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UCN-01 induces S and G2/M cell cycle arrest through the p53/p21waf1or CHK2/CDC25C pathways and can suppress invasion in human hepatoma cell lines
© Wu et al.; licensee BioMed Central Ltd. 2013
Received: 21 November 2012
Accepted: 11 March 2013
Published: 28 March 2013
The Erratum to this article has been published in BMC Cancer 2014 14:216
UCN-01 (7-hydroxystaurosporine), a protein kinase inhibitor, has attracted a great deal of attention as a potent antitumour agent. Several clinical trials of UCN-01 alone or in combination with other agents for different tumour types are currently underway, and some of these trials have had positive results. Hepatocellular carcinoma has high incidence rates and is associated with poor prognosis and high mortality rates.
Three different hepatoma cell lines (Huh7, HepG2, and Hep3B) were treated with different concentrations of UCN-01, and the anti-tumour effects of UCN-01 were evaluated. Following UCN-01 treatment, cell growth was measured using an MTT assay, cell cycle arrest was assayed using flow cytometry, and the mechanisms of cell cycle arrest and invasion inhibition were investigated through western blotting and a Matrigel invasion assay.
After a 72-h UCN-01 treatment, the growth of different hepatoma cell lines was significantly inhibited in a dose-dependent manner, with IC50 values ranging from 69.76 to 222.74 nM. Flow cytometry results suggested that UCN-01 inhibits proliferation in the hepatoma cells by inducing S and G2/M phase arrest, but not G1/S arrest, which differs from previous reports that used other tumour cell lines. Western blot results illustrated that UCN-01 induces a G2/M phase arrest, regardless of the status of the p53/P21waf1 pathway, whereas the CHK2/CDC25C pathway and the p53/p21waf1 pathway were involved in the UCN-01-induced S phase arrest. UCN-01 remarkably inhibited Huh7 cell invasion in a time-dependent manner. Suppression of Huh7 cell invasion may be due to the down-regulation of phosphorylated β-catenin by UCN-01.
These findings suggest that UCN-01 induces hepatoma cell growth inhibition by regulating the p53/p21waf1 and CHK2/CDC25 pathways. Suppression of Huh7 cell invasion by UCN-01 may be due to the down-regulation of phosphorylated β-catenin. These data lend support for further studies on UCN-01 as a promising anti-HCC candidate.
Although substantial efforts have been made to improve the overall therapeutic outcomes for liver cancer, hepatocellular carcinoma (HCC) remains the fifth most common and the third most deadly cancer in the world . If HCC is detected early, surgical resection and liver transplantation are curative options. However, most patients have no symptoms until the disease is in an advanced stage, precluding surgical treatment [2, 3]. Many conventional anticancer treatments kill cells irrespective of whether they are normal or cancerous; therefore, patients suffer adverse side effects due to healthy cell loss. For this reason, new anticancer drugs are required.
UCN-01 (7-hydroxystaurosporine), both alone and in combination with chemotherapeutic agents and ionising radiation, is currently being evaluated in clinical trials as an antineoplastic agent . UCN-01 has antiproliferative activity and is well tolerated both in vitro and in vivo. This antiproliferative activity may be through protein kinase C inhibition . UCN-01 can enhance the cytotoxicity of chemotherapeutic agents through several potential mechanisms including inhibition of Chk1 . In addition, UCN-01 can abrogate cell cycle arrest independent of p53 and p21waf1[7–9].
Regulation of cell growth is mainly controlled through cell cycle control mechanisms. Many cytotoxic compounds and DNA damaging agents induce cell cycle arrest [10, 11]. In fact, many anti-cancer agents act by inducing cell cycle arrest. Progression of eukaryotic cells through the cell cycle is orchestrated by the sequential activation and inactivation of cyclin-dependent kinases (CDKs), which are associated with their respective cyclin subunits [12, 13]. In addition, cell cycle progression is regulated by the relative cellular concentrations of CDK inhibitors. The Cip/Kip family members include proteins, such as p21WAF1, that bind to cyclin/CDK complexes and prevent kinase activation, subsequently blocking cell cycle progression at G1 [14, 15]. In turn, activated Chk2 phosphorylates and inactivates the Cdc25c phosphatase, maintaining CDC2 in its phosphorylated-inactive form and leading to G2/M arrest [16, 17]. Furthermore, Cdc25c expression is strongly associated with metastatic disease in HCC patients. Cdc25c inhibitors inhibit HCC cell growth by specifically delaying progression through S phase, most likely because cyclin A is not induced, which results in decreased Cdk2 and Cdc2 kinase activities . The cyclin A was detected but the result was negative.
