Fig. 3

DDLPS is more fibrotic and heterogeneous with more macrophages compared to rpWAT or WDLPS tissues. A Hematoxylin and eosin (H&E) or trichrome stained DDLPS, WDLPS, and rpWAT. Trichrome stains connective tissue, an indication of fibrosis. B In situ hybridization detection of SIAH2 (yellow) and immunohistochemistry detection of nuclei (DAPI, blue) and macrophages using IBA1 (magenta) in DDLPS, WDLPS, and rpWAT. Section of the image, marked by the white square, was enlarged to aide with SIAH2 visualization. The images shown are representative of N = 3 for rpWAT, N = 4 for WDLPS and N = 5 for DDLPS. C The ratio of SIAH2 mRNA to nuclei in the images shown in Figs. 1 and 2B was determined using the ImageJ Analyze Particle function in the corresponding imaging channels for SIAH2 mRNA signals or DAPI staining of nuclei