Fig. 2
From: Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma
Characterization of human L1 ORF2p antibody (anti-hL1ORF2): a) Immunoblot analysis with anti-hL1ORF2RTEH on induced bacterial lysate obtained from and pEThL1ORF1RRM, pET28A and pET-hL1RTEH. Human L1ORF1p RRM antigen used to make anti-hL1ORF1p didn’t show any cross-reaction with anti-hL1ORF2p [26, 27] (b) Immunoblot analysis with anti-hL1ORF1RRM on total bacterial lysate obtained from pET-hL1RTEH, and pEThL1ORF1RRM. No cross-reaction of anti-hL1ORF1with hL1RTEH fragment used as antigen to make ORF2p antibody [26]. c Detection of L1ORF2p (exogenous), L1ORF1p (endogenous and exogenous) and GAPDH (endogenous) [26, 27] by immunoblotting in HEK 293T cells after transfecting full-length L1 construct (pL1RPEGFP) [39]. All the original immuno-blots are shown in supplementary file