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Clinical significance of preoperative serum vascular endothelial growth factor, interleukin-6, and C-reactive protein level in colorectal cancer

BMC Cancer volume 10, Article number: 203 (2010) Cite this article

Abstract

Background

Angiogenesis is a multistep process in which many growth factors and cytokines have an essential role. Vascular endothelial growth factor (VEGF) is a potent angiogenic agent that acts as a specific mitogen for vascular endothelial cells through specific cell surface receptors. The interleukin-6 (IL-6) pathway is another mechanism linking angiogenesis to malignancy. C-reactive protein (CRP), a representative marker for inflammation, is known for its association with disease progression in many cancer types. The aim of this study was to determine preoperative serum levels of VEGF, IL-6, and CRP in colorectal carcinoma, and to correlate them with disease status and prognosis.

Methods

A 132 of 143 patients who underwent curative resection for colorectal cancer were enrolled in this study. 11 patients with resection margin positive were excluded. Factors considered in analysis of the relationship between VEGF, IL-6, and CRP and histological findings. Patient prognosis was investigated. Serum levels of VEGF and IL-6 were assessed using Enzyme-Linked Immuno-Sorbent Assay (ELISA), and CRP was measured using immunoturbidimetry.

Results

Median follow-up duration was 18.53 months (range 0.73-43.17 months) and median age of the patients was 62 years (range, 26-83 years). Mean and median levels of VEGF and CRP in colorectal cancer were significantly higher than in the normal control group; 608 vs. 334 pg/mL and 528 (range 122-3242) vs. 312 (range 16-1121) (p < 0.001); 1.05 mg/dL vs. 0.43 mg/dL and 0.22 (range 0.00-18.40) vs. 0.07 (range 0.02-6.94) (p = 0.002), respectively. However mean and median level of IL-6 in patients were not significantly higher than in control; 14.33 pg/mL vs. 5.65 pg/mL and 6.00 (range 1.02-139.17) vs. 5.30 (4.50-13.78) (p = 0.327). Although IL-6 and CRP levels were not correlated with other pathological findings, VEGF level was significantly correlated with tumor size (p = 0.012) and CEA (p = 0.038). When we established the cutoff value for VEGF (825 pg/mL), IL-6 (8.09 pg/mL), and CRP (0.51 mg/dL) by Receiver Operating Characteristic (ROC) curve, we noted that high VEGF levels tended to reduce overall survival (p = 0.053), but not significantly. However, IL-6 and CRP demonstrated no significance with regard to disease free survival (p = 0.531, p = 0.701, respectively) and overall survival (p = 0.563, p = 0.572, respectively). Multivariate analysis showed that VEGF (p = 0.032), CEA (p = 0.012), lymph node metastasis (p = 0.002), and TNM stage (p = 0.025) were independently associated with overall survival.

Conclusions

Preoperative serum VEGF and CRP level increased in colorectal cancer patients. High VEGF level has been proposed as a poor prognostic factor for overall survival in patients with colorectal cancer.

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Background

Colorectal cancer is a common malignant disease, accounting for approximately 15% of all human cancers [1]. In Korea, it is the third most common cancer and the fourth cause of cancer death and its incidence is increasing [2]. Incidence of colorectal cancer has grown rapidly since 1990, and is influenced by eating and lifestyle habits that include increased meat intake and reduced fiber intake, alcohol consumption, and smoking. Current diagnostic techniques and universal colonoscopy have enabled relatively early diagnosis of colorectal cancer, and the cure rate for early stage colorectal cancer is high, at up to 80-90% [3]. Although AJCC TNM classification is useful for staging of colorectal cancer patients and selection for specific treatment, it is not sufficient, as outcome may vary in different patients at the same stage, indicating that conventional staging procedures may not provide accurate prediction of cancer prognosis. Independent of the TNM Stage, the carcinoembryonic antigen (CEA) is used for prediction of prognosis; however, diagnostic sensitivity of CEA is unsatisfactory. Therefore, an appropriate molecular marker is a necessity for adequate treatment of aggressive CRC patients with adjuvant systemic chemotherapy or targeted therapy.

