Measurement of hepatoma cell protein levels after UCN-01 treatment. Proteins were extracted and analysed through western blotting. β-actin was used as the loading control. (A) Western blots were performed for phospho-p53, p53, and p21waf1 in Huh7, HepG2, and Hep3B cells cultured with 0, 30, 100, and 300 nM UCN-01 for 24 h. (B) Total CHK2, phosphorylated CHK2, total cdc25a, and total cdc25c levels were detected. (C) Cyclin B was detected using a specific antibody. Cyclin B decreased in all three cell lines in a dose-dependent manner.