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The combination of gefitinib and RAD001 inhibits growth of HER2 overexpressing breast cancer cells and tumors irrespective of trastuzumab sensitivity
© Dragowska et al; licensee BioMed Central Ltd. 2011
Received: 6 July 2011
Accepted: 1 October 2011
Published: 1 October 2011
HER2-positive breast cancers exhibit high rates of innate and acquired resistance to trastuzumab (TZ), a HER2-directed antibody used as a first line treatment for this disease. TZ resistance may in part be mediated by frequent co-expression of EGFR and by sustained activation of the mammalian target of rapamycin (mTOR) pathway. Here, we assessed feasibility of combining the EGFR inhibitor gefitinib and the mTOR inhibitor everolimus (RAD001) for treating HER2 overexpressing breast cancers with different sensitivity to TZ.
The gefitinib and RAD001 combination was broadly evaluated in TZ sensitive (SKBR3 and MCF7-HER2) and TZ resistant (JIMT-1) breast cancer models. The effects on cell growth were measured in cell based assays using the fixed molar ratio design and the median effect principle. In vivo studies were performed in Rag2M mice bearing established tumors. Analysis of cell cycle, changes in targeted signaling pathways and tumor characteristics were conducted to assess gefitinib and RAD001 interactions.
The gefitinib and RAD001 combination inhibited cell growth in vitro in a synergistic fashion as defined by the Chou and Talalay median effect principle and increased tumor xenograft growth delay. The improvement in therapeutic efficacy by the combination was associated in vitro with cell line dependent increases in cytotoxicity and cytostasis while treatment in vivo promoted cytostasis. The most striking and consistent therapeutic effect of the combination was increased inhibition of the mTOR pathway (in vitro and in vivo) and EGFR signaling in vivo relative to the single drugs.
The gefitinib and RAD001 combination provides effective control over growth of HER2 overexpressing cells and tumors irrespective of the TZ sensitivity status.
HER2 overexpression is present in 13-30% of all breast cancers [1, 2] and it correlates with poor disease outcome, high rates of metastasis and resistance to conventional treatment modalities [1–5]. Trastuzumab (TZ; Herceptin®), a monoclonal antibody that targets the HER2 receptor and interferes with its function is effective in treating some HER2-positive breast cancers [6–8]. However, many patients with HER2-positive disease are insensitive to TZ both as first line treatment or following a relapse after conventional chemotherapy [6–9]. Furthermore, the majority of patients with metastatic disease that initially respond to TZ ultimately develop clinically relevant resistance to this agent [8, 9]. As TZ treatment has recently been expanded into the adjuvant setting , intrinsic and acquired resistance represents an important clinical problem that urgently awaits a discovery of novel drugs and development of innovative drug combinations to improve outcome for patients with advanced HER2-positive and TZ refractory disease.
Numerous studies have demonstrated that HER2 is often co-expressed in breast cancers with epidermal growth factor receptor (EGFR) [1, 5, 8, 11–16]. It has been established that dimerization of HER2 and EGFR generates a potent signaling response mediated primarily through activation of the phosphatidylinositol 3-kinase (PI3K)/AKT and the RAS-Raf-mitogen-activated protein kinase (MAPK) pathways that sustain cancer cell growth, proliferation and survival [5, 8]. Co-expression of EGFR and HER2 in breast cancer cell lines has been shown to induce drug resistance, including resistance to TZ [17, 18], and has been correlated with a negative prognosis for breast cancer patients [1, 11]. These data suggested that EGFR constitutes an important therapeutic target in breast cancers and have prompted investigators to consider gefitinib (ZD1839, Iressa®), a reversible small molecule inhibitor of the EGFR tyrosine kinase, for treatment of HER2 overexpressing and EGFR co-expressing breast malignancies .
The preclinical data have demonstrated that gefitinib exerts positive therapeutic effects in models of HER2 overexpressing breast cancer which have been attributed to blocking activity of the PI3K/AKT and the MAPK pathways, increased apoptosis, induction of cytostasis through G1/G0 cell cycle arrest and downregulation of cyclin D1, as well as inhibiting angiogenesis [12–14, 20, 21]. However, our previous study conducted in animals bearing HER2 overexpressing MCF7-HER2 and MDA-MB-435/LCC6-HER2 breast cancer xenografts showed that gefitinib monotherapy results in only modest reduction of tumor volume . The same study also showed that when gefitinib was used in combination with TZ the in vivo efficacy has been improved as judged by inhibition of tumor growth, but the data obtained by measuring multiple endpoints of therapeutic activity revealed that the combination was not beneficial . These results have been recapitulated in a clinical trial demonstrating that the TZ and gefitinib combination should not be used for treatment in patients with HER2-positive breast cancer .
More recently, it has been shown that HER2 overexpression in breast cancer is often associated with aberrant activation of the mTOR pathway [22, 23]. mTOR is a major cellular signaling hub that integrates inputs from the upstream signaling pathways, including tyrosine kinase receptors, while also governing energy homeostasis and cellular responses to stress such as nutrient deprivation and hypoxia [24, 25]. The mTOR kinase liaisons with either Raptor or Rictor proteins to form two functionally different complexes: rapamycin-sensitive mTOR complex 1 (mTORC1) and rapamycin-insensitive mTOR complex 2 (mTORC2) [24, 25]. The most prominent downstream effectors of mTORC1 include ribosomal S6 kinase (S6K) and the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) which regulate the translation of ribosomal and cap-dependent proteins essential for cell growth and G1 to S cell cycle progression [24, 25]. mTORC2 is an Akt Ser473 kinase that is controlled by a feedback inhibitory loop mediated through S6K1 (p70S6K) [24–29]. Because of its critical role in promoting cell growth, mTOR is considered an attractive target in cancer [25, 30]. Everolimus (RAD001) and CCI-779 are two allosteric mTORC1 inhibitors that are in clinical development for various malignancies; however, single-agent therapy has only modest efficacy in the metastatic breast cancer setting [31, 32]. These results have encouraged the investigation of mTORC1 inhibitors in combination with other targeted therapies such as aromatase inhibitors and HER2 targeting drugs. A Phase I/II trial of RAD001 in combination with TZ in refractory HER2 positive metastatic breast cancer have reported encouraging results with 34% of patients achieving clinical benefit . Interestingly, several preclinical studies documented that mTOR inhibitors combined with EGFR targeted agents increase efficacy of treatment in renal, lung, pancreatic, colon, prostate and HER2-negative breast cancer models [34–36]. However, the therapeutic effects of EGFR and mTOR inhibitors in combination have not yet been broadly assessed in HER2 overexpressing breast cancers with different TZ sensitivity. Here, we show that the EGFR inhibitor gefitinib and the mTOR inhibitor RAD001 when used in combination improve effectiveness of the treatment in HER2 overexpressing breast cancers that results in impediment of cancer growth.