Because new blood vessel formation provides a route for spread of tumor cells to distant organs, angiogenesis as a central process in progression of solid tumors is a well-established aspect of cancer biology [4]. The process may result from an imbalance between positive and negative angiogenic regulators released by both tumor cells and host cells [5]. Tumor angiogenesis and its promoting growth factors have been associated with advanced stage, lymph node involvement and poor survival in a variety of human cancers [6–8]. One of the major pathways associated with this process is that of the vascular endothelial growth factor (VEGF) family of proteins and receptors. Indeed, many tumor cell lines secrete VEGF in vitro, and high VEGF mRNA levels can be detected by in situ hybridization in most human tumors, including lung, breast, and gastrointestinal carcinoma [9]. Both VEFG and its receptor are expressed at high levels in metastatic human colon carcinomas and in tumor associated endothelial cells, and production of these two proteins correlates directly with degree of tumor vascularization [10].

Interleukin-6 (IL-6) is a multipoietic cytokine produced by many cell types. IL-6 acts on a wide range of tissues and cell lines, and induces cell growth and differentiation, production and expression of other cytokines, and acute-phase protein synthesis. IL-6 also promotes growth arrest [11, 12], and promotes angiogenesis through induction of VEGF expression [13, 14].

C-reactive protein (CRP) is an acute reactant protein that is increased under conditions of infection, trauma, tissue necrosis, tumor, and several types of inflammatory disease. It reflects inflammatory status and is a component of the inflammatory response of the immune system [15, 16]. Several studies have reported that in occurrence, progression, metastasis, and recurrence of colorectal cancer, systemic inflammation has an important role, and that CRP may be a useful indicator of inflammatory response. Results from various studies have proven that precancerous lesions are derived from systemic inflammation and local inflammation of the mucous membrane as part of the process of cell degeneration, and that colorectal cancer progresses from adenoma to adenocarcinoma [17, 18].

Therefore, the objective of the current investigation is to assess the prognostic significance of preoperative serum VEGF, IL-6, and CRP levels as indicators of outcome in patients with colorectal cancer.

Methods

Patients

A total of 143 patients who underwent surgery for colorectal cancer at Dong-A University Hospital between December 2005 and December 2008 were enrolled in this study. All patients had histologically confirmed adenocarcinoma of the colon or rectum, and a 132 of the 143 had undergone a potentially curative resection. A 11 of the 143 patients showed microscopic evidence of residual disease; therefore, they were excluded. The control group consisted of 50 persons who underwent health check-ups (25 men and 25 women; median age, 41.5 years; range, 18 to 68 years) absence of neoplastic disease was established in these patients through laboratory testing including serum VEGF, IL-6 and CRP levels. Staging was based on routine postoperative histopathological analysis and clinical assessment by the American Joint Committee on Cancer (AJCC) TNM staging system. Disease status of patients was evaluated by abdominal computed tomography after surgery. CT was checked every 3 months for the first 2 years, and then every 6 months for a total of 5 years. Colonoscopy was performed at 1 year after the resection. Clinical outcomes were followed from the date of surgery to either the date of death or July 2009. A total of 11 colorectal cancer patients died of malignancy and 6 patients were lost during the observation period. The median follow-up period was 18.53 months (range 0.73-43.17). The study was approved by the Institutional Review Board (IRB). All patients provided informed consent, and the hospital review board approved the study.

Assay of serum VEGF, IL-6, and CRP

Venous blood sampling was conducted within a period of 7 days prior to surgery. Blood collected for VEGF and IL-6 serum level assessments was collected in plain tubes, and levels of serum VEGF and IL-6 were measured using commercially available enzyme-linked immunosorbent assays (ELISAs). Blood samples were centrifuged for 10 min at 3,000 r/min at -4°C. Serum was subsequently removed and stored at -80°C for use in biochemical analysis. Blood samples for CRP analysis were collected in serum separation tubes, and serum CRP levels were measured via immunoturbidimetry.