Although a number of studies have attempted to elucidate the mechanism of UCN-01 function, there have been no reports on the effects of UCN-01 on hepatoma cells. In this study, we focused on UCN-01-mediated inhibition of proliferation in 3 human hepatoma cell lines: Huh7 is mutant for P53 and defective for P21, Hep3B is P53 defective, and HepG2 expresses wild-type P53/P21. We examined which pathways the three cell lines have in common, and we performed invasion studies using Huh7 cells and attempted to illustrate the mechanisms at the protein level.
Chemicals and antibodies
UCN-01 was purchased from Sigma-Aldrich, Inc. (St. Louis, MO USA). Phospho-anti-P53 and phospho-T68-anti-Chk2 polyclonal antibodies were purchased from Cell Signaling Technology (Beverly, CA USA). Anti-Cdc25c, CDK2, Chk2, Cyclin B1, p53, and beta-actin monoclonal antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA USA). The anti-p21WAF1 monoclonal antibody was obtained from Calbiochem (Cambridge, MA USA).
Cell culture and UCN-01 treatments
The three different human HCC cell lines (HepG2, Hep3B, and Huh7) were grown at 37°C in the presence of 5% CO2 in Dulbecco's modified Eagle's media (DMEM) supplemented with 10% foetal bovine serum (FBS). Primary human hepatocytes were obtained from CellzDirect, Inc. (Tucson, AZ, USA) and cultivated in the manufacturer’s culture media. HCC cells and primary hepatocytes were treated with different concentrations of UCN-01 dissolved in dimethyl sulphoxide (DMSO). The cells were harvested, and whole cell extracts were used for western blots.
Growth inhibition assay
Cell proliferation was analysed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Briefly, the cells were seeded in a 96-well dish to a final concentration of 1×10  cells/well and incubated in DMEM containing 10% FCS overnight. After incubation with different concentrations of the tested compounds for 72 h, the cells were incubated for 2 h with DMEM containing 0.4 mg/ml MTT. The conversion of MTT to formazan in metabolically viable cells was measured at 490 nm absorbance in a 96-well microtiter plate reader. The per cent conversion in mock-treated control cells was used to evaluate the effects that the chemicals had on cell growth and to determine the IC50 concentration.
Cell cycle analysis
Exponentially growing cells were incubated with various concentrations of UCN-01 or treated with 0.1% DMSO as the vehicle control for 72 h. The cells were harvested, washed once with cold phosphate-buffered saline (PBS), fixed in ice-cold 80% ethanol, and stored at 4°C. Prior to analysis, the cells were washed again with PBS and suspended in 1 ml of a cold propidium iodide (PI) solution containing 10 μg/ml RNase A and 50 μg/ml PI. Next, flow cytometry was performed using the Beckman Coulter EPICS XL-MCL (Brea, US). The percentage of cells in various phases of the cell cycle was determined using FlowJo software (Ashland, US).
Matrigel invasion assay
Hepatoma cell invasion was assayed in a 6-well Biocoat Matrigel invasion chamber (8 μm; BD Biosciences, Franklin Lakes, NJ USA). Two hundred thousand cells were resuspended in 1 ml of serum-free DMEM and were added to the upper surface of the invasion chambers. Hepatocyte growth factor (HGF; Richmond, VA USA) at 10 ng/ml was used as the chemoattractant and placed in the lower wells. After 72 h, the cells on the upper surface of the membrane were removed using cotton swabs, and the filters were fixed by immersion in 4% formaldehyde for 10 min. After two washes with water, the invaded cells were stained with haematoxylin-eosin (Fisher Scientific, PA USA). Excess dye was removed by rinsing twice with water. The cells that had passed through the Matrigel matrix and the 8-μm pores in the culture inserts were counted using light microscopy.
