- Research article
- Open Access
- Open Peer Review
Trabectedin arrests a doxorubicin-resistant PDGFRA-activated liposarcoma patient-derived orthotopic xenograft (PDOX) nude mouse model
- Tasuku Kiyuna1, 2, 3,
- Yasunori Tome3Email author,
- Takashi Murakami1, 2,
- Kei Kawaguchi1, 2,
- Kentaro Igarashi1, 2,
- Kentaro Miyake1, 2,
- Masuyo Miyake1, 2,
- Yunfeng Li5,
- Scott D. Nelson5,
- Sarah M. Dry5,
- Arun S. Singh4,
- Tara A. Russell6,
- Irmina Elliott6,
- Shree Ram Singh7Email authorView ORCID ID profile,
- Fuminori Kanaya3,
- Fritz C. Eilber6Email author and
- Robert M. Hoffman1, 2Email author
© The Author(s). 2018
- Received: 6 February 2018
- Accepted: 30 July 2018
- Published: 20 August 2018
Pleomorphic liposarcoma (PLPS) is a rare, heterogeneous and an aggressive variant of liposarcoma. Therefore, individualized therapy is urgently needed. Our recent reports suggest that trabectedin (TRAB) is effective against several patient-derived orthotopic xenograft (PDOX) mouse models. Here, we compared the efficacy of first-line therapy, doxorubicin (DOX), and TRAB in a platelet-derived growth factor receptor-α (PDGFRA)-amplified PLPS.
We used a fresh sample of PLPS tumor derived from a 68-year-old male patient diagnosed with a recurrent PLPS. Subcutaneous implantation of tumor tissue was performed in a nude mouse. After three weeks of implantation, tumor tissues were isolated and cut into small pieces. To match the patient a PDGFRA-amplified PLPS PDOX was created in the biceps femoris of nude mice. Mice were randomized into three groups: Group 1 (G1), control (untreated); Group 2 (G2), DOX-treated; Group 3 (G3), TRAB-treated. Measurement was done twice a week for tumor width, length, and mouse body weight.
The PLPS PDOX showed resistance towards DOX. However, TRAB could arrest the PLPS (p < 0.05 compared to control; p < 0.05 compared to DOX) without any significant changes in body-weight.
The data presented here suggest that for the individual patient the PLPS PDOX model could specifically distinguish both effective and ineffective drugs. This is especially crucial for PLPS because effective first-line therapy is harder to establish if it is not individualized.
- Patient-derived orthotopic xenograft
- PDGFRA amplification
- Precision medicine
Pleomorphic liposarcoma (PLPS), a type of sarcoma, is a rare and an aggressive variant of liposarcoma. PLPS is a recalcitrant disease. Patients with PLPS develop an elevated level of local recurrence and distant metastasis with poor prognosis . PLPS consists of approximately 10% of liposarcomas. PLPS has been investigated in soft tissue sarcomas (STS) such as head and neck sarcoma as well as bone sarcoma [2, 3] and has been demonstrated in patients of all ages [4–7]. Wang et al.  reported 6 PLPS cases out of a total 89 liposarcoma cases between 2003 and 2017. All 6 patients underwent complete tumor resection and only one patient received chemotherapy with ifosfamide and epirubicin . A primary PLPS in an 18-year-old male in the metaphysis of the left tibia was reported by Tiemeier et al. . The patient was treated with methotrexate, doxorubicin and cisplatinum (MAP). Pathology results demonstrated extensive (> 95%) tumor necrosis due to neoadjuvant chemotherapy. Chemotherapy and post-operative results after 12 months showed no sign of recurrence . Yan et al.  have shown an 81-year-old Chinese woman with advanced PLPS who was treated with apatinib after failure of chemotherapy had good efficacy and low toxicity. Apatinib was also used in patients with advanced sarcoma . In search of molecular biomarkers, Ghadimi et al.  tested 155 PLPS patients using tissue microarrays, and identified several potential therapeutic targets .
Surgical resection at present is the only effective therapeutic option for localized PLPS. In addition, radiation and chemotherapy are largely ineffective for advanced stages of this disease [11–14]. Thus, for patients, personalised and targeted therapy is necessary to overcome the metastatic PLPS.
