Fig. 5

Differentially expressed genes regulated by ATBF1were analyzed by microarray and bioinformatics assay. MCF7 cells were transfected with siControl or siATBF1 at the concentration of 150 nM. The total mRNA were extracted for microarray analysis after 48 h. A Two-dimensional principal component analysis (PCA) plot. B Volcano map. Green dots, significantly down-regulated genes; red dots, significantly up-regulated genes; black dots, genes with no significant difference. C Hierarchical cluster of differentially expressed genes. The distances of the gene branches in the left dendrogram represent the similarity of the expression levels. D Top 30 genes regulated by ATBF1 were ranked with P value. Red, up-regulated genes; green, down-regulated genes