Statistical analysis

Relationships and interactions of the prognostic factors were tested using the Mann-Whitney U-test and the Kruskal Wallis test. Serum levels of VEGF, IL-6, and CRP were expressed as means and median values. VEGF, IL-6, and CRP cut-off values for survival analysis were determined by ROC curve; 825 pg/mL, 11.68 pg/mL, and 0.185 mg/dL, respectively. Duration of recurrence of colorectal cancer and death measured from the date of surgery was referenced against disease free survival and overall survival time. Survival duration was calculated via the Kaplan-Meier method. The log-rank test was employed for comparison of cumulative survival rate and disease free survival in the patient group. For identification of independent factors significantly associated with patient prognosis, we used multivariate analyses employing the Cox proportional hazards model. A p value < 0.05 was considered statistically significant. Variables found to be significant at a level of p < 0.05 were considered eligible for multivatiate regression analysis. The Statistical Package for Social Sciences (SPSS) Version 14.0 (SPSS Inc, Chicago, IL, USA) for Windows was utilized for all statistical analyses.

Results

Patients were classified according to their pathologic characteristics, included tumor size, depth of tumor invasion, status of lymph node (LN) metastasis, lymph node ratio, CEA, American Joint Committee on Cancer tumor-nodes-metastases (AJCC TNM) stage, sex, and age. Patients included 79 men and 53 women, with a median age of 62 years (range, 26-83 years). Characteristic data for the study population are shown in Table 1. Median follow-up duration was 19.25 months (range 0.73-43.17 months).

Table 1 Patient characteristics
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Mean and median levels of VEGF and CRP in the colorectal cancer group were significantly higher than in the normal control group; 620 vs. 334 pg/mL and 541 vs. 312 pg/mL (p < 0.001); 1.12 vs. 0.43 mg/dL and 0.25 vs. 0.07 mg/dL (p = 0.001). However mean and median level of IL-6 in patients were not significantly higher than in the control group; 13.77 vs. 5.65 pg/mL and 5.74 vs. 5.30 pg/mL (p = 0.437), respectively (Table 2).

Table 2 The mean and median levels of VEGF, IL-6 and CRP in colorectal cancer patients and control group
Full size table

Relationships between VEGF, IL-6, and CRP levels, and clinicopathologic variables were tested using the Mann-Whitney U-test and the Kruskal test. Details of these relations are listed in Table 3. VEGF was found to be significantly correlated with tumor size (p = 0.012) and CEA (p = 0.038). But no significantly correlations were observed between VEGF and other variables. IL-6 and CRP levels were not correlated with other pathological findings.

Table 3 Correlation between the IL-6, VEGF, CRP, and clinicopathological parameters
Full size table

The relationship between serum levels of VEGF, IL-6, CRP, and clinicopahologic variables was evaluated using the Kaplan-Meier method. We established the cutoff value for VEGF (825 pg/mL), IL-6 (8.09 pg/mL), and CRP (0.51 mg/dL) by ROC curve. Univariate log-rank analysis showed that CEA (p < 0.001), regional lymph nodes status (p < 0.001), tumor size (p = 0.029), and lymph node ratio (p = 0.001) were reduced overall survival. We noted that high VEGF levels tended to reduce overall survival (p = 0.053, Figure 1), but not significantly. VEGF levels demonstrated no significance with regard to disease free survival (p = 0.236, Figure 2). IL-6 and CRP levels provided no significant evidence with regard to overall patient survival (p = 0.956, p = 0.607 respectively) and disease free survival (p = 0.531, p = 0.701, respectively). Multivariate regression analysis using Cox's proportional hazards model revealed that VEGF (p = 0.032, HR = 4.779), CEA (p = 0.012, HR = 7.981), regional lymph nodes status (p < 0.001), and age (p = 0.005, HR 11.91) were independent prognostic factors for survival of colorectal cancer (Table 4).

Figure 1
figure 1

Overall survival curve according to VEGF level.

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Figure 2
figure 2

Disease free survival curve according to VEGF level.

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Table 4 Multivariate analysis on OS and DFS in 143 colorectal cancer patients
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Discussion

Recent studies have shown the correlation between tissue VEGF expression and tumor aggressiveness in colon carcinoma. VEGF expression was found to be higher in patients who had metastatic tumors compared with patients who had non-metastatic tumors [10], [19, 20]. Takahashi et al [21] showed that VEGF expression level in patients with lymph node negative colon carcinoma was significantly associated with time of recurrence, whereas Cascinu et al [22] confirmed that positive VEGF status was associated with a significant reduction in 5-year DFS rate.