To accomplish this goal, our laboratory has developed the patient-derived orthotopic xenograft (PDOX) nude- mouse model for many tumor types [15–49]. Our PDOX model is advantageous compared to subcutaneous (s.c.)-transplanted patient-derived xenograft (PDX) models in various aspects [50, 51]. In contrast to PDOX model, s.c.-transplanted PDX models fail to develop an advanced tumor stage and cannot retain the original disease pattern generally observed in patients, PDOX models metastasize because tumor tissues are engrafted in the orthotopic sites of origin [50–52]. Importantly, the metastasic form of PDOX model matches the patient. Even though high technical skill, time commitment, and more costly procedures are needed for the PDOX models compared to traditional subcutaneous PDX models, PDOX models are important for individual patients and can be used as a powerful tool in preclinical modelling [50–52].
Trabectedin (TRAB) for patients with metastatic liposarcoma has been approved by the FDA  and is marketed by Janssen Pharma as Yondelis . TRAB is a tetrahydroisoquinoline alkaloid compound, derived from the Carribean sea tunicate, Ecteinascidia turbinate [7, 55]. TRAB is a promising antitumor agent [56–59]. Nteli et al.  reported a durable response to TRAB in a patient with high-grade uterine leiomyosarcoma. It was reported that cells lacking a homologous recombination system were more sensitive to TRAB . Subsequently, Larsen et al.  found that an interaction between a minor groove (DNA), and transcription factors, or DNA-repair molecules such as BRCA1, with TRAB alters the cell cycle and induces cell death. TRAB also showed anti-inflammatory and immunomodulatory properties . Several studies reported that a haplotype in the BRCA1 gene could be utilized as a marker for predicting TRAB effectiveness in patients with STS [63–65]. Angarita et al.  reported some efficacy of TRAB to advanced STS patients who did not respond to first-line chemotherapy.
Recently, we showed that TRAB is efficacious on several PDOX models [25, 30, 32, 67]. Here, we tested the efficacy of first-line chemotherapy, doxorubicin (DOX) , and TRAB in a PDGFRA-amplified  PLPS PDOX model .
In the present study, athymic nu/nu nude mice, between 4 and 6 weeks old, were utilised . Experimental procedures and data collection were done as per as our previous publications [22, 24, 30, 32, 38, 47, 69, 70]. Mouse housing, feeding, surgical processes and imaging were conducted as described in our previous publications [22, 24, 30, 32, 38, 47, 69, 70]. The mice were humanely sacrificed as described in our previous publications [22, 24, 30, 32, 38, 47, 69, 70]. The mouse investigations presented here were done using an AntiCancer, Inc. Institutional Animal Care and Use Committee (IACUC)-protocol specifically approved for this study as previously described  and as per as principles and procedures provided in the National Institute of Health (NIH) Guide for the Care and Use of Animals under Assurance Number A3873–1 .
Patient-derived PLPS tumor
In this study, we used a PLPS tumor derived from a 68-year-old male patient diagnosed with a recurrent PLPS, which has been described in our previous publication . Details of surgical resection and chemotherapy given to this patient have been previously described .
Establishing the PLPS PDOX model using the surgical orthotopic implantation (SOI) technique
PLPS sample collection from the patient, performing subcutaneous implantation in nude mice, harvesting tumors from the mice, creating a space at the orthotopic site in the biceps femoris to insert tumor fragments in the mice and to establish the PDOX model and wound-closure procedures have been described in detail in our previous publications [22, 24, 30, 32, 38, 47, 69].
All treatment procedures and data collection were done as previously reported [22, 24, 30, 32, 38, 47]. PLPS PDOX mouse models were randomized into three groups as previously described : Group 1 (G1), control (untreated); Group 2 (G2), DOX-treated (3 mg/kg, i.v., weekly for 2 weeks); Group 3 (G3), TRAB-treated (0.15 mg/kg, i.v., weekly for 2 weeks). In each group, 7 mice were used. Measurement of tumor width, length, and mouse body weight was done as described in our previous publications [22, 24, 30, 32, 38, 47, 69]. The doses and treatment time were selected from our previous PDOX studies [22, 24, 30, 32, 38, 47, 69]. DOX was selected because it is first-line therapy for PLPS.
