Fig. 1

Relative gene expression of genes with hypermethylated DMRs in parental and their transformed cells. For quantitative analysis of gene expression, the comparative threshold cycle (Ct) Method for relative quantification (2^−ΔΔCt) was used. The expression of the target genes was normalized to GAPDH expression. Values represent the mean ± SD of quadruplicate determinants from one of three representative experiments. **P < 0.01 and *P < 0.05 as determined by the unpaired t-test compared to parental cells