The prognostic impact of serum VEGF levels in cancer patients has been evaluated in a few studies. A large study by Danish Colorectal Cancer Study Group found that high preoperative VEFG concentrations were associated with reduced overall survival with respect to patients with colon carcinoma who had lower VEGF serum values, and suggested a biologically relevant role for serum VEGF concentration in patients with colorectal carcinoma [23–25].

According to findings from recent studies, VEGF has been proposed as a potential biomarker for prediction of colorectal cancer prognosis. De Vita et al [26] determined preoperative and post operative serum VEGF levels by ELISA in patients with colon cancer who underwent surgery. They found that preoperative VEGF was significantly higher in the colon cancer compared with the control group, and preoperative VEGF was significantly lower in patients who underwent curative surgery compared with patients who underwent non-curative surgery. Therefore, they suggested that preoperative serum VEGF levels might be useful for prediction of outcome in patients with colon cancer who underwent surgery. Another study investigated VEGF expression in colorectal cancer specimens by performance of immunohistochemistry on tissue microarrays [27]. In that study, the authors found VEGF expression in all tumors evaluated; they also found greater intensity of VEGF staining, greater lymph node metastasis, higher stage, and poorer disease-specific survival. They concluded that VEGF expression in colorectal cancer appears to be an independent prognostic marker of tumor behavior, and that it may be useful in identification of patients with unfavorable clinical outcome.

In our study, preoperative serum VEGF level by ELISA in the patient group was significantly higher than levels in the control group (p < 0.001) and preoperative serum VEGF levels were significantly correlated with CEA levels (p = 0.038) and tumor size (p = 0.012). When we established the cutoff value for VEGF (825 pg/mL) by ROC curve, we noted that high VEGF levels tended to reduce overall survival, but was not significantly (p = 0.053). No significant correlation was found between VEGF preoperative levels and disease stage, gender, tumor depth, and status of lymph node metastasis.

Currently, ample literature is available with regard to the role of various cytokines in colorectal cancer patients. Mechanisms leading to IL-6 induction and to IL-6 presence in high concentrations in the serum of those patients include CEA-induced IL-6 production by Kupffer cells, malignancy-related chronic stress leading to increased IL-6 blood concentrations, as well as direct IL-6 production and secretion by tumor-associated macrophages or the tumor cells themselves. According to some studies that evaluated clinical significance of IL-6 pre-treatment levels, IL-6 concentrations reflected disease status, and were commonly associated with metastatic disease [28, 29]. Findings from other research did not reveal a significant association between IL-6 and tumor stage, or other clinical findings [30]. However, the authors of that study found significant correlation between large tumor size and hepatic metastasis correlated with elevated IL-6 levels significantly. Furthermore, high IL-6 levels were associated with reduced overall survival. Different from prior studies, was the fact that it was comprised more of non-metastatic stages, which strengthens the clinical relevance of this observation. They suggested that in the absence of advanced metastatic disease, IL-6 levels may be used as an adverse prognostic marker.

CRP is synthesized in hepatocytes and produced by other organs in response to release of IL-6 by monocytes and other immune cells [31]. Increased CRP level has been previously reported in patients with impaired T lymphocyte response; thus this mechanism is considered to have an association with the immune system and poor survival rate [32]. Elevated levels of this marker of infection have been associated with increased risk of cardiovascular disease [33], and have been identified as a poor prognostic factor for several diseases, including a variety of cancers in a number of previous studies [34–37]. Findings from studies in patients with colorectal cancer have indicated that those with elevated serum CRP concentrations had poorer prognoses than those whose CRP level did not increase. In addition, it has been suggested that increased CRP was associated with more frequent local tumor invasion [38], more advanced pathologic stage [39], a higher rate of recurrence [40], and reduced overall survival [30].

Unlike results from earlier studies, we were unable to determine the correlation between IL-6 and CRP and prognostic factors. Although IL-6 and CRP levels showed no correlation with other pathological findings, mean and median levels of CRP in colorectal cancer patients were significantly higher than those in normal individuals (p = 0.001). When we established the cutoff value for IL-6 (11.68 pg/mL), and CRP (0.185 mg/dL) by ROC curve, IL-6 and CRP showed no significant evidence with regard to disease free survival (p = 0.701, p = 0.531, respectively) and overall survival (p = 0.563, p = 0.572, respectively).