A formula to calculate the tumor volume has been previously described . All data are presented in the results section as mean ± SD. Drug treatment was started only when the tumor volume attained 50 mm3 . The tumor volume ratio and the actual body weight was measured as defined in our previous publications [22, 24, 30, 32, 38, 47, 69]. Mice were sacrificed on day 15 in each drug-treatment group and tumors were resected for further histological analysis as described in our previous publication .
All statistical analyses were done using JMP pro version 12 . The relative tumor volumes and relative body weight of the mice are presented as mean ± SD . The Mann-Whitney U test was used to confirm the significant differences for continuous variables. P values of less than 0.05 were regarded as statistically significant.
Drug efficacy in the PLPS PDOX mouse model
Figure 3b shows representative images of tumors harvested from the biceps femoris at the termination point on day 15. The control group had the largest tumors and the TRAB groups had the smallest tumors (Fig. 3b).
Effect of drug treatment on body weight
Effect of drug treatment on tumor histology
First-line systemic therapy for PLPS with an anthracycline, such as a DOX-containing regimen, has a low response rate  consistent with our results presented here. We found that the PLPS PDOX showed resistance to first-line therapy DOX, which is similar to our previous study . In contrast, the PLPS PDOX was arrested by TRAB. This suggests that PLPS PDOX model could specifically recognize both effective and ineffective drugs for each patient .
TRAB is a novel marine-derived alkaloid [7, 73]. It attaches covalently to the DNA minor groove and interacts with transcription factors . Recently, Pignochino et al.  demonstrated TRAB and Poly [ADP-ribose] polymerase 1 (PARP1 1) inhibition synergism in sarcomas. Laroche et al.  tested the efficacy of a combination of TRAB and rucaparib on STS and found that they were also synergistic, enhanced apoptosis and blocked the cell cycle. In addition, these combinations were more effective than TRAB and rucaparib alone . Further, they observed that the combination of these two drugs resulted in elevated γH2AX intranuclear accumulation, which is due to DNA-damage induction . In vivo results further demonstrated that combining these two drugs significantly improved disease-free survival with massive tumor necrosis . A few studies also showed that TRAB has anti-tumor and anti-inflammatory activities [77, 78] and can selectively lower monocytes, tumor-associated macrophages (TAM), and angiogenesis . It has also been demonstrated that the therapeutic efficacy of TRAB in osteosarcoma is increased in combination with a PD-1-blocking antibody .
TRAB has been demonstrated as a therapeutic option in STS [56, 57, 80–86], recurrent ovarian cancer , metastatic breast cancer , solitary fibrous tumor (SFT)-PDXs , desmoplastic small round cell tumor , juvenile myelomonocytic leukaemia and chronic myelomonocytic leukaemia . Recently, we reported that TRAB is efficacious on an osteosarcoma cisplatinum-resistant lung metastasis , a BRAF-V600E mutated melanoma [30, 32] and a gemcitabine (GEM)-resistant pancreatic cancer .
Here we showed that TRAB was highly effective against a PDOX model of PLPS with a PDGFRA activating mutation  in comparison to DOX. The above results together suggest the improved clinical prospect of PLPS and the importance of individualized therapy with PDOX models . Experiments will be performed in the future to compare TRAB with other first-line therapies of PLPS such as docetaxel and ifosfamide. Whether TRAB will be effective against unresetable PLPS is an experimental question since drug sensitivity is highly specific for each patient. It will be possible to answer this question in the future with a PDOX model derived from a core-needle biopsy.
This manuscript is dedicated to the memory of Dr. A.R. Moossa and Dr. Sun Lee.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
TK and RMH was involved in study conception and design. TK, YT, TM, KK, KI, KM, MM, YL, SDN, SMD, ASS, TAR, IE, SRS, FK, FCE, and RMH were involved in analysis and interpretation of data. TK and RMH were involved in drafting of manuscript. SRS was involved in critical revision of manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
Written informed consent was obtained from the patient as part of a University of California, Los Angeles (UCLA) Institutional Review Board (IRB#10–001857) approved protocol. Written informed consent was obtained from the patient for publication of the study. All mice investigations were done with an AntiCancer, Inc. Institutional Animal Care and Use Committee (IACUC)-protocol mainly approved for this study and in correspondence with the principals and procedures outlined in the National Institute of Health (NIH) Guide for the Care and Use of Animals under Assurance Number A3873–1.
Consent for publication
Written informed consent was obtained from the patient for publication of the study.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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