologic changes such as increase in epithelial thickness, nuclear size, nuclear to cytoplasmic ratio, changes in the chromatin texture and collagen content, and angiogenesis [20–22]. These changes modify the diffusely reflected component of the incoming radiation. We hypothesized that the diffuse reflectance intensity decreases during the transformation of tissue from healthy to dysplastic due to morphologic, cytologic and vascular changes associated with transformation. While our previous studies supported this hypothesis and differentiate pre-malignant and malignant tissue with relatively good sensitivity and specificity, the present study findings further strengthens this relationship and enables identification of areas with the most malignant potential in an oral lesion.
The diagnostic accuracies obtained in our study are superior to the diagnostic accuracies obtained in studies using direct tissue fluorescence visualization [6, 16, 17]. Unlike the present study, the discriminative capacity was tested in a homogenous group of malignant lesions consisting of mainly severe dysplastic tissues in all other studies. The high diagnostic accuracy obtained in our study underlines the potential use of this method in routine clinical practice. However, we could not perform site-specific analyses due to small number of cases in each anatomical location. The diagnostic accuracies presented in our study are therefore averaged for all types of lesions of the oral cavity and it would have been better if site-specific lesion classifications were possible.
The basis for our DR imaging method arises from the differences in the spectra obtained from the normal and diseased tissue due to the multiple physiological changes associated with tissue transformation from healthy to pre-malignant/dysplastic and malignant. The imaging method has the advantage of non-invasively scanning the entire lesion and its surrounding areas in real-time, and categorize oral lesions into normal/clinically healthy, pre-malignant and malignant tissue. Furthermore, it efficiently delineates the boundaries of neoplastic changes and locates the site with most malignant potential for a biopsy, thereby avoiding unnecessary repeated biopsies and delay in diagnosis. Imaging the entire region may also help the surgeons to identify the margins of the lesion that cannot be easily visualized by the naked eye during surgical interventions. Applications of digital image processing techniques may further enhance our ability to objectively identify and delineate the peripheral extent of neoplastic lesions.
Since clinical diagnosis based on DR imaging is possible in near real-time, there is practically no waiting period for the patient. The method is relatively cheap and can be implemented in all clinical settings with minimal training. Training the non-physician health workers in the imaging technique and screening the patients for tissue biopsy may further reduce the cost. However, the cost-effectiveness of mass screening of oral lesions using the imaging technique needs to be evaluated in different settings before its wider adaptation. Furthermore, the multi-spectral DR imaging technique presented in this paper has the potential to be used as an adjunct to colposcopy in the screening of cervical pre-cancers and in the identification the most malignant site for biopsy. Further studies in a larger population may help us to develop better classification algorithms for discriminating dysplastic lesions as mild, moderate and severe, and SCC lesions as ‘well differentiated’, ‘moderately differentiated’ and ‘poorly differentiated’ tissue categories.
Diffuse reflectance spectral imaging technique efficiently differentiates healthy tissue from pre-malignant and malignant tissue in the oral cavity. The imaging system developed for this study can be used as an adjunctive visual tool to enhance the contrast between dysplastic tissue and normal tissue in oral cavity lesions in clinical settings.
- Mignogna MD, Fedele S, Lo Russo L: The World Cancer Report and the burden of oral cancer. Eur J Cancer Prev. 2004, 13 (2): 139-142.View ArticlePubMedGoogle Scholar
- Saman DM: A review of the epidemiology of oral and pharyngeal carcinoma: update. Head Neck Oncol. 2012, 4: 1-10.1186/1758-3284-4-1.View ArticlePubMedPubMed CentralGoogle Scholar
- Carpenter WM, Silverman S: Oral cancer: the role of the dentist in prevention and early detection. Dent Today. 2001, 20 (5): 92-97.PubMedGoogle Scholar
- Sankaranarayanan R, Ramadas K, Thomas G, Muwonge R, Thara S, Mathew B, Rajan B: Effect of screening on oral cancer mortality in Kerala, India: a cluster-randomised controlled trial. Lancet. 2005, 365 (9475): 1927-1933. 10.1016/S0140-6736(05)66658-5.View ArticlePubMedGoogle Scholar
- Zhang L, Williams M, Poh CF, Laronde D, Epstein JB, Durham S, Nakamura H, Berean K, Hovan A, Le ND: Toluidine blue staining identifies high-risk primary oral premalignant lesions with poor outcome. Cancer Res. 2005, 65 (17): 8017-8021.PubMedGoogle Scholar