This retrospective study had some limitations. First, the patient group was restricted to patients who underwent surgery. Thus this study included more patients who were able to undergo surgery at a lower stage (not included stage IV). And we did not consider patients who underwent surgery or those who had any concomitant disease suspected of raising VEGF, IL-6, or CRP (i.e., chronic inflammatory disorders, diabetes mellitus, ischemic heart disease, etc.). Thus, these were possible causes for misinterpretation of results. The most important limitation of this study is the lack of post operative VEGF, IL-6 and CRP levels. De Vita et al.[26] suggested preoperative VEGF and CEA levels as good prognostic indicators for curative and noncurative surgery, and VEGF levels dropped significantly after surgery, with a further downward trend until the 30th postoperative day. However if we had determined postoperative serum levels of VEGF, IL-6, and CRP, we would have been able to obtain more information with regard to prognosis. For examples, normalization of postoperative serum levels could suggest radical surgery; serum level decrement would show secretion of the marker by the tumor, and patients undergoing potentially curative surgery who does not show serum level normalization of the marker should undergo treatment with chemotherapy regardless of the TNM stage, and increased serum levels during the follow-up period could be prediction of recurrence.

Conclusively, high preoperative VEGF levels were associated with tumor size, higher CEA, and VEGF was independent of overall survival with CEA and lymph node status. Thus we surmise that the prognosis in colorectal cancer patients with high VEGF levels would be generally poor.

Conclusions

As a result of the current study, VEGF is suggested as an important prognostic factor for colorectal cancer. For the future, more prospective studies will be necessary for investigation of predictive biomarkers for colorectal cancer.

References

  1. Giatromanolaki A, Sivridis E, Koukourakis MI: Angiogenesis in colorectal cancer: prognostic and therapeutic implications. Am J Clin Oncol. 2006, 29 (4): 408-417. 10.1097/01.coc.0000221317.56731.4e.

    Article  PubMed  Google Scholar 

  2. Won YJ, Sung J, Jung KW, Kong HJ, Park S, Shin HR, Park EC, Ahn YO, Hwang IK, Lee DH, et al: Nationwide cancer incidence in Korea, 2003-2005. Cancer Res Treat. 2009, 41 (3): 122-131. 10.4143/crt.2009.41.3.122.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Compton CC, Greene FL: The staging of colorectal cancer: 2004 and beyond. CA Cancer J Clin. 2004, 54 (6): 295-308. 10.3322/canjclin.54.6.295.

    Article  PubMed  Google Scholar 

  4. Folkman J: What is the evidence that tumors are angiogenesis dependent?. J Natl Cancer Inst. 1990, 82 (1): 4-6. 10.1093/jnci/82.1.4.

    Article  CAS  PubMed  Google Scholar 

  5. Rak J, Filmus J, Finkenzeller G, Grugel S, Marme D, Kerbel RS: Oncogenes as inducers of tumor angiogenesis. Cancer Metastasis Rev. 1995, 14 (4): 263-277. 10.1007/BF00690598.

    Article  CAS  PubMed  Google Scholar 

  6. Brown LF, Detmar M, Claffey K, Nagy JA, Feng D, Dvorak AM, Dvorak HF: Vascular permeability factor/vascular endothelial growth factor: a multifunctional angiogenic cytokine. EXS. 1997, 79: 233-269.

    CAS  PubMed  Google Scholar 

  7. Dvorak HF, Brown LF, Detmar M, Dvorak AM: Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis. Am J Pathol. 1995, 146 (5): 1029-1039.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Reinmuth N, Parikh AA, Ahmad SA, Liu W, Stoeltzing O, Fan F, Takeda A, Akagi M, Ellis LM: Biology of angiogenesis in tumors of the gastrointestinal tract. Microsc Res Tech. 2003, 60 (2): 199-207. 10.1002/jemt.10258.

    Article  CAS  PubMed  Google Scholar 

  9. Ferrara N: Molecular and biological properties of vascular endothelial growth factor. J Mol Med. 1999, 77 (7): 527-543. 10.1007/s001099900019.