Cells, tumor xenografts and treatments
MCF7-HER2 cells were a gift from Dr. M. Alaoui-Jamali (McGill University, Montreal, Quebec, Canada) , SKBR3 cells were purchased from American Type Culture Collection (ATCC) and JIMT-1 cells  were purchased from German Collection of Microorganisms and Cell Culture (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ). All cell lines were tested Mycoplasma negative by PCR reaction. MCF7-HER2 cells were maintained in RPMI, SKBR-3 in McCoy's 5A and JIMT-1 in DMEM supplemented with L-glutamine and 10% fetal bovine serum. For in vivo studies JIMT-1 and MCF7-HER2 cells were harvested in the exponential growth phase and 5 × 106 (JIMT-1) or 1 × 107 (MCF7-HER2) cells were injected subcutaneously (s.c.) on the back of female Rag2M immuno-compromised mice. Mice receiving MCF7-HER2 cells were implanted with 17-β-estradiol 60-day release tablets (IRA, Sarasota, FL) one day prior to tumor inoculation. Tumor growth was monitored twice a week; tumor sizes were calculated using the formula: 0.5 [length (mm)] × [width (mm)2]. All agents were delivered as oral gavage. Treatment was initiated on day 17 and carried-out Monday through Friday (QDx5) for 28 (JIMT-1) or 25 (MCF7-HER2) days. RAD001 (a generous gift from Novartis) was diluted with vehicle (Novartis, content not disclosed) and aliquots were kept frozen for the course of treatment. RAD001 and vehicle aliquots were thawed 10-30 min before dosing animals and unused portions were discarded. Gefitinib (a generous gift from AstraZeneca) was solubilized in 0.5% Tween-80 in sterile milli-Q water (vehicle) and kept at 4°C. Gefitinib formulation was prepared weekly. Combination treated mice were dosed first with gefitinib followed by RAD001 four hours later. Tumors were harvested 30 min after the last dose and cut into two parts: one part was frozen in liquid nitrogen (N2) for Western blot analysis and the second part was frozen in embedding medium and stored in -80°C for immunohistochemical processing. Animal protocols were approved by the University of British Columbia Animal Care Committee, and these studies were done in accordance with guidelines established by the Canadian Council on Animal Care.
Alamar Blue and IN Cell 1000 screening assays
Cells were plated under standard serum conditions (10% FBS) in their respective media in triplicate wells/condition in 96-well flat bottom plates (Optilux, Falcon, Becton-Dickinson). MCF7-HER2 and JIMT-1 cells were plated at densities of 5,000 or 1,500 cells/well for 72 and 144 h drug incubations, respectively. SKBR3 cells were plated at 15,000 and 4000 cells/well for 72 and 144 h, respectively. Cells were allowed to adhere overnight. Next day the cells were treated with gefitinib, RAD001 and combination of both drugs at a fixed molar ratio over a broad dose range to establish growth curves for a 72 h and 144 h read-out. Stock solutions of 20 mM gefitinib and 20 mM RAD001 were prepared in DMSO and stored in -80°C. Gefitinib and RAD001 stocks were diluted in medium with decreasing percentage of DMSO and 10× concentrated drugs were added to cells. The final concentration of DMSO in vehicle and drug treated cells was standardized to 0.5% (vol/vol) and the final media volume in 96-well plate wells was 200 μl. After 72 or 144 h incubation, Alamar Blue (Invitrogen, Burlington, ON, CA) was added to one set of plates to evaluate cell viability. Fluorescence was measured using the FLUOstar OPTIMA plate reader (BMG Labtechnologies, Germany) with 544 nm excitation and 590 nm emission filters. A second set of plates was stained with DRAQ5 (Biostatus, Shepshed, UK) and ethidium homodimer (ETH; Molecular Probes, Invitrogen) followed by imaging with IN Cell 1000 Analyzer (GE Healthcare). Ten images per well were acquired with 10× objective. Data analysis strategies were supported by enterprise level servers. Images were analyzed with IN Cell 1000 Investigator software using the Multi Target Analysis (MTA) module and data were reported as the percentage of dead cells normalized to vehicle control by subtracting the percentage of dead cells in the DMSO control from the percentage of dead cells in treated cultures.
Following drug treatment in vitro, the number of viable cells was measured using the Alamar Blue assay as described above. Alamar Blue measures mitochondrial activity which is lost upon cell death. The data obtained with the Alamar Blue assay were normalized to the vehicle control and expressed as % viability. Next, these data were converted to Fraction affected (Fa; range 0-1), where Fa = 0 represents 100% viability and Fa = 1 represents 0% viability) and analyzed with the CompuSyn™ program (Biosoft, Ferguson, MO) based upon the Chou and Talalay median effect principle . This program calculates a combination index (CI) that is used to identify synergistic, additive, and antagonistic drug interactions.
Cells were plated in their respective media containing 10% FBS in T25 flasks or 6 cm diameter culture dishes and allowed to adhere overnight. The next day cells were treated with the indicated agents. After 72 h, supernatant from treated cells was transferred to a 14 ml tube and combined with adherent cells harvested with 0.25% Trypsin EDTA. For cell cycle analysis cells were washed twice with PBS and 2 × 106 cells/sample were fixed in 1.8 ml cold (-20°C) 70% ethanol followed by 1 h incubation on ice and 24 h incubation in -20°C. Cells were then pelleted and stained in PBS buffer containing 50 μg/ml propidium iodide (PI, Molecular Probes, Invitrogen) with 1 mg/ml RNase A (Sigma-Aldrich) and 0.1% Triton X-100 (Bio-Rad, Richmond, CA) for 15 min at 37°C followed by 1 h incubation on ice. For apoptosis analysis cells were washed twice with Hank's media without phenol red and pellets were resuspended in Annexin-V buffer containing anti-Annexin-V-FITC antibody (Caltag Laboratories, Burlingame, CA). Samples were then incubated on ice for 30 min and counterstained with PI at a final concentration of 1 μg/ml. Flow cytometric analysis was performed with FACSCalibur flow cytometer and acquired data were analyzed with the Cellquest software (Becton-Dickinson, San Jose, CA).
Cells were plated in T25 flasks or 6 cm culture dishes and after overnight adhesion treated with the indicated drugs. After 72 h cells were harvested in ice-cold PBS. Cell pellets were lysed in lysis buffer containing 50 mM Tris pH 7.4, 150 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1 mM EDTA, 0.1% SDS, and Mini Protease Inhibitor Cocktail tables (Roche Diagnostics, Mannheim, Germany). Tumors were homogenized in lysis buffer followed by sonication. After centrifugation (30 min at 13000 rpm) the protein concentration in the supernatant was quantified using the Pierce Micro BCA™ Assay Kit. 30 - 50 μg of total protein per sample was separated on precast 4-12% Bis-Tris gels (NuPage, Invitrogen) and transferred to NuPage 0.45 μm nitrocellulose membranes (Invitrogen). Membranes were blocked with 5% skim milk powder in TBS-T (150 mM NaCl, 50 mM Tris, 0.1% Tween-20, pH 7.4) and incubated overnight with primary antibodies in 5% BSA in TBS-T. The next day membranes were washed 3 times with TBS-T and incubated for 1 h with peroxidase-conjugated secondary antibodies (Promega) in TBS-T containing 5% skim milk. Membranes were washed 3 times with TBS-T and signals were detected by enhanced chemiluminescence (SuperSignal® West Pico Chemiluminescent Substrate, Thermo Scientific) on BioMax Light Film (Kodak). All antibodies used for Western blot analysis were from Cell Signaling Technology (Beverly, MA). The following phospho-specific antibodies were used: P-EGFR (Tyr1086 antibody #2220), P-HER2 (Tyr1221/1222 antibody #2243 or Tyr1248 antibody #2247), P-ERK1/2 (Thr202/Tyr204 antibody #9101), P-AKT (Ser473 antibody #4060), P-70S6K (Thr389 antibody #9206), P-ribosomal protein S6 (Ser235/236 antibody #2211). The β-actin antibody (Sigma-Aldrich) was used as a loading control. Films with visualized protein bands were digitized and the optical density (OD) of bands was measured using UN-SCAN-IT graph and gel digitizing software (Silk Scientific, Inc.). After background subtraction the optical density (OD) value for each individual protein band was corrected for β-actin loading and normalized to the vehicle control expressed as l. Western blot analysis was repeated 2 - 3 times to assure consistency of the results.
