Correction to: Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM
© The Author(s). 2018
Received: 13 February 2018
Accepted: 13 February 2018
Published: 8 March 2018
The original article was published in BMC Cancer 2018 18:167
In the original publication of this article , published on 8 February 2018, it was noticed that the acknowledgement of the source of the drug ADI-PEG20 was missing. In this Correction, the source of drug ADI-PEG20 is shown (and marked bold). This addition is made in the Methods section, under the heading ADI-PEG20 treatment.
SNB19 and U87 cells, cultured in DMEM + 10% FBS and normal human astrocytes, cultured in speciality media provided by lonza were seeded in replicates (n = 12) at 8 × 104 cells per well in 6-well dishes (Corning, NY, USA). 24 h post seeding, cells were washed with phosphate buffered saline (PBS) and cultured in the presence or absence of ADI-PEG20 (kindly provided by Polaris Pharmaceuticals Inc, San Diego, CA) (1 μg/ml) in media containing, 1 mM citrulline and 10% fetal FBS. ADI- PEG20 was added at the start of the experiment and no fresh media was added to any of the experimental plates before harvesting. ADI-PEG20 treated and untreated media (n = 3) was included for normalization purposes. 48 h after ADI-PEG20 treatment replicate samples for each condition (n = 3) were harvested, collecting both spent media and cells for GC-TOFMS metabolomic ana- lysis. Additional replicates (n = 3) of each condition were collected for total cell count determination.
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