    Article  CAS  PubMed  Google Scholar 

  10. Takahashi Y, Kitadai Y, Bucana CD, Cleary KR, Ellis LM: Expression of vascular endothelial growth factor and its receptor, KDR, correlates with vascularity, metastasis, and proliferation of human colon cancer. Cancer Res. 1995, 55 (18): 3964-3968.

    CAS  PubMed  Google Scholar 

  11. Van Snick J: Interleukin-6: an overview. Annu Rev Immunol. 1990, 8: 253-278. 10.1146/annurev.iy.08.040190.001345.

    Article  CAS  PubMed  Google Scholar 

  12. Sehgal PB: Regulation of IL6 gene expression. Res Immunol. 1992, 143 (7): 724-734. 10.1016/0923-2494(92)80011-9.

    Article  CAS  PubMed  Google Scholar 

  13. Cohen T, Nahari D, Cerem LW, Neufeld G, Levi BZ: Interleukin 6 induces the expression of vascular endothelial growth factor. J Biol Chem. 1996, 271 (2): 736-741. 10.1074/jbc.271.2.736.

    Article  CAS  PubMed  Google Scholar 

  14. Thong-Ngam D, Tangkijvanich P, Lerknimitr R, Mahachai V, Theamboonlers A, Poovorawan Y: Diagnostic role of serum interleukin-18 in gastric cancer patients. World J Gastroenterol. 2006, 12 (28): 4473-4477.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Ballou SP, Lozanski G: Induction of inflammatory cytokine release from cultured human monocytes by C-reactive protein. Cytokine. 1992, 4 (5): 361-368. 10.1016/1043-4666(92)90079-7.

    Article  CAS  PubMed  Google Scholar 

  16. Cermak J, Key NS, Bach RR, Balla J, Jacob HS, Vercellotti GM: C-reactive protein induces human peripheral blood monocytes to synthesize tissue factor. Blood. 1993, 82 (2): 513-520.

    CAS  PubMed  Google Scholar 

  17. Law DJ, Olschwang S, Monpezat JP, Lefrancois D, Jagelman D, Petrelli NJ, Thomas G, Feinberg AP: Concerted nonsyntenic allelic loss in human colorectal carcinoma. Science. 1988, 241 (4868): 961-965. 10.1126/science.2841761.

    Article  CAS  PubMed  Google Scholar 

  18. Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AM, Bos JL: Genetic alterations during colorectal-tumor development. N Engl J Med. 1988, 319 (9): 525-532. 10.1056/NEJM198809013190901.

    Article  CAS  PubMed  Google Scholar 

  19. Tokunaga T, Oshika Y, Abe Y, Ozeki Y, Sadahiro S, Kijima H, Tsuchida T, Yamazaki H, Ueyama Y, Tamaoki N, et al: Vascular endothelial growth factor (VEGF) mRNA isoform expression pattern is correlated with liver metastasis and poor prognosis in colon cancer. Br J Cancer. 1998, 77 (6): 998-1002.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Ishigami SI, Arii S, Furutani M, Niwano M, Harada T, Mizumoto M, Mori A, Onodera H, Imamura M: Predictive value of vascular endothelial growth factor (VEGF) in metastasis and prognosis of human colorectal cancer. Br J Cancer. 1998, 78 (10): 1379-1384.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Takahashi Y, Tucker SL, Kitadai Y, Koura AN, Bucana CD, Cleary KR, Ellis LM: Vessel counts and expression of vascular endothelial growth factor as prognostic factors in node-negative colon cancer. Arch Surg. 1997, 132 (5): 541-546.

    Article  CAS  PubMed  Google Scholar 

  22. Cascinu S, Staccioli MP, Gasparini G, Giordani P, Catalano V, Ghiselli R, Rossi C, Baldelli AM, Graziano F, Saba V, et al: Expression of vascular endothelial growth factor can predict event-free survival in stage II colon cancer. Clin Cancer Res. 2000, 6 (7): 2803-2807.

