Fig. 3

SRPK1 inhibition promoted apoptosis in ENKTL cells. A YT cells were transfected with SRPK1 siRNA or treated with SPHINX31 (12.5 μM) or SRPIN340 (12.5 μM) for 24 h. The cells were analyzed by flow cytometry after Annexin V/7-AAD double staining. The percentage of Annexin V-positive cells is presented as the mean ± SD from 3 independent experiments (*P < 0.05). B Western blotting of whole-cell extracts from YT cells showed increased levels of cleaved PARP and cleaved caspase-3. The Expression levels of cleaved PARP/GAPDH and cleaved caspase-3/GAPDH are presented as the means ± SDs from 3 independent experiments (*P < 0.05). C PBLs from ENKTL patients were treated with SPHINX31 (12.5 μM) or SRPIN340 (12.5 μM) for 24 h, and apoptotic cells were dtected by Annexin V/7-AAD double staining