Fig. 4

HPV E7 can stabilize the different PDZ targets of E6 and this activity is conserved between the E7 proteins from several high-risk HPVs. A C4-1 wild type cells and the CKII HPV-18 E7 mutated cell lines (C4-1 B8 and C4-1 A15) were grown for 24 h and then whole protein extracts were analysed by Western Blot (Left panel). The endogenous expression of hScrib was then studied using a specific mouse-anti-Scrib antibody. Original blots are presented in Fig. S6. Right panel, densitometry analysis of Western blots for hScrib expression for equivalent α-tubulin levels in each corresponding different condition (mean ± SD, n = 3). Asterisks denote significant difference determined by ANOVA test (*p < 0.05). B HEK293 epithelial cells were transiently transfected with 2.5 µg of pegfp-16 E6, 2.5 µg of wild type or mutant pCMV-Flag-16 E7 and 0.5 µg of pcDNA-HA-Scrib plasmid. Cells were harvested 24 h post-transfection and the expression of fusion proteins was assessed by Western Blot (Left panel) using an anti-Flag (E7), anti-GFP (E6) and anti-HA (hScrib). β-Galactoside levels were used as transfection control. Images with different exposure levels and original blots are presented in Fig. S7. Right panel, densitometry analysis of western blots for hScrib expression for equal β-galactoside levels in each corresponding different condition (mean ± SD, n = 3). Asterisks denote significant difference determined by ANOVA test and a Multiple Comparisons Tukey´s test (**p < 0.05, ***p < 0.005) and “ns” indicates no significant changes