Fig. 5
From: Metformin alters therapeutic effects in the BALB/c tumor therapy model
Cytotoxic effects of chemotherapeutics plus metformin on BALB/c cells. Non-transformed BALB/c cells were seeded in 96 well plates and allowed to grow confluent for 48 h. Afterwards, cells were treated for 24 h with different concentrations of a Doxorubicin, b Docetaxel, c Mitomycin C or d 5-Fluorouracil alone or in combination with different concentrations of metformin. Cell viability was measured indirectly by the reduction of resazurin to fluorescent resorufin. Data are shown as mean + SD of 3 biological replicates. Statistical differences were calculated with a one-way ANOVA (post-hoc: two sided Dunnett-T or Dunnett-T3) with * = (p < 0.05); ** = (p < 0.01) and *** = (p < 0.001) vs. control and # = (p < 0.05) vs. 10 μM Mitomycin C