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Fig. 3 | BMC Cancer

Fig. 3

From: Normal and cancer fibroblasts differentially regulate TWIST1, TOX and cytokine gene expression in cutaneous T-cell lymphoma

Fig. 3

a Expression of fibroblast markers: Fibroblasts separated after co-culture were analyzed for gene expression by RT-PCR to quantify changes in the expression of known fibroblast markers (VIM, HSP47) and a gene which is expressed by both fibroblasts and cancer-associated fibroblasts (ACTA2). b ACTA2 gene expression in normal and MF fibroblasts by RT-PCR (NF: Normal Fibroblast; PF: Patient Fibroblast). c Cellular Senescence: A colorimetric based cellular senescence assay based on quantification of the senescence-associated beta-galactosidase (SA-β-gal or SABG) was performed. Co-culture of fibroblasts with MyLa cells had no effect on fibroblast proliferation. d MF biomarker expression in cell lines: MyLa, HH and Hut78 cell lines were tested for MF biomarkers (TWIST1 and TOX). Jurkat cells were used as a control. Only MyLa cells showed expression of TWIST1 and TOX compared to Jurkat cells

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BMC Cancer

ISSN: 1471-2407

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