Western blot analyses
The cells treated with various concentrations of UCN-01 were lysed with lysis buffer [20 mM Tris–HCl (pH 8.0), 150 mM NaCl, 2 mM EDTA, 100 mM NaF, 1% NP40, 1 μg /ml leupeptin, 1 l g/ml aprotinin, and 1 mM PMSF]. Cell lysate protein content was determined using a Bicinchoninic acid (BCA) protein assay kit (Pierce, Rockford, IL USA). The extracts (40 μg protein) were fractionated on polyacrylamide-SDS gels and transferred to PVDF membranes (Amersham, Arlington Heights, IL USA). The membranes were blocked with a solution containing 5% non-fat milk in PBS and incubated overnight with primary antibody at 4°C. Subsequently, the membranes were incubated with secondary antibody coupled to horseradish peroxidase (Amersham, Arlington Heights, IL USA). The reactive proteins were visualised using enhanced chemiluminescence (Amersham, Arlington Heights, IL USA) according to the manufacturer’s instructions. Western blot analyses were performed using standard procedures.
All values are expressed as the mean ± SD. The significance between mean values was evaluated using the two-tailed unpaired Student's t-test (P < 0.05).
Effects of UCN-01-mediated Growth Inhibition on Cell Lines
IC 50 values of the growth inhibitory effects mediated by UCN-01 in the Huh7, HepG2, and Hep3B cell lines
Cell cycle analysis by flow cytometry
UCN-01 induces cell cycle arrest in hepatoma cells via the p53/pP21waf1and CHK2/CDC25C pathways
Cell invasion is inhibited by UCN-01
Discussion and conclusion
In this study, we investigated UCN-01 antitumour activity in three different hepatoma cell lines with a particular emphasis on the mechanism of the G2/M cell cycle arrest induction and invasion inhibition in Huh7 cells. There were several novel findings presented here. (a) Growth of all three hepatoma cell lines was significantly inhibited by UCN-01 (each with a different sensitivity), whereas there was no effect on the normal hepatic cell line. (b) UCN-01-induced S and G2/M phase (and not G1) cell cycle arrest altered the p53 and CHK2 pathways. (c) Increased phosphorylation of Chk2 Thr68 and p53 was critical for UCN-01-induced G2 arrest, while total CHK2 and p53 remained the same. (d) In Huh7 cells, which are p53 mutant and p21 defective, and in Hep3B cells, which are p53 defective, S phase and G2/M cell cycle arrest is induced by UCN-01, suggesting that the G2/M arrest is p53 independent. (e) In UCN-01-treated Huh 7 cells, invasion activity was significantly inhibited, which may be correlated with the down-regulation of phosphorylated β-catenin at Ser 552.
UCN-01 was originally identified in Streptomyces as a selective protein kinase C inhibitor  and was subsequently found to inhibit many other kinases including cyclin-dependent kinase 2 (CDK2), Chk1, and, most recently, Akt [20–22]. By inhibiting Chk1, UCN-01 blocks the phosphorylation and proteosomal degradation of Cdc25c phosphatase [20, 23, 24]. Several phase I and II trials of UCN-01, either alone or in combination with established cytotoxic agents, are currently underway, and preliminary evidence of activity against certain malignancies has been reported [25–29].
There have been reports that UCN-01 inhibits the growth of various human cancers, e.g., leukaemia, colon, and pancreatic cancers, through the induction of a G1 arrest; [30–33] however, there are currently no reports on the effects UCN-01 has on HCC lines. In our study, UCN-01 effectively inhibited cell growth and viability in three human hepatoma cell lines in a dose-dependent manner. Cell cycle analysis revealed that UCN-01 inhibition of cell viability was caused by cell cycle arrest at the S and G2/M phases, accompanied by a decrease in the number of cells in G1. These results differ from the findings in other cancer studies [21, 31, 34]. In those studies, UCN-01-mediated induction of G1 arrest has been attributed to inhibition of CDK2, which results from CDK2 dephosphorylation at Thr160 and the induction of the CDK2-inhibiting proteins p21 and p27 in various cell lines [20, 35, 36].
