Fig. 7

The enhancement of the growth inhibition after combined treatment of sorafenib and PD0332991 in HepG2 cells and Huh7 cells. a Migration assay was performed. Penetrating HepG2 cells and Huh7 cells after treatment with indicated compound for 24 h were fixed and visualized by the staining of crystal violet. b, c Quantitative analyses were performed for the cells migrating through the matrigel-coated filter. Five random fields of each test at × 200 magnifications were counted. d, f Analysis of apoptosis by Annexin V-APC/propidium iodide (PI) double staining of HepG2 and Huh7 cells after 24 h treatment with indicated compound. Two-color flow cytometry dot plots show the percentages of living cells as negative for both annexin V and PI; early-stage apoptotic cells as the populations testing Annexin V positive and PI negative, and late-stage apoptotic/necrotic cells as double-positive cells. Results are represented in as mean ± SD, n = 3. Statistical analyses were performed using GraphPad Prism. Results are expressed as mean ± SE (range). Comparisons between groups were made using the Mann-Whitney test.*, P < 0.05;**, P < 0.01;;***, P < 0.001