Immunohistochemistry, image acquisition and image analysis
10 μm cryosections were cut using a Cryostar HM560 (Microm International GmbH), air dried and then fixed in 50% (v/v) acetone/methanol for 10 min at room temperature. Endothelial cells were stained using a monoclonal antibody to PECAM/CD31 (BD Pharmingen) and fluorescent Alexa 647 secondary antibody (Invitrogen). Terminal deoxyribonucleotide transferase-mediated nick-end labeling (TUNEL) staining was used to label apoptotic cells (In situ Cell Death Detection kit, TMR red; Roche). Proliferating cells were stained using a polyclonal antibody to Ki67 (AbCam) followed by a peroxidase conjugated secondary antibody (Sigma-Aldrich) and metal-enhanced 3,3'-diaminobenzidine substrate (Pierce). Cell nuclei were labeled with Hoechst 33342 (8 ng/mL; Molecular Probes, Invitrogen) for 30 min at 37°C. At each stage of staining, whole tumor sections were imaged using a robotic fluorescence microscope, as previously described [40, 41]. Automated tiling of adjacent microscope fields of view was completed to generate images of an entire tumor section at a resolution of 0.75 μm per pixel. All parameters stained on the same section were imaged separately using a monochrome camera and composite color images were generated using Adobe Photoshop (CS). Using NIH-Image http://rsb.info.nih.gov/nih-image and user-supplied algorithms, digital images were superimposed, aligned and cropped to tumor tissue boundaries with staining artifacts removed. Confluent necrosis was subsequently cropped from images and the degree of necrotic tissue calculated as the proportion of necrotic pixels relative to all pixels. ImageJ software applications http://rsb.info.nih.gov/ij/ and user-supplied algorithms were used to quantify the degree of staining above the thresholds determined to be > 10 standard deviations from background for CD31, TUNEL and Ki67, and data are reported as percent positive pixels of non-necrotic, viable tumor tissue. As a measure of tumor vascularization, the median distance of viable tissue to the nearest CD31-positive object (blood vessel) is reported (μm), such that a larger distance reflects a lower vascular density. Note that for CD31 analysis one JIMT-1 tumor was removed from each of the gefitinib and RAD001-gefitinib combination groups due to the presence of disproportionate necrosis; where only a narrow, avascular rim could be detected as viable tissue. To observe the location of proliferating cells in relation to blood vessels, Ki67 positive pixels were sorted based on their distance from CD31-positive vessels in 1.5 μm increments, and data are expressed as % positive Ki67 pixels relative to distance from vasculature (μm).
One-way ANOVA was used to assess differences among the treatment groups with an unpaired t-test (GraphPad Prism version 5.00). The obtained p values were adjusted for multiple comparisons using the Benjamini-Hochberg procedure (R version 2.11.1). Differences were considered significant at p ≤ 0.05.
Gefitinib and RAD001 in combination synergistically decrease tumor cell viability in vitro
Guided by the IC50 values determined for the single drugs, the gefitinib and RAD001 combinations were evaluated at the 50:1, 200:1 and 1000:1 (Gef:RAD) fixed molar ratios. The effects of the gefitinib and RAD001 combination on cell growth assessed at 200:1 molar ratio (Gef:RAD) are presented in Figure 1B as an example of the data showing that the combination exerted a significantly greater (p < 0.05) growth inhibition in all three cell lines when compared to the single drugs at the dose points indicated by asterisks. These points include therapeutically relevant gefitinib and RAD001 concentrations that can be achieved in human blood and which were previously reported as ~1 μM for gefitinib  and ~ 5-15 nM for rapamycin and derivatives . The dose response data were then evaluated with the CompuSyn™ program which applies the median effect methodology developed by Chou and Talalay  to assess drug-drug interactions. This algorithm estimates a combination index (CI) for each data point based on the results expected from each of the single agents. If the experimental effects of the combination are greater than expected, then the CI value will be less than 1. If the experimental effects are less than expected the CI value will be greater than 1. CI values of 0.8 or less are indicative of robust synergistic interactions. Figure 1C shows that after 144 h treatment with the gefitinib and RAD001 combination, a CI < 0.8 was reported over a wide range of Fa regardless of the drug:drug ratio used, reflecting synergistic interactions between gefitinib and RAD001. Importantly, these data underline the fact that the gefitinib and RAD001 combination is equally effective in HER2 overexpressing cells irrespective of their intrinsic resistance to gefitinib or TZ.
Cytotoxic and cytostatic effects of gefitinib, RAD001 and the combination in vitro
To further investigate the mechanisms of action of the gefitinib and RAD001 combination we performed flow cytometric analysis of apoptosis and cell cycle. Cells were treated for 72 h with 1 μM gefitinib, 5 nM RAD001 or the combination of both drugs at 200:1 molar ratio (Gef:RAD). There was no significant increase (p > 0.05) found in Annexin positive PI negative apoptotic cells in SKBR3 or MCF7-HER2 cultures treated with the combination compared to treatment with the single drugs at corresponding concentrations (Figure 2B). In JIMT-1 cells treated with the combination there was a 3-fold increase in apoptosis relative to gefitinib or RAD001 treatment alone (p < 0.05); however, the absolute percentage of apoptotic cells in the combination treated cultures was only 4% compared to 1% in the single drug treated cultures.
Cells treated in an identical manner as described above were then analyzed for changes in cell cycle. The data summarized in Figure 2C show that the gefitinib and RAD001 combination significantly increased (p < 0.05) the proportion of cells in G1/G0 and reduced the S phase fraction in SKBR3 and JIMT-1 cells, when compared to either of the drugs alone. No significant changes in cell cycle (p > 0.05) were observed in MCF7-HER2 cells (Figure 2C).
Gefitinib and RAD001 in combination decrease activity of the mTOR pathway in vitro
Gefitinib and RAD001 in combination impede growth of established TZ sensitive and TZ resistant tumors
It should be noted that none of the treatment regimens caused any considerable body weight loss in animals (p > 0.05). Detailed animal health monitoring data (not reported here) suggested that gefitinib and RAD001 were well tolerated at the doses used, whether the drugs were used alone or in combination. It is important to note that we also tested sensitivity of JIMT-1 tumors to TZ in Rag2M mice. The results of this study presented in "Additional file 1" show that treatment with TZ (up to 20 mg/kg given twice weekly (i.p.) over the course of 27 days) did not cause inhibition of tumor volume (p > 0.05), thus, confirming the resistance of JIMT-1 cells to TZ, as previously determined by others .