Here, we focused on elucidating how UCN-01 induces G2/M arrest in Huh7, HepG2, and Hep3B cell lines. Among these cell lines, Huh7 is p53 mutant and p21 defective, and Hep3B is p53 defective, enabling us to determine whether the cell cycle arrest is p53 dependent.
The cyclin B/Cdk2 complex is critical in regulating the cell cycle transition from G2 to M phase and is controlled by phosphorylation at various sites . In our study, significant decreases in cyclin B and CDC25, as well as an increase in phosphorylated CHK2, were observed after 24 h of UCN-01 treatment. Cdc25 is a positive regulator of the cyclin B complex, which is inhibited by Chk2-mediated phosphorylation . Thus, UCN-01 may induce G2/M cell cycle arrest through the CHK2-CDC25-cyclin B pathway.
However, we found that UCN-01 induces p53 phosphorylation at Ser 15 and increased p21waf1 protein levels in the HepG2 cell line. As a crucial cell cycle regulator, the p53 tumour suppressor has an important role in the cellular response to platinum agents [38, 39]. For example, 1,2-diaminocyclohexane-acetato-Pt (DACH) arrests p53 wild-type cells in G1 and mutant p53 cells in G2/M phase in ovarian cancer . P53 transcriptionally activates a series of genes involved in both the G1-S and the G2/M transitions in response to genotoxic stress [41, 42]. Among these genes, p21 is a well-established negative regulator of the G1/S transition. p21 also inhibits the CDK1/cyclin B complex and maintains the G2 arrest [43–45]. Our study shows that the p53/p21waf1 pathway is also involved in the G2/M cell cycle arrest of HepG2 cells.
Interestingly, when UCN-01 was used to treat Huh7 cells (p53 mutant and p21 deficient) and Hep3B cells (p53 deficient), the G2/M arrest is still observed. These findings suggest that, although these two pathways both contribute to cell cycle arrest, the CHK2-Cdc25c pathway may have a more important role in these p53-deficient cell lines.
Finally, we found that Huh7 cell invasive activity was significantly inhibited by UCN-01. Recent studies indicate that phosphorylated β-catenin has a critical role in the invasion and development of many different tumours [46–49]. β-catenin was originally identified as a component of cell-cell adhesion structures, interacting with the cytoplasmic domain of E-cadherin and linking E-cadherin to α-catenin. Phosphorylation of β-catenin causes its dissociation from these cell-cell contacts, and β-catenin accumulates in both the cytosol and nucleus, enhancing its interaction with 14-3-3ζ via a binding motif containing Ser 552 . In our study, we examined total β-catenin and phosphorylated β-catenin levels at different time points, and found that phosphorylated β-catenin decreased 1–8 h after 100 nM UCN-01 treatment. Thus, inhibition of Huh7 cell invasion may be correlated with the down-regulation of phosphorylated β-catenin by UCN-01; however, further work is needed to confirm this finding.
Although recent studies indicate that UCN-01 binds to the human serum protein alpha1-acid glycoprotein and that this binding may hamper the ability of UCN-01 to inhibit cell proliferation and kill cells in vivo, UCN-01 has decent activity against several tumours at dose levels that are tolerated in clinical studies [27, 28]. These findings indicate that UCN-01 is an effective agent for tumour cell proliferation inhibition in vitro and in vivo and may be clinically applicable for HCC treatment in spite of its binding to serum proteins.In summary, our results show that UCN-01 inhibited HCC cell growth through the induction of S and G2/M phase arrest. UCN-01-induced G2/M cell cycle arrest involved molecular alterations of cell cycle regulatory proteins in human hepatoma cells. Increased Chk2 phosphorylation at Thr68 was critical for UCN-01-induced G2 arrest. These findings indicate that UCN-01 modulates the G2 transition through the p53-p21waf1 pathway as well as the Chk2–Cdc25c–Cdc2/cyclin B1 pathway. We also found that cell invasion was significantly inhibited by UCN-01 in Huh7 cells, which may be related to the down-regulation of phosphorylated β-catenin. Overall, the current study demonstrates that UCN-01 may be a potential drug candidate for the treatment of hepatocellular carcinomas.
