Fig. 3
Detection of BRAFV600E mutation in mRNA from clinical FFPE samples. BRAFV600E mRNA based mutation assay was utilized for ultrasensitive detection of the BRAFV600E mutation in mRNA isolated from clinical FFPE specimens of thyroid cancer and non-malignant thyroid disease. a Amplification signals from a sample containing mutant BRAFV600E mRNA (B7020 - red line), a sample without mutant BRAFV600E mRNA (B6659 - blue line) and no-template control (NTC - green line); b Corresponding melting peaks of the amplification products