Effects of gefitinib, RAD001 and the combination on tumor tissue characteristics
Evidence presented in Figure 5B suggests that tumor growth inhibition by the gefitinib and RAD001 combination is not associated with an increase in either necrosis or apoptosis. To find out if the growth inhibition is a result of altered proliferation rates of tumor cells, we analyzed the expression of Ki67 in tumor sections. Figure 5C (left graph) shows that in JIMT-1 tumors the combination caused a significant reduction in Ki67 expressing tissue (p < 0.05; 2.5-fold) compared to vehicle treated controls. This is in contrast to the single drugs which did not produced a significant reduction in Ki67 relative to the control group (p > 0.05). In MCF7-HER2 tumors the expression of Ki67 was significantly lower (p < 0.05) in the combination treated group compared to vehicle (1.5-fold decrease) and also to the single drug treated groups (1.9-fold and 1.5-fold decrease compared to gefitinib and RAD001 group, respectively) (Figure 5C, right graph).
When these differences were further investigated by mapping the micro-regional distribution of Ki67 positive pixels relative to CD31-stained vessels, more specific detail was obtained. In JIMT-1 tumors, decreased Ki67 staining was observed in all treatment groups relative to controls and at all distances from vasculature, with the combination showing the greatest effect at tumor tissue in close proximity to vessels (Figure 5D, left graph). A different pattern was observed in MCF7-HER2 tumors, where RAD001 showed no major change relative to controls but gefitinib alone caused a remarkable increase in proliferation both at near proximity and > 100 μM from the vessels. In MCF7-HER2 tumors the combination treatment was the only regimen to affect a drastic decrease in proliferation in tissue proximal to vasculature which, similarly to gefitinib treatment alone, did not decrease further with growing distance from vessels (Figure 5D, right graph). In summary, these data provide evidence that gefitinib and RAD001 when used in combination in vivo do not increase cytotoxicity but interact to increase cytostasis, with greater effects in tumor tissue proximal to vasculature.
Gefitinib and RAD001 in combination decrease levels of P-EGFR and inhibit the mTOR pathway in vivo
New treatment strategies are needed for patients with advanced HER2-positive breast cancers due to very limited therapeutic options available for fighting this disease. Encouraged by previously published reports demonstrating synergistic interactions between EGFR and mTOR inhibitors in various cancers [34–36] we investigated the activity of the EGFR targeted drug gefitinib used in combination with a rapamycin analog, RAD001, in HER2 overexpressing and EGFR co-expressing breast cancer models with TZ sensitive or resistant phenotypes [12, 38, 42, 43]. The rationale to study this combination in HER2 positive breast cancers has also been strengthened by a recent investigation of Miller et al. who demonstrated that inhibition of PI3K and mTOR are required for the growth inhibitory effects of HER2 antagonists in HER2 overexpressing breast cancer and that inhibition of mTOR is an effective therapeutic strategy in TZ resistant breast cancer models .
Our data showed that while SKBR3 cells were sensitive to gefitinib, JIMT-1 and MCF7-HER2 cells were gefitinib resistant; however, RAD001 was capable of sensitizing these cells to gefitinib. It is interesting to note that both JIMT-1 and MCF7-HER2 cell lines harbor PIK3CA mutations which have been associated with acquired resistance to EGFR kinase inhibitors but can also predict sensitivity towards mTOR inhibition [43, 49, 50]. Together with our data, this may suggest that RAD001 is able to reverse gefitinib resistance in PIK3CA mutant tumors. Our data indicate that in vitro gefitinib and RAD001 interact in a synergistic fashion, as shown by a mathematical model developed by Chou and Talaly  and this synergy did not appear to be drug ratio dependent. The in vivo efficacy of gefitinib and RAD001 was also greatly improved when these drugs were used in combination. Further validation of our results in other models of HER2 overexpressing and TZ resistant breast cancers such as MDA-MB-453, MDA-MB-361 or UACC893 would be crucial in order to determine if this combination is broadly effective in TZ resistant cancers. However, our results obtained using the JIMT-1 model do give an indication that the gefitinib and RAD001 combination was able to effectively target the cellular machinery that is indispensable for cancer cell growth despite the existence of multiple mechanisms contributing to the extreme TZ resistance of this cell line [38, 42]. It should be noted that while the combination treatment did not result in regression of established tumors, this could be a consequence of our experimental design. We opted to use doses of gefitinib and RAD001 that on their own did not produce statistically significant (p > 0.05) reduction in tumor volume relative to vehicle treated controls, so that inhibition of tumor growth by the combination would be evident. Consequently, gefitinib given at 100 mg/kg resulted in a more potent reduction in MCF7-HER2 tumor volume than anticipated on its own, thus making the effect of the combination very modest.
The data obtained based on analysis of multiple endpoints after 72 h treatment suggest a contribution of cytostasis in the presence (in SKBR3 and JIMT-1 cells) or absence (in MCF7-HER2 cells) of cytotoxicity to the synergy between gefitinib and RAD001 in vitro. Treatment with the combination induced apoptosis only in JIMT-1 cells; however, it should be noted that Annexin V is a marker for the early apoptotic event so apoptosis may not be detected in SKBR3 and MCF7-HER2 cells after 72 h. Thus, a contribution of apoptosis to cytotoxicity at earlier time points is possible. Our findings are consistent with other reports demonstrating that gefitinib and RAD001 are cytostatic in nature [12, 14, 25, 45] and that the degree of cytotoxicity triggered by these drugs is a cell type dependent phenomenon [14, 48]. This perhaps reflects PIK3CA or other mutations in genes controlling cell growth, proliferation and survival . While the enhancement of cytostasis seen after 72 h in the combination (1 μM gefitinib with 5 nM RAD001) treated SKBR3 and JIMT-1 cells was confirmed by increased G1/G0 cell cycle arrest and decreased S phase relative to the single drugs, the combination failed to induce significant cell cycle changes in MCF7-HER2 cells despite growth inhibition in the absence of cytotoxicity. It has been reported that the parental MCF7 cell line expresses high levels of activated p70S6K and cyclin D1  which may have contributed to somewhat obscure cell cycle regulation, possibly resulting in longer time required to complete a cell cycle or perhaps a transient cell cycle block that was resolved before 72 h. Increased cytostasis by the gefitinib and RAD001 combination in the absence of increased cytotoxicity was also found in vivo in JIMT-1 and MCF7-HER2 tumor xenografts. This may explain why the combination stabilized tumor growth and did not cause tumor regression. Interestingly, gefitinib increased levels of Ki67-positive cells in MCF7-HER2 tumors. These proliferating cells were present at similar frequency in proximal and longer (> 100 μM) distances from the blood vessels suggesting that tissue perfusion in gefitinib treated tumors was perhaps improved. In support, our previous study found that MCF7-HER2 tumors treated with gefitinib contain a greater proportion of functional Hoechst 33342 perfused vessels and this correlated with significantly increased tumor tissue oxygenation resulting in fewer hypoxic cells present . The study of Lu et al. also showed that positive therapeutic responses of cancer cells to EGFR-targeted therapy with cetuximab and gefitinib are associated with downregulation of hypoxia-inducable-factor-1α (HIF-1 α) . Furthermore, Hardee et al. reported that blockade of HER2 signaling in MCF7-HER2 tumors with TZ improved tumor tissue oxygenation and vascular architecture along with increased microvessel density . Thus, we speculate that gefitinib treatment perhaps resulted in vessel normalization. In turn, improved vessel functionality could be responsible for more efficient delivery of drugs to tumor tissue and increased cytostasis. This may explain why MCF7-HER2 tumors were more sensitive to gefitinib than JIMT-1 tumors, even though we found the opposite to be true in vitro in MCF7-HER2 and JIMT-1 cells.