This research is supported by National Science Foundation of China (81272642), the National Science Foundation for Young Scholars of China (81000674), Fundamental research funds for the central universities (10120110040) and Natural Science Foundation of Guangdong Province, China (S2011010004729).
- El-Serag HB, Rudolph KL: Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology. 2007, 132 (7): 2557-2576. 10.1053/j.gastro.2007.04.061.View ArticlePubMedGoogle Scholar
- Ercolani G, Grazi GL, Ravaioli M, Del GM, Gardini A, Cescon M, Varotti G, Cetta F, Cavallari A: Liver resection for hepatocellular carcinoma on cirrhosis: univariate and multivariate analysis of risk factors for intrahepatic recurrence. Ann Surg. 2003, 237 (4): 536-543.PubMedPubMed CentralGoogle Scholar
- Takayama T, Sekine T, Makuuchi M, Yamasaki S, Kosuge T, Yamamoto J, Shimada K, Sakamoto M, Hirohashi S, Ohashi Y: Adoptive immunotherapy to lower postsurgical recurrence rates of hepatocellular carcinoma: a randomised trial. Lancet. 2000, 356 (9232): 802-807. 10.1016/S0140-6736(00)02654-4.View ArticlePubMedGoogle Scholar
- Mow BM, Blajeski AL, Chandra J, Kaufmann SH: Apoptosis and the response to anticancer therapy. Curr Opin Oncol. 2001, 13 (6): 453-462. 10.1097/00001622-200111000-00007.View ArticlePubMedGoogle Scholar
- Mizuno K, Noda K, Ueda Y, Hanaki H, Saido TC, Ikuta T, Kuroki T, Tamaoki T, Hirai S, Osada S: UCN-01, an anti-tumor drug, is a selective inhibitor of the conventional PKC subfamily. FEBS Lett. 1995, 359 (2–3): 259-261.View ArticlePubMedGoogle Scholar
- Graves PR, Yu L, Schwarz JK, Gales J, Sausville EA, O'Connor PM, Piwnica-Worms H: The Chk1 protein kinase and the Cdc25C regulatory pathways are targets of the anticancer agent UCN-01. J Biol Chem. 2000, 275 (8): 5600-5605. 10.1074/jbc.275.8.5600.View ArticlePubMedGoogle Scholar
- Husain A, Yan XJ, Rosales N, Aghajanian C, Schwartz GK, Spriggs DR: UCN-01 in ovary cancer cells: effective as a single agent and in combination with cis-diamminedichloroplatinum(II)independent of P53 status. Clin Cancer Res. 1997, 3 (11): 2089-2097.PubMedGoogle Scholar
- Hirose Y, Berger MS, Pieper RO: Abrogation of the Chk1-mediated G(2) checkpoint pathway potentiates temozolomide-induced toxicity in a P53-independent manner in human glioblastoma cells. Cancer Res. 2001, 61 (15): 5843-5849.PubMedGoogle Scholar
- Levesque AA, Fanous AA, Poh A, Eastman A: Defective P53 signaling in P53 wild-type tumors attenuates P21waf1 induction and cyclin B repression rendering them sensitive to Chk1 inhibitors that abrogate DNA damage-induced S and G2 arrest. Mol Cancer Ther. 2008, 7 (2): 252-262.View ArticlePubMedGoogle Scholar
- Kaina B: DNA damage-triggered apoptosis: critical role of DNA repair, double-strand breaks, cell proliferation and signaling. Biochem Pharmacol. 2003, 66 (8): 1547-1554. 10.1016/S0006-2952(03)00510-0.View ArticlePubMedGoogle Scholar
- Nakanishi M, Shimada M, Niida H: Genetic instability in cancer cells by impaired cell cycle checkpoints. Cancer Sci. 2006, 97 (10): 984-989. 10.1111/j.1349-7006.2006.00289.x.View ArticlePubMedGoogle Scholar
- Koff A, Giordano A, Desai D, Yamashita K, Harper JW, Elledge S, Nishimoto T, Morgan DO, Franza BR, Roberts JM: Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science. 1992, 257 (5077): 1689-1694. 10.1126/science.1388288.View ArticlePubMedGoogle Scholar