- Lane PM, Gilhuly T, Whitehead P, Zeng H, Poh CF, Ng S, Williams PM, Zhang L, Rosin MP, MacAulay CE: Simple device for the direct visualization of oral-cavity tissue fluorescence. J Biomed Opt. 2006, 11 (2): 024006-10.1117/1.2193157.View ArticlePubMedGoogle Scholar
- Poh CF, Ng SP, Williams PM, Zhang L, Laronde DM, Lane P, Macaulay C, Rosin MP: Direct fluorescence visualization of clinically occult high-risk oral premalignant disease using a simple hand-held device. Head Neck. 2007, 29 (1): 71-76. 10.1002/hed.20468.View ArticlePubMedGoogle Scholar
- Utzinger U, Brewer M, Silva E, Gershenson D, Blast RC, Follen M, Richards-Kortum R: Reflectance spectroscopy for in vivo characterization of ovarian tissue. Lasers Surg Med. 2001, 28 (1): 56-66. 10.1002/1096-9101(2001)28:1<56::AID-LSM1017>3.0.CO;2-L.View ArticlePubMedGoogle Scholar
- de Veld DC, Skurichina M, Witjes MJ, Duin RP, Sterenborg HJ, Roodenburg JL: Autofluorescence and diffuse reflectance spectroscopy for oral oncology. Lasers Surg Med. 2005, 36 (5): 356-364. 10.1002/lsm.20122.View ArticlePubMedGoogle Scholar
- Subhash N, Mallia JR, Thomas SS, Mathews A, Sebastian P, Madhavan J: Oral cancer detection using diffuse reflectance spectral ratio R540/R575 of oxygenated hemoglobin bands. J Biomed Opt. 2006, 11 (1): 014018-10.1117/1.2165184.View ArticlePubMedGoogle Scholar
- Jayanthi JL, Nisha GU, Manju S, Philip EK, Jeemon P, Baiju KV, Beena VT, Subhash N: Diffuse reflectance spectroscopy: diagnostic accuracy of a non-invasive screening technique for early detection of malignant changes in the oral cavity. BMJ Open. 2011, 1 (1): e000071-10.1136/bmjopen-2011-000071.View ArticlePubMedPubMed CentralGoogle Scholar
- Jayanthi JL, Subhash N, Stephen M, Philip EK, Beena VT: Comparative evaluation of the diagnostic performance of autofluorescence and diffuse reflectance in oral cancer detection: a clinical study. J Biophotonics. 2011, 4 (10): 696-706. 10.1002/jbio.201100037.View ArticlePubMedGoogle Scholar
- Mallia RJ, Narayanan S, Madhavan J, Sebastian P, Kumar R, Mathews A, Thomas G, Radhakrishnan J: Diffuse reflection spectroscopy: an alternative to autofluorescence spectroscopy in tongue cancer detection. Appl Spectrosc. 2010, 64 (4): 409-418. 10.1366/000370210791114347.View ArticlePubMedGoogle Scholar
- Mallia RJ, Subhash N, Mathews A, Kumar R, Thomas SS, Sebastian P, Madhavan J: Clinical grading of oral mucosa by curve-fitting of corrected autofluorescence using diffuse reflectance spectra. Head Neck. 2009, 32 (6): 763-779.Google Scholar
- De Veld DC, Witjes MJ, Sterenborg HJ, Roodenburg JL: The status of in vivo autofluorescence spectroscopy and imaging for oral oncology. Oral Oncol. 2005, 41 (2): 117-131. 10.1016/j.oraloncology.2004.07.007.View ArticlePubMedGoogle Scholar
- Roblyer D, Kurachi C, Stepanek V, Williams MD, El-Naggar AK, Lee JJ, Gillenwater AM, Richards-Kortum R: Objective detection and delineation of oral neoplasia using autofluorescence imaging. Cancer Prev Res (Phila). 2009, 2 (5): 423-431. 10.1158/1940-6207.CAPR-08-0229.View ArticleGoogle Scholar
- Rahman MS, Ingole N, Roblyer D, Stepanek V, Richards-Kortum R, Gillenwater A, Shastri S, Chaturvedi P: Evaluation of a low-cost, portable imaging system for early detection of oral cancer. Head Neck Oncol. 2010, 2: 10-10.1186/1758-3284-2-10.View ArticlePubMedPubMed CentralGoogle Scholar
- Subhash N, Jayanthi JL: A Novel Multi-Spectral Diffuse Reflectance Imaging Camera for Diagnosis of Oral Cavity Cancer. 2011, India: Controller General of Patents Designs and Trademarks, Government of India, Patent Number 2870/CHE/2011Google Scholar
- Jayanthi JL, Subhash N, Manju S, Nisha GU, Beena VT: Diffuse reflectance imaging: a tool for guided biopsy. Proc of SPIE. 2012, 8220: 1-9.Google Scholar
- Volynskaya Z, Haka AS, Bechtel KL, Fitzmaurice M, Shenk R, Wang N, Nazemi J, Dasari RR, Feld MS: Diagnosing breast cancer using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy. J Biomed Opt. 2008, 13 (2): 024012-10.1117/1.2909672.View ArticlePubMedGoogle Scholar
- Ferreira DS, Coutinho PG, Castanheira ES, Correia JH, Minas G: Fluorescence and diffuse reflectance spectroscopy for early cancer detection using a new strategy towards the development of a miniaturized system. Conf Proc IEEE Eng Med Biol Soc. 2010, 2010: 1210-1213.PubMedGoogle Scholar
- Mirabal YN, Chang SK, Atkinson EN, Malpica A, Follen M, Richards-Kortum R: Reflectance spectroscopy for in vivo detection of cervical precancer. J Biomed Opt. 2002, 7 (4): 587-594. 10.1117/1.1502675.View ArticlePubMedGoogle Scholar
- Kemmner W, Wan K, Ruttinger S, Ebert B, Macdonald R, Klamm U, Moesta KT: Silencing of human ferrochelatase causes abundant protoporphyrin-IX accumulation in colon cancer. FASEB J. 2008, 22 (2): 500-509.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/13/278/prepub
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