The most striking and consistent therapeutic effect of the combination noted in vitro and in vivo was greater inhibition of the mTOR pathway reflected by decreased P-p70S6K and P-S6 levels relative to the effects of the single drugs. These changes strongly correlated with better efficacy of the combination treatment. Accordingly, several reports suggested that P-p70S6K can be considered as a biomarker for monitoring treatment outcomes in patients receiving mTOR inhibitors [25, 45, 55, 56]. While the combination did not decrease P-EGFR levels in vitro compared to the single drugs, enhanced inhibition of P-EGFR by the combination in vivo appeared to be a consistent molecular event in JIMT-1 and MCF7-HER2 tumors. This can be attributed to inhibition of P-EGFR by both gefitinib and RAD001. The latter effect was not reported in other studies and the mechanisms involved are unclear at this point. Improved inhibition of P-EGFR by the combination in vivo may certainly play a role in downregulation of the mTOR pathway but how this is achieved without robust inhibition of AKT and ERK1/2 activity remains a question for further research. Interestingly, the in vivo reduction in P-EGFR, P-HER2, P-p70S6K and P-S6 levels was mirrored by decreases in total expression of the corresponding proteins in different treatment groups. Similar correlations were observed in vitro for selected proteins in gefitinib and/or combination treated MCF7-HER2 and JIMT-1 cells. These data suggest that inhibition of translation rates or perhaps changes in post-translational events regulating the expression of EGFR, HER2, p70S6K and S6 proteins may have contributed to decreased signaling in addition to direct effects on protein phosphorylation. In contrast, the expression of ERK1/2 and AKT in vivo was not altered after different treatments indicating that changes in phosphorylation levels actually reflected activation status of these proteins.
It should be noted that despite greatly improved inhibition of the mTOR pathway by the gefitinib and RAD001 combination our data suggest lack of or only moderate inhibitory effects of the combination on P-AKT levels. This result can be explained by a RAD001 mediated negative feedback loop. It has been demonstrated that inhibition of mTORC1 by rapamycin analogs initiates p70S6K-dependent feedback signaling resulting in stimulation of mTORC2 and phosphorylation of AKT on Ser473 [24–30]. Our in vitro data show that after 72 h RAD001 increased P-AKT levels in all three cell lines, but addition of gefitinib to RAD001 was able to counteract this effect. RAD001 also enhanced P-ERK1/2 levels in SKBR3 and MCF7-HER2 cells and in JIMT-1 tumors and these results are in agreement with studies showing activation of ERK1/2 through a PI3K-dependent feedback loop following inhibition of mTORC1 in some human cancers . Again, addition of gefitinib to RAD001 counteracted activation of ERK1/2 in SKBR3 cells and in JIMT-1 tumors. Nonetheless, absence of robust inhibition of AKT and ERK1/2 activity in vivo after treatment with the combination is of concern since it may provide cancer cells with a survival advantage and lead to development of drug resistance and escape from cytostasis which consequently would limit treatment efficacy [3–5, 18, 58, 59]. Likewise, other investigators have shown that targeting HER2 and mTOR using the TZ and RAD001 combination inhibits growth of HER2 overexpressing cancers to a greater extent than single agents, but this treatment did not further reduce P-AKT or P-ERK1/2 levels, when compared to the single drug effects . Thus, combining drugs that inhibit function of EGFR/HER2 with dual PI3K/mTOR and MEK pathway inhibitors in order to abolish compensatory mechanisms may eliminate cancer cell survival and perhaps improve therapeutic effects in HER2-positive breast cancers [59–62].
In summary, we showed that the gefitinib and RAD001 combination is therapeutically effective in HER2 overexpressing breast cancers irrespective of their TZ or gefitinib sensitivity status. The combination increased inhibition of cancer growth in vitro and in vivo. The beneficial therapeutic effects of gefitinib and RAD001 when used in combination appear to be related to ability of drugs to achieve efficient inhibition of mTOR (in vitro and in vivo) and EGFR (in vivo) signaling while at the same time eliminating negative feedback effects. Because the gefitinib and RAD001 combination shows a favorable safety profile in vivo and both drugs are approved for human use, this combination could be rapidly translated into opportunities in the clinic.
The authors thank the Canadian Breast Cancer Research Alliance, the Canadian Breast Cancer Foundation, the National Cancer Institute of Canada and Canadian Institutes of Health Research for supporting this project. We also greatly appreciate the help of Dr. Steven McKinney with the statistical analysis, Young Yoo Yang and Dita Strutt with animal work and Hong Yan with tissue culture work.
- Wiseman SM, Makretsov N, Nielsen TO, Gilks B, Yorida E, Cheang M, Turbin D, Gelmon K, Huntsman DG: Coexpression of the type 1 growth factor receptor family members HER-1, HER-2, and HER-3 has a synergistic negative prognostic effect on breast carcinoma survival. Cancer. 2005, 103 (9): 1770-1777. 10.1002/cncr.20970.View ArticlePubMedGoogle Scholar
- Slamon DJ, Clark GM, Wong SG, Levin WJ, Ullrich A, McGuire WL: Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science. 1987, 235 (4785): 177-182. 10.1126/science.3798106.View ArticlePubMedGoogle Scholar
- Knuefermann C, Lu Y, Liu B, Jin W, Liang K, Wu L, Schmidt M, Mills GB, Mendelsohn J, Fan Z: HER2/PI-3K/Akt activation leads to a multidrug resistance in human breast adenocarcinoma cells. Oncogene. 2003, 22 (21): 3205-3212. 10.1038/sj.onc.1206394.View ArticlePubMedGoogle Scholar
- Liang K, Lu Y, Jin W, Ang KK, Milas L, Fan Z: Sensitization of breast cancer cells to radiation by trastuzumab. Mol Cancer Ther. 2003, 2 (11): 1113-1120.PubMedGoogle Scholar