- Morgan DO: Cyclin-dependent kinases: engines, clocks, and microprocessors. Annu Rev Cell Dev Biol. 1997, 13: 261-291. 10.1146/annurev.cellbio.13.1.261.View ArticlePubMedGoogle Scholar
- Elledge SJ, Harper JW: Cdk inhibitors: on the threshold of checkpoints and development. Curr Opin Cell Biol. 1994, 6 (6): 847-852. 10.1016/0955-0674(94)90055-8.View ArticlePubMedGoogle Scholar
- Sandal T: Molecular aspects of the mammalian cell cycle and cancer. Oncologist. 2002, 7 (1): 73-81. 10.1634/theoncologist.7-1-73.View ArticlePubMedGoogle Scholar
- Shiloh Y: ATM and related protein kinases: safeguarding genome integrity. Nat Rev Cancer. 2003, 3 (3): 155-168. 10.1038/nrc1011.View ArticlePubMedGoogle Scholar
- Abraham RT: Cell cycle checkpoint signaling through the ATM and ATR kinases. Genes Dev. 2001, 15 (17): 2177-2196. 10.1101/gad.914401.View ArticlePubMedGoogle Scholar
- Murphy D, Detjen KM, Welzel M, Wiedenmann B, Rosewicz S: Interferon-alpha delays S-phase progression in human hepatocellular carcinoma cells via inhibition of specific cyclin-dependent kinases. Hepatology. 2001, 33 (2): 346-356. 10.1053/jhep.2001.21749.View ArticlePubMedGoogle Scholar
- Takahashi I, Kobayashi E, Asano K, Yoshida M, Nakano H: UCN-01, a selective inhibitor of protein kinase C from Streptomyces. J Antibiot (Tokyo). 1987, 40 (12): 1782-1784. 10.7164/antibiotics.40.1782.View ArticleGoogle Scholar
- Akiyama T, Yoshida T, Tsujita T, Shimizu M, Mizukami T, Okabe M, Akinaga S: G1 phase accumulation induced by UCN-01 is associated with dephosphorylation of Rb and CDK2 proteins as well as induction of CDK inhibitor P21/Cip1/WAF1/Sdi1 in P53-mutated human epidermoid carcinoma A431 cells. Cancer Res. 1997, 57 (8): 1495-1501.PubMedGoogle Scholar
- Akiyama T, Sugiyama K, Shimizu M, Tamaoki T, Akinaga S: G1-checkpoint function including a cyclin-dependent kinase 2 regulatory pathway as potential determinant of 7-hydroxystaurosporine (UCN-01)-induced apoptosis and G1-phase accumulation. Jpn J Cancer Res. 1999, 90 (12): 1364-1372. 10.1111/j.1349-7006.1999.tb00721.x.View ArticlePubMedGoogle Scholar
- Akiyama T, Shimizu M, Okabe M, Tamaoki T, Akinaga S: Differential effects of UCN-01, staurosporine and CGP 41 251 on cell cycle progression and CDC2/cyclin B1 regulation in A431 cells synchronized at M phase by nocodazole. Anticancer Drugs. 1999, 10 (1): 67-78. 10.1097/00001813-199901000-00009.View ArticlePubMedGoogle Scholar
- Akinaga S, Nomura K, Gomi K, Okabe M: Enhancement of antitumor activity of mitomycin C in vitro and in vivo by UCN-01, a selective inhibitor of protein kinase C. Cancer Chemother Pharmacol. 1993, 32 (3): 183-189. 10.1007/BF00685833.View ArticlePubMedGoogle Scholar
- Wang Q, Fan S, Eastman A, Worland PJ, Sausville EA, O'Connor PM: UCN-01: a potent abrogator of G2 checkpoint function in cancer cells with disrupted P53. J Natl Cancer Inst. 1996, 88 (14): 956-965. 10.1093/jnci/88.14.956.View ArticlePubMedGoogle Scholar
- Varmark H, Kwak S, Theurkauf WE: A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells. Cell Cycle. 2010, 9 (2): 312-320. 10.4161/cc.9.2.10418.View ArticlePubMedGoogle Scholar
