Fig. 3
From: Adjuvant effect of IRES-based single-stranded RNA on melanoma immunotherapy
Ova protein enhances CrPVIRES-ssRNA-mediated conventional DC 1 activation. A The mice were primed and boosted with Ova protein (50 µg) formulated with CrPVIRES-ssRNA (20 µg) or poly(I:C) (20 µg) (n = 5). Splenocytes and drain inguinal lymph nodes were harvested 1 day after boosting. B Frequency of co-stimulatory CD40, CD80, and CD86 of CD8+ cDC1 cells. C, D XCR1 expression of CD8.+ cDC1 was measured in splenocytes (C) and lymphocytes (D) isolated from drain inguinal lymph nodes. Data were represented as mean ± SD. Statistical significance was indicated by *p < 0.05, **p < 0.01, and ***p < 0.005