- Hynes NE, Lane HA: ERBB receptors and cancer: the complexity of targeted inhibitors. Nat Rev Cancer. 2005, 5 (5): 341-354. 10.1038/nrc1609.View ArticlePubMedGoogle Scholar
- Vogel CL, Cobleigh MA, Tripathy D, Gutheil JC, Harris LN, Fehrenbacher L, Slamon DJ, Murphy M, Novotny WF, Burchmore M, Shak S, Stewart SJ, Press M: Efficacy and safety of trastuzumab as a single agent in first-line treatment of HER2-overexpressing metastatic breast cancer. J Clin Oncol. 2002, 20 (3): 719-726. 10.1200/JCO.20.3.719.View ArticlePubMedGoogle Scholar
- Cobleigh MA, Vogel CL, Tripathy D, Robert NJ, Scholl S, Fehrenbacher L, Wolter JM, Paton V, Shak S, Lieberman G, Slamon DJ: Multinational study of the efficacy and safety of humanized anti-HER2 monoclonal antibody in women who have HER2-overexpressing metastatic breast cancer that has progressed after chemotherapy for metastatic disease. J Clin Oncol. 1999, 17 (9): 2639-2648.PubMedGoogle Scholar
- Nahta R, Esteva FJ: Trastuzumab: triumphs and tribulations. Oncogene. 2007, 26 (25): 3637-3643. 10.1038/sj.onc.1210379.View ArticlePubMedGoogle Scholar
- Montemurro F, Donadio M, Clavarezza M, Redana S, Jacomuzzi ME, Valabrega G, Danese S, Vietti-Ramus G, Durando A, Venturini M, Aglietta M: Outcome of patients with HER2-positive advanced breast cancer progressing during trastuzumab-based therapy. Oncologist. 2006, 11 (4): 318-324. 10.1634/theoncologist.11-4-318.View ArticlePubMedGoogle Scholar
- Jahanzeb M: Adjuvant trastuzumab therapy for HER2-positive breast cancer. Clin Breast Cancer. 2008, 8 (4): 324-333. 10.3816/CBC.2008.n.037.View ArticlePubMedGoogle Scholar
- DiGiovanna MP, Stern DF, Edgerton SM, Whalen SG, Moore D, Thor AD: Relationship of epidermal growth factor receptor expression to ErbB-2 signaling activity and prognosis in breast cancer patients. J Clin Oncol. 2005, 23 (6): 1152-1160. 10.1200/JCO.2005.09.055.View ArticlePubMedGoogle Scholar
- Warburton C, Dragowska WH, Gelmon K, Chia S, Yan H, Masin D, Denyssevych T, Wallis AE, Bally MB: Treatment of HER-2/neu overexpressing breast cancer xenograft models with trastuzumab (Herceptin) and gefitinib (ZD1839): drug combination effects on tumor growth, HER-2/neu and epidermal growth factor receptor expression, and viable hypoxic cell fraction. Clin Cancer Res. 2004, 10 (7): 2512-2524. 10.1158/1078-0432.CCR-03-0244.View ArticlePubMedGoogle Scholar
- Moasser MM, Basso A, Averbuch SD, Rosen N: The tyrosine kinase inhibitor ZD1839 ("Iressa") inhibits HER2-driven signaling and suppresses the growth of HER2-overexpressing tumor cells. Cancer Res. 2001, 61 (19): 7184-7188.PubMedGoogle Scholar
- Moulder SL, Yakes FM, Muthuswamy SK, Bianco R, Simpson JF, Arteaga CL: Epidermal growth factor receptor (HER1) tyrosine kinase inhibitor ZD1839 (Iressa) inhibits HER2/neu (erbB2)-overexpressing breast cancer cells in vitro and in vivo. Cancer Res. 2001, 61 (24): 8887-8895.PubMedGoogle Scholar
- Emlet DR, Schwartz R, Brown KA, Pollice AA, Smith CA, Shackney SE: HER2 expression as a potential marker for response to therapy targeted to the EGFR. Br J Cancer. 2006, 94 (8): 1144-1153. 10.1038/sj.bjc.6603078.View ArticlePubMedPubMed CentralGoogle Scholar
- Ritter CA, Perez-Torres M, Rinehart C, Guix M, Dugger T, Engelman JA, Arteaga CL: Human breast cancer cells selected for resistance to trastuzumab in vivo overexpress epidermal growth factor receptor and ErbB ligands and remain dependent on the ErbB receptor network. Clin Cancer Res. 2007, 13 (16): 4909-4919. 10.1158/1078-0432.CCR-07-0701.View ArticlePubMedGoogle Scholar
- Chen X, Yeung TK, Wang Z: Enhanced drug resistance in cells coexpressing ErbB2 with EGF receptor or ErbB3. Biochem Biophys Res Commun. 2000, 277 (3): 757-763. 10.1006/bbrc.2000.3731.View ArticlePubMedGoogle Scholar
- Dua R, Zhang J, Nhonthachit P, Penuel E, Petropoulos C, Parry G: EGFR over-expression and activation in high HER2, ER negative breast cancer cell line induces trastuzumab resistance. Breast Cancer Res Treat. 2010, 122 (3): 685-697. 10.1007/s10549-009-0592-x.View ArticlePubMedGoogle Scholar
- Arteaga CL, O'Neill A, Moulder SL, Pins M, Sparano JA, Sledge GW, Davidson NE: A phase I-II study of combined blockade of the ErbB receptor network with trastuzumab and gefitinib in patients with HER2 (ErbB2)-overexpressing metastatic breast cancer. Clin Cancer Res. 2008, 14 (19): 6277-6283. 10.1158/1078-0432.CCR-08-0482.View ArticlePubMedPubMed CentralGoogle Scholar
- D'Alessio A, De Luca A, Maiello MR, Lamura L, Rachiglio AM, Napolitano M, Gallo M, Normanno N: Effects of the combined blockade of EGFR and ErbB-2 on signal transduction and regulation of cell cycle regulatory proteins in breast cancer cells. Breast Cancer Res Treat. 2010, 123 (2): 387-396. 10.1007/s10549-009-0649-x.View ArticlePubMedGoogle Scholar
- Ciardiello F, Caputo R, Bianco R, Damiano V, Fontanini G, Cuccato S, De Placido S, Bianco AR, Tortora G: Inhibition of growth factor production and angiogenesis in human cancer cells by ZD1839 (Iressa), a selective epidermal growth factor receptor tyrosine kinase inhibitor. Clin Cancer Res. 2001, 7 (5): 1459-1465.PubMedGoogle Scholar
- Zhou X, Tan M, Stone Hawthorne V, Klos KS, Lan KH, Yang Y, Yang W, Smith TL, Shi D, Yu D: Activation of the Akt/mammalian target of rapamycin/4E-BP1 pathway by ErbB2 overexpression predicts tumor progression in breast cancers. Clin Cancer Res. 2004, 10 (20): 6779-6788. 10.1158/1078-0432.CCR-04-0112.View ArticlePubMedGoogle Scholar
- Klos KS, Wyszomierski SL, Sun M, Tan M, Zhou X, Li P, Yang W, Yin G, Hittelman WN, Yu D: ErbB2 increases vascular endothelial growth factor protein synthesis via activation of mammalian target of rapamycin/p70S6K leading to increased angiogenesis and spontaneous metastasis of human breast cancer cells. Cancer Res. 2006, 66 (4): 2028-2037. 10.1158/0008-5472.CAN-04-4559.View ArticlePubMedGoogle Scholar
- Yang Q, Guan KL: Expanding mTOR signaling. Cell Res. 2007, 17 (8): 666-681. 10.1038/cr.2007.64.View ArticlePubMedGoogle Scholar
