Fig. 6

Involvement of the Gi pathway in LPAR3-mediated survival and migration of NIH 3 T3 cells. A Cell viability was evaluated in parental and LPAR3 KO NIH 3 T3 cells treated with PTX (100 ng/mL) or YM-254890 (10 μM). Viability was expressed as the mean ± SD of quadruplicates. B Phosphorylated MEK, ERK, and AKT were detected through immunoblotting in parental and LPAR3 KO NIH 3 T3 cells treated with PTX for 48 h. The indicated values below each band represent normalized ratio of phosphorylated protein to total protein. C A wound scratch assay was performed with parental NIH 3 T3 and LPAR3 KO cells treated with or without PTX. Representative images of scratch wound closure were photographed right and 12 h after the scratch (left panel). Photographs shown in left panel was analyzed using the TScratch software for determining the size of cell-covered areas (right panel). *P < 0.05; **P < 0.01 compared to control