- Li T, Christensen SD, Frankel PH, Margolin KA, Agarwala SS, Luu T, Mack PC, Lara PJ, Gandara DR: A phase II study of cell cycle inhibitor UCN-01 in patients with metastatic melanoma: a California Cancer Consortium trial. Invest New Drugs. 2012, 30 (2): 741-748. 10.1007/s10637-010-9562-8.View ArticlePubMedGoogle Scholar
- Kummar S, Gutierrez ME, Gardner ER, Figg WD, Melillo G, Dancey J, Sausville EA, Conley BA, Murgo AJ, Doroshow JH: A phase I trial of UCN-01 and prednisone in patients with refractory solid tumors and lymphomas. Cancer Chemother Pharmacol. 2010, 65 (2): 383-389. 10.1007/s00280-009-1154-y.View ArticlePubMedGoogle Scholar
- Fracasso PM, Williams KJ, Chen RC, Picus J, Ma CX, Ellis MJ, Tan BR, Pluard TJ, Adkins DR, Naughton MJ: A Phase 1 study of UCN-01 in combination with irinotecan in patients with resistant solid tumor malignancies. Cancer Chemother Pharmacol. 2011, 67 (6): 1225-1237. 10.1007/s00280-010-1410-1.View ArticlePubMedGoogle Scholar
- Jimeno A, Rudek MA, Purcell T, Laheru DA, Messersmith WA, Dancey J, Carducci MA, Baker SD, Hidalgo M, Donehower RC: Phase I and pharmacokinetic study of UCN-01 in combination with irinotecan in patients with solid tumors. Cancer Chemother Pharmacol. 2008, 61 (3): 423-433. 10.1007/s00280-007-0485-9.View ArticlePubMedGoogle Scholar
- Bhonde MR, Hanski ML, Magrini R, Moorthy D, Muller A, Sausville EA, Kohno K, Wiegand P, Daniel PT, Zeitz M: The broad-range cyclin-dependent kinase inhibitor UCN-01 induces apoptosis in colon carcinoma cells through transcriptional suppression of the Bcl-x(L) protein. Oncogene. 2005, 24 (1): 148-156. 10.1038/sj.onc.1207842.View ArticlePubMedGoogle Scholar
- Abe S, Kubota T, Otani Y, Furukawa T, Watanabe M, Kumai K, Akiyama T, Akinaga S, Kitajima M: UCN-01 (7-hydoxystaurosporine) inhibits in vivo growth of human cancer cells through selective perturbation of G1 phase checkpoint machinery. Jpn J Cancer Res. 2001, 92 (5): 537-545. 10.1111/j.1349-7006.2001.tb01127.x.View ArticlePubMedGoogle Scholar
- Ullal AJ, Pisetsky DS: The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosis. Apoptosis. 2010, 15 (5): 586-596. 10.1007/s10495-010-0470-3.View ArticlePubMedPubMed CentralGoogle Scholar
- Blagosklonny MV, Dixon SC, Robey R, Figg WD: Resistance to growth inhibitory and apoptotic effects of phorbol ester and UCN-01 in aggressive cancer cell lines. Int J Oncol. 2001, 18 (4): 697-704.PubMedGoogle Scholar
- Shimizu E, Zhao MR, Nakanishi H, Yamamoto A, Yoshida S, Takada M, Ogura T, Sone S: Differing effects of staurosporine and UCN-01 on RB protein phosphorylation and expression of lung cancer cell lines. Oncology-Basel. 1996, 53 (6): 494-504.View ArticleGoogle Scholar
- Syljuasen RG, Sorensen CS, Hansen LT, Fugger K, Lundin C, Johansson F, Helleday T, Sehested M, Lukas J, Bartek J: Inhibition of human Chk1 causes increased initiation of DNA replication, phosphorylation of ATR targets, and DNA breakage. Mol Cell Biol. 2005, 25 (9): 3553-3562. 10.1128/MCB.25.9.3553-3562.2005.View ArticlePubMedPubMed CentralGoogle Scholar
- Usuda J, Saijo N, Fukuoka K, Fukumoto H, Kuh HJ, Nakamura T, Koh Y, Suzuki T, Koizumi F, Tamura T: Molecular determinants of UCN-01-induced growth inhibition in human lung cancer cells. Int J Cancer. 2000, 85 (2): 275-280.View ArticlePubMedGoogle Scholar