    CAS  PubMed  Google Scholar 

  23. Werther K, Christensen IJ, Brunner N, Nielsen HJ: Soluble vascular endothelial growth factor levels in patients with primary colorectal carcinoma. The Danish RANX05 Colorectal Cancer Study Group. Eur J Surg Oncol. 2000, 26 (7): 657-662. 10.1053/ejso.2000.0977.

    Article  CAS  PubMed  Google Scholar 

  24. Werther K, Christensen IJ, Nielsen HJ: The association between preoperative concentration of soluble vascular endothelial growth factor, perioperative blood transfusion, and survival in patients with primary colorectal cancer. Eur J Surg. 2001, 167 (4): 287-292. 10.1080/110241501300091480.

    Article  CAS  PubMed  Google Scholar 

  25. Werther K, Christensen IJ, Nielsen HJ: Prognostic impact of matched preoperative plasma and serum VEGF in patients with primary colorectal carcinoma. Br J Cancer. 2002, 86 (3): 417-423. 10.1038/sj.bjc.6600075.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. De Vita F, Orditura M, Lieto E, Infusino S, Morgillo F, Martinelli E, Castellano P, Romano C, Ciardiello F, Catalano G, et al: Elevated perioperative serum vascular endothelial growth factor levels in patients with colon carcinoma. Cancer. 2004, 100 (2): 270-278. 10.1002/cncr.11911.

    Article  PubMed  Google Scholar 

  27. Zafirellis K, Agrogiannis G, Zachaki A, Gravani K, Karameris A, Kombouras C: Prognostic significance of VEGF expression evaluated by quantitative immunohistochemical analysis in colorectal cancer. J Surg Res. 2008, 147 (1): 99-107. 10.1016/j.jss.2007.05.041.

    Article  CAS  PubMed  Google Scholar 

  28. Belluco C, Nitti D, Frantz M, Toppan P, Basso D, Plebani M, Lise M, Jessup JM: Interleukin-6 blood level is associated with circulating carcinoembryonic antigen and prognosis in patients with colorectal cancer. Ann Surg Oncol. 2000, 7 (2): 133-138. 10.1007/s10434-000-0133-7.

    Article  CAS  PubMed  Google Scholar 

  29. Galizia G, Orditura M, Romano C, Lieto E, Castellano P, Pelosio L, Imperatore V, Catalano G, Pignatelli C, De Vita F: Prognostic significance of circulating IL-10 and IL-6 serum levels in colon cancer patients undergoing surgery. Clin Immunol. 2002, 102 (2): 169-178. 10.1006/clim.2001.5163.

    Article  CAS  PubMed  Google Scholar 

  30. Nikiteas NI, Tzanakis N, Gazouli M, Rallis G, Daniilidis K, Theodoropoulos G, Kostakis A, Peros G: Serum IL-6, TNFalpha and CRP levels in Greek colorectal cancer patients: prognostic implications. World J Gastroenterol. 2005, 11 (11): 1639-1643.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Castell JV, Gomez-Lechon MJ, David M, Hirano T, Kishimoto T, Heinrich PC: Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes. FEBS Lett. 1988, 232 (2): 347-350. 10.1016/0014-5793(88)80766-X.

    Article  CAS  PubMed  Google Scholar 

  32. Crumley AB, McMillan DC, McKernan M, Going JJ, Shearer CJ, Stuart RC: An elevated C-reactive protein concentration, prior to surgery, predicts poor cancer-specific survival in patients undergoing resection for gastro-oesophageal cancer. Br J Cancer. 2006, 94 (11): 1568-1571.

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Ridker PM: Clinical application of C-reactive protein for cardiovascular disease detection and prevention. Circulation. 2003, 107 (3): 363-369. 10.1161/01.CIR.0000053730.47739.3C.

    Article  PubMed  Google Scholar 

  34. Trichopoulos D, Psaltopoulou T, Orfanos P, Trichopoulou A, Boffetta P: Plasma C-reactive protein and risk of cancer: a prospective study from Greece. Cancer Epidemiol Biomarkers Prev. 2006, 15 (2): 381-384. 10.1158/1055-9965.EPI-05-0626.

