Fig. 2

Effect of LPAR3 on cell survival and migration of parental and Ras-NIH 3 T3 cells. A Immunofluorescence analysis was performed to detect the endogenous expression of LPAR3 in NIH 3 T3 and Ras-NIH 3 T3 cells. Green and blue indicate LPAR3 expression and Hoechst 33342 nuclear staining, respectively. B Cell viability was evaluated in parental and Ras-NIH 3 T3 cells transiently transfected with LPAR3 for the indicated days. Results were expressed as the mean ± SD of quadruplicate determinations. C Transwell migration assays were performed with parental and Ras-NIH 3 T3 cells transiently transfected with LPAR3. Results were expressed as the mean ± SD of quadruplicate determinations. D Cell viability was determined in parental and Ras-NIH 3 T3 cells transiently transfected with LPAR3 siRNA or a nontargeting control siRNA for 24 h, followed by incubation in 96-well plate for 3 days. Results were expressed as the mean ± SD of quadruplicate determinations. LPAR3 knockdown was verified using qPCR (right inset). E Cell viability was determined in parental and Ras-NIH 3 T3 cells treated with or without (2S)-OMPT or Kil16425 for 72 h. Results were expressed as the mean ± SD of quadruplicate determinations. **P < 0.01 compared to NIH 3 T3 cells