- Lane HA, Breuleux M: Optimal targeting of the mTORC1 kinase in human cancer. Curr Opin Cell Biol. 2009, 21 (2): 219-229. 10.1016/j.ceb.2009.01.016.View ArticlePubMedGoogle Scholar
- Sarbassov DD, Guertin DA, Ali SM, Sabatini DM: Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005, 307 (5712): 1098-1101. 10.1126/science.1106148.View ArticlePubMedGoogle Scholar
- Harrington LS, Findlay GM, Gray A, Tolkacheva T, Wigfield S, Rebholz H, Barnett J, Leslie NR, Cheng S, Shepherd PR, Gout I, Downes CP, Lamb RF: The TSC1-2 tumor suppressor controls insulin-PI3K signaling via regulation of IRS proteins. J Cell Biol. 2004, 166 (2): 213-223. 10.1083/jcb.200403069.View ArticlePubMedPubMed CentralGoogle Scholar
- Dibble CC, Asara JM, Manning BD: Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1. Mol Cell Biol. 2009, 29 (21): 5657-5670. 10.1128/MCB.00735-09.View ArticlePubMedPubMed CentralGoogle Scholar
- O'Reilly KE, Rojo F, She QB, Solit D, Mills GB, Smith D, Lane H, Hofmann F, Hicklin DJ, Ludwig DL, Baselga J, Rosen N: mTOR inhibition induces upstream receptor tyrosine kinase signaling and activates Akt. Cancer Res. 2006, 66 (3): 1500-1508. 10.1158/0008-5472.CAN-05-2925.View ArticlePubMedPubMed CentralGoogle Scholar
- Abraham RT, Gibbons JJ: The mammalian target of rapamycin signaling pathway: twists and turns in the road to cancer therapy. Clin Cancer Res. 2007, 13 (11): 3109-3114. 10.1158/1078-0432.CCR-06-2798.View ArticlePubMedGoogle Scholar
- Ellard SL, Clemons M, Gelmon KA, Norris B, Kennecke H, Chia S, Pritchard K, Eisen A, Vandenberg T, Taylor M, Sauerbrei E, Mishaeli M, Huntsman D, Walsh W, Olivo M, McIntosh L, Seymour L: Randomized phase II study comparing two schedules of everolimus in patients with recurrent/metastatic breast cancer: NCIC Clinical Trials Group IND.163. J Clin Oncol. 2009, 27 (27): 4536-4541. 10.1200/JCO.2008.21.3033.View ArticlePubMedGoogle Scholar
- Chan S, Scheulen ME, Johnston S, Mross K, Cardoso F, Dittrich C, Eiermann W, Hess D, Morant R, Semiglazov V, Borner M, Salzberg M, Ostapenko V, Illiger HJ, Behringer D, Bardy-Bouxin N, Boni J, Kong S, Cincotta M, Moore L: Phase II study of temsirolimus (CCI-779), a novel inhibitor of mTOR, in heavily pretreated patients with locally advanced or metastatic breast cancer. J Clin Oncol. 2005, 23 (23): 5314-5322. 10.1200/JCO.2005.66.130.View ArticlePubMedGoogle Scholar
- Morrow PK, Wulf GM, Ensor J, Booser DJ, Moore JA, Flores PR, Xiong Y, Zhang S, Krop IE, Winer EP, Kindelberger DW, Coviello J, Sahin AA, Nunez R, Hortobagyi GN, Yu D, Esteva FJ: Phase I/II Study of Trastuzumab in Combination With Everolimus (RAD001) in Patients With HER2-Overexpressing Metastatic Breast Cancer Who Progressed on Trastuzumab-Based Therapy. J Clin Oncol. 2011, 29 (23): 3126-32. 10.1200/JCO.2010.32.2321.View ArticlePubMedPubMed CentralGoogle Scholar
- Gemmill RM, Zhou M, Costa L, Korch C, Bukowski RM, Drabkin HA: Synergistic growth inhibition by Iressa and Rapamycin is modulated by VHL mutations in renal cell carcinoma. Br J Cancer. 2005, 92 (12): 2266-2277. 10.1038/sj.bjc.6602646.View ArticlePubMedPubMed CentralGoogle Scholar
- Buck E, Eyzaguirre A, Brown E, Petti F, McCormack S, Haley JD, Iwata KK, Gibson NW, Griffin G: Rapamycin synergizes with the epidermal growth factor receptor inhibitor erlotinib in non-small-cell lung, pancreatic, colon, and breast tumors. Mol Cancer Ther. 2006, 5 (11): 2676-2684. 10.1158/1535-7163.MCT-06-0166.View ArticlePubMedGoogle Scholar
- Bianco R, Garofalo S, Rosa R, Damiano V, Gelardi T, Daniele G, Marciano R, Ciardiello F, Tortora G: Inhibition of mTOR pathway by everolimus cooperates with EGFR inhibitors in human tumours sensitive and resistant to anti-EGFR drugs. Br J Cancer. 2008, 98 (5): 923-930. 10.1038/sj.bjc.6604269.View ArticlePubMedPubMed CentralGoogle Scholar
- Benz CC, Scott GK, Sarup JC, Johnson RM, Tripathy D, Coronado E, Shepard HM, Osborne CK: Estrogen-dependent, tamoxifen-resistant tumorigenic growth of MCF-7 cells transfected with HER2/neu. Breast Cancer Res Treat. 1992, 24 (2): 85-95. 10.1007/BF01961241.View ArticlePubMedGoogle Scholar
- Tanner M, Kapanen AI, Junttila T, Raheem O, Grenman S, Elo J, Elenius K, Isola J: Characterization of a novel cell line established from a patient with Herceptin-resistant breast cancer. Mol Cancer Ther. 2004, 3 (12): 1585-1592.PubMedGoogle Scholar
- Chou TC, Talalay P: Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Regul. 1984, 22: 27-55.View ArticlePubMedGoogle Scholar
- Kyle AH, Huxham LA, Baker JH, Burston HE, Minchinton AI: Tumor distribution of bromodeoxyuridine-labeled cells is strongly dose dependent. Cancer Res. 2003, 63 (18): 5707-5711.PubMedGoogle Scholar
- Baker JH, Lindquist KE, Huxham LA, Kyle AH, Sy JT, Minchinton AI: Direct visualization of heterogeneous extravascular distribution of trastuzumab in human epidermal growth factor receptor type 2 overexpressing xenografts. Clin Cancer Res. 2008, 14 (7): 2171-2179. 10.1158/1078-0432.CCR-07-4465.View ArticlePubMedGoogle Scholar
- Koninki K, Barok M, Tanner M, Staff S, Pitkanen J, Hemmila P, Ilvesaro J, Isola J: Multiple molecular mechanisms underlying trastuzumab and lapatinib resistance in JIMT-1 breast cancer cells. Cancer Lett. 2010, 294 (2): 211-219. 10.1016/j.canlet.2010.02.002.View ArticlePubMedGoogle Scholar
- Weigelt B, Warne PH, Downward J: PIK3CA mutation, but not PTEN loss of function, determines the sensitivity of breast cancer cells to mTOR inhibitory drugs. Oncogene. 2011, 30: 3222-3233. 10.1038/onc.2011.42.View ArticlePubMedGoogle Scholar
- Cohen MH, Williams GA, Sridhara R, Chen G, McGuinn WD, Morse D, Abraham S, Rahman A, Liang C, Lostritto R, Baird A, Pazdur R: United States Food and Drug Administration Drug Approval summary: Gefitinib (ZD1839; Iressa) tablets. Clin Cancer Res. 2004, 10 (4): 1212-1218. 10.1158/1078-0432.CCR-03-0564.View ArticlePubMedGoogle Scholar
