Fig. 4

Normal fibroblasts suppresses MF biomarker expression in MyLa cells: a, b Expression of MF biomarker genes (TWIST1 and TOX) are significantly suppressed in MyLa cells after co-culture with normal fibroblast (TWIST1: p < 0.0006; TOX: p < 0.03) as measured by RT-PCR. Co-culture of MyLa cells with MF fibroblasts does not show effect on MF biomarkers gene expression. Data represented here is the mean of three (n = 3) different experiment and paired t-test has been performed to calculate the p value. c, d Normal fibroblasts promotes Th1 environment in MyLa cells: Expression of Th1 cytokine and marker genes (IFNG and TBX21) are significantly increased in MyLa cells after co-culture with normal fibroblast (IFNG: p < 0.03; TBX21: p < 0.03), whereas co-culture of MyLa cells with MF fibroblasts further suppresses Th1 cytokine and biomarker gene expression in MyLa cells