Fig. 8

ROS production and the effect of their inhibition upon I-CRP-treatment of HeLa cells. a ROS levels were measured by flow cytometry through DCFDA staining in cells left alone or pretreated with NAC or QVD.oph and then treated with I-CRP (1.25 U/mL), etoposide (100 μM), or H₂O₂ (100 μM) for 24 h. b The effect on cell viability of cells left alone or pretreated with NAC and then treated with I-CRP (1.25 U/mL), etoposide (100 μM), or H₂O₂ (100 μM) for 24 h, was analyzed by flow cytometry through Annexin-V staining. The results were analyzed using FlowJo software and graphed