- Dancey JE: Therapeutic targets: MTOR and related pathways. Cancer Biol Ther. 2006, 5 (9): 1065-1073. 10.4161/cbt.5.9.3175.View ArticlePubMedGoogle Scholar
- Manegold PC, Paringer C, Kulka U, Krimmel K, Eichhorn ME, Wilkowski R, Jauch KW, Guba M, Bruns CJ: Antiangiogenic therapy with mammalian target of rapamycin inhibitor RAD001 (Everolimus) increases radiosensitivity in solid cancer. Clin Cancer Res. 2008, 14 (3): 892-900. 10.1158/1078-0432.CCR-07-0955.View ArticlePubMedGoogle Scholar
- Lane HA, Wood JM, McSheehy PM, Allegrini PR, Boulay A, Brueggen J, Littlewood-Evans A, Maira SM, Martiny-Baron G, Schnell CR, Sini P, O'Reilly T: mTOR inhibitor RAD001 (everolimus) has antiangiogenic/vascular properties distinct from a VEGFR tyrosine kinase inhibitor. Clin Cancer Res. 2009, 15 (5): 1612-1622. 10.1158/1078-0432.CCR-08-2057.View ArticlePubMedGoogle Scholar
- Miller TW, Forbes JT, Shah C, Wyatt SK, Manning HC, Olivares MG, Sanchez V, Dugger TC, de Matos Granja N, Narasanna A, Cook RS, Kennedy JP, Lindsley CW, Arteaga CL: Inhibition of mammalian target of rapamycin is required for optimal antitumor effect of HER2 inhibitors against HER2-overexpressing cancer cells. Clin Cancer Res. 2009, 15 (23): 7266-7276. 10.1158/1078-0432.CCR-09-1665.View ArticlePubMedPubMed CentralGoogle Scholar
- Sequist LV, Waltman BA, Dias-Santagata D, Digumarthy S, Turke AB, Fidias P, Bergethon K, Shaw AT, Gettinger S, Cosper AK, Akhavanfard S, Heist RS, Temel J, Christensen JG, Wain JC, Lynch TJ, Vernovsky K, Mark EJ, Lanuti M, Iafrate AJ, Mino-Kenudson M, Engelman JA: Genotypic and histolgical evolution of lung cancers acquiring resistance to EGFR inhibitors. Sci Transl Med. 2011, 3 (75): 75ra26-10.1126/scitranslmed.3002003.View ArticlePubMedPubMed CentralGoogle Scholar
- Janku F, Tsimberidou AM, Garrido-Laguna I, Wang X, Luthra R, Hong DS, Naing A, Falchook GS, Moroney JW, Piha-Paul SA, Wheler JJ, Moulder SL, Fu S, Kurzrock R: PIK3CA mutations in patients with advanced cancers treated with PI3K/AKT/mTOR axis inhibitors. Mol Cancer Ther. 2011, 10 (3): 558-565. 10.1158/1535-7163.MCT-10-0994.View ArticlePubMedPubMed CentralGoogle Scholar
- Noh WC, Mondesire WH, Peng J, Jian W, Zhang H, Dong J, Mills GB, Hung MC, Meric-Bernstam F: Determinants of rapamycin sensitivity in breast cancer cells. Clin Cancer Res. 2004, 10 (3): 1013-1023. 10.1158/1078-0432.CCR-03-0043.View ArticlePubMedGoogle Scholar
- Dragowska WH, Verreault M, Yapp DT, Warburton C, Edwards L, Ramsay EC, Huxham LA, Minchinton AI, Gelmon K, Bally MB: Decreased levels of hypoxic cells in gefitinib treated ER+ HER-2 overexpressing MCF-7 breast cancer tumors are associated with hyperactivation of the mTOR pathway: therapeutic implications for combination therapy with rapamycin. Breast Cancer Res Treat. 2007, 106 (3): 319-331. 10.1007/s10549-007-9502-2.View ArticlePubMedGoogle Scholar
- Lu Y, Liang K, Li X, Fan Z: Responses of cancer cells with wild-type or tyrosine kinase domain-mutated epidermal growth factor receptor (EGFR) to EGFR-targeted therapy are linked to downregulation of hypoxia-inducible factor-1alpha. Mol Cancer. 2007, 6: 63-10.1186/1476-4598-6-63.View ArticlePubMedPubMed CentralGoogle Scholar
- Hardee ME, Eapen RJ, Rabbani ZN, Dreher MR, Marks J, Blackwell KL, Dewhirst MW: Her2/neu signaling blockade improves tumor oxygenation in a multifactorial fashion in Her2/neu+ tumors. Cancer Chemother Pharmacol. 2009, 63 (2): 219-228. 10.1007/s00280-008-0729-3.View ArticlePubMedGoogle Scholar
- Boulay A, Zumstein-Mecker S, Stephan C, Beuvink I, Zilbermann F, Haller R, Tobler S, Heusser C, O'Reilly T, Stolz B, Marti A, Thomas G, Lane HA: Antitumor efficacy of intermittent treatment schedules with the rapamycin derivative RAD001 correlates with prolonged inactivation of ribosomal protein S6 kinase 1 in peripheral blood mononuclear cells. Cancer Res. 2004, 64 (1): 252-261. 10.1158/0008-5472.CAN-3554-2.View ArticlePubMedGoogle Scholar
- Noh WC, Kim YH, Kim MS, Koh JS, Kim HA, Moon NM, Paik NS: Activation of the mTOR signaling pathway in breast cancer and its correlation with the clinicopathologic variables. Breast Cancer Res Treat. 2008, 110 (3): 477-483. 10.1007/s10549-007-9746-x.View ArticlePubMedGoogle Scholar
- Carracedo A, Ma L, Teruya-Feldstein J, Rojo F, Salmena L, Alimonti A, Egia A, Sasaki AT, Thomas G, Kozma SC, Papa A, Nardella C, Cantley LC, Baselga J, Pandolfi PP: Inhibition of mTORC1 leads to MAPK pathway activation through a PI3K-dependent feedback loop in human cancer. J Clin Invest. 2008, 118 (9): 3065-3074.PubMedPubMed CentralGoogle Scholar
- Rixe O, Fojo T: Is cell death a critical end point for anticancer therapies or is cytostasis sufficient?. Clin Cancer Res. 2007, 13 (24): 7280-7287. 10.1158/1078-0432.CCR-07-2141.View ArticlePubMedGoogle Scholar
- Garber K: Targeting mTOR: something old, something new. J Natl Cancer Inst. 2009, 101 (5): 288-290. 10.1093/jnci/djp034.View ArticlePubMedGoogle Scholar
- Serra V, Scaltriti M, Prudkin L, Eichhorn PJ, Ibrahim YH, Chandarlapaty S, Markman B, Rodriguez O, Guzman M, Rodriguez S, Gili M, Russillo M, Parra JL, Singh S, Arribas J, Rosen N, Baselga J: PI3K inhibition results in enhanced HER signaling and acquired ERK dependency in HER2-overexpressing breast cancer. Oncogene. 2011, 30 (22): 2547-2557. 10.1038/onc.2010.626.View ArticlePubMedPubMed CentralGoogle Scholar
- Chakrabarty A, Sanchez V, Kuba MG, Rinehart C, Arteaga CL: Breast Cancer Special Feature: Feedback upregulation of HER3 (ErbB3) expression and activity attenuates antitumor effect of PI3K inhibitors. Proc Natl Acad Sci USA. 2011Google Scholar
- Chandarlapaty S, Sawai A, Scaltriti M, Rodrik-Outmezguine V, Grbovic-Huezo O, Serra V, Majumder PK, Baselga J, Rosen N: AKT inhibition relieves feedback suppression of receptor tyrosine kinase expression and activity. Cancer Cell. 2011, 19 (1): 58-71. 10.1016/j.ccr.2010.10.031.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/11/420/prepub
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