- Didier C, Cavelier C, Quaranta M, Galcera MO, Demur C, Laurent G, Manenti S, Ducommun B: G2/M checkpoint stringency is a key parameter in the sensitivity of AML cells to genotoxic stress. Oncogene. 2008, 27 (27): 3811-3820. 10.1038/sj.onc.1211041.View ArticlePubMedGoogle Scholar
- Steinmeyer K, Maacke H, Deppert W: Cell cycle control by P53 in normal (3T3) and chemically transformed (Meth A) mouse cells. I. Regulation of P53 expression. Oncogene. 1990, 5 (11): 1691-1699.PubMedGoogle Scholar
- Sturzbecher HW, Maimets T, Chumakov P, Brain R, Addison C, Simanis V, Rudge K, Philp R, Grimaldi M, Court W: P53 interacts with p34cdc2 in mammalian cells: implications for cell cycle control and oncogenesis. Oncogene. 1990, 5 (6): 781-795.Google Scholar
- McDonald AC, Brown R: Induction of P53-dependent and P53-independent cellular responses by topoisomerase 1 inhibitors. Br J Cancer. 1998, 78 (6): 745-751. 10.1038/bjc.1998.571.View ArticlePubMedPubMed CentralGoogle Scholar
- Baum N, Schiene-Fischer C, Frost M, Schumann M, Sabapathy K, Ohlenschlager O, Grosse F, Schlott B: The prolyl cis/trans isomerase cyclophilin 18 interacts with the tumor suppressor P53 and modifies its functions in cell cycle regulation and apoptosis. Oncogene. 2009, 28 (44): 3915-3925. 10.1038/onc.2009.248.View ArticlePubMedGoogle Scholar
- Sausville EA: Cell cycle regulatory kinase modulators: interim progress and issues. Curr Top Med Chem. 2005, 5 (12): 1109-1117. 10.2174/156802605774370874.View ArticlePubMedGoogle Scholar
- Kawabe T: G2 checkpoint abrogators as anticancer drugs. Mol Cancer Ther. 2004, 3 (4): 513-519.PubMedGoogle Scholar
- Vermeulen K, Van Bockstaele DR, Berneman ZN: The cell cycle: a review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif. 2003, 36 (3): 131-149. 10.1046/j.1365-2184.2003.00266.x.View ArticlePubMedGoogle Scholar
- Mani S, Wang C, Wu K, Francis R, Pestell R: Cyclin-dependent kinase inhibitors: novel anticancer agents. Expert Opin Investig Drugs. 2000, 9 (8): 1849-1870. 10.1517/135437220.127.116.119.View ArticlePubMedGoogle Scholar
- Fang D, Hawke D, Zheng Y, Xia Y, Meisenhelder J, Nika H, Mills GB, Kobayashi R, Hunter T, Lu Z: Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity. J Biol Chem. 2007, 282 (15): 11221-11229. 10.1074/jbc.M611871200.View ArticlePubMedPubMed CentralGoogle Scholar
- Hu J, Shao S, Song Y, Zhao J, Dong Y, Gong L, Yang P: Hepatocyte growth factor induces invasion and migration of ovarian cancer cells by decreasing the expression of E-cadherin, beta-catenin, and caveolin-1. Anat Rec (Hoboken). 2010, 293 (7): 1134-1139. 10.1002/ar.21147.View ArticleGoogle Scholar
- Du R, Xia L, Sun S, Lian Z, Zou X, Gao J, Xie H, Fan R, Song J, Li X: URG11 promotes gastric cancer growth and invasion by activation of beta-catenin signalling pathway. J Cell Mol Med. 2010, 14 (3): 621-635.PubMedGoogle Scholar
- Bandapalli OR, Dihlmann S, Helwa R, Macher-Goeppinger S, Weitz J, Schirmacher P, Brand K: Transcriptional activation of the beta-catenin gene at the invasion front of colorectal liver metastases. J Pathol. 2009, 218 (3): 370-379. 10.1002/path.2539.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/13/167/prepub
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