Fig. 4
c-Myc up-regulated the expression of Trp transporters and ABC transporters. A, western blotting of c-Myc in LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE). B, the expression of ABCB1, ABCB2, ABCG2, ABCC1, ABCC3, ABCC5, ABCC6 in LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE) detected by real-time PCR. C, the western blotting of P-gp in LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE). D, relative uptake of Dox in LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE). E, cell apoptosis of LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE) treated with Abi (10 μM). F, cell apoptosis of LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE) treated with Doc (1 μg/ml). G, the expression of SLC16A1, SLC22A1, SLC22A3, SLCEA5, SLC7A8, SLC16AE in LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE) detected by real-time PCR. H, Trp concentration in culture medium of LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE). 105 cells were cultured in 2 ml culture medium for 48 h. I, Kyn concentration in culture medium of LNCaP/VCaP cells (VEC) and c-Myc overexpression LNCaP/VCaP cells (OE). 105 cells were cultured in 2 ml culture medium for 48 h