    Article  CAS  PubMed  Google Scholar 

  35. Krzystek-Korpacka M, Matusiewicz M, Diakowska D, Grabowski K, Blachut K, Kustrzeba-Wojcicka I, Terlecki G, Gamian A: Acute-phase response proteins are related to cachexia and accelerated angiogenesis in gastroesophageal cancers. Clin Chem Lab Med. 2008, 46 (3): 359-364. 10.1515/CCLM.2008.089.

    Article  CAS  PubMed  Google Scholar 

  36. Zhang SM, Lin J, Cook NR, Lee IM, Manson JE, Buring JE, Ridker PM: C-reactive protein and risk of breast cancer. J Natl Cancer Inst. 2007, 99 (11): 890-894. 10.1093/jnci/djk202.

    Article  CAS  PubMed  Google Scholar 

  37. Denizot Y, Fixe P, Liozon E, Praloran V: Serum interleukin-8 (IL-8) and IL-6 concentrations in patients with hematologic malignancies. Blood. 1996, 87 (9): 4016-4017.

    CAS  PubMed  Google Scholar 

  38. Nozoe T, Matsumata T, Sugimachi K: Preoperative elevation of serum C-reactive protein is related to impaired immunity in patients with colorectal cancer. Am J Clin Oncol. 2000, 23 (3): 263-266.

    Article  CAS  PubMed  Google Scholar 

  39. Nozoe T, Matsumata T, Kitamura M, Sugimachi K: Significance of preoperative elevation of serum C-reactive protein as an indicator for prognosis in colorectal cancer. Am J Surg. 1998, 176 (4): 335-338. 10.1016/S0002-9610(98)00204-9.

    Article  CAS  PubMed  Google Scholar 

  40. McMillan DC, Wotherspoon HA, Fearon KC, Sturgeon C, Cooke TG, McArdle CS: A prospective study of tumor recurrence and the acute-phase response after apparently curative colorectal cancer surgery. Am J Surg. 1995, 170 (4): 319-322. 10.1016/S0002-9610(99)80296-7.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This paper was supported by the Dong-A University Research Fund.

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    Authors and Affiliations

    1. Department of Internal Medicine, Dong-A university College of Medicine, Busan, South Korea

      Kyung A Kwon, Sung Hyun Kim, Sung Yong Oh, Suee Lee, Hyo-Jin Kim, Hyuk-Chan Kwon & Jong Hoon Lee

    2. Department of Laboratory Medicine, Dong-A university College of Medicine, Busan, South Korea

      Jin-Yeong Han, Kyeong Hee Kim & Ri Young Goh

    3. Department of Surgery, Dong-A university College of Medicine, Busan, South Korea

      Hong Jo Choi & Ki Jae Park

    4. Department of Pathology, Dong-A university College of Medicine, Busan, South Korea

      Mee Sook Roh

    5. Medical Research Center for Cancer Molecular Therapy, Dong-A University College of Medicine, Busan, South Korea

      Mee Sook Roh & Hyuk-Chan Kwon

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    Corresponding author

    Correspondence to Hyuk-Chan Kwon.

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    Competing interests

    The authors declare that they have no competing interests.

    Authors' contributions

    KAK performed the statistical analysis and drafted the manuscript. H-CK collected the data, performed the statistical analysis with interpretation and critically revised the manuscript. SHK, SYO and SL performed the chemotherapy for patients and revised the manuscript. HJC and KJP performed the operations for patients and revised the manuscript. KHK, J-YH, RYG and MSR carried out the immunoassays. JHL and H-JK conceived of the study, and approved the final manuscript. All authors read and approved the final manuscript.

    and Jong Hoon Lee contributed equally to this work.

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    This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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    Kwon, K.A., Kim, S.H., Oh, S.Y. et al. Clinical significance of preoperative serum vascular endothelial growth factor, interleukin-6, and C-reactive protein level in colorectal cancer. BMC Cancer 10, 203 (2010). https://doi.org/10.1186/1471-2407-10-203

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    • DOI: https://doi.org/10.1186/1471-2407-10-203

    Keywords

    • Vascular Endothelial Growth Factor
    • Vascular Endothelial Growth Factor Expression
    • Vascular Endothelial Growth Factor Level
    • Serum Vascular Endothelial Growth Factor
    • Serum Vascular Endothelial Growth Factor Level

    BMC Cancer

    ISSN: 1471-2407

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