Fig. 3

The qRT-PCR and western blotting of cervical cancer cell lines. C/EBPβ ( +): pcDNA3.1-C/EBPβ plasmid transfection; WT: without any transfection; NC: negative control pcDNA3.1 plasmid transfection. The Student’s t-test test was used to compare the two groups of single variable data, single factor analysis of variance comparing multiple groups of data, nonparametric test to compare the variance not neat, count data using chi-square test. Ns: not significant. ^*P < 0.05, ^**P < 0.01. A, B, C, D, E qRT-PCR of the expression of C/EBPβ mRNA in Hela and SiHa cells (expression ratio: C/EBPβ/GAPDH). The experiment was repeated three times. A For measuring C/EBPβ mRNA expression in overexpression cells, pcDNA 3.1-C/EBPβ plasmid was transfected into HeLa and SiHa cells for 48 h. B For measuring C/EBPβ mRNA expression in control cells, pcDNA3.1-Mock plasmid was transfected into HeLa and SiHa cells for 48 h. C Merged (A,B) mRNA expression data in pcDNA 3.1-C/EBPβ and pcDNA3.1-Mock-transfected cells. D For measuring miR-661 expression in overexpression cells, plasmids were transfected into HeLa cells and SiHa cells for 48 h. E For measuring MTA1 mRNA expression in overexpression cells, plasmids were transfected into HeLa and SiHa cells for 48 h. F, G, H, I Western blotting to detect effects of C/EBPβ overexpression on MTA1 expression. The experiment was repeated five times. F, G Plasmid transfection in HeLa cells after 72 h. H, I Plasmid transfection in SiHa cells after 72 h