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Figure 6 | BMC Cancer

Figure 6

From: The combination of gefitinib and RAD001 inhibits growth of HER2 overexpressing breast cancer cells and tumors irrespective of trastuzumab sensitivity

Figure 6

In vivo molecular responses to gefitinib, RAD001 and the gefitinib and RAD001 combination. Western blot analysis of phospho- and corresponding total protein levels in tissue lysates derived from JIMT-1 and MCF7-HER2 tumors harvested from Rag2M mice at the end of treatment. Animals bearing JIMT-1 tumors were treated with 100 mg/kg gefitinib (Gef), 1.25 mg/kg RAD001 (RAD) and the combination of both drugs and animals bearing MCF7-HER2 were treated with 100 mg/kg gefitinib, 1.75 mg/kg RAD001 and the combination of both drugs. Protein concentration in tumor tissue lysates from individual tumors was adjusted to 50 mg/ml and an equal volume of each individual tumor lysate was pooled for loading gels. Results from six pooled tumor lysates per lane are shown. (A) Representative images of protein bands. (B) The phosphoprotein levels shown in (A) relative to β-actin were determined by densitometry and normalized to the vehicle control expressed as 1 and denoted by a dotted line in each histogram.

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